Pedobacter sp. Leaf216

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4G7J9|A0A0Q4G7J9_9SPHI Sodium:solute symporter OS=Pedobacter sp. Leaf216 OX=1735684 GN=ASE74_05085 PE=3 SV=1
MM1 pKa = 7.67INPDD5 pKa = 2.86GTYY8 pKa = 9.94TYY10 pKa = 10.56TPAPNYY16 pKa = 10.61NGTDD20 pKa = 3.31SFTVTVSDD28 pKa = 3.99GKK30 pKa = 11.28GGTTTVTINVTITPVNDD47 pKa = 3.48APVATAPAIVTNKK60 pKa = 9.91NIPVNGTITASDD72 pKa = 3.54VDD74 pKa = 4.0GDD76 pKa = 4.09ALTFTVSTPPAHH88 pKa = 5.69GTVVVKK94 pKa = 10.88ADD96 pKa = 3.27GTYY99 pKa = 10.2TYY101 pKa = 10.32TPANNYY107 pKa = 9.78SGSDD111 pKa = 3.45VFTVTVSDD119 pKa = 4.07GKK121 pKa = 11.22GGTATVTINVTINPTNVAPVVTAPAVTGNEE151 pKa = 3.95DD152 pKa = 3.45SPINGTITATDD163 pKa = 3.62ADD165 pKa = 4.47GDD167 pKa = 4.2PLTFTVATPPSNGTVVVNPDD187 pKa = 2.68GTYY190 pKa = 10.12TYY192 pKa = 10.56TPAPNYY198 pKa = 10.61NGTDD202 pKa = 3.31SFTVTVSDD210 pKa = 3.99GKK212 pKa = 11.28GGTTTVTVNVNVTPVNDD229 pKa = 3.77APVATSPAIVTNKK242 pKa = 9.94NIPVNGTITASDD254 pKa = 3.54VDD256 pKa = 4.0GDD258 pKa = 4.09ALTFTVSTPPAHH270 pKa = 5.76GTVVVNADD278 pKa = 3.0GTYY281 pKa = 10.14TYY283 pKa = 10.48TPANNYY289 pKa = 9.78SGSDD293 pKa = 3.45VFTVTVSDD301 pKa = 3.96GKK303 pKa = 11.28GGTTTVTINVTVNPTNVAPVVTAPAVTGNEE333 pKa = 3.95DD334 pKa = 3.45SPINGTITATDD345 pKa = 3.62ADD347 pKa = 4.47GDD349 pKa = 4.2PLTFTVATPPANGTVVVNPDD369 pKa = 2.68GTYY372 pKa = 10.15TYY374 pKa = 10.66TPVPNYY380 pKa = 10.71NGTDD384 pKa = 3.37SFTVTVSDD392 pKa = 3.99GKK394 pKa = 11.28GGTTTVTINVTVTPVNDD411 pKa = 3.82APVATAPAIVTNKK424 pKa = 9.91NIPVNGTITASDD436 pKa = 3.54VDD438 pKa = 4.0GDD440 pKa = 4.09ALTFTVSTPPAHH452 pKa = 5.76GTVVVNADD460 pKa = 3.0GTYY463 pKa = 10.14TYY465 pKa = 10.48TPANNYY471 pKa = 9.78SGSDD475 pKa = 3.45VFTVTVSDD483 pKa = 3.96GKK485 pKa = 11.28GGTTTVTINVTINPTNVAPVVTAPAVTGNEE515 pKa = 3.95DD516 pKa = 3.45SPINGTITATDD527 pKa = 3.62ADD529 pKa = 4.47GDD531 pKa = 4.22PLTFTVSTPPAHH543 pKa = 5.66GTVVINPDD551 pKa = 2.67GTYY554 pKa = 10.01TYY556 pKa = 10.56TPAPNYY562 pKa = 10.61NGTDD566 pKa = 3.31SFTVTVSDD574 pKa = 3.99GKK576 pKa = 11.28GGTTTVTINVTVTPPEE592 pKa = 4.59TISXTPPAHH601 pKa = 5.29GTVVINPDD609 pKa = 2.67GTYY612 pKa = 10.01TYY614 pKa = 10.56TPAPNYY620 pKa = 10.61NGTDD624 pKa = 3.31SFTVTVSDD632 pKa = 3.99GKK634 pKa = 11.28GGTTTVTINVIINPVNDD651 pKa = 3.98APVATAPAIVTNKK664 pKa = 9.91NIPVNGTITASDD676 pKa = 3.54VDD678 pKa = 4.0GDD680 pKa = 4.09ALTFTVSTPPAHH692 pKa = 6.07GTVTVNADD700 pKa = 2.98GTYY703 pKa = 10.12TYY705 pKa = 10.48TPANNYY711 pKa = 9.78SGSDD715 pKa = 3.45VFTVTVSDD723 pKa = 4.07GKK725 pKa = 11.22GGTATVTINVTINPTNVAPVVTAPAVTGNEE755 pKa = 3.95DD756 pKa = 3.45SPINGTITATDD767 pKa = 3.62ADD769 pKa = 4.47GDD771 pKa = 4.2PLTFTVATPPAHH783 pKa = 5.67GTVTVNADD791 pKa = 2.98GTYY794 pKa = 9.93TYY796 pKa = 10.65TPAPNYY802 pKa = 10.61NGTDD806 pKa = 3.31SFTVTVSDD814 pKa = 3.99GKK816 pKa = 11.28GGTTTVTVNVNVTPVNDD833 pKa = 3.77APVATSPAIVTNKK846 pKa = 9.94NIPVNGTITASDD858 pKa = 3.54VDD860 pKa = 4.0GDD862 pKa = 4.09ALTFTVSTPPAHH874 pKa = 6.07GTVTVNADD882 pKa = 2.98GTYY885 pKa = 10.12TYY887 pKa = 10.48TPANNYY893 pKa = 9.78SGSDD897 pKa = 3.45VFTVTVSDD905 pKa = 3.96GKK907 pKa = 11.28GGTTTVTINVTVNPTNVAPFVTSPAVTGNEE937 pKa = 3.88DD938 pKa = 3.46SPINSSITATDD949 pKa = 3.53ADD951 pKa = 4.4GDD953 pKa = 4.22PLTFTVSTPPAHH965 pKa = 5.83GTVVVNPDD973 pKa = 2.68GTYY976 pKa = 10.12TYY978 pKa = 10.56TPAPNYY984 pKa = 10.61NGTDD988 pKa = 3.31SFTVTVSDD996 pKa = 3.99GKK998 pKa = 11.28GGTTTVTVNVTVTPVNDD1015 pKa = 3.61APVATSPAIVTNKK1028 pKa = 9.94NIPVNGTITASDD1040 pKa = 3.54VDD1042 pKa = 4.0GDD1044 pKa = 4.09ALTFTVSTPPAHH1056 pKa = 5.91GTVTVKK1062 pKa = 10.95ADD1064 pKa = 3.24GTYY1067 pKa = 10.18TYY1069 pKa = 10.32TPANNYY1075 pKa = 9.78SGSDD1079 pKa = 3.45VFTVTVSDD1087 pKa = 3.96GKK1089 pKa = 11.28GGTTTVTINVTINPTNVAPVVTAPAVTGNEE1119 pKa = 3.95DD1120 pKa = 3.45SPINGTITATDD1131 pKa = 3.6ADD1133 pKa = 4.51GDD1135 pKa = 4.12PLIFTVATPPAHH1147 pKa = 5.67GTVTVNADD1155 pKa = 3.02GTYY1158 pKa = 10.07TYY1160 pKa = 10.69IPAPNYY1166 pKa = 10.61NGTDD1170 pKa = 3.31SFTVTVSDD1178 pKa = 3.99GKK1180 pKa = 11.28GGTTTVTVNVTVTPVNDD1197 pKa = 3.91APVTTAPAVTTPQNTAVNGAITASDD1222 pKa = 3.54VDD1224 pKa = 3.93GDD1226 pKa = 4.21ALTFTLTAQPAHH1238 pKa = 5.74GTVVVNANGTYY1249 pKa = 9.83TYY1251 pKa = 9.89TPTAGYY1257 pKa = 9.76SGNDD1261 pKa = 3.09TFTITVSDD1269 pKa = 3.94GKK1271 pKa = 10.97GGTVTVTVPVTVTLVAAPAMSLTKK1295 pKa = 9.92TATNTVSKK1303 pKa = 10.81VGDD1306 pKa = 3.61VINYY1310 pKa = 9.36NIIVTNTGNVTLTNVAVTDD1329 pKa = 4.21AGADD1333 pKa = 3.51AGSLTPAGIASLLPGSSVTVTARR1356 pKa = 11.84HH1357 pKa = 5.22TVTLTEE1363 pKa = 4.14VNAGSFSNQASATAQTPGGSILNKK1387 pKa = 9.85PKK1389 pKa = 10.6SDD1391 pKa = 4.18DD1392 pKa = 3.85PNTIAVDD1399 pKa = 3.75DD1400 pKa = 4.06ATVTVIAPASMITLVKK1416 pKa = 9.58TGTVSTDD1423 pKa = 3.12GNSITYY1429 pKa = 9.97SFSVKK1434 pKa = 8.97NTGNVTLHH1442 pKa = 7.06IITLVDD1448 pKa = 3.54AKK1450 pKa = 10.93LGLNRR1455 pKa = 11.84VIPGTLAPGATVTDD1469 pKa = 3.86SYY1471 pKa = 11.75VYY1473 pKa = 10.74QLTQADD1479 pKa = 3.97KK1480 pKa = 11.31DD1481 pKa = 3.84AGSVTNTASITGQTPANVAVSDD1503 pKa = 3.65ISGTAEE1509 pKa = 3.86NNNTPTVTLIPNNGSVALVKK1529 pKa = 10.16TSVFNGNKK1537 pKa = 6.32VTYY1540 pKa = 9.59TFTIKK1545 pKa = 9.56NTGTITLNTITLTDD1559 pKa = 3.69AKK1561 pKa = 10.69LGLNNKK1567 pKa = 9.2VITVAGGLAPGATTTDD1583 pKa = 3.41VEE1585 pKa = 5.23VYY1587 pKa = 10.12TLTQADD1593 pKa = 4.13KK1594 pKa = 11.53DD1595 pKa = 4.02LGTVTNTATVNARR1608 pKa = 11.84TLAGASVTDD1617 pKa = 3.32VSGTAEE1623 pKa = 4.27TNNTPTVTTFPKK1635 pKa = 10.65SPTAVDD1641 pKa = 4.71DD1642 pKa = 4.12KK1643 pKa = 11.36AQTVANSPVVISVLNNDD1660 pKa = 4.15DD1661 pKa = 4.74PGNSTLDD1668 pKa = 3.41KK1669 pKa = 10.54LTVEE1673 pKa = 4.55VVNAPKK1679 pKa = 10.54HH1680 pKa = 4.79GTVKK1684 pKa = 10.49VNADD1688 pKa = 3.28GTVTYY1693 pKa = 9.57TPDD1696 pKa = 3.5PGYY1699 pKa = 11.22VGDD1702 pKa = 5.2DD1703 pKa = 3.23VFTYY1707 pKa = 10.06RR1708 pKa = 11.84VKK1710 pKa = 10.74DD1711 pKa = 3.42AYY1713 pKa = 10.55GYY1715 pKa = 6.52YY1716 pKa = 10.08TNVATVTLTANFTGITVPNLFTPNGDD1742 pKa = 3.95GINDD1746 pKa = 3.63TFEE1749 pKa = 5.85IIGLNQYY1756 pKa = 8.03QVNEE1760 pKa = 3.97LQIVNRR1766 pKa = 11.84WGNEE1770 pKa = 3.45VFHH1773 pKa = 7.28AKK1775 pKa = 10.2GYY1777 pKa = 8.02QNNWTGEE1784 pKa = 3.99GLNEE1788 pKa = 3.67GTYY1791 pKa = 10.78YY1792 pKa = 11.08YY1793 pKa = 11.2LLRR1796 pKa = 11.84VKK1798 pKa = 10.45KK1799 pKa = 10.38AGSDD1803 pKa = 3.25QYY1805 pKa = 11.61EE1806 pKa = 4.23VFKK1809 pKa = 11.09GYY1811 pKa = 8.32ITLIKK1816 pKa = 10.31AFKK1819 pKa = 10.15KK1820 pKa = 10.59

Molecular weight: 184.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4GTF2|A0A0Q4GTF2_9SPHI Aspartate aminotransferase OS=Pedobacter sp. Leaf216 OX=1735684 GN=ASE74_19645 PE=4 SV=1
MM1 pKa = 7.65GIIIKK6 pKa = 10.49SGGIIIKK13 pKa = 10.35SRR15 pKa = 11.84GIIIKK20 pKa = 10.42SGGIIIKK27 pKa = 10.37SGGVIIKK34 pKa = 10.41SGGIIIKK41 pKa = 10.42SGGIIIKK48 pKa = 10.35SRR50 pKa = 11.84GIIIKK55 pKa = 10.37SGGVIIKK62 pKa = 10.41SGGIIIKK69 pKa = 10.42SGGIIIKK76 pKa = 10.42SGGIIIKK83 pKa = 10.42SGGIIIKK90 pKa = 10.35SRR92 pKa = 11.84GIIIKK97 pKa = 10.29SRR99 pKa = 11.84ASLL102 pKa = 3.49

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4730

0

4730

1606351

36

4486

339.6

38.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.537 ± 0.036

0.729 ± 0.011

5.351 ± 0.025

5.598 ± 0.031

5.057 ± 0.03

6.806 ± 0.038

1.732 ± 0.017

7.493 ± 0.033

7.594 ± 0.042

9.401 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.016

6.073 ± 0.034

3.679 ± 0.022

3.566 ± 0.023

3.553 ± 0.024

6.337 ± 0.029

5.881 ± 0.066

6.163 ± 0.029

1.162 ± 0.015

4.134 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski