Pseudomonas virus PMG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Detrevirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2BDB4|H2BDB4_9CAUD Putative head-tail adaptor OS=Pseudomonas virus PMG1 OX=1129146 GN=PMG1_00010 PE=4 SV=1
MM1 pKa = 7.59SLIPLDD7 pKa = 3.8TAKK10 pKa = 10.94SFLDD14 pKa = 5.15VIHH17 pKa = 7.24DD18 pKa = 3.59WDD20 pKa = 4.08DD21 pKa = 3.7AKK23 pKa = 11.25LQLLLDD29 pKa = 4.12GAEE32 pKa = 4.76DD33 pKa = 3.88EE34 pKa = 5.24ACQFMWRR41 pKa = 11.84QSLDD45 pKa = 3.35GLCNCEE51 pKa = 4.01EE52 pKa = 4.13SSEE55 pKa = 4.32AVSSEE60 pKa = 3.78PGLPPSVVIGVLLLLQASYY79 pKa = 10.58QAAPDD84 pKa = 4.13EE85 pKa = 4.36IATLRR90 pKa = 11.84KK91 pKa = 9.05AAEE94 pKa = 4.35VKK96 pKa = 10.42LMPYY100 pKa = 10.05RR101 pKa = 11.84CGLGVV106 pKa = 3.15

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2BDG2|H2BDG2_9CAUD Uncharacterized protein OS=Pseudomonas virus PMG1 OX=1129146 GN=PMG1_00058 PE=4 SV=1
MM1 pKa = 7.3TSSTTHH7 pKa = 6.3NATSSRR13 pKa = 11.84EE14 pKa = 4.2SASGRR19 pKa = 11.84SPFAAPAGQMIDD31 pKa = 3.23LFGPVPVLANLSPRR45 pKa = 11.84QAKK48 pKa = 9.66EE49 pKa = 3.65LGLMTRR55 pKa = 11.84VISGHH60 pKa = 5.97ILPGSSASAALEE72 pKa = 3.8KK73 pKa = 11.0SLVSRR78 pKa = 11.84LQARR82 pKa = 11.84TRR84 pKa = 11.84SLGSTLYY91 pKa = 11.07KK92 pKa = 9.82LTWKK96 pKa = 8.85PWSMPSGLLRR106 pKa = 11.84SRR108 pKa = 11.84LRR110 pKa = 11.84ASVPRR115 pKa = 11.84TSEE118 pKa = 3.59TGRR121 pKa = 11.84TGWGTPSARR130 pKa = 11.84DD131 pKa = 3.46WKK133 pKa = 10.55SASATEE139 pKa = 3.82EE140 pKa = 3.9WLAGRR145 pKa = 11.84LEE147 pKa = 4.08HH148 pKa = 7.38SRR150 pKa = 11.84GKK152 pKa = 9.62PLSEE156 pKa = 4.3QAFTLAGWPTCTANDD171 pKa = 3.47AEE173 pKa = 4.51KK174 pKa = 10.28RR175 pKa = 11.84GKK177 pKa = 9.93VSYY180 pKa = 10.67RR181 pKa = 11.84EE182 pKa = 3.9NAPNGLNAIATLSGPARR199 pKa = 11.84LTASGQMLTGSSAGMEE215 pKa = 4.14SGGQLNPAHH224 pKa = 6.44SRR226 pKa = 11.84WLMGLPPEE234 pKa = 4.61WDD236 pKa = 2.8ACAPTEE242 pKa = 4.2TPSMLKK248 pKa = 10.0RR249 pKa = 11.84RR250 pKa = 11.84RR251 pKa = 11.84SSS253 pKa = 2.86

Molecular weight: 26.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

16729

31

909

174.3

19.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.975 ± 0.482

1.739 ± 0.178

5.47 ± 0.237

6.671 ± 0.299

3.294 ± 0.194

7.604 ± 0.229

1.955 ± 0.17

5.374 ± 0.261

4.507 ± 0.268

8.584 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.154

3.294 ± 0.191

4.561 ± 0.242

3.79 ± 0.221

7.084 ± 0.286

6.504 ± 0.319

4.836 ± 0.27

6.516 ± 0.246

1.805 ± 0.135

2.887 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski