Amino acid dipepetide frequency for Pseudonocardiaceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.709AlaAla: 17.709 ± 0.15	1.052AlaCys: 1.052 ± 0.022	7.661AlaAsp: 7.661 ± 0.077	9.439AlaGlu: 9.439 ± 0.09	3.37AlaPhe: 3.37 ± 0.045	12.822AlaGly: 12.822 ± 0.095	2.481AlaHis: 2.481 ± 0.037	4.73AlaIle: 4.73 ± 0.058	2.828AlaLys: 2.828 ± 0.05	12.954AlaLeu: 12.954 ± 0.107	2.642AlaMet: 2.642 ± 0.037	2.462AlaAsn: 2.462 ± 0.042	5.641AlaPro: 5.641 ± 0.064	3.281AlaGln: 3.281 ± 0.049	9.543AlaArg: 9.543 ± 0.092	6.319AlaSer: 6.319 ± 0.057	6.744AlaThr: 6.744 ± 0.056	11.044AlaVal: 11.044 ± 0.093	1.623AlaTrp: 1.623 ± 0.036	2.17AlaTyr: 2.17 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.127CysAla: 1.127 ± 0.027	0.115CysCys: 0.115 ± 0.007	0.487CysAsp: 0.487 ± 0.019	0.482CysGlu: 0.482 ± 0.017	0.261CysPhe: 0.261 ± 0.012	1.052CysGly: 1.052 ± 0.024	0.193CysHis: 0.193 ± 0.011	0.198CysIle: 0.198 ± 0.011	0.097CysLys: 0.097 ± 0.007	0.752CysLeu: 0.752 ± 0.019	0.121CysMet: 0.121 ± 0.008	0.126CysAsn: 0.126 ± 0.009	0.503CysPro: 0.503 ± 0.018	0.226CysGln: 0.226 ± 0.012	0.652CysArg: 0.652 ± 0.02	0.462CysSer: 0.462 ± 0.017	0.555CysThr: 0.555 ± 0.016	0.739CysVal: 0.739 ± 0.02	0.167CysTrp: 0.167 ± 0.009	0.201CysTyr: 0.201 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.262AspAla: 7.262 ± 0.08	0.42AspCys: 0.42 ± 0.015	3.724AspAsp: 3.724 ± 0.044	4.554AspGlu: 4.554 ± 0.055	1.526AspPhe: 1.526 ± 0.027	6.036AspGly: 6.036 ± 0.072	1.191AspHis: 1.191 ± 0.026	2.1AspIle: 2.1 ± 0.036	1.097AspLys: 1.097 ± 0.03	5.541AspLeu: 5.541 ± 0.061	0.887AspMet: 0.887 ± 0.022	1.12AspAsn: 1.12 ± 0.026	4.2AspPro: 4.2 ± 0.049	1.663AspGln: 1.663 ± 0.033	4.58AspArg: 4.58 ± 0.053	2.728AspSer: 2.728 ± 0.041	3.071AspThr: 3.071 ± 0.04	5.549AspVal: 5.549 ± 0.06	0.939AspTrp: 0.939 ± 0.023	1.316AspTyr: 1.316 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.652GluAla: 6.652 ± 0.075	0.42GluCys: 0.42 ± 0.018	2.698GluAsp: 2.698 ± 0.039	3.001GluGlu: 3.001 ± 0.046	1.9GluPhe: 1.9 ± 0.032	3.677GluGly: 3.677 ± 0.049	2.051GluHis: 2.051 ± 0.034	2.608GluIle: 2.608 ± 0.038	1.237GluLys: 1.237 ± 0.034	8.518GluLeu: 8.518 ± 0.079	1.08GluMet: 1.08 ± 0.024	1.185GluAsn: 1.185 ± 0.026	3.712GluPro: 3.712 ± 0.05	2.839GluGln: 2.839 ± 0.044	5.918GluArg: 5.918 ± 0.067	2.922GluSer: 2.922 ± 0.044	2.854GluThr: 2.854 ± 0.043	4.888GluVal: 4.888 ± 0.052	0.852GluTrp: 0.852 ± 0.023	1.222GluTyr: 1.222 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.834PheAla: 3.834 ± 0.049	0.312PheCys: 0.312 ± 0.014	2.162PheAsp: 2.162 ± 0.036	1.489PheGlu: 1.489 ± 0.031	0.958PhePhe: 0.958 ± 0.029	3.553PheGly: 3.553 ± 0.054	0.636PheHis: 0.636 ± 0.016	0.85PheIle: 0.85 ± 0.025	0.412PheLys: 0.412 ± 0.017	2.664PheLeu: 2.664 ± 0.044	0.428PheMet: 0.428 ± 0.018	0.622PheAsn: 0.622 ± 0.021	1.401PhePro: 1.401 ± 0.028	0.697PheGln: 0.697 ± 0.018	1.844PheArg: 1.844 ± 0.04	1.611PheSer: 1.611 ± 0.034	1.905PheThr: 1.905 ± 0.035	2.566PheVal: 2.566 ± 0.037	0.439PheTrp: 0.439 ± 0.017	0.615PheTyr: 0.615 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	10.461GlyAla: 10.461 ± 0.097	0.912GlyCys: 0.912 ± 0.025	4.747GlyAsp: 4.747 ± 0.055	5.579GlyGlu: 5.579 ± 0.058	3.128GlyPhe: 3.128 ± 0.044	8.295GlyGly: 8.295 ± 0.094	2.069GlyHis: 2.069 ± 0.035	3.844GlyIle: 3.844 ± 0.044	2.532GlyLys: 2.532 ± 0.042	9.179GlyLeu: 9.179 ± 0.072	2.325GlyMet: 2.325 ± 0.037	1.993GlyAsn: 1.993 ± 0.042	4.372GlyPro: 4.372 ± 0.05	3.098GlyGln: 3.098 ± 0.048	6.777GlyArg: 6.777 ± 0.071	5.5GlySer: 5.5 ± 0.054	5.586GlyThr: 5.586 ± 0.06	8.042GlyVal: 8.042 ± 0.08	1.819GlyTrp: 1.819 ± 0.034	2.595GlyTyr: 2.595 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.725HisAla: 2.725 ± 0.047	0.229HisCys: 0.229 ± 0.01	1.381HisAsp: 1.381 ± 0.029	1.313HisGlu: 1.313 ± 0.03	0.622HisPhe: 0.622 ± 0.02	2.49HisGly: 2.49 ± 0.039	0.646HisHis: 0.646 ± 0.023	0.731HisIle: 0.731 ± 0.02	0.354HisLys: 0.354 ± 0.014	2.114HisLeu: 2.114 ± 0.036	0.324HisMet: 0.324 ± 0.013	0.474HisAsn: 0.474 ± 0.014	1.672HisPro: 1.672 ± 0.031	0.674HisGln: 0.674 ± 0.021	1.985HisArg: 1.985 ± 0.039	1.034HisSer: 1.034 ± 0.026	1.273HisThr: 1.273 ± 0.028	1.829HisVal: 1.829 ± 0.032	0.36HisTrp: 0.36 ± 0.013	0.511HisTyr: 0.511 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	5.542IleAla: 5.542 ± 0.064	0.35IleCys: 0.35 ± 0.013	2.584IleAsp: 2.584 ± 0.041	2.45IleGlu: 2.45 ± 0.041	0.911IlePhe: 0.911 ± 0.025	4.444IleGly: 4.444 ± 0.068	0.607IleHis: 0.607 ± 0.018	1.137IleIle: 1.137 ± 0.029	0.739IleLys: 0.739 ± 0.024	2.65IleLeu: 2.65 ± 0.039	0.573IleMet: 0.573 ± 0.02	0.93IleAsn: 0.93 ± 0.023	2.018IlePro: 2.018 ± 0.033	0.789IleGln: 0.789 ± 0.02	2.516IleArg: 2.516 ± 0.045	2.148IleSer: 2.148 ± 0.035	2.624IleThr: 2.624 ± 0.04	3.414IleVal: 3.414 ± 0.052	0.43IleTrp: 0.43 ± 0.016	0.689IleTyr: 0.689 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.319LysAla: 