Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540)
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6WV09|C6WV09_METML Uncharacterized protein OS=Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540) OX=583345 GN=Mmol_0848 PE=4 SV=1
MM1 pKa = 7.48 ALIGKK6 pKa = 8.81 IIAMTGTASLISNNGNQRR24 pKa = 11.84 DD25 pKa = 4.01 LRR27 pKa = 11.84 LGDD30 pKa = 4.37 NIQTADD36 pKa = 4.0 TIKK39 pKa = 9.69 TGAGVEE45 pKa = 4.08 VDD47 pKa = 3.67 LQLANGQVVHH57 pKa = 7.02 IGAEE61 pKa = 3.99 QLVAFADD68 pKa = 3.88 EE69 pKa = 4.34 LVEE72 pKa = 4.54 DD73 pKa = 5.74 FIPATLDD80 pKa = 3.1 TSVNVATIDD89 pKa = 3.78 TVVKK93 pKa = 10.57 AIEE96 pKa = 4.08 EE97 pKa = 4.33 GKK99 pKa = 10.59 DD100 pKa = 2.94 IGDD103 pKa = 3.76 VLEE106 pKa = 4.44 EE107 pKa = 4.03 TAAGPGGSSNSYY119 pKa = 9.86 GFSFVDD125 pKa = 3.93 LLRR128 pKa = 11.84 INDD131 pKa = 3.93 DD132 pKa = 3.65 LNNFRR137 pKa = 11.84 FGYY140 pKa = 9.6 EE141 pKa = 4.14 FGSATPNEE149 pKa = 4.2 QALSGNSFADD159 pKa = 3.34 TLNQINVVTGTGGAPDD175 pKa = 4.01 TNSPSVVVNIVDD187 pKa = 3.67 ANLNVADD194 pKa = 4.16 TVSNVTFTFSEE205 pKa = 4.6 APVGFTIGDD214 pKa = 3.37 ISSTGGIISNLVATADD230 pKa = 3.85 PLVFTATFTANAGFEE245 pKa = 4.54 GTGSVSVNAGSYY257 pKa = 9.55 TNASGNVGTSGADD270 pKa = 3.08 TVGIDD275 pKa = 3.36 TAAPVPTITLTPNVTADD292 pKa = 4.06 DD293 pKa = 4.6 IINAGEE299 pKa = 3.88 AGGNVNITGTVGGDD313 pKa = 2.91 AKK315 pKa = 11.58 VNDD318 pKa = 4.13 TVTLVINGNTYY329 pKa = 9.85 TGLVQPGLTFSIPVAGSDD347 pKa = 4.57 LVADD351 pKa = 4.45 PDD353 pKa = 3.89 RR354 pKa = 11.84 VIDD357 pKa = 4.08 ASVSTTDD364 pKa = 3.63 AAGNTATASDD374 pKa = 4.0 TEE376 pKa = 4.74 GYY378 pKa = 10.15 SVDD381 pKa = 3.54 STVPTIANQTFSYY394 pKa = 10.76 AEE396 pKa = 3.76 NRR398 pKa = 11.84 AAGATVATVVASDD411 pKa = 3.54 NVGVTGYY418 pKa = 10.88 SFTATGTNTSADD430 pKa = 3.41 GYY432 pKa = 8.73 YY433 pKa = 10.11 QISNTGVITITAAGAASGVNDD454 pKa = 4.43 FEE456 pKa = 5.1 QGTNTGNYY464 pKa = 9.24 GITVRR469 pKa = 11.84 DD470 pKa = 3.3 AAGNNSNATITLNEE484 pKa = 4.07 TDD486 pKa = 5.47 LDD488 pKa = 3.89 DD489 pKa = 4.5 TNPVIANQTFSYY501 pKa = 10.76 AEE503 pKa = 3.76 NRR505 pKa = 11.84 AAGATVATVVASDD518 pKa = 3.54 NVGVTGYY525 pKa = 10.88 SFTATGTNTSADD537 pKa = 3.41 GYY539 pKa = 8.73 YY540 pKa = 10.11 QISNTGVITITAAGAASGVNDD561 pKa = 4.43 FEE563 pKa = 5.1 QGTNTGNYY571 pKa = 9.24 GITVRR576 pKa = 11.84 DD577 pKa = 3.3 AAGNNSNATITLNEE591 pKa = 4.25 TNVNEE596 pKa = 3.96 PVAAVSDD603 pKa = 4.45 AYY605 pKa = 10.0 TMNEE609 pKa = 4.33 DD610 pKa = 3.63 GPAITLTPLANDD622 pKa = 3.81 SDD624 pKa = 4.89 PDD626 pKa = 3.64 GTTPTIQSINGTALTPGVAQAIAVTGGMVNVSAAGVITFTPTLNFNGTVTFPYY679 pKa = 10.36 VITDD683 pKa = 4.07 GATTATANQVITVNAVDD700 pKa = 4.61 DD701 pKa = 4.48 PSVLIADD708 pKa = 4.11 TNTVNEE714 pKa = 4.77 DD715 pKa = 3.15 NPATGNVLSNDD726 pKa = 3.29 SDD728 pKa = 3.66 VDD730 pKa = 3.66 NVLTVSSFKK739 pKa = 10.88 VAGISGTFTAGQTATITGVGTLTIAANGNYY769 pKa = 9.01 TFTPVANYY777 pKa = 9.91 NGSVPVATYY786 pKa = 8.82 TVNTGSTSTLSITVTPVNDD805 pKa = 3.72 APVGVADD812 pKa = 4.07 VFNGVNSVVEE822 pKa = 4.15 GTTVVRR828 pKa = 11.84 GNILANDD835 pKa = 3.95 TDD837 pKa = 3.85 VDD839 pKa = 4.26 STTLTVAQFATNSGATATAVNGTNSITTALGGTVVMNADD878 pKa = 3.3 GTFTYY883 pKa = 7.9 TAPVRR888 pKa = 11.84 NHH890 pKa = 6.55 ADD892 pKa = 3.73 AISDD896 pKa = 3.27 VDD898 pKa = 3.97 SFVYY902 pKa = 10.3 RR903 pKa = 11.84 ATDD906 pKa = 3.57 GSLNSAWTTVTLNIADD922 pKa = 4.25 SVPVANPDD930 pKa = 3.4 TDD932 pKa = 3.84 SVGVGFRR939 pKa = 11.84 NPTTNNTASVTGNVITGAGDD959 pKa = 3.2 TGGVDD964 pKa = 3.43 TLGADD969 pKa = 3.7 AARR972 pKa = 11.84 VSSIVFNGTTYY983 pKa = 11.29 NLSAGNTSIVTSNGTLLINEE1003 pKa = 4.54 TGSYY1007 pKa = 9.6 TYY1009 pKa = 10.42 TSSYY1013 pKa = 8.19 QNKK1016 pKa = 8.23 VVPVSPSNTASTVANWTSAGISSFGFDD1043 pKa = 3.08 GTSPLTNSNNTLTLSALTTAASNIVRR1069 pKa = 11.84 LSDD1072 pKa = 3.38 NTNSNNDD1079 pKa = 3.94 GIGVEE1084 pKa = 4.48 TTLSGSASNQSNISRR1099 pKa = 11.84 IEE1101 pKa = 4.08 NNEE1104 pKa = 3.9 HH1105 pKa = 6.54 LVLNLGMLSRR1115 pKa = 11.84 NTSVTLTDD1123 pKa = 3.82 LTASEE1128 pKa = 4.31 TAQWRR1133 pKa = 11.84 AYY1135 pKa = 9.19 DD1136 pKa = 3.6 ANGAIVASGTITGNSTNIATGTVSSTVAFQYY1167 pKa = 10.64 IVFSGSSSANFRR1179 pKa = 11.84 VNGLTATPDD1188 pKa = 3.54 LTAVTPDD1195 pKa = 2.85 QFTYY1199 pKa = 10.78 TLTDD1203 pKa = 3.44 ADD1205 pKa = 4.34 GSTASTTLTINTNGNPSAVADD1226 pKa = 3.66 SATVYY1231 pKa = 10.71 EE1232 pKa = 4.81 SGLSTGTQAGVLATTASGNLLVNDD1256 pKa = 4.8 AGVSTTTTISSINGVTPTSGTITVTSATGTLVVNANTGAYY1296 pKa = 8.22 TYY1298 pKa = 9.38 TLNAATTEE1306 pKa = 4.19 GVNDD1310 pKa = 3.96 KK1311 pKa = 9.18 PTFNYY1316 pKa = 10.29 VLTDD1320 pKa = 3.51 SVTGQSTNANLTVNIVDD1337 pKa = 4.23 DD1338 pKa = 4.82 APVGGNITQTLQAASAALTYY1358 pKa = 10.91 NVVIVLDD1365 pKa = 4.22 RR1366 pKa = 11.84 SGSMAQDD1373 pKa = 3.95 ANGLWSNQSGYY1384 pKa = 10.63 DD1385 pKa = 3.17 ASTNRR1390 pKa = 11.84 MEE1392 pKa = 4.27 IAKK1395 pKa = 9.79 EE1396 pKa = 3.91 AIAQLIARR1404 pKa = 11.84 YY1405 pKa = 9.86 DD1406 pKa = 3.39 GLGNVNVKK1414 pKa = 9.95 FVTFSSDD1421 pKa = 2.98 AVEE1424 pKa = 4.33 SEE1426 pKa = 4.06 WYY1428 pKa = 9.39 IDD1430 pKa = 3.59 NVTGAVRR1437 pKa = 11.84 YY1438 pKa = 9.54 VDD1440 pKa = 3.69 NVQAGGGTQYY1450 pKa = 10.0 STALNEE1456 pKa = 4.26 TMSGFTQPVADD1467 pKa = 3.44 KK1468 pKa = 10.02 TLFYY1472 pKa = 10.46 FITDD1476 pKa = 4.05 GQPNSGYY1483 pKa = 10.39 EE1484 pKa = 3.87 VDD1486 pKa = 5.13 ATLQTQWQNFVAANGNISFGIGIGTASLSSLTPIAYY1522 pKa = 9.22 PNVDD1526 pKa = 3.02 ADD1528 pKa = 4.18 GNGTEE1533 pKa = 5.01 DD1534 pKa = 3.4 YY1535 pKa = 10.6 AIRR1538 pKa = 11.84 VDD1540 pKa = 4.2 NPADD1544 pKa = 3.65 LADD1547 pKa = 4.13 TLLSTVDD1554 pKa = 3.29 GGVVVGNMSVLSGSGSSGFLLGADD1578 pKa = 3.9 GGRR1581 pKa = 11.84 LNSVVVDD1588 pKa = 4.16 GVTYY1592 pKa = 9.21 TYY1594 pKa = 10.66 SASGPSSMTISTNKK1608 pKa = 10.24 GGEE1611 pKa = 4.04 LTVNFLTGEE1620 pKa = 4.08 YY1621 pKa = 9.93 SYY1623 pKa = 11.6 HH1624 pKa = 6.58 LLLNRR1629 pKa = 11.84 TVQNEE1634 pKa = 4.1 QEE1636 pKa = 4.5 SFLVTAVDD1644 pKa = 3.76 GDD1646 pKa = 4.23 GDD1648 pKa = 4.04 TKK1650 pKa = 10.85 TINLNINLDD1659 pKa = 3.81 YY1660 pKa = 11.02 VANLDD1665 pKa = 4.02 PNADD1669 pKa = 3.98 TILTNVVTGTPISVSAAALLHH1690 pKa = 6.05 NDD1692 pKa = 3.52 SLRR1695 pKa = 11.84 GTGSITSTQNAVNGSVSGTSTVQFTANAVAAKK1727 pKa = 8.33 TIQVVTEE1734 pKa = 3.99 ASFDD1738 pKa = 3.94 STSQTQNSTRR1748 pKa = 11.84 EE1749 pKa = 4.0 NAVDD1753 pKa = 3.35 LTDD1756 pKa = 3.52 RR1757 pKa = 11.84 SKK1759 pKa = 11.2 FGTVVSGTPTWAVDD1773 pKa = 3.49 VAGGSSIVFSGLLDD1787 pKa = 3.55 NSGSGTRR1794 pKa = 11.84 DD1795 pKa = 2.64 VDD1797 pKa = 3.82 YY1798 pKa = 11.52 VKK1800 pKa = 11.12 VKK1802 pKa = 9.21 MYY1804 pKa = 10.46 AGEE1807 pKa = 4.09 RR1808 pKa = 11.84 VFIDD1812 pKa = 4.19 VDD1814 pKa = 3.58 NQTQGVNAFVEE1825 pKa = 4.46 YY1826 pKa = 10.46 FDD1828 pKa = 5.65 ANGVLQTFTVGTTGTGTSLAPNGYY1852 pKa = 8.02 FTAPEE1857 pKa = 3.9 SGEE1860 pKa = 3.88 YY1861 pKa = 9.55 FIRR1864 pKa = 11.84 MQTVGTTDD1872 pKa = 2.79 TSYY1875 pKa = 11.96 NLVLTLTDD1883 pKa = 2.9 IVGPMTEE1890 pKa = 4.29 NGSFEE1895 pKa = 4.3 YY1896 pKa = 10.26 TVTEE1900 pKa = 3.95 NGVASSASATVYY1912 pKa = 10.5 NVVGNTITGGDD1923 pKa = 3.65 GDD1925 pKa = 4.83 EE1926 pKa = 4.6 ILLGGNTNDD1935 pKa = 3.64 TLIGGGGNDD1944 pKa = 3.64 VLIGGAGNDD1953 pKa = 3.71 TLLGGTGADD1962 pKa = 3.34 RR1963 pKa = 11.84 LEE1965 pKa = 4.64 GGSGNDD1971 pKa = 3.32 ILNGGTGNDD1980 pKa = 3.21 ILIGGTGNDD1989 pKa = 3.89 TLTGGLGADD1998 pKa = 3.47 VFKK2001 pKa = 10.84 WSLADD2006 pKa = 3.89 AGATGTPATDD2016 pKa = 3.57 VITDD2020 pKa = 4.32 FDD2022 pKa = 3.84 TTANSDD2028 pKa = 3.72 KK2029 pKa = 11.21 LDD2031 pKa = 4.69 LRR2033 pKa = 11.84 DD2034 pKa = 4.03 LLQGEE2039 pKa = 4.48 IGSGVGANLEE2049 pKa = 4.27 NYY2051 pKa = 10.32 LHH2053 pKa = 6.44 FEE2055 pKa = 4.12 KK2056 pKa = 10.91 VGTDD2060 pKa = 3.39 TVVHH2064 pKa = 6.59 ISSNGSFNNGYY2075 pKa = 9.73 NPAAEE2080 pKa = 4.3 VQTITLQNVDD2090 pKa = 4.11 LVGSYY2095 pKa = 11.52 ANDD2098 pKa = 3.22 QQVIQNLINNQKK2110 pKa = 10.63 LITDD2114 pKa = 4.23
Molecular weight: 214.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.567
IPC_protein 3.643
Toseland 3.389
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.3
Solomon 3.643
Lehninger 3.592
Nozaki 3.745
DTASelect 4.05
Thurlkill 3.452
EMBOSS 3.605
Sillero 3.757
Patrickios 1.291
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|C6WTX1|C6WTX1_METML Uncharacterized protein OS=Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540) OX=583345 GN=Mmol_0460 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.4 TKK25 pKa = 10.36 GGRR28 pKa = 11.84 AVIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LGLL44 pKa = 3.62
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2334
0
2334
757536
23
2787
324.6
35.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.12 ± 0.061
0.868 ± 0.019
5.273 ± 0.041
5.659 ± 0.046
3.809 ± 0.035
6.82 ± 0.058
2.329 ± 0.029
6.3 ± 0.044
5.375 ± 0.044
10.506 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.618 ± 0.025
4.353 ± 0.046
4.033 ± 0.032
4.371 ± 0.038
4.635 ± 0.037
6.22 ± 0.039
5.517 ± 0.046
7.202 ± 0.041
1.127 ± 0.021
2.864 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here