Amino acid dipepetide frequency for Marinobacter sp. AC-23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.773AlaAla: 9.773 ± 0.14	1.036AlaCys: 1.036 ± 0.033	5.291AlaAsp: 5.291 ± 0.091	6.407AlaGlu: 6.407 ± 0.096	3.825AlaPhe: 3.825 ± 0.077	8.304AlaGly: 8.304 ± 0.107	1.838AlaHis: 1.838 ± 0.04	5.704AlaIle: 5.704 ± 0.064	3.668AlaLys: 3.668 ± 0.065	11.217AlaLeu: 11.217 ± 0.137	3.008AlaMet: 3.008 ± 0.062	3.072AlaAsn: 3.072 ± 0.065	3.958AlaPro: 3.958 ± 0.071	3.653AlaGln: 3.653 ± 0.071	5.928AlaArg: 5.928 ± 0.089	6.058AlaSer: 6.058 ± 0.077	4.783AlaThr: 4.783 ± 0.079	6.994AlaVal: 6.994 ± 0.103	1.372AlaTrp: 1.372 ± 0.04	2.283AlaTyr: 2.283 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.873CysAla: 0.873 ± 0.03	0.137CysCys: 0.137 ± 0.012	0.551CysAsp: 0.551 ± 0.024	0.579CysGlu: 0.579 ± 0.027	0.361CysPhe: 0.361 ± 0.019	0.891CysGly: 0.891 ± 0.035	0.319CysHis: 0.319 ± 0.019	0.43CysIle: 0.43 ± 0.021	0.315CysLys: 0.315 ± 0.019	0.978CysLeu: 0.978 ± 0.03	0.223CysMet: 0.223 ± 0.016	0.252CysAsn: 0.252 ± 0.015	0.455CysPro: 0.455 ± 0.021	0.359CysGln: 0.359 ± 0.018	0.592CysArg: 0.592 ± 0.027	0.623CysSer: 0.623 ± 0.027	0.449CysThr: 0.449 ± 0.023	0.666CysVal: 0.666 ± 0.028	0.136CysTrp: 0.136 ± 0.013	0.257CysTyr: 0.257 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	4.979AspAla: 4.979 ± 0.072	0.451AspCys: 0.451 ± 0.021	3.139AspAsp: 3.139 ± 0.064	3.89AspGlu: 3.89 ± 0.067	2.154AspPhe: 2.154 ± 0.049	4.222AspGly: 4.222 ± 0.07	1.191AspHis: 1.191 ± 0.037	3.361AspIle: 3.361 ± 0.065	2.185AspLys: 2.185 ± 0.048	5.506AspLeu: 5.506 ± 0.079	1.552AspMet: 1.552 ± 0.043	1.996AspAsn: 1.996 ± 0.057	2.473AspPro: 2.473 ± 0.061	2.223AspGln: 2.223 ± 0.048	3.333AspArg: 3.333 ± 0.063	3.098AspSer: 3.098 ± 0.06	2.871AspThr: 2.871 ± 0.059	3.931AspVal: 3.931 ± 0.063	0.881AspTrp: 0.881 ± 0.034	1.769AspTyr: 1.769 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	6.503GluAla: 6.503 ± 0.099	0.509GluCys: 0.509 ± 0.026	3.263GluAsp: 3.263 ± 0.063	3.799GluGlu: 3.799 ± 0.079	2.222GluPhe: 2.222 ± 0.049	4.503GluGly: 4.503 ± 0.083	1.573GluHis: 1.573 ± 0.039	3.439GluIle: 3.439 ± 0.055	3.138GluLys: 3.138 ± 0.066	7.127GluLeu: 7.127 ± 0.102	1.609GluMet: 1.609 ± 0.044	2.396GluAsn: 2.396 ± 0.05	2.774GluPro: 2.774 ± 0.062	3.354GluGln: 3.354 ± 0.069	4.483GluArg: 4.483 ± 0.082	3.599GluSer: 3.599 ± 0.063	3.427GluThr: 3.427 ± 0.057	4.302GluVal: 4.302 ± 0.07	0.809GluTrp: 0.809 ± 0.031	1.497GluTyr: 1.497 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.453PheAla: 3.453 ± 0.062	0.425PheCys: 0.425 ± 0.021	2.477PheAsp: 2.477 ± 0.05	2.291PheGlu: 2.291 ± 0.052	1.696PhePhe: 1.696 ± 0.052	3.37PheGly: 3.37 ± 0.066	0.822PheHis: 0.822 ± 0.029	2.091PheIle: 2.091 ± 0.051	1.316PheLys: 1.316 ± 0.037	3.489PheLeu: 3.489 ± 0.067	0.951PheMet: 0.951 ± 0.034	1.422PheAsn: 1.422 ± 0.042	1.624PhePro: 1.624 ± 0.044	1.3PheGln: 1.3 ± 0.037	2.391PheArg: 2.391 ± 0.057	2.917PheSer: 2.917 ± 0.058	2.097PheThr: 2.097 ± 0.052	2.732PheVal: 2.732 ± 0.055	0.552PheTrp: 0.552 ± 0.028	1.174PheTyr: 1.174 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	6.806GlyAla: 6.806 ± 0.1	0.954GlyCys: 0.954 ± 0.037	4.267GlyAsp: 4.267 ± 0.08	5.124GlyGlu: 5.124 ± 0.074	3.506GlyPhe: 3.506 ± 0.068	5.997GlyGly: 5.997 ± 0.109	1.795GlyHis: 1.795 ± 0.045	4.755GlyIle: 4.755 ± 0.084	3.562GlyLys: 3.562 ± 0.064	8.475GlyLeu: 8.475 ± 0.106	2.404GlyMet: 2.404 ± 0.057	2.532GlyAsn: 2.532 ± 0.065	2.487GlyPro: 2.487 ± 0.055	3.185GlyGln: 3.185 ± 0.055	4.433GlyArg: 4.433 ± 0.074	4.675GlySer: 4.675 ± 0.075	3.901GlyThr: 3.901 ± 0.069	5.91GlyVal: 5.91 ± 0.097	1.224GlyTrp: 1.224 ± 0.039	2.448GlyTyr: 2.448 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.841HisAla: 1.841 ± 0.047	0.313HisCys: 0.313 ± 0.018	0.993HisAsp: 0.993 ± 0.035	1.257HisGlu: 1.257 ± 0.038	0.941HisPhe: 0.941 ± 0.037	1.797HisGly: 1.797 ± 0.05	0.654HisHis: 0.654 ± 0.026	1.113HisIle: 1.113 ± 0.035	0.797HisLys: 0.797 ± 0.032	2.21HisLeu: 2.21 ± 0.049	0.522HisMet: 0.522 ± 0.024	0.729HisAsn: 0.729 ± 0.028	1.367HisPro: 1.367 ± 0.041	0.954HisGln: 0.954 ± 0.032	1.391HisArg: 1.391 ± 0.043	1.333HisSer: 1.333 ± 0.038	1.04HisThr: 1.04 ± 0.033	1.362HisVal: 1.362 ± 0.038	0.396HisTrp: 0.396 ± 0.018	0.792HisTyr: 0.792 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.872IleAla: 5.872 ± 0.093	0.541IleCys: 0.541 ± 0.022	3.281IleAsp: 3.281 ± 0.051	3.717IleGlu: 3.717 ± 0.066	1.804IlePhe: 1.804 ± 0.054	4.479IleGly: 4.479 ± 0.075	1.213IleHis: 1.213 ± 0.037	2.728IleIle: 2.728 ± 0.066	2.071IleLys: 2.071 ± 0.046	4.931IleLeu: 4.931 ± 0.09	1.196IleMet: 1.196 ± 0.035	2.131IleAsn: 2.131 ± 0.048	2.611IlePro: 2.611 ± 0.057	1.801IleGln: 1.801 ± 0.049	3.578IleArg: 3.578 ± 0.067	3.499IleSer: 3.499 ± 0.063	3.122IleThr: 3.122 ± 0.059	3.701IleVal: 3.701 ± 0.067	0.601IleTrp: 0.601 ± 0.029	1.318IleTyr: 1.318 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.473LysAla: 