Amino acid dipepetide frequency for Clostridium acetireducens DSM 10703

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.695AlaAla: 3.695 ± 0.114
0.698AlaCys: 0.698 ± 0.035
2.294AlaAsp: 2.294 ± 0.069
3.235AlaGlu: 3.235 ± 0.075
2.361AlaPhe: 2.361 ± 0.07
3.267AlaGly: 3.267 ± 0.085
0.755AlaHis: 0.755 ± 0.033
5.51AlaIle: 5.51 ± 0.112
4.974AlaLys: 4.974 ± 0.104
5.542AlaLeu: 5.542 ± 0.094
1.366AlaMet: 1.366 ± 0.044
2.51AlaAsn: 2.51 ± 0.066
1.348AlaPro: 1.348 ± 0.048
1.24AlaGln: 1.24 ± 0.043
1.648AlaArg: 1.648 ± 0.056
3.106AlaSer: 3.106 ± 0.08
2.262AlaThr: 2.262 ± 0.072
3.876AlaVal: 3.876 ± 0.093
0.296AlaTrp: 0.296 ± 0.023
2.049AlaTyr: 2.049 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.635CysAla: 0.635 ± 0.032
0.228CysCys: 0.228 ± 0.02
0.644CysAsp: 0.644 ± 0.03
0.84CysGlu: 0.84 ± 0.041
0.512CysPhe: 0.512 ± 0.031
1.13CysGly: 1.13 ± 0.045
0.23CysHis: 0.23 ± 0.021
1.435CysIle: 1.435 ± 0.048
1.278CysLys: 1.278 ± 0.048
0.9CysLeu: 0.9 ± 0.037
0.307CysMet: 0.307 ± 0.022
0.931CysAsn: 0.931 ± 0.04
0.502CysPro: 0.502 ± 0.031
0.222CysGln: 0.222 ± 0.018
0.386CysArg: 0.386 ± 0.024
0.896CysSer: 0.896 ± 0.036
0.606CysThr: 0.606 ± 0.029
0.7CysVal: 0.7 ± 0.029
0.064CysTrp: 0.064 ± 0.01
0.525CysTyr: 0.525 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
2.44AspAla: 2.44 ± 0.076
0.58AspCys: 0.58 ± 0.035
2.288AspAsp: 2.288 ± 0.062
4.143AspGlu: 4.143 ± 0.075
2.869AspPhe: 2.869 ± 0.066
2.655AspGly: 2.655 ± 0.074
0.43AspHis: 0.43 ± 0.024
6.641AspIle: 6.641 ± 0.117
6.067AspLys: 6.067 ± 0.106
4.801AspLeu: 4.801 ± 0.089
1.389AspMet: 1.389 ± 0.053
3.465AspAsn: 3.465 ± 0.073
1.047AspPro: 1.047 ± 0.042
0.568AspGln: 0.568 ± 0.03
1.619AspArg: 1.619 ± 0.051
2.796AspSer: 2.796 ± 0.06
2.226AspThr: 2.226 ± 0.065
3.416AspVal: 3.416 ± 0.073
0.296AspTrp: 0.296 ± 0.022
2.573AspTyr: 2.573 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
3.768GluAla: 3.768 ± 0.083
0.77GluCys: 0.77 ± 0.033
4.473GluAsp: 4.473 ± 0.085
7.253GluGlu: 7.253 ± 0.129
3.149GluPhe: 3.149 ± 0.061
3.983GluGly: 3.983 ± 0.074
0.809GluHis: 0.809 ± 0.034
7.676GluIle: 7.676 ± 0.111
8.753GluLys: 8.753 ± 0.16
6.236GluLeu: 6.236 ± 0.104
1.796GluMet: 1.796 ± 0.052
6.187GluAsn: 6.187 ± 0.108
1.3GluPro: 1.3 ± 0.046
1.623GluGln: 1.623 ± 0.055
2.363GluArg: 2.363 ± 0.066
3.534GluSer: 3.534 ± 0.078
2.685GluThr: 2.685 ± 0.064
4.646GluVal: 4.646 ± 0.087
0.378GluTrp: 0.378 ± 0.026
3.072GluTyr: 3.072 ± 0.072
0.0GluXaa: 0.0 ± 0.0
Phe
2.127PheAla: 2.127 ± 0.054
0.612PheCys: 0.612 ± 0.029
2.221PheAsp: 2.221 ± 0.057
2.562PheGlu: 2.562 ± 0.068
1.878PhePhe: 1.878 ± 0.059
2.613PheGly: 2.613 ± 0.071
0.578PheHis: 0.578 ± 0.03
4.958PheIle: 4.958 ± 0.109
4.466PheLys: 4.466 ± 0.094
3.755PheLeu: 3.755 ± 0.097
1.07PheMet: 1.07 ± 0.039
3.411PheAsn: 3.411 ± 0.081
1.174PhePro: 1.174 ± 0.047
1.093PheGln: 1.093 ± 0.034
1.139PheArg: 1.139 ± 0.048
3.102PheSer: 3.102 ± 0.073
2.114PheThr: 2.114 ± 0.052
2.452PheVal: 2.452 ± 0.068
0.283PheTrp: 0.283 ± 0.022
1.888PheTyr: 1.888 ± 0.061
0.0PheXaa: 0.0 ± 0.0
Gly
3.62GlyAla: 3.62 ± 0.114
0.891GlyCys: 0.891 ± 0.04
3.03GlyAsp: 3.03 ± 0.064
4.107GlyGlu: 4.107 ± 0.088
2.847GlyPhe: 2.847 ± 0.064
3.879GlyGly: 3.879 ± 0.099
0.931GlyHis: 0.931 ± 0.04
6.619GlyIle: 6.619 ± 0.114
6.231GlyLys: 6.231 ± 0.099
4.697GlyLeu: 4.697 ± 0.101
1.631GlyMet: 1.631 ± 0.054
3.269GlyAsn: 3.269 ± 0.071
1.177GlyPro: 1.177 ± 0.05
1.301GlyGln: 1.301 ± 0.045
2.111GlyArg: 2.111 ± 0.059
3.242GlySer: 3.242 ± 0.08
2.989GlyThr: 2.989 ± 0.07
4.248GlyVal: 4.248 ± 0.093
0.413GlyTrp: 0.413 ± 0.028
2.791GlyTyr: 2.791 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
0.593HisAla: 0.593 ± 0.03
0.22HisCys: 0.22 ± 0.019
0.549HisAsp: 0.549 ± 0.031
0.689HisGlu: 0.689 ± 0.035
0.575HisPhe: 0.575 ± 0.03
0.872HisGly: 0.872 ± 0.033
0.247HisHis: 0.247 ± 0.02
1.454HisIle: 1.454 ± 0.045
1.148HisLys: 1.148 ± 0.044
1.067HisLeu: 1.067 ± 0.041
0.294HisMet: 0.294 ± 0.021
0.867HisAsn: 0.867 ± 0.036
0.55HisPro: 0.55 ± 0.032
0.285HisGln: 0.285 ± 0.021
0.464HisArg: 0.464 ± 0.026
0.827HisSer: 0.827 ± 0.035
0.64HisThr: 0.64 ± 0.033
0.685HisVal: 0.685 ± 0.033
0.097HisTrp: 0.097 ± 0.011
0.511HisTyr: 0.511 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.712IleAla: 5.712 ± 0.112
1.433IleCys: 1.433 ± 0.044
6.063IleAsp: 6.063 ± 0.116
7.833IleGlu: 7.833 ± 0.124
4.314IlePhe: 4.314 ± 0.111
6.12IleGly: 6.12 ± 0.123
1.254IleHis: 1.254 ± 0.047
10.8IleIle: 10.8 ± 0.173
11.467IleLys: 11.467 ± 0.148
9.785IleLeu: 9.785 ± 0.155
2.437IleMet: 2.437 ± 0.064
7.89IleAsn: 7.89 ± 0.113
3.539IlePro: 3.539 ± 0.072
2.382IleGln: 2.382 ± 0.065
3.077IleArg: 3.077 ± 0.065
6.918IleSer: 6.918 ± 0.111
4.836IleThr: 4.836 ± 0.092
6.309IleVal: 6.309 ± 0.105
0.521IleTrp: 0.521 ± 0.03
4.069IleTyr: 4.069 ± 0.09
0.0IleXaa: 0.0 ± 0.0
Lys
4.866LysAla: 4.866 ± 0.094
1.218LysCys: 1.218 ± 0.043
6.837LysAsp: 6.837 ± 0.111
10.304LysGlu: 10.304 ± 0.156
3.904LysPhe: 3.904 ± 0.073
5.961LysGly: 5.961 ± 0.1
1.24LysHis: 1.24 ± 0.04
10.44LysIle: 10.44 ± 0.11
10.554LysLys: 10.554 ± 0.166
8.619LysLeu: 8.619 ± 0.132
2.507LysMet: 2.507 ± 0.062
8.756LysAsn: 8.756 ± 0.139
2.307LysPro: 2.307 ± 0.064
2.404LysGln: 2.404 ± 0.067
3.118LysArg: 3.118 ± 0.071
5.968LysSer: 5.968 ± 0.096
4.096LysThr: 4.096 ± 0.082
6.589LysVal: 6.589 ± 0.11
0.64LysTrp: 0.64 ± 0.032
5.207LysTyr: 5.207 ± 0.114
0.0LysXaa: 0.0 ± 0.0
Leu
4.669LeuAla: 4.669 ± 0.087
1.136LeuCys: 1.136 ± 0.042
4.889LeuAsp: 4.889 ± 0.088
6.315LeuGlu: 6.315 ± 0.125
3.526LeuPhe: 3.526 ± 0.095
5.767LeuGly: 5.767 ± 0.118
1.05LeuHis: 1.05 ± 0.044
8.304LeuIle: 8.304 ± 0.131
9.92LeuLys: 9.92 ± 0.131
7.331LeuLeu: 7.331 ± 0.146
2.071LeuMet: 2.071 ± 0.058
6.799LeuAsn: 6.799 ± 0.116
2.679LeuPro: 2.679 ± 0.062
1.995LeuGln: 1.995 ± 0.056
2.781LeuArg: 2.781 ± 0.074
6.094LeuSer: 6.094 ± 0.12
3.955LeuThr: 3.955 ± 0.093
5.113LeuVal: 5.113 ± 0.089
0.515LeuTrp: 0.515 ± 0.029
3.336LeuTyr: 3.336 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
1.591MetAla: 1.591 ± 0.049
0.28MetCys: 0.28 ± 0.02
1.496MetAsp: 1.496 ± 0.053
1.997MetGlu: 1.997 ± 0.057
0.973MetPhe: 0.973 ± 0.036
1.628MetGly: 1.628 ± 0.06
0.319MetHis: 0.319 ± 0.023
1.963MetIle: 1.963 ± 0.059
2.62MetLys: 2.62 ± 0.062
2.178MetLeu: 2.178 ± 0.06
0.509MetMet: 0.509 ± 0.028
1.637MetAsn: 1.637 ± 0.05
0.858MetPro: 0.858 ± 0.039
0.653MetGln: 0.653 ± 0.031
0.771MetArg: 0.771 ± 0.031
1.458MetSer: 1.458 ± 0.045
1.044MetThr: 1.044 ± 0.041
1.396MetVal: 1.396 ± 0.047
0.155MetTrp: 0.155 ± 0.016
0.988MetTyr: 0.988 ± 0.041
0.0MetXaa: 0.0 ± 0.0
Asn
2.84AsnAla: 2.84 ± 0.072
0.99AsnCys: 0.99 ± 0.041
2.695AsnAsp: 2.695 ± 0.07
4.598AsnGlu: 4.598 ± 0.093
3.106AsnPhe: 3.106 ± 0.073
3.214AsnGly: 3.214 ± 0.071
0.808AsnHis: 0.808 ± 0.032
9.21AsnIle: 9.21 ± 0.149
8.431AsnLys: 8.431 ± 0.133
6.511AsnLeu: 6.511 ± 0.122
1.833AsnMet: 1.833 ± 0.054
5.719AsnAsn: 5.719 ± 0.139
2.209AsnPro: 2.209 ± 0.059
1.414AsnGln: 1.414 ± 0.045
2.068AsnArg: 2.068 ± 0.056
4.337AsnSer: 4.337 ± 0.094
3.133AsnThr: 3.133 ± 0.07
4.119AsnVal: 4.119 ± 0.085
0.514AsnTrp: 0.514 ± 0.03
3.28AsnTyr: 3.28 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
1.316ProAla: 1.316 ± 0.051
0.379ProCys: 0.379 ± 0.026
1.285ProAsp: 1.285 ± 0.047
2.197ProGlu: 2.197 ± 0.054
1.341ProPhe: 1.341 ± 0.047
1.711ProGly: 1.711 ± 0.062
0.427ProHis: 0.427 ± 0.025
2.781ProIle: 2.781 ± 0.074
2.575ProLys: 2.575 ± 0.064
2.447ProLeu: 2.447 ± 0.062
0.615ProMet: 0.615 ± 0.032
1.622ProAsn: 1.622 ± 0.045
0.641ProPro: 0.641 ± 0.033
0.666ProGln: 0.666 ± 0.034
0.729ProArg: 0.729 ± 0.036
1.609ProSer: 1.609 ± 0.046
1.304ProThr: 1.304 ± 0.043
2.002ProVal: 2.002 ± 0.055
0.202ProTrp: 0.202 ± 0.019
1.282ProTyr: 1.282 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
1.225GlnAla: 1.225 ± 0.043
0.343GlnCys: 0.343 ± 0.021
1.093GlnAsp: 1.093 ± 0.037
1.538GlnGlu: 1.538 ± 0.051
0.899GlnPhe: 0.899 ± 0.034
1.427GlnGly: 1.427 ± 0.049
0.321GlnHis: 0.321 ± 0.023
2.177GlnIle: 2.177 ± 0.059
2.093GlnLys: 2.093 ± 0.067
1.937GlnLeu: 1.937 ± 0.056
0.571GlnMet: 0.571 ± 0.035
1.502GlnAsn: 1.502 ± 0.048
0.537GlnPro: 0.537 ± 0.029
0.698GlnGln: 0.698 ± 0.038
0.811GlnArg: 0.811 ± 0.038
1.256GlnSer: 1.256 ± 0.043
0.856GlnThr: 0.856 ± 0.036
1.456GlnVal: 1.456 ± 0.049
0.179GlnTrp: 0.179 ± 0.016
1.052GlnTyr: 1.052 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
1.68ArgAla: 1.68 ± 0.056
0.385ArgCys: 0.385 ± 0.028
1.672ArgAsp: 1.672 ± 0.05
2.74ArgGlu: 2.74 ± 0.073
1.353ArgPhe: 1.353 ± 0.046
1.881ArgGly: 1.881 ± 0.055
0.42ArgHis: 0.42 ± 0.026
3.029ArgIle: 3.029 ± 0.062
3.244ArgLys: 3.244 ± 0.07
2.565ArgLeu: 2.565 ± 0.062
0.831ArgMet: 0.831 ± 0.033
2.039ArgAsn: 2.039 ± 0.057
0.83ArgPro: 0.83 ± 0.04
0.849ArgGln: 0.849 ± 0.038
1.322ArgArg: 1.322 ± 0.045
1.306ArgSer: 1.306 ± 0.048
1.373ArgThr: 1.373 ± 0.049
2.013ArgVal: 2.013 ± 0.055
0.195ArgTrp: 0.195 ± 0.019
1.238ArgTyr: 1.238 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
2.816SerAla: 2.816 ± 0.07
0.719SerCys: 0.719 ± 0.035
2.471SerAsp: 2.471 ± 0.057
3.766SerGlu: 3.766 ± 0.089
2.808SerPhe: 2.808 ± 0.076
3.983SerGly: 3.983 ± 0.084
0.799SerHis: 0.799 ± 0.034
7.259SerIle: 7.259 ± 0.109
6.6SerLys: 6.6 ± 0.101
5.643SerLeu: 5.643 ± 0.103
1.576SerMet: 1.576 ± 0.048
4.14SerAsn: 4.14 ± 0.087
1.575SerPro: 1.575 ± 0.041
1.477SerGln: 1.477 ± 0.044
1.877SerArg: 1.877 ± 0.054
3.819SerSer: 3.819 ± 0.077
2.774SerThr: 2.774 ± 0.063
3.51SerVal: 3.51 ± 0.089
0.354SerTrp: 0.354 ± 0.027
2.458SerTyr: 2.458 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
2.758ThrAla: 2.758 ± 0.072
0.512ThrCys: 0.512 ± 0.029
2.068ThrAsp: 2.068 ± 0.06
2.689ThrGlu: 2.689 ± 0.063
1.942ThrPhe: 1.942 ± 0.053
3.222ThrGly: 3.222 ± 0.073
0.635ThrHis: 0.635 ± 0.032
4.813ThrIle: 4.813 ± 0.09
3.813ThrLys: 3.813 ± 0.071
4.413ThrLeu: 4.413 ± 0.09
1.039ThrMet: 1.039 ± 0.039
2.649ThrAsn: 2.649 ± 0.074
1.62ThrPro: 1.62 ± 0.051
0.868ThrGln: 0.868 ± 0.033
1.374ThrArg: 1.374 ± 0.05
2.81ThrSer: 2.81 ± 0.072
2.24ThrThr: 2.24 ± 0.071
2.914ThrVal: 2.914 ± 0.081
0.261ThrTrp: 0.261 ± 0.019
1.634ThrTyr: 1.634 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
3.465ValAla: 3.465 ± 0.098
0.905ValCys: 0.905 ± 0.04
3.646ValAsp: 3.646 ± 0.079
4.38ValGlu: 4.38 ± 0.081
2.86ValPhe: 2.86 ± 0.06
3.82ValGly: 3.82 ± 0.086
0.789ValHis: 0.789 ± 0.037
6.296ValIle: 6.296 ± 0.111
6.124ValLys: 6.124 ± 0.102
5.644ValLeu: 5.644 ± 0.102
1.443ValMet: 1.443 ± 0.048
3.834ValAsn: 3.834 ± 0.067
1.909ValPro: 1.909 ± 0.051
1.347ValGln: 1.347 ± 0.049
1.721ValArg: 1.721 ± 0.063
4.195ValSer: 4.195 ± 0.086
2.967ValThr: 2.967 ± 0.075
4.198ValVal: 4.198 ± 0.098
0.364ValTrp: 0.364 ± 0.026
2.503ValTyr: 2.503 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.337TrpAla: 0.337 ± 0.022
0.081TrpCys: 0.081 ± 0.012
0.332TrpAsp: 0.332 ± 0.02
0.395TrpGlu: 0.395 ± 0.025
0.258TrpPhe: 0.258 ± 0.018
0.379TrpGly: 0.379 ± 0.024
0.12TrpHis: 0.12 ± 0.014
0.656TrpIle: 0.656 ± 0.03
0.528TrpLys: 0.528 ± 0.027
0.48TrpLeu: 0.48 ± 0.03
0.174TrpMet: 0.174 ± 0.017
0.468TrpAsn: 0.468 ± 0.029
0.143TrpPro: 0.143 ± 0.014
0.164TrpGln: 0.164 ± 0.019
0.212TrpArg: 0.212 ± 0.018
0.375TrpSer: 0.375 ± 0.026
0.247TrpThr: 0.247 ± 0.02
0.382TrpVal: 0.382 ± 0.025
0.078TrpTrp: 0.078 ± 0.011
0.242TrpTyr: 0.242 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.88TyrAla: 1.88 ± 0.051
0.596TyrCys: 0.596 ± 0.028
2.263TyrAsp: 2.263 ± 0.06
2.873TyrGlu: 2.873 ± 0.069
2.019TyrPhe: 2.019 ± 0.053
2.512TyrGly: 2.512 ± 0.056
0.47TyrHis: 0.47 ± 0.025
4.741TyrIle: 4.741 ± 0.109
4.643TyrLys: 4.643 ± 0.101
3.695TyrLeu: 3.695 ± 0.083
1.051TyrMet: 1.051 ± 0.037
3.335TyrAsn: 3.335 ± 0.072
1.187TyrPro: 1.187 ± 0.039
0.722TyrGln: 0.722 ± 0.033
1.372TyrArg: 1.372 ± 0.046
2.777TyrSer: 2.777 ± 0.067
1.931TyrThr: 1.931 ± 0.052
2.386TyrVal: 2.386 ± 0.072
0.249TyrTrp: 0.249 ± 0.021
2.068TyrTyr: 2.068 ± 0.057
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2237 proteins (683161 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski