Actinobaculum suis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group;

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K9ET46|A0A0K9ET46_9ACTO HAD family hydrolase OS=Actinobaculum suis OX=1657 GN=nagD_1 PE=3 SV=1
MM1 pKa = 7.14ATDD4 pKa = 3.65YY5 pKa = 10.33DD6 pKa = 3.7APRR9 pKa = 11.84NKK11 pKa = 10.59DD12 pKa = 2.83EE13 pKa = 4.24GLRR16 pKa = 11.84EE17 pKa = 3.91EE18 pKa = 5.16SLQQLTSQSDD28 pKa = 3.31QSSNVDD34 pKa = 3.19EE35 pKa = 5.54DD36 pKa = 3.69EE37 pKa = 4.45VEE39 pKa = 3.91AAEE42 pKa = 4.66GFEE45 pKa = 5.34LPGADD50 pKa = 3.82LSNVEE55 pKa = 4.47LSVNVIPAQDD65 pKa = 3.94DD66 pKa = 3.81EE67 pKa = 5.06FTCSVCFLVHH77 pKa = 6.54HH78 pKa = 7.0RR79 pKa = 11.84SQLDD83 pKa = 3.3HH84 pKa = 6.86MEE86 pKa = 5.38DD87 pKa = 3.72GLPVCMDD94 pKa = 3.96CAFF97 pKa = 4.05

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6ZBX4|A0A1G6ZBX4_9ACTO UDP-N-acetylmuramate--L-alanine ligase OS=Actinobaculum suis OX=1657 GN=murC PE=3 SV=1
MM1 pKa = 7.67AKK3 pKa = 9.67TGGTHH8 pKa = 7.67DD9 pKa = 3.19AATRR13 pKa = 11.84HH14 pKa = 6.01DD15 pKa = 4.08APTRR19 pKa = 11.84ATRR22 pKa = 11.84RR23 pKa = 11.84GSRR26 pKa = 11.84GATGQARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TPKK38 pKa = 10.31GKK40 pKa = 9.18PVTGATRR47 pKa = 11.84LPAEE51 pKa = 4.24NAEE54 pKa = 4.25KK55 pKa = 10.75VRR57 pKa = 11.84DD58 pKa = 3.96VMRR61 pKa = 11.84HH62 pKa = 5.15SIIMLAVATLVLTVLGLVMVFSAVTPGAMRR92 pKa = 11.84SAAAGATNTFRR103 pKa = 11.84GVYY106 pKa = 9.67LQIFYY111 pKa = 10.49ACVGIVVAAILSRR124 pKa = 11.84FPIEE128 pKa = 4.18FFRR131 pKa = 11.84KK132 pKa = 8.56SWAAFWAGALLLQCLVFSPLGVAVAGNRR160 pKa = 11.84NWVALGPIRR169 pKa = 11.84MQPSEE174 pKa = 4.23FLKK177 pKa = 10.78LATIIALAATLVNVRR192 pKa = 11.84GAASVNLLRR201 pKa = 11.84RR202 pKa = 11.84EE203 pKa = 3.68EE204 pKa = 4.11WEE206 pKa = 5.29DD207 pKa = 2.92IKK209 pKa = 11.59NWVWPIAIFLLSAGLVGLGHH229 pKa = 7.65DD230 pKa = 3.87MGTVLVFALFTAGMLWLAGMAWQWFAVGGLAGILGTALAIAVSLSRR276 pKa = 11.84LARR279 pKa = 11.84VQDD282 pKa = 3.58FFEE285 pKa = 5.53NIFTLPAGGSPTQADD300 pKa = 3.76YY301 pKa = 11.71ALWAFGSGGLGGSGLGTGIEE321 pKa = 4.08KK322 pKa = 10.29WPGNLAEE329 pKa = 4.7AQTDD333 pKa = 4.09FIFAVVGEE341 pKa = 4.16EE342 pKa = 5.24LGFLGCAAVVILFLMVGISLVRR364 pKa = 11.84ICIHH368 pKa = 5.96HH369 pKa = 6.97PSRR372 pKa = 11.84FAKK375 pKa = 9.33LTVGGIALWLCGQALANMLVVTSMLPVFGVPLPFMSQGGSSVISCLMAVGVAISAAFSVEE435 pKa = 3.76GVRR438 pKa = 11.84ASFKK442 pKa = 10.42QRR444 pKa = 11.84PHH446 pKa = 5.1LVKK449 pKa = 10.14RR450 pKa = 11.84VVSIVSKK457 pKa = 9.7TRR459 pKa = 3.02

Molecular weight: 48.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1723

0

1723

622842

30

3033

361.5

38.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.706 ± 0.087

0.69 ± 0.014

5.154 ± 0.054

6.609 ± 0.054

3.135 ± 0.032

8.641 ± 0.057

1.889 ± 0.025

5.011 ± 0.044

3.211 ± 0.044

9.015 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.027

2.911 ± 0.041

5.412 ± 0.048

3.632 ± 0.031

6.471 ± 0.065

5.802 ± 0.041

6.204 ± 0.047

7.691 ± 0.052

1.338 ± 0.023

2.464 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski