Streptomyces sp. NRRL F-6491

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8YQV0|A0A0M8YQV0_9ACTN Uncharacterized protein OS=Streptomyces sp. NRRL F-6491 OX=1519495 GN=ADL06_06855 PE=4 SV=1
MM1 pKa = 7.24TVQDD5 pKa = 3.97EE6 pKa = 4.6APATGAGEE14 pKa = 4.09ALEE17 pKa = 4.16VWIDD21 pKa = 3.41QDD23 pKa = 3.99LCTGDD28 pKa = 5.01GICAQYY34 pKa = 10.9APEE37 pKa = 4.23VFEE40 pKa = 5.9LDD42 pKa = 3.05IDD44 pKa = 3.63GLAYY48 pKa = 10.42VKK50 pKa = 10.8SADD53 pKa = 4.97DD54 pKa = 4.59EE55 pKa = 4.56LLQDD59 pKa = 4.09TGATTPVPLPLLQDD73 pKa = 3.66VVDD76 pKa = 4.35SAKK79 pKa = 10.25EE80 pKa = 3.92CPGDD84 pKa = 4.18CIHH87 pKa = 6.39VRR89 pKa = 11.84RR90 pKa = 11.84VADD93 pKa = 3.43KK94 pKa = 11.16VEE96 pKa = 4.41VYY98 pKa = 10.9GPDD101 pKa = 3.36AEE103 pKa = 4.31

Molecular weight: 10.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8YQK6|A0A0M8YQK6_9ACTN Membrane protein (Fragment) OS=Streptomyces sp. NRRL F-6491 OX=1519495 GN=ADL06_07320 PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84HH4 pKa = 5.97HH5 pKa = 7.18PNHH8 pKa = 6.02FWSPRR13 pKa = 11.84VSKK16 pKa = 10.06RR17 pKa = 11.84TFQPNNRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84AKK28 pKa = 8.7THH30 pKa = 5.15GFRR33 pKa = 11.84LRR35 pKa = 11.84MRR37 pKa = 11.84TRR39 pKa = 11.84AGRR42 pKa = 11.84AILANRR48 pKa = 11.84RR49 pKa = 11.84GKK51 pKa = 10.51GRR53 pKa = 11.84ASLSAA58 pKa = 3.83

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6101

0

6101

1955372

21

6099

320.5

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.907 ± 0.057

0.743 ± 0.01

5.966 ± 0.025

5.945 ± 0.03

2.707 ± 0.019

9.749 ± 0.033

2.263 ± 0.015

2.823 ± 0.022

2.09 ± 0.025

10.459 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.596 ± 0.014

1.66 ± 0.018

6.331 ± 0.03

2.385 ± 0.018

8.311 ± 0.036

4.758 ± 0.02

6.109 ± 0.023

8.675 ± 0.03

1.488 ± 0.013

2.032 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski