Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces; Talaromyces sect. Talaromyces; Talaromyces stipitatus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B8M480|B8M480_TALSN Uncharacterized protein OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=TSTA_040170 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.88STVATIAVSALLLGSQVAVANPIQKK27 pKa = 9.82RR28 pKa = 11.84DD29 pKa = 3.61IPSSFYY35 pKa = 11.27SNMAIDD41 pKa = 4.13GSIYY45 pKa = 10.78SFADD49 pKa = 3.31DD50 pKa = 4.46ASCNEE55 pKa = 3.63IFYY58 pKa = 9.22TAGSFAMASAVMSQFGASQNNEE80 pKa = 3.91LCGKK84 pKa = 8.48TIEE87 pKa = 4.12ITGANGVVQQAAIADD102 pKa = 3.94TAGADD107 pKa = 4.37IIDD110 pKa = 3.67MCLNLWEE117 pKa = 5.15DD118 pKa = 4.02FGGQDD123 pKa = 3.7GDD125 pKa = 4.04GTIMTGANGIQWRR138 pKa = 11.84LVV140 pKa = 3.2

Molecular weight:
14.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B8M613|B8M613_TALSN Uncharacterized protein OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=TSTA_023480 PE=4 SV=1
MM1 pKa = 6.78VTDD4 pKa = 4.1NPCFRR9 pKa = 11.84SAVVKK14 pKa = 8.96TYY16 pKa = 10.72RR17 pKa = 11.84SDD19 pKa = 3.3RR20 pKa = 11.84TGGIHH25 pKa = 6.16VPGSNRR31 pKa = 11.84EE32 pKa = 3.89FEE34 pKa = 4.33QEE36 pKa = 3.66VEE38 pKa = 4.08YY39 pKa = 10.83AIASTRR45 pKa = 11.84FSSRR49 pKa = 11.84SRR51 pKa = 11.84AGDD54 pKa = 3.26DD55 pKa = 3.18RR56 pKa = 11.84RR57 pKa = 11.84NGGGANNNAFRR68 pKa = 11.84THH70 pKa = 6.61NNTLNKK76 pKa = 9.79GPRR79 pKa = 11.84GLRR82 pKa = 11.84QRR84 pKa = 11.84QYY86 pKa = 8.79QHH88 pKa = 6.27CQNIQEE94 pKa = 4.47INDD97 pKa = 3.57LSGINSGKK105 pKa = 10.27KK106 pKa = 9.65SRR108 pKa = 3.99

Molecular weight:
12.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12468

553

13021

6264805

41

7896

481.1

53.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.02 ± 0.017

1.208 ± 0.008

5.606 ± 0.015

6.199 ± 0.02

3.755 ± 0.011

6.411 ± 0.02

2.428 ± 0.011

5.54 ± 0.014

4.973 ± 0.018

9.092 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.008

4.032 ± 0.01

5.606 ± 0.018

4.159 ± 0.014

6.033 ± 0.017

8.311 ± 0.025

6.127 ± 0.014

5.985 ± 0.014

1.463 ± 0.008

2.949 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski