Paracoccus sp. 228
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3463 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D6TAR2|A0A0D6TAR2_9RHOB Protein-methionine-sulfoxide reductase catalytic subunit MsrP OS=Paracoccus sp. 228 OX=1192054 GN=msrP PE=3 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.09 KK3 pKa = 10.59 ALIASTALVLTAGIAAADD21 pKa = 3.89 VTISGYY27 pKa = 10.89 GRR29 pKa = 11.84 TGVLYY34 pKa = 10.56 QEE36 pKa = 4.84 GGVRR40 pKa = 11.84 GPIDD44 pKa = 3.64 TNGDD48 pKa = 3.51 APGGLVDD55 pKa = 4.87 ASDD58 pKa = 5.51 AIVQSRR64 pKa = 11.84 LRR66 pKa = 11.84 MNIDD70 pKa = 2.93 ATTSTDD76 pKa = 3.03 QGVDD80 pKa = 3.57 FGGRR84 pKa = 11.84 IRR86 pKa = 11.84 IQWDD90 pKa = 3.02 QGDD93 pKa = 4.56 DD94 pKa = 3.65 EE95 pKa = 4.85 TTVAPGYY102 pKa = 10.73 VYY104 pKa = 9.67 VTASGLTVEE113 pKa = 5.0 IGNSNTAYY121 pKa = 10.68 DD122 pKa = 4.06 SAALLYY128 pKa = 10.25 NSEE131 pKa = 3.83 IGVYY135 pKa = 9.93 SRR137 pKa = 11.84 SFGNSRR143 pKa = 11.84 GDD145 pKa = 3.32 FFAYY149 pKa = 8.89 NTDD152 pKa = 3.82 GYY154 pKa = 8.15 PTLANVVDD162 pKa = 4.18 GAIDD166 pKa = 3.47 RR167 pKa = 11.84 RR168 pKa = 11.84 SDD170 pKa = 3.35 YY171 pKa = 10.08 MGVMVKK177 pKa = 10.39 YY178 pKa = 10.29 AVAGVNLRR186 pKa = 11.84 ASVIDD191 pKa = 3.77 PDD193 pKa = 3.9 QVYY196 pKa = 10.72 DD197 pKa = 4.13 LPTDD201 pKa = 3.64 TSEE204 pKa = 4.48 KK205 pKa = 9.34 EE206 pKa = 4.02 YY207 pKa = 10.96 SVSADD212 pKa = 3.61 YY213 pKa = 11.03 VWNDD217 pKa = 3.07 SVTLSAAYY225 pKa = 9.62 VKK227 pKa = 10.72 NGAGIDD233 pKa = 4.31 DD234 pKa = 3.91 NDD236 pKa = 3.6 QYY238 pKa = 11.76 FVGAEE243 pKa = 3.72 YY244 pKa = 11.08 AFNDD248 pKa = 3.54 KK249 pKa = 10.56 TDD251 pKa = 3.0 IGLLYY256 pKa = 9.8 IDD258 pKa = 4.27 NGEE261 pKa = 4.19 VLGDD265 pKa = 4.07 DD266 pKa = 3.7 SDD268 pKa = 4.03 LGKK271 pKa = 9.27 TITLYY276 pKa = 11.02 GSYY279 pKa = 9.9 EE280 pKa = 4.09 VAPLTTINGYY290 pKa = 8.14 VANNSADD297 pKa = 3.74 SNEE300 pKa = 3.89 SDD302 pKa = 3.24 NAFGIGGAYY311 pKa = 10.22 DD312 pKa = 3.52 LGGAMLAGSVQRR324 pKa = 11.84 GYY326 pKa = 11.26 DD327 pKa = 3.56 EE328 pKa = 6.24 NITADD333 pKa = 3.29 MGVQFNFF340 pKa = 3.6
Molecular weight: 35.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.554
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.77
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.215
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.923
Patrickios 0.82
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A0D6TE65|A0A0D6TE65_9RHOB ABC transporter substrate-binding protein OS=Paracoccus sp. 228 OX=1192054 GN=SY26_06145 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.39 GGRR28 pKa = 11.84 LVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.7 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3463
0
3463
1070115
28
2108
309.0
33.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.467 ± 0.067
0.803 ± 0.011
6.449 ± 0.04
4.904 ± 0.044
3.402 ± 0.027
8.861 ± 0.045
2.106 ± 0.021
5.035 ± 0.029
2.288 ± 0.036
10.372 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.882 ± 0.02
2.206 ± 0.021
5.421 ± 0.028
3.53 ± 0.023
7.513 ± 0.043
4.674 ± 0.027
5.434 ± 0.028
7.257 ± 0.036
1.457 ± 0.02
1.933 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here