Marinobacter sp. AC-23
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3073 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S2CDY8|A0A1S2CDY8_9ALTE Uncharacterized protein OS=Marinobacter sp. AC-23 OX=1879031 GN=BCA33_02885 PE=4 SV=1
MM1 pKa = 7.3 KK2 pKa = 10.08 LKK4 pKa = 10.45 YY5 pKa = 9.73 SAAIICLALSPALVSAEE22 pKa = 4.05 PEE24 pKa = 4.05 AGDD27 pKa = 4.41 GLFALTGTGSSDD39 pKa = 3.52 NEE41 pKa = 3.78 FDD43 pKa = 3.85 NNVAAVSFDD52 pKa = 3.28 VGKK55 pKa = 10.54 FFSDD59 pKa = 2.99 NLAAGLRR66 pKa = 11.84 QSFGFADD73 pKa = 3.88 SEE75 pKa = 4.72 NNSSWSGASRR85 pKa = 11.84 GFVDD89 pKa = 3.01 YY90 pKa = 11.12 HH91 pKa = 7.2 FDD93 pKa = 3.65 GGSWQPFVGANLGGIYY109 pKa = 10.38 GDD111 pKa = 4.96 DD112 pKa = 3.41 VDD114 pKa = 4.06 EE115 pKa = 4.62 TFFAGPEE122 pKa = 4.04 AGVKK126 pKa = 10.15 YY127 pKa = 8.99 YY128 pKa = 10.75 VRR130 pKa = 11.84 EE131 pKa = 3.8 KK132 pKa = 10.52 TYY134 pKa = 9.25 ITVQMEE140 pKa = 4.12 YY141 pKa = 10.58 QVFFDD146 pKa = 4.23 SASDD150 pKa = 3.69 ADD152 pKa = 4.99 DD153 pKa = 4.61 NFDD156 pKa = 4.77 DD157 pKa = 5.12 GAFAYY162 pKa = 10.43 SAGIGYY168 pKa = 9.17 NFF170 pKa = 4.18
Molecular weight: 18.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.897
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A1S2CE99|A0A1S2CE99_9ALTE 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1 4-benzoquinol methylase OS=Marinobacter sp. AC-23 OX=1879031 GN=BCA33_02805 PE=4 SV=1
MM1 pKa = 7.35 NVTTIGIDD9 pKa = 3.26 LAKK12 pKa = 9.84 TVFSIHH18 pKa = 6.11 GTNQHH23 pKa = 5.19 GKK25 pKa = 8.42 VVVRR29 pKa = 11.84 KK30 pKa = 9.72 RR31 pKa = 11.84 LNRR34 pKa = 11.84 PKK36 pKa = 10.58 LLAFFAQLPPCLIGMEE52 pKa = 4.34 ACSGAHH58 pKa = 4.01 YY59 pKa = 8.21 WARR62 pKa = 11.84 EE63 pKa = 4.0 LTKK66 pKa = 10.39 LGHH69 pKa = 6.81 DD70 pKa = 3.89 ARR72 pKa = 11.84 IIAPRR77 pKa = 11.84 FVAPYY82 pKa = 9.82 RR83 pKa = 11.84 KK84 pKa = 9.62 SSKK87 pKa = 10.76 NDD89 pKa = 3.34 DD90 pKa = 3.64 NDD92 pKa = 3.81 AEE94 pKa = 4.76 AICEE98 pKa = 4.09 AVSRR102 pKa = 11.84 PNMRR106 pKa = 11.84 FVPIKK111 pKa = 9.78 TQDD114 pKa = 3.32 QQAALCLHH122 pKa = 7.63 RR123 pKa = 11.84 IRR125 pKa = 11.84 RR126 pKa = 11.84 GLIKK130 pKa = 10.46 EE131 pKa = 3.74 RR132 pKa = 11.84 TAQINQIRR140 pKa = 11.84 GLLAEE145 pKa = 5.25 FGLIIPQGRR154 pKa = 11.84 YY155 pKa = 7.89 HH156 pKa = 6.58 ARR158 pKa = 11.84 HH159 pKa = 6.91 RR160 pKa = 11.84 IPEE163 pKa = 4.03 ILEE166 pKa = 4.14 DD167 pKa = 4.23 AEE169 pKa = 4.71 NGLPMLARR177 pKa = 11.84 RR178 pKa = 11.84 LLNNINQRR186 pKa = 11.84 ILQLDD191 pKa = 4.24 EE192 pKa = 5.66 EE193 pKa = 4.57 ILTYY197 pKa = 10.67 DD198 pKa = 4.33 RR199 pKa = 11.84 EE200 pKa = 4.37 IEE202 pKa = 4.17 SMARR206 pKa = 11.84 RR207 pKa = 11.84 DD208 pKa = 3.54 EE209 pKa = 4.08 RR210 pKa = 11.84 AKK212 pKa = 10.82 RR213 pKa = 11.84 LMAIPGIGPQTATAILASAPDD234 pKa = 3.56 PRR236 pKa = 11.84 QFKK239 pKa = 10.21 SGRR242 pKa = 11.84 HH243 pKa = 3.6 FAAWLGLVPRR253 pKa = 11.84 QYY255 pKa = 10.49 STGGKK260 pKa = 8.48 PRR262 pKa = 11.84 LGRR265 pKa = 11.84 ITKK268 pKa = 10.47 RR269 pKa = 11.84 GDD271 pKa = 2.81 KK272 pKa = 10.35 YY273 pKa = 11.32 LRR275 pKa = 11.84 TLLIHH280 pKa = 5.89 GTRR283 pKa = 11.84 AVIAKK288 pKa = 9.85 LADD291 pKa = 3.48 KK292 pKa = 10.75 NDD294 pKa = 4.06 RR295 pKa = 11.84 LSGWARR301 pKa = 11.84 EE302 pKa = 4.02 LVEE305 pKa = 3.64 RR306 pKa = 11.84 RR307 pKa = 11.84 GYY309 pKa = 10.24 KK310 pKa = 9.6 RR311 pKa = 11.84 AAVALAAKK319 pKa = 9.37 NARR322 pKa = 11.84 IIWRR326 pKa = 11.84 YY327 pKa = 8.61
Molecular weight: 37.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.721
IPC_protein 10.643
Toseland 10.847
ProMoST 10.628
Dawson 10.921
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.067
Grimsley 10.965
Solomon 11.082
Lehninger 11.052
Nozaki 10.833
DTASelect 10.657
Thurlkill 10.833
EMBOSS 11.257
Sillero 10.862
Patrickios 10.774
IPC_peptide 11.096
IPC2_peptide 9.721
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3073
0
3073
917237
51
1623
298.5
32.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.817 ± 0.05
0.945 ± 0.013
5.521 ± 0.039
6.432 ± 0.047
3.859 ± 0.028
7.844 ± 0.039
2.18 ± 0.021
5.42 ± 0.037
4.023 ± 0.037
10.609 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.663 ± 0.023
3.42 ± 0.028
4.58 ± 0.032
3.999 ± 0.033
6.109 ± 0.033
6.167 ± 0.037
5.192 ± 0.027
7.262 ± 0.04
1.373 ± 0.022
2.585 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here