4.473 ± 0.08	0.217LysCys: 0.217 ± 0.016	2.242LysAsp: 2.242 ± 0.051	2.453LysGlu: 2.453 ± 0.062	1.065LysPhe: 1.065 ± 0.036	2.839LysGly: 2.839 ± 0.062	1.007LysHis: 1.007 ± 0.031	1.924LysIle: 1.924 ± 0.043	1.953LysLys: 1.953 ± 0.056	4.195LysLeu: 4.195 ± 0.072	1.008LysMet: 1.008 ± 0.037	1.398LysAsn: 1.398 ± 0.038	2.21LysPro: 2.21 ± 0.053	1.753LysGln: 1.753 ± 0.045	2.56LysArg: 2.56 ± 0.053	2.332LysSer: 2.332 ± 0.053	2.355LysThr: 2.355 ± 0.049	2.971LysVal: 2.971 ± 0.059	0.448LysTrp: 0.448 ± 0.022	0.824LysTyr: 0.824 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	11.868LeuAla: 11.868 ± 0.127	1.035LeuCys: 1.035 ± 0.036	5.82LeuAsp: 5.82 ± 0.09	6.715LeuGlu: 6.715 ± 0.102	3.984LeuPhe: 3.984 ± 0.079	8.064LeuGly: 8.064 ± 0.104	1.964LeuHis: 1.964 ± 0.042	5.74LeuIle: 5.74 ± 0.102	4.615LeuLys: 4.615 ± 0.064	10.652LeuLeu: 10.652 ± 0.142	2.853LeuMet: 2.853 ± 0.058	3.78LeuAsn: 3.78 ± 0.066	5.217LeuPro: 5.217 ± 0.078	3.785LeuGln: 3.785 ± 0.07	6.017LeuArg: 6.017 ± 0.087	7.047LeuSer: 7.047 ± 0.08	5.814LeuThr: 5.814 ± 0.084	7.499LeuVal: 7.499 ± 0.105	1.257LeuTrp: 1.257 ± 0.042	2.363LeuTyr: 2.363 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	3.044MetAla: 3.044 ± 0.06	0.194MetCys: 0.194 ± 0.016	1.416MetAsp: 1.416 ± 0.038	1.476MetGlu: 1.476 ± 0.036	0.809MetPhe: 0.809 ± 0.037	2.063MetGly: 2.063 ± 0.045	0.533MetHis: 0.533 ± 0.024	1.351MetIle: 1.351 ± 0.039	1.283MetLys: 1.283 ± 0.04	2.828MetLeu: 2.828 ± 0.063	0.761MetMet: 0.761 ± 0.034	0.992MetAsn: 0.992 ± 0.033	1.409MetPro: 1.409 ± 0.043	0.991MetGln: 0.991 ± 0.036	1.413MetArg: 1.413 ± 0.031	1.929MetSer: 1.929 ± 0.049	1.623MetThr: 1.623 ± 0.039	1.878MetVal: 1.878 ± 0.046	0.177MetTrp: 0.177 ± 0.015	0.461MetTyr: 0.461 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.152AsnAla: 3.152 ± 0.058	0.302AsnCys: 0.302 ± 0.017	1.813AsnAsp: 1.813 ± 0.055	2.059AsnGlu: 2.059 ± 0.046	1.185AsnPhe: 1.185 ± 0.037	2.779AsnGly: 2.779 ± 0.064	0.772AsnHis: 0.772 ± 0.025	1.791AsnIle: 1.791 ± 0.044	1.241AsnLys: 1.241 ± 0.045	3.586AsnLeu: 3.586 ± 0.06	0.817AsnMet: 0.817 ± 0.029	1.338AsnAsn: 1.338 ± 0.043	2.085AsnPro: 2.085 ± 0.052	1.459AsnGln: 1.459 ± 0.039	2.308AsnArg: 2.308 ± 0.059	1.968AsnSer: 1.968 ± 0.052	1.857AsnThr: 1.857 ± 0.045	2.135AsnVal: 2.135 ± 0.055	0.563AsnTrp: 0.563 ± 0.024	0.989AsnTyr: 0.989 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.642ProAla: 4.642 ± 0.071	