Clostridium acetireducens DSM 10703

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium acetireducens

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E8EZN5|A0A1E8EZN5_9CLOT DNA replication protein DnaC OS=Clostridium acetireducens DSM 10703 OX=1121290 GN=dnaC_1 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 5.82NIIDD6 pKa = 3.76ILVIEE11 pKa = 5.69DD12 pKa = 3.49DD13 pKa = 4.0TNINKK18 pKa = 9.29LLCDD22 pKa = 3.53MLSNSGYY29 pKa = 8.47KK30 pKa = 9.78TIAAYY35 pKa = 10.52SGTEE39 pKa = 3.55ALLYY43 pKa = 8.09IQKK46 pKa = 10.34YY47 pKa = 7.76EE48 pKa = 4.46LDD50 pKa = 4.35MILLDD55 pKa = 4.86LMLPGISGEE64 pKa = 4.03EE65 pKa = 3.91LLIKK69 pKa = 10.58

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E8EXX9|A0A1E8EXX9_9CLOT Big_5 domain-containing protein OS=Clostridium acetireducens DSM 10703 OX=1121290 GN=CLOACE_15170 PE=4 SV=1
MM1 pKa = 7.45WMTYY5 pKa = 7.14QPKK8 pKa = 9.67KK9 pKa = 8.81RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.08GFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MRR23 pKa = 11.84TLSGRR28 pKa = 11.84KK29 pKa = 7.2VLKK32 pKa = 10.1RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2237

0

2237

683161

29

2865

305.4

34.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.203 ± 0.055

1.297 ± 0.022

5.231 ± 0.041

7.445 ± 0.062

4.349 ± 0.042

6.003 ± 0.046

1.263 ± 0.017

10.676 ± 0.064

10.553 ± 0.062

8.825 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.024

7.103 ± 0.053

2.713 ± 0.028

2.197 ± 0.02

3.096 ± 0.033

6.042 ± 0.038

4.428 ± 0.035

6.241 ± 0.044

0.605 ± 0.016

4.277 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski