Liberibacter asiaticus (strain psy62)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6XHN6|C6XHN6_LIBAP DNA topoisomerase 1 OS=Liberibacter asiaticus (strain psy62) OX=537021 GN=topA PE=3 SV=1
MM1 pKa = 7.92 AGGLDD6 pKa = 3.58 CLYY9 pKa = 10.45 PPEE12 pKa = 4.16 NRR14 pKa = 11.84 NLLEE18 pKa = 4.66 EE19 pKa = 3.89 IWDD22 pKa = 3.73 NGGIAISEE30 pKa = 4.22 IPFGG34 pKa = 3.87
Molecular weight: 3.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.881
IPC2_protein 3.91
IPC_protein 3.567
Toseland 3.452
ProMoST 3.795
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.554
Rodwell 3.452
Grimsley 3.401
Solomon 3.478
Lehninger 3.439
Nozaki 3.77
DTASelect 3.783
Thurlkill 3.567
EMBOSS 3.554
Sillero 3.706
Patrickios 1.825
IPC_peptide 3.478
IPC2_peptide 3.668
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|C6XF22|C6XF22_LIBAP Uncharacterized protein OS=Liberibacter asiaticus (strain psy62) OX=537021 GN=CLIBASIA_01940 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.45 RR3 pKa = 11.84 TYY5 pKa = 10.46 NPSNIVRR12 pKa = 11.84 KK13 pKa = 9.52 RR14 pKa = 11.84 RR15 pKa = 11.84 CGFLARR21 pKa = 11.84 MSTRR25 pKa = 11.84 SGIRR29 pKa = 11.84 ILNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.34 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 10.891
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.135
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.871
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1102
0
1102
316885
30
1828
287.6
32.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.354 ± 0.066
1.392 ± 0.029
5.357 ± 0.056
5.809 ± 0.067
4.548 ± 0.064
6.191 ± 0.072
2.349 ± 0.04
8.873 ± 0.074
6.448 ± 0.067
9.576 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.704 ± 0.038
4.606 ± 0.057
3.719 ± 0.044
3.556 ± 0.049
5.26 ± 0.055
8.12 ± 0.065
4.661 ± 0.055
6.433 ± 0.073
1.002 ± 0.03
3.044 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here