2.319 ± 0.049	0.111LysCys: 0.111 ± 0.008	1.059LysAsp: 1.059 ± 0.028	1.002LysGlu: 1.002 ± 0.028	0.501LysPhe: 0.501 ± 0.019	1.416LysGly: 1.416 ± 0.035	0.492LysHis: 0.492 ± 0.017	0.935LysIle: 0.935 ± 0.028	0.574LysLys: 0.574 ± 0.025	2.205LysLeu: 2.205 ± 0.038	0.386LysMet: 0.386 ± 0.015	0.455LysAsn: 0.455 ± 0.017	1.409LysPro: 1.409 ± 0.036	0.812LysGln: 0.812 ± 0.022	1.595LysArg: 1.595 ± 0.034	1.037LysSer: 1.037 ± 0.028	1.113LysThr: 1.113 ± 0.028	1.768LysVal: 1.768 ± 0.035	0.245LysTrp: 0.245 ± 0.013	0.423LysTyr: 0.423 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.241LeuAla: 15.241 ± 0.109	0.935LeuCys: 0.935 ± 0.024	6.954LeuAsp: 6.954 ± 0.069	5.055LeuGlu: 5.055 ± 0.055	2.645LeuPhe: 2.645 ± 0.044	9.772LeuGly: 9.772 ± 0.082	2.267LeuHis: 2.267 ± 0.038	3.886LeuIle: 3.886 ± 0.055	1.455LeuLys: 1.455 ± 0.034	11.201LeuLeu: 11.201 ± 0.11	1.595LeuMet: 1.595 ± 0.031	1.897LeuAsn: 1.897 ± 0.038	6.119LeuPro: 6.119 ± 0.062	2.044LeuGln: 2.044 ± 0.033	9.168LeuArg: 9.168 ± 0.077	5.682LeuSer: 5.682 ± 0.058	6.013LeuThr: 6.013 ± 0.072	9.249LeuVal: 9.249 ± 0.079	1.233LeuTrp: 1.233 ± 0.028	1.71LeuTyr: 1.71 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.309MetAla: 2.309 ± 0.033	0.17MetCys: 0.17 ± 0.009	0.924MetAsp: 0.924 ± 0.022	0.73MetGlu: 0.73 ± 0.021	0.576MetPhe: 0.576 ± 0.018	1.318MetGly: 1.318 ± 0.03	0.435MetHis: 0.435 ± 0.016	0.811MetIle: 0.811 ± 0.02	0.37MetLys: 0.37 ± 0.014	2.092MetLeu: 2.092 ± 0.035	0.327MetMet: 0.327 ± 0.013	0.468MetAsn: 0.468 ± 0.017	1.251MetPro: 1.251 ± 0.025	0.463MetGln: 0.463 ± 0.017	1.699MetArg: 1.699 ± 0.032	1.449MetSer: 1.449 ± 0.026	1.519MetThr: 1.519 ± 0.028	1.485MetVal: 1.485 ± 0.03	0.228MetTrp: 0.228 ± 0.012	0.304MetTyr: 0.304 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.5AsnAla: 2.5 ± 0.039	0.178AsnCys: 0.178 ± 0.011	1.099AsnAsp: 1.099 ± 0.025	1.107AsnGlu: 1.107 ± 0.029	0.564AsnPhe: 0.564 ± 0.02	1.989AsnGly: 1.989 ± 0.037	0.427AsnHis: 0.427 ± 0.015	0.786AsnIle: 0.786 ± 0.021	0.417AsnLys: 0.417 ± 0.018	1.928AsnLeu: 1.928 ± 0.037	0.356AsnMet: 0.356 ± 0.015	0.481AsnAsn: 0.481 ± 0.017	1.591AsnPro: 1.591 ± 0.033	0.619AsnGln: 0.619 ± 0.02	1.505AsnArg: 1.505 ± 0.027	1.053AsnSer: 1.053 ± 0.025	1.273AsnThr: 1.273 ± 0.03	1.716AsnVal: 1.716 ± 0.03	0.337AsnTrp: 0.337 ± 0.015	0.493AsnTyr: 0.493 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	7.106ProAla: 7.106 ± 0.083	0.356ProCys: 0.356 ± 0.015	4.466ProAsp: 4.466 ± 0.058	4.41ProGlu: 4.41 ± 0.056	1.558ProPhe: 1.558 ± 0.026	5.692ProGly: 5.692 ± 0.07	1.257ProHis: 1.257 ± 0.024	1.924ProIle: 1.924 ± 0.036	1.192ProLys: 1.192 ± 0.027	4.826ProLeu: 4.826 ± 0.053	1.149ProMet: 1.149 ± 0.025	1.225ProAsn: 1.225 ± 0.027	3.236ProPro: 3.236 ± 0.061	1.474ProGln: 1.474 ± 0.031	3.78ProArg: 3.78 ± 0.047	3.179ProSer: 3.179 ± 0.048	2.998ProThr: 2.998 ± 0.039	4.986ProVal: 4.986 ± 0.054	0.875ProTrp: 0.875 ± 0.023	1.176ProTyr: 1.176 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.559GlnAla: 3.559 ± 0.045	0.199GlnCys: 0.199 ± 0.012	1.317GlnAsp: 1.317 ± 0.028	1.297GlnGlu: 1.297 ± 0.026	0.762GlnPhe: 0.762 ± 0.023	2.026GlnGly: 2.026 ± 0.04	0.812GlnHis: 0.812 ± 0.023	1.165GlnIle: 1.165 ± 0.028	0.472GlnLys: 0.472 ± 0.016	3.721GlnLeu: 3.721 ± 0.051	0.487GlnMet: 0.487 ± 0.015	0.543GlnAsn: 0.543 ± 0.02	2.131GlnPro: 2.131 ± 0.043	1.42GlnGln: 1.42 ± 0.039	3.074GlnArg: 3.074 ± 0.051	1.27GlnSer: 1.27 ± 0.028	1.259GlnThr: 1.259 ± 0.028	2.492GlnVal: 2.492 ± 0.035	0.426GlnTrp: 0.426 ± 0.015	0.553GlnTyr: 0.553 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.5ArgAla: 9.5 ± 0.085	0.688ArgCys: 0.688 ± 0.02	4.341ArgAsp: 4.341 ± 0.054	4.968ArgGlu: 4.968 ± 0.062	2.566ArgPhe: 2.566 ± 0.03	5.94ArgGly: 5.94 ± 0.052	2.026ArgHis: 2.026 ± 0.034	3.34ArgIle: 3.34 ± 0.041	1.771ArgLys: 1.771 ± 0.033	8.554ArgLeu: 8.554 ± 0.093	1.919ArgMet: 1.919 ± 0.031	1.624ArgAsn: 1.624 ± 0.032	4.3ArgPro: 4.3 ± 0.057	2.62ArgGln: 2.62 ± 0.04	7.405ArgArg: 7.405 ± 0.09	4.326ArgSer: 4.326 ± 0.053	4.523ArgThr: 4.523 ± 0.05	6.134ArgVal: 6.134 ± 0.055	1.462ArgTrp: 1.462 ± 0.029	2.013ArgTyr: 2.013 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.693SerAla: 6.693 ± 0.076	0.471SerCys: 0.471 ± 0.018	2.866SerAsp: 2.866 ± 0.041	3.086SerGlu: 3.086 ± 0.045	1.673SerPhe: 1.673 ± 0.031	6.119SerGly: 6.119 ± 0.062	0.981SerHis: 0.981 ± 0.022	1.928SerIle: 1.928 ± 0.037	1.124SerLys: 1.124 ± 0.031	4.824SerLeu: 4.824 ± 0.053	1.361SerMet: 1.361 ± 0.026	1.075SerAsn: 1.075 ± 0.025	2.859SerPro: 2.859 ± 0.042	1.389SerGln: 1.389 ± 0.028	3.736SerArg: 3.736 ± 0.046	3.266SerSer: 3.266 ± 0.05	3.52SerThr: 3.52 ± 0.046	4.593SerVal: 4.593 ± 0.052	0.986SerTrp: 0.986 ± 0.024	1.316SerTyr: 1.316 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	7.222ThrAla: 7.222 ± 0.071	0.444ThrCys: 0.444 ± 0.016	3.408ThrAsp: 3.408 ± 0.045	3.774ThrGlu: 3.774 ± 0.045	1.63ThrPhe: 1.63 ± 0.035	6.067ThrGly: 6.067 ± 0.051	1.117ThrHis: 1.117 ± 0.023	2.076ThrIle: 2.076 ± 0.035	1.266ThrLys: 1.266 ± 0.03	5.412ThrLeu: 5.412 ± 0.058	1.167ThrMet: 1.167 ± 0.025	1.156ThrAsn: 1.156 ± 0.026	3.483ThrPro: 3.483 ± 0.049	1.322ThrGln: 1.322 ± 0.026	3.615ThrArg: 3.615 ± 0.042	3.178ThrSer: 3.178 ± 0.045	3.419ThrThr: 3.419 ± 0.054	5.697ThrVal: 5.697 ± 0.068	0.785ThrTrp: 0.785 ± 0.02	1.04ThrTyr: 1.04 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	10.883ValAla: 10.883 ± 0.097	0.77ValCys: 0.77 ± 0.022	5.62ValAsp: 5.62 ± 0.063	4.754ValGlu: 4.754 ± 0.064	2.598ValPhe: 2.598 ± 0.042	7.062ValGly: 7.062 ± 0.073	2.141ValHis: 2.141 ± 0.036	3.353ValIle: 3.353 ± 0.049	1.339ValLys: 1.339 ± 0.03	10.609ValLeu: 10.609 ± 0.1	1.256ValMet: 1.256 ± 0.025	1.842ValAsn: 1.842 ± 0.038	4.924ValPro: 4.924 ± 0.056	2.064ValGln: 2.064 ± 0.033	7.299ValArg: 7.299 ± 0.062	4.766ValSer: 4.766 ± 0.05	4.98ValThr: 4.98 ± 0.051	8.185ValVal: 8.185 ± 0.086	1.097ValTrp: 1.097 ± 0.028	1.515ValTyr: 1.515 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.522TrpAla: 1.522 ± 0.028	0.173TrpCys: 0.173 ± 0.009	0.746TrpAsp: 0.746 ± 0.021	0.678TrpGlu: 0.678 ± 0.019	0.537TrpPhe: 0.537 ± 0.018	0.987TrpGly: 0.987 ± 0.027	0.412TrpHis: 0.412 ± 0.015	0.611TrpIle: 0.611 ± 0.018	0.286TrpLys: 0.286 ± 0.013	1.977TrpLeu: 1.977 ± 0.04	0.316TrpMet: 0.316 ± 0.012	0.349TrpAsn: 0.349 ± 0.016	0.811TrpPro: 0.811 ± 0.021	0.714TrpGln: 0.714 ± 0.021	1.376TrpArg: 1.376 ± 0.032	0.899TrpSer: 0.899 ± 0.022	0.919TrpThr: 0.919 ± 0.022	1.037TrpVal: 1.037 ± 0.027	0.365TrpTrp: 0.365 ± 0.015	0.306TrpTyr: 0.306 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.352TyrAla: 2.352 ± 0.035	0.207TyrCys: 0.207 ± 0.011	1.259TyrAsp: 1.259 ± 0.032	1.066TyrGlu: 1.066 ± 0.024	0.713TyrPhe: 0.713 ± 0.024	2.093TyrGly: 2.093 ± 0.044	0.5TyrHis: 0.5 ± 0.016	0.53TyrIle: 0.53 ± 0.016	0.293TyrLys: 0.293 ± 0.013	2.399TyrLeu: 2.399 ± 0.043	0.23TyrMet: 0.23 ± 0.011	0.394TyrAsn: 0.394 ± 0.016	1.232TyrPro: 1.232 ± 0.032	0.779TyrGln: 0.779 ± 0.021	1.952TyrArg: 1.952 ± 0.037	1.037TyrSer: 1.037 ± 0.025	1.13TyrThr: 1.13 ± 0.031	1.648TyrVal: 1.648 ± 0.027	0.355TyrTrp: 0.355 ± 0.014	0.51TyrTyr: 0.51 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5656 proteins (1789655 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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