Apis mellifera associated microvirus 55
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S8UTM4|A0A3S8UTM4_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 55 OX=2494786 PE=4 SV=1
MM1 pKa = 7.14 LHH3 pKa = 6.84 CRR5 pKa = 11.84 TINYY9 pKa = 9.44 SCTGKK14 pKa = 9.75 NRR16 pKa = 11.84 QYY18 pKa = 11.78 KK19 pKa = 8.81 MKK21 pKa = 10.61 QSGNTYY27 pKa = 10.35 YY28 pKa = 10.15 LTDD31 pKa = 3.97 EE32 pKa = 5.17 PDD34 pKa = 3.41 PHH36 pKa = 7.59 DD37 pKa = 3.91 MPSYY41 pKa = 11.01 EE42 pKa = 4.37 DD43 pKa = 3.65 TLLQYY48 pKa = 10.78 ADD50 pKa = 3.9 PMFASGFVDD59 pKa = 4.51 PSSLLASSAHH69 pKa = 6.21 AEE71 pKa = 4.19 SAAEE75 pKa = 4.26 AIIHH79 pKa = 5.6 SVEE82 pKa = 3.91 SSNNIEE88 pKa = 4.1 NEE90 pKa = 3.69 II91 pKa = 4.01
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.476
IPC2_protein 4.647
IPC_protein 4.507
Toseland 4.38
ProMoST 4.635
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.355
Rodwell 4.368
Grimsley 4.291
Solomon 4.469
Lehninger 4.418
Nozaki 4.596
DTASelect 4.749
Thurlkill 4.393
EMBOSS 4.38
Sillero 4.647
Patrickios 0.693
IPC_peptide 4.469
IPC2_peptide 4.635
IPC2.peptide.svr19 4.547
Protein with the highest isoelectric point:
>tr|A0A3S8UTN5|A0A3S8UTN5_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 55 OX=2494786 PE=4 SV=1
MM1 pKa = 7.72 CSMLHH6 pKa = 6.05 YY7 pKa = 9.02 LTHH10 pKa = 6.18 PQSNAVVHH18 pKa = 6.28 CPLYY22 pKa = 9.97 TGVLRR27 pKa = 11.84 GLPLRR32 pKa = 11.84 QLVLSSRR39 pKa = 11.84 TVVSCQWIKK48 pKa = 11.08 RR49 pKa = 11.84 DD50 pKa = 3.25 VLYY53 pKa = 10.97 NFRR56 pKa = 11.84 ACVPHH61 pKa = 6.29 ACAYY65 pKa = 9.66 ILL67 pKa = 4.27
Molecular weight: 7.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.029
IPC2_protein 8.39
IPC_protein 8.536
Toseland 8.112
ProMoST 8.726
Dawson 8.873
Bjellqvist 9.268
Wikipedia 8.829
Rodwell 8.873
Grimsley 7.863
Solomon 9.121
Lehninger 9.107
Nozaki 9.428
DTASelect 8.96
Thurlkill 8.96
EMBOSS 9.063
Sillero 9.297
Patrickios 0.744
IPC_peptide 9.107
IPC2_peptide 9.048
IPC2.peptide.svr19 8.767
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1753
67
682
350.6
39.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.275 ± 1.219
1.711 ± 0.685
6.617 ± 0.707
4.678 ± 0.944
4.906 ± 1.141
5.705 ± 0.903
2.567 ± 0.685
6.104 ± 0.496
6.218 ± 1.225
9.241 ± 0.629
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.387
5.59 ± 0.667
4.278 ± 0.704
3.708 ± 0.765
4.45 ± 0.641
8.614 ± 1.299
5.02 ± 0.781
5.476 ± 0.633
1.198 ± 0.457
5.305 ± 0.966
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here