Macaca mulatta papillomavirus 2
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385AHQ9|A0A385AHQ9_9PAPI Protein E6 OS=Macaca mulatta papillomavirus 2 OX=2294150 GN=E6 PE=3 SV=1
MM1 pKa = 7.33 HH2 pKa = 7.29 GKK4 pKa = 10.24 APTLKK9 pKa = 10.49 EE10 pKa = 3.88 IILNLEE16 pKa = 4.17 PEE18 pKa = 4.43 PVNLQCQEE26 pKa = 4.04 VLEE29 pKa = 5.41 DD30 pKa = 5.2 SDD32 pKa = 6.3 DD33 pKa = 5.82 DD34 pKa = 5.1 DD35 pKa = 5.16 DD36 pKa = 5.97 QVDD39 pKa = 3.85 ATQPTQPARR48 pKa = 11.84 QAYY51 pKa = 8.85 KK52 pKa = 10.47 VLCQCAGGCCKK63 pKa = 10.13 SVRR66 pKa = 11.84 LVVYY70 pKa = 7.47 STKK73 pKa = 10.77 EE74 pKa = 3.99 GIQRR78 pKa = 11.84 LHH80 pKa = 7.2 DD81 pKa = 4.48 LLVDD85 pKa = 3.83 DD86 pKa = 5.63 ALNIVCPTCACKK98 pKa = 10.39 LL99 pKa = 3.66
Molecular weight: 10.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.218
IPC2_protein 4.546
IPC_protein 4.431
Toseland 4.253
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.329
Rodwell 4.266
Grimsley 4.164
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.736
Thurlkill 4.291
EMBOSS 4.342
Sillero 4.558
Patrickios 2.028
IPC_peptide 4.406
IPC2_peptide 4.533
IPC2.peptide.svr19 4.476
Protein with the highest isoelectric point:
>tr|A0A385AH66|A0A385AH66_9PAPI Replication protein E1 OS=Macaca mulatta papillomavirus 2 OX=2294150 GN=E1 PE=3 SV=1
MM1 pKa = 7.44 VMQKK5 pKa = 9.67 IACHH9 pKa = 5.3 MLCGLTFMCIQRR21 pKa = 11.84 TYY23 pKa = 10.68 GQRR26 pKa = 11.84 CLVGSMQGVCTMTRR40 pKa = 11.84 RR41 pKa = 11.84 ARR43 pKa = 11.84 GCTMRR48 pKa = 11.84 TLLTMQTCMGLMNNGKK64 pKa = 9.89 CMWAATT70 pKa = 3.91
Molecular weight: 7.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.923
IPC2_protein 8.551
IPC_protein 8.565
Toseland 8.258
ProMoST 8.96
Dawson 9.107
Bjellqvist 9.545
Wikipedia 9.033
Rodwell 9.136
Grimsley 7.907
Solomon 9.355
Lehninger 9.37
Nozaki 9.911
DTASelect 9.194
Thurlkill 9.268
EMBOSS 9.326
Sillero 9.619
Patrickios 2.6
IPC_peptide 9.341
IPC2_peptide 9.531
IPC2.peptide.svr19 8.912
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2305
70
642
329.3
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.896 ± 0.55
2.993 ± 0.978
5.857 ± 0.505
5.249 ± 0.578
3.688 ± 0.625
6.681 ± 0.482
2.56 ± 0.386
3.948 ± 0.251
4.729 ± 0.778
7.896 ± 0.505
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.733 ± 0.625
3.991 ± 0.538
6.464 ± 1.415
4.772 ± 0.547
5.9 ± 0.557
6.768 ± 0.474
6.508 ± 0.584
6.508 ± 0.372
1.432 ± 0.336
3.427 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here