Amino acid dipepetide frequency for Gordonia phage ShayRa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.209AlaAla: 10.209 ± 1.104
0.761AlaCys: 0.761 ± 0.198
5.517AlaAsp: 5.517 ± 0.647
6.785AlaGlu: 6.785 ± 0.694
3.932AlaPhe: 3.932 ± 0.601
7.926AlaGly: 7.926 ± 0.897
1.458AlaHis: 1.458 ± 0.283
4.312AlaIle: 4.312 ± 0.424
4.439AlaLys: 4.439 ± 0.611
6.531AlaLeu: 6.531 ± 0.688
2.473AlaMet: 2.473 ± 0.309
2.029AlaAsn: 2.029 ± 0.381
4.883AlaPro: 4.883 ± 0.645
3.551AlaGln: 3.551 ± 0.504
5.453AlaArg: 5.453 ± 0.667
5.263AlaSer: 5.263 ± 0.559
5.897AlaThr: 5.897 ± 0.709
6.848AlaVal: 6.848 ± 0.615
1.966AlaTrp: 1.966 ± 0.35
2.029AlaTyr: 2.029 ± 0.342
0.0AlaXaa: 0.0 ± 0.0
Cys
0.698CysAla: 0.698 ± 0.191
0.19CysCys: 0.19 ± 0.128
0.761CysAsp: 0.761 ± 0.186
0.254CysGlu: 0.254 ± 0.123
0.317CysPhe: 0.317 ± 0.148
0.888CysGly: 0.888 ± 0.215
0.38CysHis: 0.38 ± 0.158
0.254CysIle: 0.254 ± 0.139
0.571CysLys: 0.571 ± 0.172
0.698CysLeu: 0.698 ± 0.24
0.254CysMet: 0.254 ± 0.132
0.507CysAsn: 0.507 ± 0.178
0.634CysPro: 0.634 ± 0.234
0.254CysGln: 0.254 ± 0.161
0.444CysArg: 0.444 ± 0.186
0.761CysSer: 0.761 ± 0.228
0.254CysThr: 0.254 ± 0.118
0.507CysVal: 0.507 ± 0.188
0.19CysTrp: 0.19 ± 0.109
0.254CysTyr: 0.254 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
5.263AspAla: 5.263 ± 0.538
0.571AspCys: 0.571 ± 0.215
5.39AspAsp: 5.39 ± 0.76
4.122AspGlu: 4.122 ± 0.59
2.346AspPhe: 2.346 ± 0.34
5.961AspGly: 5.961 ± 0.697
1.649AspHis: 1.649 ± 0.449
2.854AspIle: 2.854 ± 0.417
2.283AspLys: 2.283 ± 0.424
6.341AspLeu: 6.341 ± 0.572
1.078AspMet: 1.078 ± 0.234
2.219AspAsn: 2.219 ± 0.373
5.2AspPro: 5.2 ± 0.673
2.283AspGln: 2.283 ± 0.361
3.297AspArg: 3.297 ± 0.477
3.424AspSer: 3.424 ± 0.418
3.995AspThr: 3.995 ± 0.448
3.932AspVal: 3.932 ± 0.477
1.458AspTrp: 1.458 ± 0.315
2.854AspTyr: 2.854 ± 0.441
0.0AspXaa: 0.0 ± 0.0
Glu
8.37GluAla: 8.37 ± 0.692
0.127GluCys: 0.127 ± 0.097
4.946GluAsp: 4.946 ± 0.566
3.995GluGlu: 3.995 ± 0.663
2.41GluPhe: 2.41 ± 0.304
5.2GluGly: 5.2 ± 0.51
1.776GluHis: 1.776 ± 0.442
4.185GluIle: 4.185 ± 0.462
2.917GluLys: 2.917 ± 0.485
6.531GluLeu: 6.531 ± 0.607
1.649GluMet: 1.649 ± 0.37
1.902GluAsn: 1.902 ± 0.318
2.346GluPro: 2.346 ± 0.408
2.093GluGln: 2.093 ± 0.38
4.122GluArg: 4.122 ± 0.503
3.044GluSer: 3.044 ± 0.46
4.312GluThr: 4.312 ± 0.554
5.2GluVal: 5.2 ± 0.641
1.585GluTrp: 1.585 ± 0.315
1.966GluTyr: 1.966 ± 0.368
0.0GluXaa: 0.0 ± 0.0
Phe
3.361PheAla: 3.361 ± 0.503
0.254PheCys: 0.254 ± 0.137
3.044PheAsp: 3.044 ± 0.471
3.107PheGlu: 3.107 ± 0.453
1.015PhePhe: 1.015 ± 0.257
3.361PheGly: 3.361 ± 0.415
0.951PheHis: 0.951 ± 0.241
1.205PheIle: 1.205 ± 0.262
1.458PheLys: 1.458 ± 0.252
2.029PheLeu: 2.029 ± 0.356
0.951PheMet: 0.951 ± 0.203
1.649PheAsn: 1.649 ± 0.244
1.839PhePro: 1.839 ± 0.347
0.824PheGln: 0.824 ± 0.239
1.395PheArg: 1.395 ± 0.296
2.473PheSer: 2.473 ± 0.453
2.156PheThr: 2.156 ± 0.368
2.41PheVal: 2.41 ± 0.417
0.317PheTrp: 0.317 ± 0.128
0.761PheTyr: 0.761 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
6.405GlyAla: 6.405 ± 0.66
0.951GlyCys: 0.951 ± 0.24
5.77GlyAsp: 5.77 ± 0.602
5.644GlyGlu: 5.644 ± 0.579
2.917GlyPhe: 2.917 ± 0.577
9.068GlyGly: 9.068 ± 2.015
2.283GlyHis: 2.283 ± 0.363
3.995GlyIle: 3.995 ± 0.534
3.868GlyLys: 3.868 ± 0.533
6.468GlyLeu: 6.468 ± 0.791
1.712GlyMet: 1.712 ± 0.3
2.917GlyAsn: 2.917 ± 0.367
3.551GlyPro: 3.551 ± 0.455
2.283GlyGln: 2.283 ± 0.406
4.249GlyArg: 4.249 ± 0.501
5.39GlySer: 5.39 ± 0.706
6.024GlyThr: 6.024 ± 0.652
5.073GlyVal: 5.073 ± 0.7
2.029GlyTrp: 2.029 ± 0.286
3.107GlyTyr: 3.107 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
1.966HisAla: 1.966 ± 0.372
0.254HisCys: 0.254 ± 0.118
1.205HisAsp: 1.205 ± 0.238
1.522HisGlu: 1.522 ± 0.367
0.761HisPhe: 0.761 ± 0.221
1.585HisGly: 1.585 ± 0.328
0.38HisHis: 0.38 ± 0.157
1.141HisIle: 1.141 ± 0.263
0.571HisLys: 0.571 ± 0.164
1.966HisLeu: 1.966 ± 0.391
0.254HisMet: 0.254 ± 0.131
0.951HisAsn: 0.951 ± 0.283
0.888HisPro: 0.888 ± 0.218
0.634HisGln: 0.634 ± 0.194
1.902HisArg: 1.902 ± 0.317
0.951HisSer: 0.951 ± 0.261
1.015HisThr: 1.015 ± 0.233
1.141HisVal: 1.141 ± 0.235
0.254HisTrp: 0.254 ± 0.144
0.951HisTyr: 0.951 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
4.185IleAla: 4.185 ± 0.512
0.634IleCys: 0.634 ± 0.192
3.741IleAsp: 3.741 ± 0.487
4.692IleGlu: 4.692 ± 0.584
1.585IlePhe: 1.585 ± 0.259
3.678IleGly: 3.678 ± 0.534
1.205IleHis: 1.205 ± 0.267
2.473IleIle: 2.473 ± 0.423
1.585IleLys: 1.585 ± 0.313
3.678IleLeu: 3.678 ± 0.431
0.824IleMet: 0.824 ± 0.205
2.093IleAsn: 2.093 ± 0.418
3.361IlePro: 3.361 ± 0.391
1.395IleGln: 1.395 ± 0.295
3.107IleArg: 3.107 ± 0.373
3.234IleSer: 3.234 ± 0.356
3.614IleThr: 3.614 ± 0.377
3.488IleVal: 3.488 ± 0.461
0.698IleTrp: 0.698 ± 0.174
1.141IleTyr: 1.141 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.263LysAla: 5.263 ± 0.652
0.254LysCys: 0.254 ± 0.142
2.346LysAsp: 2.346 ± 0.314
3.107LysGlu: 3.107 ± 0.444
1.268LysPhe: 1.268 ± 0.294
3.297LysGly: 3.297 ± 0.337
0.571LysHis: 0.571 ± 0.183
2.283LysIle: 2.283 ± 0.431
3.234LysLys: 3.234 ± 0.589
4.692LysLeu: 4.692 ± 0.44
0.888LysMet: 0.888 ± 0.226
1.141LysAsn: 1.141 ± 0.224
2.98LysPro: 2.98 ± 0.485
1.966LysGln: 1.966 ± 0.287
2.727LysArg: 2.727 ± 0.459
2.219LysSer: 2.219 ± 0.381
2.156LysThr: 2.156 ± 0.407
3.805LysVal: 3.805 ± 0.519
0.824LysTrp: 0.824 ± 0.234
1.078LysTyr: 1.078 ± 0.292
0.0LysXaa: 0.0 ± 0.0
Leu
7.546LeuAla: 7.546 ± 0.666
0.634LeuCys: 0.634 ± 0.192
5.517LeuAsp: 5.517 ± 0.645
6.785LeuGlu: 6.785 ± 0.514
2.473LeuPhe: 2.473 ± 0.408
6.722LeuGly: 6.722 ± 0.702
1.649LeuHis: 1.649 ± 0.396
4.312LeuIle: 4.312 ± 0.537
2.79LeuLys: 2.79 ± 0.566
5.453LeuLeu: 5.453 ± 0.655
2.41LeuMet: 2.41 ± 0.41
2.473LeuAsn: 2.473 ± 0.38
4.312LeuPro: 4.312 ± 0.524
1.902LeuGln: 1.902 ± 0.327
4.883LeuArg: 4.883 ± 0.459
4.946LeuSer: 4.946 ± 0.585
4.566LeuThr: 4.566 ± 0.596
6.151LeuVal: 6.151 ± 0.535
1.712LeuTrp: 1.712 ± 0.281
2.473LeuTyr: 2.473 ± 0.411
0.0LeuXaa: 0.0 ± 0.0
Met
2.473MetAla: 2.473 ± 0.315
0.19MetCys: 0.19 ± 0.127
0.951MetAsp: 0.951 ± 0.235
1.395MetGlu: 1.395 ± 0.245
1.205MetPhe: 1.205 ± 0.292
1.458MetGly: 1.458 ± 0.27
0.317MetHis: 0.317 ± 0.18
1.268MetIle: 1.268 ± 0.347
1.141MetLys: 1.141 ± 0.254
1.649MetLeu: 1.649 ± 0.323
0.698MetMet: 0.698 ± 0.227
0.698MetAsn: 0.698 ± 0.243
1.458MetPro: 1.458 ± 0.335
0.698MetGln: 0.698 ± 0.23
1.839MetArg: 1.839 ± 0.33
2.219MetSer: 2.219 ± 0.36
2.854MetThr: 2.854 ± 0.366
1.205MetVal: 1.205 ± 0.27
0.127MetTrp: 0.127 ± 0.092
0.698MetTyr: 0.698 ± 0.226
0.0MetXaa: 0.0 ± 0.0
Asn
3.234AsnAla: 3.234 ± 0.563
0.571AsnCys: 0.571 ± 0.238
1.902AsnAsp: 1.902 ± 0.291
1.776AsnGlu: 1.776 ± 0.352
0.951AsnPhe: 0.951 ± 0.263
3.805AsnGly: 3.805 ± 0.602
0.571AsnHis: 0.571 ± 0.183
1.458AsnIle: 1.458 ± 0.349
1.585AsnLys: 1.585 ± 0.36
2.98AsnLeu: 2.98 ± 0.476
0.824AsnMet: 0.824 ± 0.239
0.824AsnAsn: 0.824 ± 0.241
2.473AsnPro: 2.473 ± 0.349
1.458AsnGln: 1.458 ± 0.257
1.839AsnArg: 1.839 ± 0.341
2.029AsnSer: 2.029 ± 0.297
2.663AsnThr: 2.663 ± 0.354
1.585AsnVal: 1.585 ± 0.295
0.634AsnTrp: 0.634 ± 0.167
1.332AsnTyr: 1.332 ± 0.275
0.0AsnXaa: 0.0 ± 0.0
Pro
4.756ProAla: 4.756 ± 0.558
0.38ProCys: 0.38 ± 0.182
3.868ProAsp: 3.868 ± 0.521
4.185ProGlu: 4.185 ± 0.457
1.902ProPhe: 1.902 ± 0.291
4.883ProGly: 4.883 ± 0.542
0.824ProHis: 0.824 ± 0.214
2.98ProIle: 2.98 ± 0.399
3.171ProLys: 3.171 ± 0.449
2.536ProLeu: 2.536 ± 0.457
1.395ProMet: 1.395 ± 0.289
2.093ProAsn: 2.093 ± 0.395
2.156ProPro: 2.156 ± 0.396
1.585ProGln: 1.585 ± 0.316
2.917ProArg: 2.917 ± 0.455
2.6ProSer: 2.6 ± 0.356
4.375ProThr: 4.375 ± 0.428
3.614ProVal: 3.614 ± 0.421
0.761ProTrp: 0.761 ± 0.236
1.712ProTyr: 1.712 ± 0.356
0.0ProXaa: 0.0 ± 0.0
Gln
3.297GlnAla: 3.297 ± 0.444
0.317GlnCys: 0.317 ± 0.154
1.522GlnAsp: 1.522 ± 0.369
2.219GlnGlu: 2.219 ± 0.394
1.268GlnPhe: 1.268 ± 0.254
2.029GlnGly: 2.029 ± 0.302
0.507GlnHis: 0.507 ± 0.158
1.839GlnIle: 1.839 ± 0.283
1.141GlnLys: 1.141 ± 0.3
3.741GlnLeu: 3.741 ± 0.493
1.015GlnMet: 1.015 ± 0.252
1.268GlnAsn: 1.268 ± 0.356
1.141GlnPro: 1.141 ± 0.325
0.951GlnGln: 0.951 ± 0.23
2.156GlnArg: 2.156 ± 0.368
1.776GlnSer: 1.776 ± 0.326
1.902GlnThr: 1.902 ± 0.355
2.41GlnVal: 2.41 ± 0.436
0.761GlnTrp: 0.761 ± 0.192
1.332GlnTyr: 1.332 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
4.692ArgAla: 4.692 ± 0.567
0.698ArgCys: 0.698 ± 0.309
3.488ArgAsp: 3.488 ± 0.38
3.995ArgGlu: 3.995 ± 0.65
2.156ArgPhe: 2.156 ± 0.382
3.995ArgGly: 3.995 ± 0.443
1.522ArgHis: 1.522 ± 0.315
3.932ArgIle: 3.932 ± 0.504
2.98ArgLys: 2.98 ± 0.437
4.756ArgLeu: 4.756 ± 0.661
1.902ArgMet: 1.902 ± 0.308
2.219ArgAsn: 2.219 ± 0.332
2.156ArgPro: 2.156 ± 0.353
1.585ArgGln: 1.585 ± 0.289
5.01ArgArg: 5.01 ± 0.669
3.551ArgSer: 3.551 ± 0.466
3.107ArgThr: 3.107 ± 0.475
4.058ArgVal: 4.058 ± 0.397
1.078ArgTrp: 1.078 ± 0.246
2.283ArgTyr: 2.283 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
5.327SerAla: 5.327 ± 0.598
0.254SerCys: 0.254 ± 0.113
3.107SerAsp: 3.107 ± 0.543
3.361SerGlu: 3.361 ± 0.41
2.156SerPhe: 2.156 ± 0.339
5.834SerGly: 5.834 ± 0.735
0.824SerHis: 0.824 ± 0.209
2.854SerIle: 2.854 ± 0.476
2.663SerLys: 2.663 ± 0.426
5.453SerLeu: 5.453 ± 0.644
1.902SerMet: 1.902 ± 0.306
2.79SerAsn: 2.79 ± 0.568
2.41SerPro: 2.41 ± 0.34
2.346SerGln: 2.346 ± 0.369
3.361SerArg: 3.361 ± 0.409
3.488SerSer: 3.488 ± 0.519
3.995SerThr: 3.995 ± 0.594
4.122SerVal: 4.122 ± 0.483
1.332SerTrp: 1.332 ± 0.286
1.522SerTyr: 1.522 ± 0.246
0.0SerXaa: 0.0 ± 0.0
Thr
5.77ThrAla: 5.77 ± 0.637
0.888ThrCys: 0.888 ± 0.252
