Amino acid dipepetide frequency for Gordonia phage Spooky

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.93AlaAla: 14.93 ± 1.19
0.94AlaCys: 0.94 ± 0.248
7.686AlaAsp: 7.686 ± 0.794
7.299AlaGlu: 7.299 ± 0.716
3.373AlaPhe: 3.373 ± 0.535
9.069AlaGly: 9.069 ± 0.997
1.88AlaHis: 1.88 ± 0.393
6.359AlaIle: 6.359 ± 0.675
3.65AlaLys: 3.65 ± 0.419
9.29AlaLeu: 9.29 ± 0.65
3.318AlaMet: 3.318 ± 0.5
3.318AlaAsn: 3.318 ± 0.408
4.921AlaPro: 4.921 ± 0.541
4.147AlaGln: 4.147 ± 0.487
7.908AlaArg: 7.908 ± 0.729
5.143AlaSer: 5.143 ± 0.48
6.636AlaThr: 6.636 ± 0.813
7.299AlaVal: 7.299 ± 0.841
2.101AlaTrp: 2.101 ± 0.549
2.378AlaTyr: 2.378 ± 0.351
0.0AlaXaa: 0.0 ± 0.0
Cys
1.051CysAla: 1.051 ± 0.276
0.221CysCys: 0.221 ± 0.129
1.051CysAsp: 1.051 ± 0.243
1.272CysGlu: 1.272 ± 0.315
0.166CysPhe: 0.166 ± 0.106
1.714CysGly: 1.714 ± 0.401
0.664CysHis: 0.664 ± 0.209
0.387CysIle: 0.387 ± 0.158
0.332CysLys: 0.332 ± 0.16
0.94CysLeu: 0.94 ± 0.251
0.111CysMet: 0.111 ± 0.074
0.332CysAsn: 0.332 ± 0.156
0.719CysPro: 0.719 ± 0.243
0.332CysGln: 0.332 ± 0.141
1.051CysArg: 1.051 ± 0.293
1.051CysSer: 1.051 ± 0.362
0.719CysThr: 0.719 ± 0.196
0.94CysVal: 0.94 ± 0.268
0.608CysTrp: 0.608 ± 0.228
0.332CysTyr: 0.332 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
7.797AspAla: 7.797 ± 0.6
0.829AspCys: 0.829 ± 0.248
4.203AspAsp: 4.203 ± 0.651
4.203AspGlu: 4.203 ± 0.491
1.272AspPhe: 1.272 ± 0.241
5.917AspGly: 5.917 ± 0.881
1.548AspHis: 1.548 ± 0.335
3.097AspIle: 3.097 ± 0.435
1.659AspLys: 1.659 ± 0.315
5.917AspLeu: 5.917 ± 0.585
1.327AspMet: 1.327 ± 0.283
1.825AspAsn: 1.825 ± 0.341
3.926AspPro: 3.926 ± 0.604
2.046AspGln: 2.046 ± 0.413
5.751AspArg: 5.751 ± 0.587
3.318AspSer: 3.318 ± 0.384
4.092AspThr: 4.092 ± 0.577
5.806AspVal: 5.806 ± 0.558
1.382AspTrp: 1.382 ± 0.237
1.825AspTyr: 1.825 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
7.576GluAla: 7.576 ± 0.862
1.051GluCys: 1.051 ± 0.313
3.428GluAsp: 3.428 ± 0.409
2.654GluGlu: 2.654 ± 0.479
1.935GluPhe: 1.935 ± 0.35
3.539GluGly: 3.539 ± 0.61
1.659GluHis: 1.659 ± 0.273
2.157GluIle: 2.157 ± 0.383
2.544GluLys: 2.544 ± 0.461
5.751GluLeu: 5.751 ± 0.791
1.051GluMet: 1.051 ± 0.22
1.327GluAsn: 1.327 ± 0.25
2.82GluPro: 2.82 ± 0.505
2.267GluGln: 2.267 ± 0.287
4.811GluArg: 4.811 ± 0.513
2.931GluSer: 2.931 ± 0.377
3.428GluThr: 3.428 ± 0.423
5.253GluVal: 5.253 ± 0.62
1.548GluTrp: 1.548 ± 0.299
0.885GluTyr: 0.885 ± 0.236
0.0GluXaa: 0.0 ± 0.0
Phe
2.433PheAla: 2.433 ± 0.338
0.221PheCys: 0.221 ± 0.122
1.88PheAsp: 1.88 ± 0.305
1.161PheGlu: 1.161 ± 0.266
0.664PhePhe: 0.664 ± 0.174
2.322PheGly: 2.322 ± 0.399
0.995PheHis: 0.995 ± 0.19
0.829PheIle: 0.829 ± 0.221
0.829PheLys: 0.829 ± 0.198
1.88PheLeu: 1.88 ± 0.382
0.885PheMet: 0.885 ± 0.325
1.106PheAsn: 1.106 ± 0.264
1.327PhePro: 1.327 ± 0.263
0.885PheGln: 0.885 ± 0.194
1.659PheArg: 1.659 ± 0.343
1.106PheSer: 1.106 ± 0.267
2.212PheThr: 2.212 ± 0.335
1.825PheVal: 1.825 ± 0.354
0.774PheTrp: 0.774 ± 0.205
0.774PheTyr: 0.774 ± 0.238
0.0PheXaa: 0.0 ± 0.0
Gly
7.963GlyAla: 7.963 ± 1.297
1.161GlyCys: 1.161 ± 0.323
5.364GlyAsp: 5.364 ± 0.505
3.981GlyGlu: 3.981 ± 0.494
2.82GlyPhe: 2.82 ± 0.473
7.244GlyGly: 7.244 ± 1.084
1.77GlyHis: 1.77 ± 0.395
4.977GlyIle: 4.977 ± 0.922
2.986GlyLys: 2.986 ± 0.455
5.53GlyLeu: 5.53 ± 0.441
2.322GlyMet: 2.322 ± 0.363
2.433GlyAsn: 2.433 ± 0.37
3.539GlyPro: 3.539 ± 0.474
3.152GlyGln: 3.152 ± 0.363
5.53GlyArg: 5.53 ± 0.53
5.585GlySer: 5.585 ± 0.931
4.756GlyThr: 4.756 ± 0.566
6.138GlyVal: 6.138 ± 0.52
2.488GlyTrp: 2.488 ± 0.341
2.433GlyTyr: 2.433 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
1.77HisAla: 1.77 ± 0.283
0.664HisCys: 0.664 ± 0.202
1.493HisAsp: 1.493 ± 0.287
1.106HisGlu: 1.106 ± 0.242
0.553HisPhe: 0.553 ± 0.171
2.212HisGly: 2.212 ± 0.404
0.829HisHis: 0.829 ± 0.263
1.217HisIle: 1.217 ± 0.334
0.664HisLys: 0.664 ± 0.283
2.212HisLeu: 2.212 ± 0.397
0.608HisMet: 0.608 ± 0.165
0.608HisAsn: 0.608 ± 0.174
1.548HisPro: 1.548 ± 0.313
0.885HisGln: 0.885 ± 0.233
1.548HisArg: 1.548 ± 0.319
1.382HisSer: 1.382 ± 0.296
1.548HisThr: 1.548 ± 0.367
3.207HisVal: 3.207 ± 0.742
0.442HisTrp: 0.442 ± 0.168
0.387HisTyr: 0.387 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
5.64IleAla: 5.64 ± 0.597
0.498IleCys: 0.498 ± 0.176
3.318IleAsp: 3.318 ± 0.509
3.263IleGlu: 3.263 ± 0.543
0.94IlePhe: 0.94 ± 0.261
4.203IleGly: 4.203 ± 0.623
1.217IleHis: 1.217 ± 0.259
1.382IleIle: 1.382 ± 0.324
1.493IleLys: 1.493 ± 0.663
3.207IleLeu: 3.207 ± 0.351
0.442IleMet: 0.442 ± 0.157
1.272IleAsn: 1.272 ± 0.286
3.097IlePro: 3.097 ± 0.425
1.327IleGln: 1.327 ± 0.264
4.037IleArg: 4.037 ± 0.419
2.71IleSer: 2.71 ± 0.317
2.765IleThr: 2.765 ± 0.339
3.041IleVal: 3.041 ± 0.377
0.664IleTrp: 0.664 ± 0.205
1.161IleTyr: 1.161 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
3.65LysAla: 3.65 ± 0.627
0.387LysCys: 0.387 ± 0.181
2.157LysAsp: 2.157 ± 0.427
1.604LysGlu: 1.604 ± 0.279
0.885LysPhe: 0.885 ± 0.221
2.101LysGly: 2.101 ± 0.5
0.885LysHis: 0.885 ± 0.23
1.548LysIle: 1.548 ± 0.266
1.991LysLys: 1.991 ± 0.438
2.82LysLeu: 2.82 ± 0.371
0.553LysMet: 0.553 ± 0.173
1.106LysAsn: 1.106 ± 0.341
1.991LysPro: 1.991 ± 0.371
1.051LysGln: 1.051 ± 0.308
2.875LysArg: 2.875 ± 0.448
2.212LysSer: 2.212 ± 0.443
2.433LysThr: 2.433 ± 0.536
2.488LysVal: 2.488 ± 0.33
0.94LysTrp: 0.94 ± 0.231
0.664LysTyr: 0.664 ± 0.174
0.0LysXaa: 0.0 ± 0.0
Leu
9.732LeuAla: 9.732 ± 0.83
1.051LeuCys: 1.051 ± 0.287
5.419LeuAsp: 5.419 ± 0.633
3.981LeuGlu: 3.981 ± 0.576
1.493LeuPhe: 1.493 ± 0.302
5.917LeuGly: 5.917 ± 0.677
1.659LeuHis: 1.659 ± 0.298
3.428LeuIle: 3.428 ± 0.491
2.931LeuLys: 2.931 ± 0.373
5.309LeuLeu: 5.309 ± 0.706
2.046LeuMet: 2.046 ± 0.277
1.935LeuAsn: 1.935 ± 0.309
4.756LeuPro: 4.756 ± 0.581
2.82LeuGln: 2.82 ± 0.47
5.309LeuArg: 5.309 ± 0.586
4.203LeuSer: 4.203 ± 0.378
4.866LeuThr: 4.866 ± 0.64
5.087LeuVal: 5.087 ± 0.592
1.77LeuTrp: 1.77 ± 0.294
1.217LeuTyr: 1.217 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
2.986MetAla: 2.986 ± 0.476
0.166MetCys: 0.166 ± 0.093
0.94MetAsp: 0.94 ± 0.205
0.719MetGlu: 0.719 ± 0.182
0.442MetPhe: 0.442 ± 0.159
1.77MetGly: 1.77 ± 0.324
0.276MetHis: 0.276 ± 0.143
0.719MetIle: 0.719 ± 0.186
0.719MetLys: 0.719 ± 0.202
1.106MetLeu: 1.106 ± 0.272
0.332MetMet: 0.332 ± 0.125
0.719MetAsn: 0.719 ± 0.217
1.327MetPro: 1.327 ± 0.257
0.774MetGln: 0.774 ± 0.223
1.991MetArg: 1.991 ± 0.311
2.82MetSer: 2.82 ± 0.403
3.097MetThr: 3.097 ± 0.475
1.327MetVal: 1.327 ± 0.298
0.498MetTrp: 0.498 ± 0.174
0.332MetTyr: 0.332 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
3.373AsnAla: 3.373 ± 0.428
0.719AsnCys: 0.719 ± 0.207
1.604AsnAsp: 1.604 ± 0.285
1.659AsnGlu: 1.659 ± 0.333
0.774AsnPhe: 0.774 ± 0.213
3.428AsnGly: 3.428 ± 0.472
1.106AsnHis: 1.106 ± 0.243
1.106AsnIle: 1.106 ± 0.29
0.332AsnLys: 0.332 ± 0.115
2.378AsnLeu: 2.378 ± 0.42
0.332AsnMet: 0.332 ± 0.13
0.608AsnAsn: 0.608 ± 0.175
2.378AsnPro: 2.378 ± 0.269
0.774AsnGln: 0.774 ± 0.233
2.544AsnArg: 2.544 ± 0.481
1.438AsnSer: 1.438 ± 0.296
2.267AsnThr: 2.267 ± 0.341
1.88AsnVal: 1.88 ± 0.375
0.332AsnTrp: 0.332 ± 0.105
0.829AsnTyr: 0.829 ± 0.257
0.0AsnXaa: 0.0 ± 0.0
Pro
6.083ProAla: 6.083 ± 0.53
0.94ProCys: 0.94 ± 0.272
4.7ProAsp: 4.7 ± 0.582
4.756ProGlu: 4.756 ± 0.553
1.106ProPhe: 1.106 ± 0.302
4.534ProGly: 4.534 ± 0.534
1.438ProHis: 1.438 ± 0.332
2.433ProIle: 2.433 ± 0.378
2.157ProLys: 2.157 ± 0.406
2.82ProLeu: 2.82 ± 0.362
1.659ProMet: 1.659 ± 0.292
2.322ProAsn: 2.322 ± 0.394
2.544ProPro: 2.544 ± 0.466
1.604ProGln: 1.604 ± 0.317
2.82ProArg: 2.82 ± 0.48
3.152ProSer: 3.152 ± 0.483
3.65ProThr: 3.65 ± 0.466
3.373ProVal: 3.373 ± 0.471
1.106ProTrp: 1.106 ± 0.278
1.272ProTyr: 1.272 ± 0.28
0.0ProXaa: 0.0 ± 0.0
Gln
4.203GlnAla: 4.203 ± 0.526
0.553GlnCys: 0.553 ± 0.173
1.438GlnAsp: 1.438 ± 0.258
1.327GlnGlu: 1.327 ± 0.239
1.327GlnPhe: 1.327 ± 0.251
2.378GlnGly: 2.378 ± 0.333
0.664GlnHis: 0.664 ± 0.168
2.101GlnIle: 2.101 ± 0.339
0.829GlnLys: 0.829 ± 0.204
3.318GlnLeu: 3.318 ± 0.517
1.106GlnMet: 1.106 ± 0.272
0.829GlnAsn: 0.829 ± 0.256
1.77GlnPro: 1.77 ± 0.291
1.77GlnGln: 1.77 ± 0.303
3.097GlnArg: 3.097 ± 0.402
2.157GlnSer: 2.157 ± 0.336
2.101GlnThr: 2.101 ± 0.39
2.82GlnVal: 2.82 ± 0.434
0.664GlnTrp: 0.664 ± 0.191
1.161GlnTyr: 1.161 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
7.355ArgAla: 7.355 ± 0.75
0.94ArgCys: 0.94 ± 0.295
6.138ArgAsp: 6.138 ± 0.539
5.087ArgGlu: 5.087 ± 0.555
1.327ArgPhe: 1.327 ± 0.237
5.087ArgGly: 5.087 ± 0.577
2.046ArgHis: 2.046 ± 0.404
3.65ArgIle: 3.65 ± 0.535
3.373ArgLys: 3.373 ± 0.487
5.143ArgLeu: 5.143 ± 0.535
2.101ArgMet: 2.101 ± 0.387
2.71ArgAsn: 2.71 ± 0.393
3.926ArgPro: 3.926 ± 0.504
3.097ArgGln: 3.097 ± 0.399
6.746ArgArg: 6.746 ± 0.899
4.037ArgSer: 4.037 ± 0.586
4.313ArgThr: 4.313 ± 0.496
4.7ArgVal: 4.7 ± 0.538
2.212ArgTrp: 2.212 ± 0.403
1.659ArgTyr: 1.659 ± 0.329
0.0ArgXaa: 0.0 ± 0.0
Ser
5.253SerAla: 5.253 ± 0.59
0.94SerCys: 0.94 ± 0.291
3.705SerAsp: 3.705 ± 0.516
3.428SerGlu: 3.428 ± 0.416
1.438SerPhe: 1.438 ± 0.249
6.249SerGly: 6.249 ± 0.859
1.88SerHis: 1.88 ± 0.844
2.101SerIle: 2.101 ± 0.289
2.157SerLys: 2.157 ± 0.306
3.428SerLeu: 3.428 ± 0.381
1.88SerMet: 1.88 ± 0.318
1.438SerAsn: 1.438 ± 0.294
2.654SerPro: 2.654 ± 0.363
1.714SerGln: 1.714 ± 0.317
3.926SerArg: 3.926 ± 0.562
3.428SerSer: 3.428 ± 0.547
4.369SerThr: 4.369 ± 0.55
4.424SerVal: 4.424 ± 0.664
1.217SerTrp: 1.217 ± 0.277
0.94SerTyr: 0.94 ± 0.259
0.0SerXaa: 0.0 ± 0.0
Thr
7.078ThrAla: 7.078 ± 0.719
0.664ThrCys: 0.664 ± 0.223
4.313ThrAsp: 4.313 ± 0.611
3.263ThrGlu: 3.263 ± 0.493
2.157ThrPhe: 2.157 ± 0.342
5.917ThrGly: 5.917 ± 0.617
1.493ThrHis: 1.493 ± 0.302
3.373ThrIle: 3.373 ± 0.56
1.548ThrLys: 1.548 ± 0.289
4.479ThrLeu: 4.479 ± 0.469
0.774ThrMet: 0.774 ± 0.183
1.825ThrAsn: 1.825 ± 0.26
4.645ThrPro: 4.645 ± 0.485
2.71ThrGln: 2.71 ± 0.406
4.424ThrArg: 4.424 ± 0.56
3.539ThrSer: 3.539 ± 0.433
5.032ThrThr: 5.032 ± 0.774
5.751ThrVal: 5.751 ± 0.527
1.327ThrTrp: 1.327 ± 0.383
1.604ThrTyr: 1.604 ± 0.344
0.0ThrXaa: 0.0 ± 0.0
Val
8.516ValAla: 8.516 ± 0.994
1.217ValCys: 1.217 ± 0.298
4.7ValAsp: 4.7 ± 0.488
5.253ValGlu: 5.253 ± 0.718
1.714ValPhe: 1.714 ± 0.261
5.253ValGly: 5.253 ± 0.556
1.825ValHis: 1.825 ± 0.324
2.986ValIle: 2.986 ± 0.409
2.599ValLys: 2.599 ± 0.549
5.585ValLeu: 5.585 ± 0.552
1.438ValMet: 1.438 ± 0.313
2.433ValAsn: 2.433 ± 0.397
3.871ValPro: 3.871 ± 0.451
2.378ValGln: 2.378 ± 0.375
5.862ValArg: 5.862 ± 0.926
4.037ValSer: 4.037 ± 0.464
4.369ValThr: 4.369 ± 0.583
5.972ValVal: 5.972 ± 0.537
2.157ValTrp: 2.157 ± 0.384
1.604ValTyr: 1.604 ± 0.349
0.0ValXaa: 0.0 ± 0.0
Trp
1.991TrpAla: 1.991 ± 0.283
0.553TrpCys: 0.553 ± 0.158
2.322TrpAsp: 2.322 ± 0.465
1.272TrpGlu: 1.272 ± 0.245
0.608TrpPhe: 0.608 ± 0.176
1.382TrpGly: 1.382 ± 0.34
0.553TrpHis: 0.553 ± 0.2
1.327TrpIle: 1.327 ± 0.273
0.553TrpLys: 0.553 ± 0.173
2.157TrpLeu: 2.157 ± 0.386
0.276TrpMet: 0.276 ± 0.121
0.995TrpAsn: 0.995 ± 0.261
1.106TrpPro: 1.106 ± 0.256
1.106TrpGln: 1.106 ± 0.2
2.322TrpArg: 2.322 ± 0.4
1.106TrpSer: 1.106 ± 0.243
1.604TrpThr: 1.604 ± 0.297
0.995TrpVal: 0.995 ± 0.225
0.608TrpTrp: 0.608 ± 0.202
0.332TrpTyr: 0.332 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.267TyrAla: 2.267 ± 0.422
0.276TyrCys: 0.276 ± 0.106
1.88TyrAsp: 1.88 ± 0.306
1.327TyrGlu: 1.327 ± 0.281
0.774TyrPhe: 0.774 ± 0.222
1.935TyrGly: 1.935 ± 0.316
0.498TyrHis: 0.498 ± 0.15
0.498TyrIle: 0.498 ± 0.16
0.995TyrLys: 0.995 ± 0.281
1.659TyrLeu: 1.659 ± 0.318
0.442TyrMet: 0.442 ± 0.142
0.719TyrAsn: 0.719 ± 0.194
1.604TyrPro: 1.604 ± 0.339
0.774TyrGln: 0.774 ± 0.249
1.438TyrArg: 1.438 ± 0.293
1.217TyrSer: 1.217 ± 0.301
1.659TyrThr: 1.659 ± 0.33
1.493TyrVal: 1.493 ± 0.302
0.332TyrTrp: 0.332 ± 0.148
0.387TyrTyr: 0.387 ± 0.152
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (18085 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski