Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum)
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13021 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8M480|B8M480_TALSN Uncharacterized protein OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=TSTA_040170 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 9.88 STVATIAVSALLLGSQVAVANPIQKK27 pKa = 9.82 RR28 pKa = 11.84 DD29 pKa = 3.61 IPSSFYY35 pKa = 11.27 SNMAIDD41 pKa = 4.13 GSIYY45 pKa = 10.78 SFADD49 pKa = 3.31 DD50 pKa = 4.46 ASCNEE55 pKa = 3.63 IFYY58 pKa = 9.22 TAGSFAMASAVMSQFGASQNNEE80 pKa = 3.91 LCGKK84 pKa = 8.48 TIEE87 pKa = 4.12 ITGANGVVQQAAIADD102 pKa = 3.94 TAGADD107 pKa = 4.37 IIDD110 pKa = 3.67 MCLNLWEE117 pKa = 5.15 DD118 pKa = 4.02 FGGQDD123 pKa = 3.7 GDD125 pKa = 4.04 GTIMTGANGIQWRR138 pKa = 11.84 LVV140 pKa = 3.2
Molecular weight: 14.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.541
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.897
Patrickios 1.901
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|B8M613|B8M613_TALSN Uncharacterized protein OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=TSTA_023480 PE=4 SV=1
MM1 pKa = 6.78 VTDD4 pKa = 4.1 NPCFRR9 pKa = 11.84 SAVVKK14 pKa = 8.96 TYY16 pKa = 10.72 RR17 pKa = 11.84 SDD19 pKa = 3.3 RR20 pKa = 11.84 TGGIHH25 pKa = 6.16 VPGSNRR31 pKa = 11.84 EE32 pKa = 3.89 FEE34 pKa = 4.33 QEE36 pKa = 3.66 VEE38 pKa = 4.08 YY39 pKa = 10.83 AIASTRR45 pKa = 11.84 FSSRR49 pKa = 11.84 SRR51 pKa = 11.84 AGDD54 pKa = 3.26 DD55 pKa = 3.18 RR56 pKa = 11.84 RR57 pKa = 11.84 NGGGANNNAFRR68 pKa = 11.84 THH70 pKa = 6.61 NNTLNKK76 pKa = 9.79 GPRR79 pKa = 11.84 GLRR82 pKa = 11.84 QRR84 pKa = 11.84 QYY86 pKa = 8.79 QHH88 pKa = 6.27 CQNIQEE94 pKa = 4.47 INDD97 pKa = 3.57 LSGINSGKK105 pKa = 10.27 KK106 pKa = 9.65 SRR108 pKa = 3.99
Molecular weight: 12.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.531
IPC_protein 10.379
Toseland 10.452
ProMoST 10.496
Dawson 10.599
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.716
Grimsley 10.657
Solomon 10.701
Lehninger 10.672
Nozaki 10.467
DTASelect 10.321
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.467
IPC_peptide 10.701
IPC2_peptide 9.487
IPC2.peptide.svr19 8.65
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12468
553
13021
6264805
41
7896
481.1
53.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.02 ± 0.017
1.208 ± 0.008
5.606 ± 0.015
6.199 ± 0.02
3.755 ± 0.011
6.411 ± 0.02
2.428 ± 0.011
5.54 ± 0.014
4.973 ± 0.018
9.092 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.102 ± 0.008
4.032 ± 0.01
5.606 ± 0.018
4.159 ± 0.014
6.033 ± 0.017
8.311 ± 0.025
6.127 ± 0.014
5.985 ± 0.014
1.463 ± 0.008
2.949 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here