0.324ProCys: 0.324 ± 0.021	3.209ProAsp: 3.209 ± 0.058	4.374ProGlu: 4.374 ± 0.08	1.796ProPhe: 1.796 ± 0.048	3.902ProGly: 3.902 ± 0.072	0.875ProHis: 0.875 ± 0.032	2.115ProIle: 2.115 ± 0.051	1.753ProLys: 1.753 ± 0.053	4.527ProLeu: 4.527 ± 0.078	1.188ProMet: 1.188 ± 0.033	1.394ProAsn: 1.394 ± 0.043	1.694ProPro: 1.694 ± 0.046	1.6ProGln: 1.6 ± 0.048	2.04ProArg: 2.04 ± 0.047	2.532ProSer: 2.532 ± 0.052	2.032ProThr: 2.032 ± 0.041	4.068ProVal: 4.068 ± 0.07	0.649ProTrp: 0.649 ± 0.029	1.091ProTyr: 1.091 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.429GlnAla: 4.429 ± 0.072	0.353GlnCys: 0.353 ± 0.02	1.771GlnAsp: 1.771 ± 0.041	2.282GlnGlu: 2.282 ± 0.05	1.409GlnPhe: 1.409 ± 0.046	2.911GlnGly: 2.911 ± 0.049	0.898GlnHis: 0.898 ± 0.031	2.028GlnIle: 2.028 ± 0.045	1.738GlnLys: 1.738 ± 0.049	4.256GlnLeu: 4.256 ± 0.07	1.025GlnMet: 1.025 ± 0.036	1.285GlnAsn: 1.285 ± 0.039	1.96GlnPro: 1.96 ± 0.046	2.105GlnGln: 2.105 ± 0.058	2.653GlnArg: 2.653 ± 0.058	2.381GlnSer: 2.381 ± 0.055	1.934GlnThr: 1.934 ± 0.049	2.981GlnVal: 2.981 ± 0.053	0.642GlnTrp: 0.642 ± 0.028	0.954GlnTyr: 0.954 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	5.079ArgAla: 5.079 ± 0.076	0.543ArgCys: 0.543 ± 0.027	3.138ArgAsp: 3.138 ± 0.06	4.323ArgGlu: 4.323 ± 0.078	2.733ArgPhe: 2.733 ± 0.057	3.684ArgGly: 3.684 ± 0.059	1.484ArgHis: 1.484 ± 0.05	3.639ArgIle: 3.639 ± 0.066	2.838ArgLys: 2.838 ± 0.05	6.77ArgLeu: 6.77 ± 0.094	1.69ArgMet: 1.69 ± 0.041	2.177ArgAsn: 2.177 ± 0.044	2.534ArgPro: 2.534 ± 0.055	2.824ArgGln: 2.824 ± 0.056	3.99ArgArg: 3.99 ± 0.078	3.449ArgSer: 3.449 ± 0.063	2.876ArgThr: 2.876 ± 0.057	4.365ArgVal: 4.365 ± 0.069	0.955ArgTrp: 0.955 ± 0.035	1.994ArgTyr: 1.994 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	5.931SerAla: 5.931 ± 0.085	0.484SerCys: 0.484 ± 0.024	3.548SerAsp: 3.548 ± 0.077	3.884SerGlu: 3.884 ± 0.068	2.379SerPhe: 2.379 ± 0.047	5.764SerGly: 5.764 ± 0.087	1.353SerHis: 1.353 ± 0.038	3.088SerIle: 3.088 ± 0.062	2.237SerLys: 2.237 ± 0.054	6.534SerLeu: 6.534 ± 0.099	1.674SerMet: 1.674 ± 0.046	1.953SerAsn: 1.953 ± 0.044	2.731SerPro: 2.731 ± 0.056	2.387SerGln: 2.387 ± 0.06	3.842SerArg: 3.842 ± 0.062	3.886SerSer: 3.886 ± 0.087	2.965SerThr: 2.965 ± 0.059	4.656SerVal: 4.656 ± 0.071	0.842SerTrp: 0.842 ± 0.029	1.534SerTyr: 1.534 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.947ThrAla: 4.947 ± 0.079	0.47ThrCys: 0.47 ± 0.026	2.868ThrAsp: 2.868 ± 0.057	3.019ThrGlu: 3.019 ± 0.063	1.937ThrPhe: 1.937 ± 0.054	4.77ThrGly: 4.77 ± 0.081	1.11ThrHis: 1.11 ± 0.034	2.639ThrIle: 2.639 ± 0.059	1.521ThrLys: 1.521 ± 0.043	6.404ThrLeu: 6.404 ± 0.078	1.145ThrMet: 1.145 ± 0.033	1.572ThrAsn: 1.572 ± 0.054	2.957ThrPro: 2.957 ± 0.054	1.756ThrGln: 1.756 ± 0.047	3.098ThrArg: 3.098 ± 0.054	3.094ThrSer: 3.094 ± 0.067	2.733ThrThr: 2.733 ± 0.054	3.979ThrVal: 3.979 ± 0.077	0.664ThrTrp: 0.664 ± 0.027	1.239ThrTyr: 1.239 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	7.261ValAla: 7.261 ± 0.095	0.725ValCys: 0.725 ± 0.03	4.02ValAsp: 4.02 ± 0.066	4.456ValGlu: 4.456 ± 0.088	3.115ValPhe: 3.115 ± 0.061	5.097ValGly: 5.097 ± 0.09	1.431ValHis: 1.431 ± 0.042	4.385ValIle: 4.385 ± 0.07	2.655ValLys: 2.655 ± 0.061	7.496ValLeu: 7.496 ± 0.099	2.041ValMet: 2.041 ± 0.052	2.451ValAsn: 2.451 ± 0.047	3.326ValPro: 3.326 ± 0.056	2.378ValGln: 2.378 ± 0.057	4.225ValArg: 4.225 ± 0.068	4.852ValSer: 4.852 ± 0.076	4.113ValThr: 4.113 ± 0.069	5.894ValVal: 5.894 ± 0.101	0.915ValTrp: 0.915 ± 0.036	1.788ValTyr: 1.788 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	1.102TrpAla: 1.102 ± 0.037	0.149TrpCys: 0.149 ± 0.012	0.615TrpAsp: 0.615 ± 0.027	0.659TrpGlu: 0.659 ± 0.027	0.628TrpPhe: 0.628 ± 0.028	0.885TrpGly: 0.885 ± 0.031	0.395TrpHis: 0.395 ± 0.021	0.662TrpIle: 0.662 ± 0.025	0.487TrpLys: 0.487 ± 0.022	1.98TrpLeu: 1.98 ± 0.059	0.406TrpMet: 0.406 ± 0.023	0.433TrpAsn: 0.433 ± 0.024	0.637TrpPro: 0.637 ± 0.025	0.871TrpGln: 0.871 ± 0.033	0.887TrpArg: 0.887 ± 0.032	0.811TrpSer: 0.811 ± 0.032	0.59TrpThr: 0.59 ± 0.029	0.966TrpVal: 0.966 ± 0.037	0.227TrpTrp: 0.227 ± 0.02	0.346TrpTyr: 0.346 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.141TyrAla: 2.141 ± 0.052	0.283TyrCys: 0.283 ± 0.016	1.487TyrAsp: 1.487 ± 0.04	1.561TyrGlu: 1.561 ± 0.04	1.04TyrPhe: 1.04 ± 0.032	2.118TyrGly: 2.118 ± 0.056	0.575TyrHis: 0.575 ± 0.024	1.213TyrIle: 1.213 ± 0.034	0.856TyrLys: 0.856 ± 0.031	2.926TyrLeu: 2.926 ± 0.06	0.557TyrMet: 0.557 ± 0.026	0.894TyrAsn: 0.894 ± 0.027	1.297TyrPro: 1.297 ± 0.039	1.257TyrGln: 1.257 ± 0.036	1.924TyrArg: 1.924 ± 0.048	1.587TyrSer: 1.587 ± 0.042	1.341TyrThr: 1.341 ± 0.038	1.632TyrVal: 1.632 ± 0.041	0.427TyrTrp: 0.427 ± 0.022	0.728TyrTyr: 0.728 ± 0.029	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3073 proteins (917237 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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