Jhaorihella thermophila
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3696 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H5ZFW9|A0A1H5ZFW9_9RHOB S-methyl-5'-thioadenosine phosphorylase OS=Jhaorihella thermophila OX=488547 GN=mtnP PE=3 SV=1
MM1 pKa = 7.9 PLTFEE6 pKa = 4.2 TLRR9 pKa = 11.84 IILPYY14 pKa = 10.57 AVILAAIDD22 pKa = 4.57 LIEE25 pKa = 4.44 SLLTLLYY32 pKa = 10.81 LGFANLPPSPPLAEE46 pKa = 4.83 GDD48 pKa = 3.75 DD49 pKa = 3.88 GAVYY53 pKa = 10.07 GVV55 pKa = 3.45
Molecular weight: 5.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.903
IPC2_protein 3.77
IPC_protein 3.49
Toseland 3.363
ProMoST 3.745
Dawson 3.528
Bjellqvist 3.706
Wikipedia 3.528
Rodwell 3.376
Grimsley 3.3
Solomon 3.414
Lehninger 3.376
Nozaki 3.719
DTASelect 3.783
Thurlkill 3.478
EMBOSS 3.528
Sillero 3.643
Patrickios 0.299
IPC_peptide 3.414
IPC2_peptide 3.579
IPC2.peptide.svr19 3.663
Protein with the highest isoelectric point:
>tr|A0A1H5V9J0|A0A1H5V9J0_9RHOB Corrinoid adenosyltransferase OS=Jhaorihella thermophila OX=488547 GN=SAMN05421751_105198 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 VLSAA44 pKa = 4.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3696
0
3696
1135509
28
2158
307.2
33.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.738 ± 0.059
0.906 ± 0.014
6.036 ± 0.036
6.177 ± 0.041
3.6 ± 0.022
8.774 ± 0.04
2.11 ± 0.019
4.94 ± 0.027
2.919 ± 0.034
9.939 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.695 ± 0.019
2.337 ± 0.018
5.445 ± 0.031
2.911 ± 0.023
7.955 ± 0.042
4.479 ± 0.023
5.103 ± 0.028
7.335 ± 0.032
1.486 ± 0.018
2.117 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here