4.375ThrAsp: 4.375 ± 0.489
3.868ThrGlu: 3.868 ± 0.547
2.029ThrPhe: 2.029 ± 0.428
5.517ThrGly: 5.517 ± 0.59
1.015ThrHis: 1.015 ± 0.244
3.424ThrIle: 3.424 ± 0.44
3.995ThrLys: 3.995 ± 0.657
5.2ThrLeu: 5.2 ± 0.553
1.458ThrMet: 1.458 ± 0.349
1.839ThrAsn: 1.839 ± 0.341
4.692ThrPro: 4.692 ± 0.493
2.283ThrGln: 2.283 ± 0.409
2.6ThrArg: 2.6 ± 0.357
3.932ThrSer: 3.932 ± 0.539
3.805ThrThr: 3.805 ± 0.509
5.263ThrVal: 5.263 ± 0.559
1.649ThrTrp: 1.649 ± 0.278
2.6ThrTyr: 2.6 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
5.453ValAla: 5.453 ± 0.579
0.761ValCys: 0.761 ± 0.244
5.263ValAsp: 5.263 ± 0.559
4.439ValGlu: 4.439 ± 0.434
1.902ValPhe: 1.902 ± 0.399
4.692ValGly: 4.692 ± 0.475
1.268ValHis: 1.268 ± 0.247
3.488ValIle: 3.488 ± 0.581
3.678ValLys: 3.678 ± 0.45
5.263ValLeu: 5.263 ± 0.671
1.141ValMet: 1.141 ± 0.241
2.663ValAsn: 2.663 ± 0.374
4.185ValPro: 4.185 ± 0.57
2.346ValGln: 2.346 ± 0.388
4.629ValArg: 4.629 ± 0.548
4.439ValSer: 4.439 ± 0.495
5.263ValThr: 5.263 ± 0.612
4.502ValVal: 4.502 ± 0.496
1.205ValTrp: 1.205 ± 0.248
2.156ValTyr: 2.156 ± 0.381
0.0ValXaa: 0.0 ± 0.0
Trp
1.649TrpAla: 1.649 ± 0.383
0.317TrpCys: 0.317 ± 0.15
1.522TrpAsp: 1.522 ± 0.315
1.585TrpGlu: 1.585 ± 0.307
0.824TrpPhe: 0.824 ± 0.201
1.205TrpGly: 1.205 ± 0.297
0.444TrpHis: 0.444 ± 0.165
0.951TrpIle: 0.951 ± 0.215
0.634TrpLys: 0.634 ± 0.178
0.888TrpLeu: 0.888 ± 0.19
0.38TrpMet: 0.38 ± 0.137
1.015TrpAsn: 1.015 ± 0.294
0.698TrpPro: 0.698 ± 0.226
1.015TrpGln: 1.015 ± 0.241
1.078TrpArg: 1.078 ± 0.218
1.395TrpSer: 1.395 ± 0.266
1.522TrpThr: 1.522 ± 0.27
1.332TrpVal: 1.332 ± 0.261
0.634TrpTrp: 0.634 ± 0.241
0.507TrpTyr: 0.507 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.346TyrAla: 2.346 ± 0.454
0.063TyrCys: 0.063 ± 0.066
2.283TyrAsp: 2.283 ± 0.355
1.839TyrGlu: 1.839 ± 0.314
1.141TyrPhe: 1.141 ± 0.361
2.663TyrGly: 2.663 ± 0.346
0.698TyrHis: 0.698 ± 0.201
1.205TyrIle: 1.205 ± 0.285
1.395TyrLys: 1.395 ± 0.295
2.854TyrLeu: 2.854 ± 0.477
1.268TyrMet: 1.268 ± 0.279
1.205TyrAsn: 1.205 ± 0.285
1.332TyrPro: 1.332 ± 0.248
1.141TyrGln: 1.141 ± 0.238
2.156TyrArg: 2.156 ± 0.337
1.966TyrSer: 1.966 ± 0.444
2.727TyrThr: 2.727 ± 0.4
2.093TyrVal: 2.093 ± 0.36
0.38TyrTrp: 0.38 ± 0.165
0.824TyrTyr: 0.824 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (15771 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski