Gordonia phage ShayRa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Soupsvirus; unclassified Soupsvirus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XRZ6|A0A249XRZ6_9CAUD Uncharacterized protein OS=Gordonia phage ShayRa OX=2027889 GN=SEA_SHAYRA_88 PE=4 SV=1
MM1 pKa = 7.44AFSYY5 pKa = 10.65TDD7 pKa = 3.28EE8 pKa = 6.22DD9 pKa = 4.16GDD11 pKa = 4.29LIEE14 pKa = 4.52VARR17 pKa = 11.84SSQHH21 pKa = 4.6NTLLTSVVDD30 pKa = 3.91PDD32 pKa = 4.39GNSHH36 pKa = 6.08SVYY39 pKa = 10.29IPFSEE44 pKa = 3.98IPRR47 pKa = 11.84LIEE50 pKa = 4.78DD51 pKa = 3.84LAGYY55 pKa = 10.09LGG57 pKa = 3.71

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XRT6|A0A249XRT6_9CAUD Scaffolding protein OS=Gordonia phage ShayRa OX=2027889 GN=SEA_SHAYRA_18 PE=4 SV=1
MM1 pKa = 7.26SWAGSTRR8 pKa = 11.84RR9 pKa = 11.84QRR11 pKa = 11.84LPEE14 pKa = 4.27DD15 pKa = 3.05WEE17 pKa = 4.28LNYY20 pKa = 10.43RR21 pKa = 11.84LPVLSRR27 pKa = 11.84DD28 pKa = 3.11RR29 pKa = 11.84WLCQINGPGCKK40 pKa = 9.07RR41 pKa = 11.84AATDD45 pKa = 2.86VDD47 pKa = 4.22HH48 pKa = 7.59IKK50 pKa = 10.86RR51 pKa = 11.84GDD53 pKa = 3.28NHH55 pKa = 6.82DD56 pKa = 3.9RR57 pKa = 11.84SNLHH61 pKa = 6.15AVCSVCHH68 pKa = 5.87NKK70 pKa = 9.89KK71 pKa = 10.49SSAEE75 pKa = 3.97GNQAKK80 pKa = 10.41AKK82 pKa = 10.42LKK84 pKa = 9.98AQRR87 pKa = 11.84KK88 pKa = 8.52RR89 pKa = 11.84PPEE92 pKa = 3.71RR93 pKa = 11.84HH94 pKa = 5.7PGRR97 pKa = 11.84RR98 pKa = 3.38

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

15771

32

728

162.6

17.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.226 ± 0.284

0.907 ± 0.14

6.651 ± 0.209

6.867 ± 0.307

3.456 ± 0.186

8.376 ± 0.456

2.029 ± 0.155

5.041 ± 0.191

4.61 ± 0.253

7.97 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.122

3.449 ± 0.168

5.269 ± 0.185

3.259 ± 0.169

5.916 ± 0.218

5.91 ± 0.252

6.62 ± 0.218

6.873 ± 0.211

1.959 ± 0.109

3.158 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski