Amino acid dipepetide frequency for Streptomyces sp. NRRL F-6491

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.052AlaAla: 22.052 ± 0.17	1.006AlaCys: 1.006 ± 0.026	8.394AlaAsp: 8.394 ± 0.08	9.159AlaGlu: 9.159 ± 0.083	3.649AlaPhe: 3.649 ± 0.048	13.861AlaGly: 13.861 ± 0.112	2.972AlaHis: 2.972 ± 0.044	3.026AlaIle: 3.026 ± 0.042	2.746AlaLys: 2.746 ± 0.057	15.263AlaLeu: 15.263 ± 0.125	2.339AlaMet: 2.339 ± 0.037	1.791AlaAsn: 1.791 ± 0.036	8.135AlaPro: 8.135 ± 0.105	3.187AlaGln: 3.187 ± 0.053	11.087AlaArg: 11.087 ± 0.085	5.811AlaSer: 5.811 ± 0.063	6.903AlaThr: 6.903 ± 0.077	12.875AlaVal: 12.875 ± 0.103	1.909AlaTrp: 1.909 ± 0.032	2.911AlaTyr: 2.911 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.09CysAla: 1.09 ± 0.027	0.084CysCys: 0.084 ± 0.007	0.437CysAsp: 0.437 ± 0.013	0.383CysGlu: 0.383 ± 0.015	0.201CysPhe: 0.201 ± 0.009	0.956CysGly: 0.956 ± 0.024	0.183CysHis: 0.183 ± 0.011	0.126CysIle: 0.126 ± 0.007	0.101CysLys: 0.101 ± 0.007	0.721CysLeu: 0.721 ± 0.023	0.107CysMet: 0.107 ± 0.007	0.117CysAsn: 0.117 ± 0.008	0.462CysPro: 0.462 ± 0.018	0.132CysGln: 0.132 ± 0.009	0.575CysArg: 0.575 ± 0.019	0.404CysSer: 0.404 ± 0.015	0.436CysThr: 0.436 ± 0.016	0.66CysVal: 0.66 ± 0.019	0.121CysTrp: 0.121 ± 0.008	0.138CysTyr: 0.138 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.912AspAla: 7.912 ± 0.081	0.403AspCys: 0.403 ± 0.015	3.461AspAsp: 3.461 ± 0.044	3.983AspGlu: 3.983 ± 0.054	1.664AspPhe: 1.664 ± 0.029	6.624AspGly: 6.624 ± 0.072	1.429AspHis: 1.429 ± 0.033	1.552AspIle: 1.552 ± 0.031	1.123AspLys: 1.123 ± 0.031	6.356AspLeu: 6.356 ± 0.065	0.745AspMet: 0.745 ± 0.018	0.842AspAsn: 0.842 ± 0.024	4.785AspPro: 4.785 ± 0.061	1.277AspGln: 1.277 ± 0.03	5.271AspArg: 5.271 ± 0.058	2.328AspSer: 2.328 ± 0.028	3.115AspThr: 3.115 ± 0.04	4.824AspVal: 4.824 ± 0.049	0.996AspTrp: 0.996 ± 0.027	0.97AspTyr: 0.97 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.882GluAla: 7.882 ± 0.075	0.35GluCys: 0.35 ± 0.014	3.08GluAsp: 3.08 ± 0.044	4.203GluGlu: 4.203 ± 0.064	1.409GluPhe: 1.409 ± 0.029	4.777GluGly: 4.777 ± 0.054	1.548GluHis: 1.548 ± 0.029	2.162GluIle: 2.162 ± 0.038	1.561GluLys: 1.561 ± 0.034	6.914GluLeu: 6.914 ± 0.077	0.842GluMet: 0.842 ± 0.023	1.056GluAsn: 1.056 ± 0.022	3.625GluPro: 3.625 ± 0.054	1.995GluGln: 1.995 ± 0.037	5.947GluArg: 5.947 ± 0.058	2.384GluSer: 2.384 ± 0.04	3.191GluThr: 3.191 ± 0.047	4.617GluVal: 4.617 ± 0.066	0.79GluTrp: 0.79 ± 0.02	1.121GluTyr: 1.121 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.738PheAla: 3.738 ± 0.047	0.264PheCys: 0.264 ± 0.013	1.956PheAsp: 1.956 ± 0.033	1.471PheGlu: 1.471 ± 0.032	0.881PhePhe: 0.881 ± 0.026	3.0PheGly: 3.0 ± 0.042	0.614PheHis: 0.614 ± 0.02	0.55PheIle: 0.55 ± 0.018	0.503PheLys: 0.503 ± 0.018	2.678PheLeu: 2.678 ± 0.047	0.386PheMet: 0.386 ± 0.015	0.526PheAsn: 0.526 ± 0.018	1.378PhePro: 1.378 ± 0.024	0.603PheGln: 0.603 ± 0.017	1.908PheArg: 1.908 ± 0.031	1.41PheSer: 1.41 ± 0.033	1.975PheThr: 1.975 ± 0.031	2.272PheVal: 2.272 ± 0.039	0.425PheTrp: 0.425 ± 0.016	0.537PheTyr: 0.537 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.97GlyAla: 11.97 ± 0.113	0.798GlyCys: 0.798 ± 0.02	5.345GlyAsp: 5.345 ± 0.064	5.47GlyGlu: 5.47 ± 0.059	2.924GlyPhe: 2.924 ± 0.041	9.364GlyGly: 9.364 ± 0.101	2.337GlyHis: 2.337 ± 0.039	3.235GlyIle: 3.235 ± 0.042	2.341GlyLys: 2.341 ± 0.044	9.751GlyLeu: 9.751 ± 0.089	1.847GlyMet: 1.847 ± 0.035	1.718GlyAsn: 1.718 ± 0.037	5.829GlyPro: 5.829 ± 0.075	2.375GlyGln: 2.375 ± 0.041	8.476GlyArg: 8.476 ± 0.076	5.28GlySer: 5.28 ± 0.058	6.795GlyThr: 6.795 ± 0.073	7.747GlyVal: 7.747 ± 0.063	1.716GlyTrp: 1.716 ± 0.035	2.168GlyTyr: 2.168 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.777HisAla: 2.777 ± 0.045	0.206HisCys: 0.206 ± 0.01	1.298HisAsp: 1.298 ± 0.029	1.28HisGlu: 1.28 ± 0.027	0.615HisPhe: 0.615 ± 0.018	2.46HisGly: 2.46 ± 0.038	0.705HisHis: 0.705 ± 0.02	0.545HisIle: 0.545 ± 0.019	0.322HisLys: 0.322 ± 0.013	2.446HisLeu: 2.446 ± 0.04	0.302HisMet: 0.302 ± 0.012	0.331HisAsn: 0.331 ± 0.014	1.793HisPro: 1.793 ± 0.033	0.6HisGln: 0.6 ± 0.02	2.224HisArg: 2.224 ± 0.041	0.905HisSer: 0.905 ± 0.023	1.248HisThr: 1.248 ± 0.025	1.74HisVal: 1.74 ± 0.032	0.386HisTrp: 0.386 ± 0.013	0.448HisTyr: 0.448 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.423IleAla: 4.423 ± 0.053	0.274IleCys: 0.274 ± 0.01	1.966IleAsp: 1.966 ± 0.037	1.838IleGlu: 1.838 ± 0.036	0.574IlePhe: 0.574 ± 0.015	3.317IleGly: 3.317 ± 0.053	0.528IleHis: 0.528 ± 0.016	0.679IleIle: 0.679 ± 0.02	0.651IleLys: 0.651 ± 0.019	2.188IleLeu: 2.188 ± 0.041	0.389IleMet: 0.389 ± 0.017	0.591IleAsn: 0.591 ± 0.019	1.494IlePro: 1.494 ± 0.029	0.597IleGln: 0.597 ± 0.019	2.053IleArg: 2.053 ± 0.033	1.361IleSer: 1.361 ± 0.025	1.946IleThr: 1.946 ± 0.037	2.596IleVal: 2.596 ± 0.044	0.329IleTrp: 0.329 ± 0.012	0.44IleTyr: 0.44 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	2.942LysAla: 2.942 ± 0.051	0.105LysCys: 0.105 ± 0.007	1.3LysAsp: 1.3 ± 0.033	1.18LysGlu: 1.18 ± 0.032	0.458LysPhe: 0.458 ± 0.016	1.851LysGly: 1.851 ± 0.037	0.408LysHis: 0.408 ± 0.014	0.815LysIle: 0.815 ± 0.022	0.838LysLys: 0.838 ± 0.03	1.919LysLeu: 1.919 ± 0.035	0.33LysMet: 0.33 ± 0.012	0.538LysAsn: 0.538 ± 0.02	1.309LysPro: 1.309 ± 0.033	0.626LysGln: 0.626 ± 0.019	1.42LysArg: 1.42 ± 0.035	1.038LysSer: 1.038 ± 0.028	1.29LysThr: 1.29 ± 0.033	1.867LysVal: 1.867 ± 0.037	0.251LysTrp: 0.251 ± 0.011	0.412LysTyr: 0.412 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.66LeuAla: 15.66 ± 0.13	0.793LeuCys: 0.793 ± 0.023	6.996LeuAsp: 6.996 ± 0.083	4.686LeuGlu: 4.686 ± 0.066	2.654LeuPhe: 2.654 ± 0.045	9.62LeuGly: 9.62 ± 0.08	2.299LeuHis: 2.299 ± 0.036	2.86LeuIle: 2.86 ± 0.046	1.98LeuLys: 1.98 ± 0.04	11.748LeuLeu: 11.748 ± 0.116	1.526LeuMet: 1.526 ± 0.03	1.624LeuAsn: 1.624 ± 0.032	6.756LeuPro: 6.756 ± 0.071	1.989LeuGln: 1.989 ± 0.036	8.896LeuArg: 8.896 ± 0.077	5.098LeuSer: 5.098 ± 0.054	6.831LeuThr: 6.831 ± 0.074	9.361LeuVal: 9.361 ± 0.088	1.333LeuTrp: 1.333 ± 0.029	1.884LeuTyr: 1.884 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.046MetAla: 2.046 ± 0.037	0.126MetCys: 0.126 ± 0.009	0.836MetAsp: 0.836 ± 0.023	0.746MetGlu: 0.746 ± 0.021	0.421MetPhe: 0.421 ± 0.013	1.256MetGly: 1.256 ± 0.026	0.298MetHis: 0.298 ± 0.012	0.593MetIle: 0.593 ± 0.018	0.378MetLys: 0.378 ± 0.014	1.596MetLeu: 1.596 ± 0.032	0.262MetMet: 0.262 ± 0.013	0.388MetAsn: 0.388 ± 0.014	1.01MetPro: 1.01 ± 0.023	0.385MetGln: 0.385 ± 0.015	1.396MetArg: 1.396 ± 0.028	1.155MetSer: 1.155 ± 0.024	1.375MetThr: 1.375 ± 0.028	1.211MetVal: 1.211 ± 0.023	0.189MetTrp: 0.189 ± 0.011	0.292MetTyr: 0.292 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.166AsnAla: 2.166 ± 0.036	0.16AsnCys: 0.16 ± 0.01	0.904AsnAsp: 0.904 ± 0.023	0.782AsnGlu: 0.782 ± 0.02	0.428AsnPhe: 0.428 ± 0.015	1.89AsnGly: 1.89 ± 0.037	0.383AsnHis: 0.383 ± 0.014	0.55AsnIle: 0.55 ± 0.018	0.399AsnLys: 0.399 ± 0.016	1.542AsnLeu: 1.542 ± 0.033	0.266AsnMet: 0.266 ± 0.012	0.361AsnAsn: 0.361 ± 0.018	1.285AsnPro: 1.285 ± 0.026	0.462AsnGln: 0.462 ± 0.014	1.243AsnArg: 1.243 ± 0.029	0.775AsnSer: 0.775 ± 0.02	1.066AsnThr: 1.066 ± 0.028	1.337AsnVal: 1.337 ± 0.028	0.248AsnTrp: 0.248 ± 0.012	0.356AsnTyr: 0.356 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.485ProAla: 9.485 ± 0.093	0.329ProCys: 0.329 ± 0.015	4.575ProAsp: 4.575 ± 0.073	4.949ProGlu: 4.949 ± 0.058	1.574ProPhe: 1.574 ± 0.03	7.272ProGly: 7.272 ± 0.089	1.382ProHis: 1.382 ± 0.029	1.117ProIle: 1.117 ± 0.024	1.168ProLys: 1.168 ± 0.026	5.573ProLeu: 5.573 ± 0.075	0.932ProMet: 0.932 ± 0.019	0.828ProAsn: 0.828 ± 0.021	3.785ProPro: 3.785 ± 0.078	1.467ProGln: 1.467 ± 0.031	4.212ProArg: 4.212 ± 0.053	3.139ProSer: 3.139 ± 0.048	3.084ProThr: 3.084 ± 0.045	6.058ProVal: 6.058 ± 0.061	0.904ProTrp: 0.904 ± 0.022	1.478ProTyr: 1.478 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.178GlnAla: 3.178 ± 0.046	0.144GlnCys: 0.144 ± 0.009	1.237GlnAsp: 1.237 ± 0.027	1.354GlnGlu: 1.354 ± 0.027	0.593GlnPhe: 0.593 ± 0.019	2.024GlnGly: 2.024 ± 0.036	0.549GlnHis: 0.549 ± 0.016	0.968GlnIle: 0.968 ± 0.027	0.61GlnLys: 0.61 ± 0.021	2.628GlnLeu: 2.628 ± 0.039	0.395GlnMet: 0.395 ± 0.016	0.469GlnAsn: 0.469 ± 0.017	1.372GlnPro: 1.372 ± 0.038	1.019GlnGln: 1.019 ± 0.03	2.056GlnArg: 2.056 ± 0.036	1.034GlnSer: 1.034 ± 0.021	1.193GlnThr: 1.193 ± 0.026	2.076GlnVal: 2.076 ± 0.038	0.383GlnTrp: 0.383 ± 0.013	0.57GlnTyr: 0.57 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	10.7ArgAla: 10.7 ± 0.08	0.589ArgCys: 0.589 ± 0.017	4.418ArgAsp: 4.418 ± 0.056	5.136ArgGlu: 5.136 ± 0.063	2.404ArgPhe: 2.404 ± 0.036	6.193ArgGly: 6.193 ± 0.06	2.164ArgHis: 2.164 ± 0.032	3.341ArgIle: 3.341 ± 0.041	1.657ArgLys: 1.657 ± 0.034	9.128ArgLeu: 9.128 ± 0.09	1.672ArgMet: 1.672 ± 0.033	1.374ArgAsn: 1.374 ± 0.029	5.335ArgPro: 5.335 ± 0.069	2.028ArgGln: 2.028 ± 0.028	7.967ArgArg: 7.967 ± 0.075	3.998ArgSer: 3.998 ± 0.048	5.768ArgThr: 5.768 ± 0.065	6.122ArgVal: 6.122 ± 0.058	1.352ArgTrp: 1.352 ± 0.029	1.768ArgTyr: 1.768 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.326SerAla: 6.326 ± 0.057	0.371SerCys: 0.371 ± 0.015	2.469SerAsp: 2.469 ± 0.034	2.287SerGlu: 2.287 ± 0.035	1.528SerPhe: 1.528 ± 0.033	5.742SerGly: 5.742 ± 0.065	0.93SerHis: 0.93 ± 0.022	1.231SerIle: 1.231 ± 0.029	0.949SerLys: 0.949 ± 0.029	4.738SerLeu: 4.738 ± 0.05	0.971SerMet: 0.971 ± 0.023	0.761SerAsn: 0.761 ± 0.022	3.054SerPro: 3.054 ± 0.044	1.019SerGln: 1.019 ± 0.024	3.537SerArg: 3.537 ± 0.049	2.611SerSer: 2.611 ± 0.041	2.847SerThr: 2.847 ± 0.042	4.173SerVal: 4.173 ± 0.056	0.878SerTrp: 0.878 ± 0.024	1.16SerTyr: 1.16 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	9.323ThrAla: 9.323 ± 0.085	0.398ThrCys: 0.398 ± 0.015	3.576ThrAsp: 3.576 ± 0.049	3.28ThrGlu: 3.28 ± 0.042	1.537ThrPhe: 1.537 ± 0.029	7.298ThrGly: 7.298 ± 0.073	1.166ThrHis: 1.166 ± 0.023	1.491ThrIle: 1.491 ± 0.034	1.041ThrLys: 1.041 ± 0.027	5.657ThrLeu: 5.657 ± 0.057	0.82ThrMet: 0.82 ± 0.021	0.903ThrAsn: 0.903 ± 0.022	4.28ThrPro: 4.28 ± 0.062	1.115ThrGln: 1.115 ± 0.026	3.948ThrArg: 3.948 ± 0.046	2.91ThrSer: 2.91 ± 0.039	3.953ThrThr: 3.953 ± 0.06	6.27ThrVal: 6.27 ± 0.068	0.864ThrTrp: 0.864 ± 0.021	1.264ThrTyr: 1.264 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.901ValAla: 10.901 ± 0.087	0.725ValCys: 0.725 ± 0.02	5.144ValAsp: 5.144 ± 0.055	5.14ValGlu: 5.14 ± 0.051	2.473ValPhe: 2.473 ± 0.043	6.555ValGly: 6.555 ± 0.071	2.019ValHis: 2.019 ± 0.03	2.432ValIle: 2.432 ± 0.041	1.773ValLys: 1.773 ± 0.034	10.033ValLeu: 10.033 ± 0.086	1.349ValMet: 1.349 ± 0.029	1.59ValAsn: 1.59 ± 0.032	5.869ValPro: 5.869 ± 0.066	1.894ValGln: 1.894 ± 0.036	7.758ValArg: 7.758 ± 0.066	4.175ValSer: 4.175 ± 0.056	5.786ValThr: 5.786 ± 0.068	8.355ValVal: 8.355 ± 0.091	1.14ValTrp: 1.14 ± 0.024	1.643ValTyr: 1.643 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.637TrpAla: 1.637 ± 0.031	0.143TrpCys: 0.143 ± 0.009	0.803TrpAsp: 0.803 ± 0.021	0.772TrpGlu: 0.772 ± 0.019	0.505TrpPhe: 0.505 ± 0.018	1.043TrpGly: 1.043 ± 0.027	0.352TrpHis: 0.352 ± 0.014	0.579TrpIle: 0.579 ± 0.02	0.367TrpLys: 0.367 ± 0.016	1.699TrpLeu: 1.699 ± 0.03	0.261TrpMet: 0.261 ± 0.013	0.42TrpAsn: 0.42 ± 0.016	0.756TrpPro: 0.756 ± 0.02	0.555TrpGln: 0.555 ± 0.016	1.34TrpArg: 1.34 ± 0.029	0.918TrpSer: 0.918 ± 0.024	1.129TrpThr: 1.129 ± 0.025	0.918TrpVal: 0.918 ± 0.022	0.34TrpTrp: 0.34 ± 0.013	0.341TrpTyr: 0.341 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.866TyrAla: 2.866 ± 0.043	0.165TyrCys: 0.165 ± 0.01	1.465TyrAsp: 1.465 ± 0.033	1.356TyrGlu: 1.356 ± 0.031	0.581TyrPhe: 0.581 ± 0.021	2.382TyrGly: 2.382 ± 0.036	0.366TyrHis: 0.366 ± 0.014	0.381TyrIle: 0.381 ± 0.016	0.39TyrLys: 0.39 ± 0.016	2.014TyrLeu: 2.014 ± 0.036	0.219TyrMet: 0.219 ± 0.01	0.377TyrAsn: 0.377 ± 0.015	1.001TyrPro: 1.001 ± 0.027	0.525TyrGln: 0.525 ± 0.019	1.801TyrArg: 1.801 ± 0.034	0.845TyrSer: 0.845 ± 0.025	1.162TyrThr: 1.162 ± 0.028	1.674TyrVal: 1.674 ± 0.032	0.329TyrTrp: 0.329 ± 0.013	0.417TyrTyr: 0.417 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6101 proteins (1955372 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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