Pseudonocardiaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; unclassified Pseudonocardiaceae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7HZS1|A0A6N7HZS1_9PSEU XRE family transcriptional regulator OS=Pseudonocardiaceae bacterium OX=1873463 GN=GEU98_18655 PE=4 SV=1
MM1 pKa = 7.49TDD3 pKa = 2.75ALRR6 pKa = 11.84RR7 pKa = 11.84FSRR10 pKa = 11.84LVGIGAATLLAGLGTVSVADD30 pKa = 3.88AAPAKK35 pKa = 9.69PADD38 pKa = 3.64VGADD42 pKa = 2.95IVGGQDD48 pKa = 4.21ADD50 pKa = 3.37IADD53 pKa = 3.95YY54 pKa = 10.14PFTVALTTPNGEE66 pKa = 4.17QFCGGTLAAANKK78 pKa = 9.57VVTAAHH84 pKa = 6.57CMQGEE89 pKa = 4.34SPEE92 pKa = 4.0NLRR95 pKa = 11.84VVSGRR100 pKa = 11.84TAMSSNEE107 pKa = 3.9GTVSEE112 pKa = 4.84VSDD115 pKa = 3.09IWVHH119 pKa = 6.98PDD121 pKa = 3.28YY122 pKa = 11.35QDD124 pKa = 3.01ATQGFDD130 pKa = 3.3VAVVTLAGNVQEE142 pKa = 4.78QPIEE146 pKa = 4.08LATADD151 pKa = 3.6DD152 pKa = 4.11PGYY155 pKa = 11.21NEE157 pKa = 4.08GTEE160 pKa = 3.99ATILGWGTTSEE171 pKa = 4.43GGNTSDD177 pKa = 4.35TLQQANVPVNSDD189 pKa = 3.39QDD191 pKa = 3.62CSSAYY196 pKa = 9.91QEE198 pKa = 4.34YY199 pKa = 10.8NPDD202 pKa = 3.36AMVCAGLPEE211 pKa = 4.88GGVDD215 pKa = 3.58SCQGDD220 pKa = 3.14SGGPMVAGGKK230 pKa = 10.1LIGVTSWGEE239 pKa = 3.73GCARR243 pKa = 11.84PGKK246 pKa = 8.82PGIYY250 pKa = 9.77ARR252 pKa = 11.84VGAYY256 pKa = 9.92HH257 pKa = 7.53DD258 pKa = 4.36VLTEE262 pKa = 3.86PLGGSSADD270 pKa = 3.38ARR272 pKa = 4.0

Molecular weight: 27.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7HS22|A0A6N7HS22_9PSEU Protein meaA OS=Pseudonocardiaceae bacterium OX=1873463 GN=GEU98_06005 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.65HH19 pKa = 4.47GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.7GRR42 pKa = 11.84ARR44 pKa = 11.84LSAA47 pKa = 3.91

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5656

0

5656

1789655

28

5437

316.4

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.652 ± 0.038

0.852 ± 0.01

5.95 ± 0.031

6.023 ± 0.031

2.9 ± 0.021

9.285 ± 0.033

2.274 ± 0.018

3.754 ± 0.022

1.985 ± 0.023

10.412 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.893 ± 0.012

2.042 ± 0.017

5.686 ± 0.028

2.899 ± 0.022

8.002 ± 0.034

5.386 ± 0.022

5.728 ± 0.019

8.706 ± 0.031

1.502 ± 0.013

2.068 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski