Cordyceps fumosorosea (strain ARSEF 2679) (Isaria fumosorosea)
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10050 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162MRH4|A0A162MRH4_CORFA Carnitine O-acetyltransferase OS=Cordyceps fumosorosea (strain ARSEF 2679) OX=1081104 GN=ISF_03444 PE=3 SV=1
MM1 pKa = 7.57 TEE3 pKa = 3.59 TATAAGQQCTVLYY16 pKa = 9.83 FAGASSYY23 pKa = 11.19 AGRR26 pKa = 11.84 DD27 pKa = 3.6 SEE29 pKa = 5.13 SFPAPVPLGQLLAAVEE45 pKa = 4.06 ARR47 pKa = 11.84 HH48 pKa = 5.62 PGIRR52 pKa = 11.84 GAVLDD57 pKa = 4.03 ACLVTVNLDD66 pKa = 3.79 YY67 pKa = 11.61 VDD69 pKa = 4.22 VPAAGDD75 pKa = 3.69 EE76 pKa = 4.41 GVLIQTADD84 pKa = 3.58 EE85 pKa = 4.24 VAIIPPVSSGG95 pKa = 3.12
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.986
IPC_protein 3.859
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.783
Nozaki 3.999
DTASelect 4.164
Thurlkill 3.745
EMBOSS 3.808
Sillero 3.986
Patrickios 0.693
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|A0A168BMA7|A0A168BMA7_CORFA Uncharacterized protein OS=Cordyceps fumosorosea (strain ARSEF 2679) OX=1081104 GN=ISF_02279 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.46 FSVVLLAAAATSVVGQSATNSSSRR26 pKa = 11.84 STTAVASSTSARR38 pKa = 11.84 SSASGSATSRR48 pKa = 11.84 SASGSATTTARR59 pKa = 11.84 SSSVSKK65 pKa = 11.03 AGAAPTNAARR75 pKa = 11.84 AGAGVAAVAVGVAAMMVV92 pKa = 3.25
Molecular weight: 8.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.281
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10050
0
10050
4864145
49
5325
484.0
53.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.181 ± 0.027
1.232 ± 0.011
5.889 ± 0.018
5.922 ± 0.021
3.55 ± 0.016
7.258 ± 0.024
2.378 ± 0.011
4.32 ± 0.018
4.446 ± 0.022
8.876 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.198 ± 0.009
3.309 ± 0.014
6.064 ± 0.026
3.921 ± 0.019
6.507 ± 0.024
7.723 ± 0.028
5.808 ± 0.019
6.383 ± 0.018
1.436 ± 0.011
2.598 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here