Mycoplasma ovis str. Michigan

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma ovis

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9RBF1|V9RBF1_9MOLU Uncharacterized protein OS=Mycoplasma ovis str. Michigan OX=1415773 GN=OVS_00590 PE=4 SV=1
MM1 pKa = 7.09STLISLPEE9 pKa = 3.74FQIASSRR16 pKa = 11.84TLSSSVISSVSPRR29 pKa = 11.84LICAFIITQFPTISFTPGVIVAGVATSWAFNAGASEE65 pKa = 4.21IASSLVTGAEE75 pKa = 4.06RR76 pKa = 11.84EE77 pKa = 4.5SEE79 pKa = 4.42VTTDD83 pKa = 3.2TSEE86 pKa = 5.54DD87 pKa = 3.82GLSDD91 pKa = 3.11WTAWITGLPLEE102 pKa = 4.92EE103 pKa = 5.35PYY105 pKa = 10.9CCLSSQWIPAQPSPIPATKK124 pKa = 10.39ASALVLMGIANN135 pKa = 3.85

Molecular weight: 14.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9R8M1|V9R8M1_9MOLU Uncharacterized protein OS=Mycoplasma ovis str. Michigan OX=1415773 GN=OVS_00570 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.42KK9 pKa = 8.42RR10 pKa = 11.84KK11 pKa = 8.81RR12 pKa = 11.84LRR14 pKa = 11.84VHH16 pKa = 6.71GFLKK20 pKa = 10.52RR21 pKa = 11.84MSSRR25 pKa = 11.84TGRR28 pKa = 11.84SIVKK32 pKa = 9.79ARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.63GRR39 pKa = 11.84VRR41 pKa = 11.84LTVSSKK47 pKa = 11.05

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

834

0

834

193850

29

2646

232.4

26.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.044 ± 0.062

1.181 ± 0.034

4.558 ± 0.065

7.795 ± 0.108

5.275 ± 0.081

5.745 ± 0.085

1.384 ± 0.031

7.275 ± 0.086

10.15 ± 0.091

10.665 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.528 ± 0.029

5.706 ± 0.075

3.102 ± 0.053

3.779 ± 0.059

3.588 ± 0.058

9.076 ± 0.093

4.912 ± 0.071

5.201 ± 0.074

1.636 ± 0.044

3.401 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski