Syntrophus gentianae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Syntrophus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7Z959|A0A1H7Z959_9DELT Carbamate kinase OS=Syntrophus gentianae OX=43775 GN=SAMN04489760_12139 PE=3 SV=1
MM1 pKa = 6.84ITSAVSSSDD10 pKa = 3.06SSTTTVTNSNAYY22 pKa = 10.67DD23 pKa = 3.41MGTEE27 pKa = 4.18DD28 pKa = 5.83FLNLLLTEE36 pKa = 5.3LEE38 pKa = 4.55NQDD41 pKa = 3.39PMDD44 pKa = 4.24PVSTSEE50 pKa = 4.01MATQFATLTQVSQLNKK66 pKa = 9.09TNDD69 pKa = 3.44YY70 pKa = 11.32LEE72 pKa = 4.73TLSHH76 pKa = 5.6TQAVDD81 pKa = 3.49YY82 pKa = 10.62LGKK85 pKa = 8.73TISYY89 pKa = 9.03TGEE92 pKa = 4.09TNSDD96 pKa = 3.34GTSTEE101 pKa = 3.9KK102 pKa = 10.32TSLVTGVVYY111 pKa = 10.58KK112 pKa = 10.98DD113 pKa = 3.34SVPYY117 pKa = 11.18LMTKK121 pKa = 10.62DD122 pKa = 3.52GDD124 pKa = 3.97QVALSSITGVSSS136 pKa = 3.19

Molecular weight: 14.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7UQQ6|A0A1H7UQQ6_9DELT Uncharacterized protein OS=Syntrophus gentianae OX=43775 GN=SAMN04489760_10228 PE=4 SV=1
MM1 pKa = 7.01LTASALLLTAVTVARR16 pKa = 11.84IFRR19 pKa = 11.84HH20 pKa = 5.08KK21 pKa = 9.79VWWLKK26 pKa = 7.62GHH28 pKa = 6.8RR29 pKa = 11.84RR30 pKa = 11.84LALCGVAILAAGFLAAIVLISSSGQPHH57 pKa = 6.89FGSLHH62 pKa = 5.79PRR64 pKa = 11.84LGGLTLSGAVLTPVLGFSVFRR85 pKa = 11.84FPIRR89 pKa = 11.84AAAIRR94 pKa = 11.84RR95 pKa = 11.84IHH97 pKa = 6.15RR98 pKa = 11.84VSGRR102 pKa = 11.84LTAILTVITILSGLLLIGIFF122 pKa = 3.81

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3382

0

3382

1080969

25

2991

319.6

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.424 ± 0.044

1.216 ± 0.019

5.32 ± 0.026

6.906 ± 0.039

4.188 ± 0.028

7.714 ± 0.044

1.861 ± 0.018

6.593 ± 0.033

5.456 ± 0.04

10.315 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.019

3.542 ± 0.029

4.491 ± 0.029

3.215 ± 0.022

6.135 ± 0.035

6.23 ± 0.043

5.035 ± 0.029

6.729 ± 0.032

1.082 ± 0.017

3.017 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski