Amino acid dipepetide frequency for Pedobacter sp. Leaf216

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.271AlaAla: 6.271 ± 0.078	0.633AlaCys: 0.633 ± 0.025	4.503AlaAsp: 4.503 ± 0.061	4.597AlaGlu: 4.597 ± 0.057	3.631AlaPhe: 3.631 ± 0.05	5.508AlaGly: 5.508 ± 0.081	1.236AlaHis: 1.236 ± 0.031	5.695AlaIle: 5.695 ± 0.064	5.446AlaLys: 5.446 ± 0.068	7.022AlaLeu: 7.022 ± 0.086	1.596AlaMet: 1.596 ± 0.042	4.19AlaAsn: 4.19 ± 0.051	2.272AlaPro: 2.272 ± 0.049	3.022AlaGln: 3.022 ± 0.05	2.241AlaArg: 2.241 ± 0.043	4.507AlaSer: 4.507 ± 0.061	4.416AlaThr: 4.416 ± 0.132	4.766AlaVal: 4.766 ± 0.062	0.786AlaTrp: 0.786 ± 0.022	3.027AlaTyr: 3.027 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.486CysAla: 0.486 ± 0.018	0.118CysCys: 0.118 ± 0.009	0.347CysAsp: 0.347 ± 0.016	0.354CysGlu: 0.354 ± 0.016	0.43CysPhe: 0.43 ± 0.016	0.605CysGly: 0.605 ± 0.021	0.181CysHis: 0.181 ± 0.012	0.619CysIle: 0.619 ± 0.021	0.536CysLys: 0.536 ± 0.018	0.687CysLeu: 0.687 ± 0.02	0.159CysMet: 0.159 ± 0.011	0.372CysAsn: 0.372 ± 0.016	0.278CysPro: 0.278 ± 0.014	0.192CysGln: 0.192 ± 0.011	0.268CysArg: 0.268 ± 0.014	0.474CysSer: 0.474 ± 0.02	0.413CysThr: 0.413 ± 0.018	0.401CysVal: 0.401 ± 0.016	0.068CysTrp: 0.068 ± 0.006	0.299CysTyr: 0.299 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	4.093AspAla: 4.093 ± 0.059	0.375AspCys: 0.375 ± 0.016	2.588AspAsp: 2.588 ± 0.041	3.4AspGlu: 3.4 ± 0.05	3.281AspPhe: 3.281 ± 0.047	3.964AspGly: 3.964 ± 0.056	1.021AspHis: 1.021 ± 0.028	3.832AspIle: 3.832 ± 0.05	4.071AspLys: 4.071 ± 0.049	5.165AspLeu: 5.165 ± 0.057	1.127AspMet: 1.127 ± 0.028	2.658AspAsn: 2.658 ± 0.053	1.962AspPro: 1.962 ± 0.038	1.96AspGln: 1.96 ± 0.039	2.046AspArg: 2.046 ± 0.038	2.646AspSer: 2.646 ± 0.054	2.438AspThr: 2.438 ± 0.051	3.456AspVal: 3.456 ± 0.047	0.802AspTrp: 0.802 ± 0.025	2.623AspTyr: 2.623 ± 0.044	0.001AspXaa: 0.001 ± 0.001
Glu	3.882GluAla: 3.882 ± 0.051	0.293GluCys: 0.293 ± 0.015	2.761GluAsp: 2.761 ± 0.042	3.626GluGlu: 3.626 ± 0.062	2.409GluPhe: 2.409 ± 0.04	3.174GluGly: 3.174 ± 0.051	1.046GluHis: 1.046 ± 0.028	4.678GluIle: 4.678 ± 0.068	5.022GluLys: 5.022 ± 0.076	5.422GluLeu: 5.422 ± 0.071	1.416GluMet: 1.416 ± 0.033	3.705GluAsn: 3.705 ± 0.05	1.51GluPro: 1.51 ± 0.031	2.265GluGln: 2.265 ± 0.039	2.31GluArg: 2.31 ± 0.044	2.996GluSer: 2.996 ± 0.055	3.103GluThr: 3.103 ± 0.045	3.789GluVal: 3.789 ± 0.062	0.623GluTrp: 0.623 ± 0.018	1.95GluTyr: 1.95 ± 0.039	0.001GluXaa: 0.001 ± 0.001
Phe	3.652PheAla: 3.652 ± 0.053	0.474PheCys: 0.474 ± 0.018	2.992PheAsp: 2.992 ± 0.039	2.809PheGlu: 2.809 ± 0.045	2.549PhePhe: 2.549 ± 0.054	3.573PheGly: 3.573 ± 0.06	0.761PheHis: 0.761 ± 0.022	3.745PheIle: 3.745 ± 0.059	3.693PheLys: 3.693 ± 0.05	4.515PheLeu: 4.515 ± 0.067	1.048PheMet: 1.048 ± 0.027	3.271PheAsn: 3.271 ± 0.044	1.7PhePro: 1.7 ± 0.033	1.335PheGln: 1.335 ± 0.033	1.707PheArg: 1.707 ± 0.035	3.883PheSer: 3.883 ± 0.053	3.186PheThr: 3.186 ± 0.057	2.81PheVal: 2.81 ± 0.047	0.614PheTrp: 0.614 ± 0.023	2.254PheTyr: 2.254 ± 0.04	0.001PheXaa: 0.001 ± 0.001
Gly	4.72GlyAla: 4.72 ± 0.068	0.584GlyCys: 0.584 ± 0.021	3.322GlyAsp: 3.322 ± 0.055	3.372GlyGlu: 3.372 ± 0.047	3.823GlyPhe: 3.823 ± 0.048	4.929GlyGly: 4.929 ± 0.084	1.126GlyHis: 1.126 ± 0.027	5.455GlyIle: 5.455 ± 0.069	5.724GlyLys: 5.724 ± 0.074	6.304GlyLeu: 6.304 ± 0.071	1.579GlyMet: 1.579 ± 0.032	4.06GlyAsn: 4.06 ± 0.063	1.505GlyPro: 1.505 ± 0.038	2.123GlyGln: 2.123 ± 0.045	2.402GlyArg: 2.402 ± 0.045	4.463GlySer: 4.463 ± 0.063	4.377GlyThr: 4.377 ± 0.106	4.158GlyVal: 4.158 ± 0.058	0.92GlyTrp: 0.92 ± 0.028	3.118GlyTyr: 3.118 ± 0.054	0.001GlyXaa: 0.001 ± 0.001
His	1.116HisAla: 1.116 ± 0.03	0.145HisCys: 0.145 ± 0.009	0.838HisAsp: 0.838 ± 0.029	0.927HisGlu: 0.927 ± 0.025	1.079HisPhe: 1.079 ± 0.029	1.091HisGly: 1.091 ± 0.033	0.509HisHis: 0.509 ± 0.02	1.304HisIle: 1.304 ± 0.035	1.025HisLys: 1.025 ± 0.03	1.807HisLeu: 1.807 ± 0.033	0.329HisMet: 0.329 ± 0.014	0.934HisAsn: 0.934 ± 0.022	0.931HisPro: 0.931 ± 0.026	0.815HisGln: 0.815 ± 0.022	0.67HisArg: 0.67 ± 0.02	0.944HisSer: 0.944 ± 0.027	0.916HisThr: 0.916 ± 0.025	0.874HisVal: 0.874 ± 0.025	0.228HisTrp: 0.228 ± 0.012	0.834HisTyr: 0.834 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.006IleAla: 6.006 ± 0.066	0.682IleCys: 0.682 ± 0.023	4.384IleAsp: 4.384 ± 0.046	4.351IleGlu: 4.351 ± 0.055	3.472IlePhe: 3.472 ± 0.062	5.069IleGly: 5.069 ± 0.064	1.191IleHis: 1.191 ± 0.029	5.397IleIle: 5.397 ± 0.076	5.708IleLys: 5.708 ± 0.069	6.596IleLeu: 6.596 ± 0.083	1.334IleMet: 1.334 ± 0.03	4.817IleAsn: 4.817 ± 0.059	3.073IlePro: 3.073 ± 0.046	2.264IleGln: 2.264 ± 0.037	2.699IleArg: 2.699 ± 0.04	5.468IleSer: 5.468 ± 0.073	4.633IleThr: 4.633 ± 0.068	4.278IleVal: 4.278 ± 0.06	0.728IleTrp: 0.728 ± 0.022	2.778IleTyr: 2.778 ± 0.044	0.003IleXaa: 0.003 ± 0.001
Lys	5.701LysAla: 5.701 ± 0.067	0.317LysCys: 0.317 ± 0.016	4.279LysAsp: 4.279 ± 0.057	4.602LysGlu: 4.602 ± 0.069	2.972LysPhe: 2.972 ± 0.046	4.809LysGly: 4.809 ± 0.062	1.345LysHis: 1.345 ± 0.032	5.747LysIle: 5.747 ± 0.061	6.1LysLys: 6.1 ± 0.084	6.841LysLeu: 6.841 ± 0.074	1.969LysMet: 1.969 ± 0.037	5.041LysAsn: 5.041 ± 0.057	2.949LysPro: 2.949 ± 0.042	2.851LysGln: 2.851 ± 0.045	2.683LysArg: 2.683 ± 0.044	4.411LysSer: 4.411 ± 0.062	4.758LysThr: 4.758 ± 0.058	4.581LysVal: 4.581 ± 0.058	0.908LysTrp: 0.908 ± 0.026	3.071LysTyr: 3.071 ± 0.047	0.003LysXaa: 0.003 ± 0.002
Leu	7.12LeuAla: 7.12 ± 0.084	0.715LeuCys: 0.715 ± 0.022	4.683LeuAsp: 4.683 ± 0.054	4.717LeuGlu: 4.717 ± 0.065	4.619LeuPhe: 4.619 ± 0.059	5.883LeuGly: 5.883 ± 0.07	1.556LeuHis: 1.556 ± 0.034	6.951LeuIle: 6.951 ± 0.087	7.863LeuLys: 7.863 ± 0.07	8.867LeuLeu: 8.867 ± 0.115	2.084LeuMet: 2.084 ± 0.043	6.088LeuAsn: 6.088 ± 0.07	3.967LeuPro: 3.967 ± 0.053	3.398LeuGln: 3.398 ± 0.05	3.337LeuArg: 3.337 ± 0.048	7.111LeuSer: 7.111 ± 0.064	5.677LeuThr: 5.677 ± 0.07	5.27LeuVal: 5.27 ± 0.056	0.908LeuTrp: 0.908 ± 0.03	3.197LeuTyr: 3.197 ± 0.049	0.002LeuXaa: 0.002 ± 0.001
Met	1.735MetAla: 1.735 ± 0.034	0.136MetCys: 0.136 ± 0.01	1.166MetAsp: 1.166 ± 0.03	1.319MetGlu: 1.319 ± 0.034	0.839MetPhe: 0.839 ± 0.023	1.462MetGly: 1.462 ± 0.036	0.398MetHis: 0.398 ± 0.016	1.472MetIle: 1.472 ± 0.038	1.947MetLys: 1.947 ± 0.036	2.044MetLeu: 2.044 ± 0.039	0.538MetMet: 0.538 ± 0.018	1.176MetAsn: 1.176 ± 0.025	1.004MetPro: 1.004 ± 0.03	0.962MetGln: 0.962 ± 0.026	0.855MetArg: 0.855 ± 0.025	1.223MetSer: 1.223 ± 0.028	0.955MetThr: 0.955 ± 0.024	1.46MetVal: 1.46 ± 0.031	0.186MetTrp: 0.186 ± 0.011	0.631MetTyr: 0.631 ± 0.02	0.001MetXaa: 0.001 ± 0.001
Asn	4.473AsnAla: 4.473 ± 0.057	0.428AsnCys: 0.428 ± 0.018	2.977AsnAsp: 2.977 ± 0.047	3.173AsnGlu: 3.173 ± 0.048	3.098AsnPhe: 3.098 ± 0.049	4.403AsnGly: 4.403 ± 0.072	1.094AsnHis: 1.094 ± 0.03	4.581AsnIle: 4.581 ± 0.064	4.107AsnLys: 4.107 ± 0.055	5.654AsnLeu: 5.654 ± 0.076	1.216AsnMet: 1.216 ± 0.029	3.764AsnAsn: 3.764 ± 0.063	2.9AsnPro: 2.9 ± 0.053	2.374AsnGln: 2.374 ± 0.041	2.33AsnArg: 2.33 ± 0.041	3.43AsnSer: 3.43 ± 0.056	3.557AsnThr: 3.557 ± 0.06	3.477AsnVal: 3.477 ± 0.053	0.83AsnTrp: 0.83 ± 0.022	2.857AsnTyr: 2.857 ± 0.046	0.001AsnXaa: 0.001 ± 0.001
Pro	3.075ProAla: 3.075 ± 0.063	0.192ProCys: 0.192 ± 0.011	2.35ProAsp: 2.35 ± 0.043	2.695ProGlu: 2.695 ± 0.044	1.876ProPhe: 1.876 ± 0.032	2.562ProGly: 2.562 ± 0.04	0.62ProHis: 0.62 ± 0.023	2.488ProIle: 2.488 ± 0.04	2.493ProLys: 2.493 ± 0.042	3.215ProLeu: 3.215 ± 0.049	0.76ProMet: 0.76 ± 0.022	2.136ProAsn: 2.136 ± 0.041	0.931ProPro: 0.931 ± 0.03	1.292ProGln: 1.292 ± 0.028	0.989ProArg: 0.989 ± 0.028	2.215ProSer: 2.215 ± 0.038	2.108ProThr: 2.108 ± 0.05	2.94ProVal: 2.94 ± 0.061	0.392ProTrp: 0.392 ± 0.017	1.462ProTyr: 1.462 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	2.547GlnAla: 2.547 ± 0.041	0.173GlnCys: 0.173 ± 0.01	1.688GlnAsp: 1.688 ± 0.034	1.889GlnGlu: 1.889 ± 0.039	1.659GlnPhe: 1.659 ± 0.034	1.988GlnGly: 1.988 ± 0.041	0.702GlnHis: 0.702 ± 0.023	2.585GlnIle: 2.585 ± 0.039	2.762GlnLys: 2.762 ± 0.045	3.576GlnLeu: 3.576 ± 0.052	0.834GlnMet: 0.834 ± 0.026	2.32GlnAsn: 2.32 ± 0.04	1.352GlnPro: 1.352 ± 0.027	1.812GlnGln: 1.812 ± 0.059	1.396GlnArg: 1.396 ± 0.039	2.176GlnSer: 2.176 ± 0.039	2.059GlnThr: 2.059 ± 0.041	2.257GlnVal: 2.257 ± 0.044	0.38GlnTrp: 0.38 ± 0.016	1.507GlnTyr: 1.507 ± 0.028	0.001GlnXaa: 0.001 ± 0.001
Arg	2.288ArgAla: 2.288 ± 0.043	0.219ArgCys: 0.219 ± 0.011	1.822ArgAsp: 1.822 ± 0.032	1.958ArgGlu: 1.958 ± 0.04	2.081ArgPhe: 2.081 ± 0.034	2.032ArgGly: 2.032 ± 0.038	0.533ArgHis: 0.533 ± 0.018	2.936ArgIle: 2.936 ± 0.042	2.806ArgLys: 2.806 ± 0.05	3.557ArgLeu: 3.557 ± 0.045	0.936ArgMet: 0.936 ± 0.024	2.207ArgAsn: 2.207 ± 0.042	1.225ArgPro: 1.225 ± 0.03	1.193ArgGln: 1.193 ± 0.028	1.358ArgArg: 1.358 ± 0.033	2.132ArgSer: 2.132 ± 0.041	1.878ArgThr: 1.878 ± 0.034	2.148ArgVal: 2.148 ± 0.035	0.474ArgTrp: 0.474 ± 0.017	1.751ArgTyr: 1.751 ± 0.033	0.001ArgXaa: 0.001 ± 0.001
Ser	4.897SerAla: 4.897 ± 0.057	0.534SerCys: 0.534 ± 0.017	3.151SerAsp: 3.151 ± 0.049	3.092SerGlu: 3.092 ± 0.051	3.664SerPhe: 3.664 ± 0.054	4.856SerGly: 4.856 ± 0.066	1.004SerHis: 1.004 ± 0.027	4.995SerIle: 4.995 ± 0.062	4.274SerLys: 4.274 ± 0.053	6.171SerLeu: 6.171 ± 0.066	1.228SerMet: 1.228 ± 0.026	3.486SerAsn: 3.486 ± 0.058	2.32SerPro: 2.32 ± 0.04	1.831SerGln: 1.831 ± 0.034	2.263SerArg: 2.263 ± 0.043	4.167SerSer: 4.167 ± 0.061	3.783SerThr: 3.783 ± 0.063	4.1SerVal: 4.1 ± 0.053	0.756SerTrp: 0.756 ± 0.025	2.793SerTyr: 2.793 ± 0.051	0.007SerXaa: 0.007 ± 0.002
Thr	4.932ThrAla: 4.932 ± 0.108	0.332ThrCys: 0.332 ± 0.016	3.52ThrAsp: 3.52 ± 0.062	3.166ThrGlu: 3.166 ± 0.051	2.966ThrPhe: 2.966 ± 0.059	4.651ThrGly: 4.651 ± 0.075	0.9ThrHis: 0.9 ± 0.026	4.437ThrIle: 4.437 ± 0.093	3.688ThrLys: 3.688 ± 0.049	5.595ThrLeu: 5.595 ± 0.082	0.949ThrMet: 0.949 ± 0.022	3.167ThrAsn: 3.167 ± 0.059	2.579ThrPro: 2.579 ± 0.063	1.876ThrGln: 1.876 ± 0.037	1.698ThrArg: 1.698 ± 0.032	3.57ThrSer: 3.57 ± 0.059	3.659ThrThr: 3.659 ± 0.106	4.033ThrVal: 4.033 ± 0.107	0.649ThrTrp: 0.649 ± 0.02	2.445ThrTyr: 2.445 ± 0.053	0.002ThrXaa: 0.002 ± 0.001
Val	4.608ValAla: 4.608 ± 0.063	0.512ValCys: 0.512 ± 0.018	3.33ValAsp: 3.33 ± 0.049	3.327ValGlu: 3.327 ± 0.053	3.132ValPhe: 3.132 ± 0.045	3.797ValGly: 3.797 ± 0.052	1.005ValHis: 1.005 ± 0.027	4.632ValIle: 4.632 ± 0.06	4.877ValLys: 4.877 ± 0.063	5.9ValLeu: 5.9 ± 0.07	1.349ValMet: 1.349 ± 0.031	3.928ValAsn: 3.928 ± 0.059	2.268ValPro: 2.268 ± 0.044	1.859ValGln: 1.859 ± 0.032	2.056ValArg: 2.056 ± 0.04	4.201ValSer: 4.201 ± 0.062	3.741ValThr: 3.741 ± 0.104	4.061ValVal: 4.061 ± 0.054	0.639ValTrp: 0.639 ± 0.021	2.411ValTyr: 2.411 ± 0.04	0.001ValXaa: 0.001 ± 0.001
Trp	0.787TrpAla: 0.787 ± 0.025	0.108TrpCys: 0.108 ± 0.009	0.634TrpAsp: 0.634 ± 0.021	0.643TrpGlu: 0.643 ± 0.021	0.604TrpPhe: 0.604 ± 0.022	0.826TrpGly: 0.826 ± 0.024	0.255TrpHis: 0.255 ± 0.013	0.763TrpIle: 0.763 ± 0.027	0.893TrpLys: 0.893 ± 0.025	1.138TrpLeu: 1.138 ± 0.032	0.325TrpMet: 0.325 ± 0.015	0.7TrpAsn: 0.7 ± 0.024	0.39TrpPro: 0.39 ± 0.017	0.482TrpGln: 0.482 ± 0.018	0.461TrpArg: 0.461 ± 0.016	0.661TrpSer: 0.661 ± 0.02	0.631TrpThr: 0.631 ± 0.02	0.659TrpVal: 0.659 ± 0.021	0.19TrpTrp: 0.19 ± 0.011	0.471TrpTyr: 0.471 ± 0.024	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.973TyrAla: 2.973 ± 0.045	0.318TyrCys: 0.318 ± 0.014	2.176TyrAsp: 2.176 ± 0.042	1.962TyrGlu: 1.962 ± 0.037	2.39TyrPhe: 2.39 ± 0.043	2.879TyrGly: 2.879 ± 0.048	0.832TyrHis: 0.832 ± 0.024	2.619TyrIle: 2.619 ± 0.044	2.901TyrLys: 2.901 ± 0.039	3.933TyrLeu: 3.933 ± 0.052	0.734TyrMet: 0.734 ± 0.023	2.709TyrAsn: 2.709 ± 0.052	1.676TyrPro: 1.676 ± 0.032	1.756TyrGln: 1.756 ± 0.037	1.762TyrArg: 1.762 ± 0.034	2.691TyrSer: 2.691 ± 0.046	2.519TyrThr: 2.519 ± 0.054	2.115TyrVal: 2.115 ± 0.043	0.539TyrTrp: 0.539 ± 0.019	1.853TyrTyr: 1.853 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.004XaaThr: 0.004 ± 0.002	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.002XaaTyr: 0.002 ± 0.001	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4730 proteins (1606351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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