Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces japonicus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6JXX1|B6JXX1_SCHJY RNA lariat debranching enzyme Dbr1 OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=SJAG_01433 PE=3 SV=1
MM1 pKa = 7.43SFYY4 pKa = 11.26DD5 pKa = 4.19EE6 pKa = 5.37IEE8 pKa = 4.69LEE10 pKa = 4.6DD11 pKa = 3.77FTFDD15 pKa = 3.72SVNNIYY21 pKa = 8.59TFPCPCGDD29 pKa = 3.18RR30 pKa = 11.84FEE32 pKa = 5.79ISLEE36 pKa = 3.92DD37 pKa = 3.75LKK39 pKa = 11.48NGEE42 pKa = 4.44DD43 pKa = 3.62VARR46 pKa = 11.84CPSCSLIVRR55 pKa = 11.84VIFDD59 pKa = 3.25EE60 pKa = 4.46DD61 pKa = 3.85MFVEE65 pKa = 4.51TEE67 pKa = 3.86DD68 pKa = 3.97TEE70 pKa = 4.52QVEE73 pKa = 4.45APVVVVAA80 pKa = 5.56

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6K417|B6K417_SCHJY Uncharacterized protein OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=SJAG_03365 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.54LAKK15 pKa = 9.74AQRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTINYY37 pKa = 8.41NMKK40 pKa = 9.1RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.35WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.12LNII51 pKa = 3.83

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4852

0

4852

2271437

33

4968

468.1

52.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.304 ± 0.034

1.529 ± 0.013

5.314 ± 0.025

6.521 ± 0.037

4.328 ± 0.025

5.014 ± 0.033

2.357 ± 0.015

5.232 ± 0.028

5.899 ± 0.035

9.854 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.012

4.534 ± 0.018

4.892 ± 0.032

4.109 ± 0.024

5.447 ± 0.031

8.81 ± 0.05

5.864 ± 0.04

6.516 ± 0.028

1.131 ± 0.012

3.178 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski