Pasivirus A1 isolate (isolate Swine/France/ Pasivirus 1/2010)
Average proteome isoelectric point is 5.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6YQK4|I6YQK4_PASI1 Capsid protein VP0 OS=Pasivirus A1 isolate (isolate Swine/France/Pasivirus 1/2010) OX=1554498 PE=4 SV=1
MM1 pKa = 7.7 DD2 pKa = 3.95 VVVDD6 pKa = 3.88 TLTKK10 pKa = 10.27 EE11 pKa = 4.31 VSQTLLGDD19 pKa = 3.63 TAEE22 pKa = 4.42 SGGGFGGVLSGSATTNAANIVQHH45 pKa = 6.09 SAAPTAPANPVLDD58 pKa = 4.93 ANDD61 pKa = 4.21 PYY63 pKa = 10.64 EE64 pKa = 4.2 ACAYY68 pKa = 10.3 SKK70 pKa = 8.62 EE71 pKa = 4.49 TSRR74 pKa = 11.84 EE75 pKa = 4.19 TPSKK79 pKa = 10.27 MVLLDD84 pKa = 3.3 IVHH87 pKa = 7.4 FDD89 pKa = 3.71 NTWVWGGNDD98 pKa = 3.73 KK99 pKa = 11.06 LQLPLPKK106 pKa = 10.19 CFWKK110 pKa = 10.57 NRR112 pKa = 11.84 SFPAYY117 pKa = 7.17 GTSRR121 pKa = 11.84 YY122 pKa = 8.93 FQFFRR127 pKa = 11.84 GSFHH131 pKa = 7.61 IKK133 pKa = 9.2 IQVNAAAGSAGSFVVVYY150 pKa = 9.59 VPNDD154 pKa = 3.44 HH155 pKa = 5.87 YY156 pKa = 11.46 QSWNNKK162 pKa = 9.0 DD163 pKa = 4.75 LILHH167 pKa = 6.18 TMFNWPHH174 pKa = 6.09 VIFNVATMTEE184 pKa = 3.83 ADD186 pKa = 4.47 LYY188 pKa = 10.37 IPYY191 pKa = 10.2 INTHH195 pKa = 5.63 NLANTDD201 pKa = 3.39 SSDD204 pKa = 3.86 CGWVVVTPFTHH215 pKa = 7.32 LEE217 pKa = 4.2 VASTTTNTLDD227 pKa = 3.16 IAVFGSLVDD236 pKa = 4.17 FQFQGPRR243 pKa = 11.84 PYY245 pKa = 10.53 TDD247 pKa = 4.64 LPSDD251 pKa = 3.54 SQGPKK256 pKa = 9.3 RR257 pKa = 11.84 VARR260 pKa = 11.84 NKK262 pKa = 9.59 KK263 pKa = 9.73 YY264 pKa = 10.49 KK265 pKa = 8.17 YY266 pKa = 8.35 TRR268 pKa = 11.84 NKK270 pKa = 9.53 IDD272 pKa = 3.15 IAEE275 pKa = 4.83 GPGSMNIANVLSTTAAQSIALVGEE299 pKa = 4.18 RR300 pKa = 11.84 AFIDD304 pKa = 3.8 PSVAGTRR311 pKa = 11.84 VRR313 pKa = 11.84 VRR315 pKa = 11.84 TFRR318 pKa = 11.84 DD319 pKa = 3.32 VTKK322 pKa = 10.72 IKK324 pKa = 10.57 ALPLLPFTASHH335 pKa = 6.24 SEE337 pKa = 3.87 YY338 pKa = 10.85 CIFDD342 pKa = 3.6 WQASHH347 pKa = 7.14 APTTKK352 pKa = 10.36 SPIFGRR358 pKa = 11.84 GYY360 pKa = 10.55 KK361 pKa = 9.56 IRR363 pKa = 11.84 EE364 pKa = 4.21 LGNLGPLSTAYY375 pKa = 10.18 QFWRR379 pKa = 11.84 GSIIFTLTIFASSFHH394 pKa = 6.86 RR395 pKa = 11.84 GRR397 pKa = 11.84 LRR399 pKa = 11.84 IVLFPNGTHH408 pKa = 6.87 SFSQDD413 pKa = 3.06 DD414 pKa = 3.9 ANALIFTVCDD424 pKa = 3.01 IGEE427 pKa = 4.05 QSSFDD432 pKa = 2.95 ITLPFTHH439 pKa = 6.39 YY440 pKa = 10.59 NWIRR444 pKa = 11.84 EE445 pKa = 4.23 CDD447 pKa = 3.4 NSVVVLVNVYY457 pKa = 10.23 VEE459 pKa = 3.96 TRR461 pKa = 11.84 LAHH464 pKa = 6.44 NNASSNVIKK473 pKa = 10.62 CALFVRR479 pKa = 11.84 GGEE482 pKa = 4.06 DD483 pKa = 3.7 FEE485 pKa = 5.39 FLAPCGDD492 pKa = 3.53 TVKK495 pKa = 10.51 FHH497 pKa = 6.41 GWGSDD502 pKa = 3.07 MDD504 pKa = 4.21 EE505 pKa = 5.06 ADD507 pKa = 4.59 PLDD510 pKa = 4.33 DD511 pKa = 4.87 MEE513 pKa = 5.85 PGHH516 pKa = 7.07 PDD518 pKa = 3.51 CLTLEE523 pKa = 4.48 SKK525 pKa = 10.75 DD526 pKa = 3.56 GSSGKK531 pKa = 10.41 GPQCAQMAGLTSVEE545 pKa = 4.02 NDD547 pKa = 3.03 GTTDD551 pKa = 3.34 NQLNCAAPKK560 pKa = 10.04 FINIHH565 pKa = 6.22 KK566 pKa = 8.43 LTTSIFAKK574 pKa = 10.62 DD575 pKa = 3.97 HH576 pKa = 5.25 MHH578 pKa = 7.1 LDD580 pKa = 3.8 YY581 pKa = 11.14 FFGRR585 pKa = 11.84 GWYY588 pKa = 9.14 VMKK591 pKa = 10.57 HH592 pKa = 4.79 NYY594 pKa = 9.25 EE595 pKa = 4.3 GEE597 pKa = 4.2 SRR599 pKa = 11.84 APFYY603 pKa = 10.77 IDD605 pKa = 2.84 VDD607 pKa = 4.22 APSSMPGAAMNFFSFWAGEE626 pKa = 4.36 VNFHH630 pKa = 5.95 VVNNSDD636 pKa = 3.94 DD637 pKa = 3.85 PMAVTHH643 pKa = 6.63 SYY645 pKa = 9.91 SHH647 pKa = 6.44 EE648 pKa = 4.04 ASTVGPAEE656 pKa = 3.98 GLGLIIVPPRR666 pKa = 11.84 EE667 pKa = 4.37 MISFAAPFYY676 pKa = 10.13 SWSPLRR682 pKa = 11.84 TVLAHH687 pKa = 5.87 QNSAVKK693 pKa = 9.35 RR694 pKa = 11.84 TFGILKK700 pKa = 9.36 LVPEE704 pKa = 4.46 APKK707 pKa = 10.81 GQVIVYY713 pKa = 9.82 ASLRR717 pKa = 11.84 TPCFFLVKK725 pKa = 9.62 PFPIQTTAAKK735 pKa = 9.12 MAVYY739 pKa = 9.68 PSKK742 pKa = 10.26 LTSAEE747 pKa = 3.81 EE748 pKa = 4.0 AEE750 pKa = 4.13 EE751 pKa = 4.39 LKK753 pKa = 10.82 FKK755 pKa = 10.61 LVHH758 pKa = 6.16 EE759 pKa = 4.85 PYY761 pKa = 9.51 EE762 pKa = 4.13 QFFIPHH768 pKa = 6.34 YY769 pKa = 10.71 GDD771 pKa = 3.18 IPSFQRR777 pKa = 11.84 DD778 pKa = 4.24 FINWLGSKK786 pKa = 10.59 EE787 pKa = 3.99 EE788 pKa = 4.14 LQNMILQCGDD798 pKa = 3.24 VEE800 pKa = 4.5 QNPGPVLMYY809 pKa = 10.65 DD810 pKa = 3.74 GNKK813 pKa = 9.33 KK814 pKa = 10.31 FCVPVCNGYY823 pKa = 8.18 LTFHH827 pKa = 7.07 GSPEE831 pKa = 3.87 QFLVSGNGTLIIYY844 pKa = 10.2 KK845 pKa = 9.35 EE846 pKa = 4.35 VYY848 pKa = 10.62 GMLPYY853 pKa = 10.8 EE854 pKa = 3.95 EE855 pKa = 5.87 AYY857 pKa = 10.1 PEE859 pKa = 4.38 DD860 pKa = 5.26 LSSNWHH866 pKa = 6.72 IEE868 pKa = 4.05 WQCVNGKK875 pKa = 9.53 VDD877 pKa = 3.8 SLVKK881 pKa = 10.58 RR882 pKa = 11.84 MANWMLLRR890 pKa = 11.84 YY891 pKa = 9.04 IDD893 pKa = 4.21 LVIFGGTWRR902 pKa = 11.84 RR903 pKa = 11.84 LLAPNTDD910 pKa = 3.22 MKK912 pKa = 10.87 FDD914 pKa = 3.79 SQGVDD919 pKa = 3.19 DD920 pKa = 5.13 MMNSLTGLLNSSAMEE935 pKa = 3.97 LLGNDD940 pKa = 3.09 VSKK943 pKa = 11.16 SILCCLVRR951 pKa = 11.84 FVAVLVMFFRR961 pKa = 11.84 NPDD964 pKa = 4.26 LINGVALASYY974 pKa = 9.47 LASEE978 pKa = 4.95 FSSVRR983 pKa = 11.84 LVSSQTAAFVDD994 pKa = 3.71 ALFNGDD1000 pKa = 4.16 LYY1002 pKa = 11.42 KK1003 pKa = 10.91 LSEE1006 pKa = 4.32 QFLSMMDD1013 pKa = 3.17 IGSDD1017 pKa = 3.24 SDD1019 pKa = 3.77 YY1020 pKa = 11.16 KK1021 pKa = 10.98 QLIKK1025 pKa = 9.91 EE1026 pKa = 4.53 TLGSYY1031 pKa = 9.42 PPEE1034 pKa = 4.04 MNPFEE1039 pKa = 4.46 SQSLAAFNALSLSARR1054 pKa = 11.84 NCTWWIEE1061 pKa = 4.02 LFQKK1065 pKa = 10.58 LHH1067 pKa = 5.52 TFVRR1071 pKa = 11.84 DD1072 pKa = 3.28 LLKK1075 pKa = 10.43 PDD1077 pKa = 3.78 TSAKK1081 pKa = 8.79 FHH1083 pKa = 5.33 EE1084 pKa = 4.61 WCRR1087 pKa = 11.84 KK1088 pKa = 9.03 NKK1090 pKa = 9.24 MLISNFMYY1098 pKa = 10.24 TLNEE1102 pKa = 4.02 HH1103 pKa = 6.81 LKK1105 pKa = 10.94 DD1106 pKa = 4.24 CATTCNLRR1114 pKa = 11.84 KK1115 pKa = 9.74 RR1116 pKa = 11.84 EE1117 pKa = 3.86 FRR1119 pKa = 11.84 DD1120 pKa = 3.03 KK1121 pKa = 11.03 HH1122 pKa = 5.36 KK1123 pKa = 10.59 WLLSVLNRR1131 pKa = 11.84 LTEE1134 pKa = 3.95 GFTTYY1139 pKa = 10.2 GVQNDD1144 pKa = 3.41 ITRR1147 pKa = 11.84 ALLDD1151 pKa = 3.41 MRR1153 pKa = 11.84 KK1154 pKa = 10.33 SMLAVKK1160 pKa = 10.42 LGQEE1164 pKa = 4.2 YY1165 pKa = 10.88 QNTCARR1171 pKa = 11.84 EE1172 pKa = 3.94 EE1173 pKa = 4.03 PLGVLLRR1180 pKa = 11.84 GEE1182 pKa = 4.35 PGQGKK1187 pKa = 9.65 SFMTHH1192 pKa = 5.9 LLVRR1196 pKa = 11.84 SVCNAMGWKK1205 pKa = 9.89 VSEE1208 pKa = 4.7 SVFSHH1213 pKa = 5.83 PTSSEE1218 pKa = 3.73 FFDD1221 pKa = 5.62 GYY1223 pKa = 10.95 AGQPIHH1229 pKa = 7.06 LIDD1232 pKa = 5.35 DD1233 pKa = 4.8 FGQNVEE1239 pKa = 4.06 EE1240 pKa = 4.48 PEE1242 pKa = 4.22 YY1243 pKa = 10.99 KK1244 pKa = 10.46 VLCQCMSSIPFSVPMASLEE1263 pKa = 4.36 EE1264 pKa = 4.15 KK1265 pKa = 10.41 GLPYY1269 pKa = 10.04 TSKK1272 pKa = 11.0 LVIATTNKK1280 pKa = 10.02 SDD1282 pKa = 2.88 WSSRR1286 pKa = 11.84 TLCAPGALKK1295 pKa = 10.45 RR1296 pKa = 11.84 RR1297 pKa = 11.84 FPIVYY1302 pKa = 8.18 TVRR1305 pKa = 11.84 ARR1307 pKa = 11.84 RR1308 pKa = 11.84 TLTKK1312 pKa = 10.76 NGLLDD1317 pKa = 3.85 VNAHH1321 pKa = 5.47 MSAVRR1326 pKa = 11.84 DD1327 pKa = 3.8 GAAWEE1332 pKa = 4.33 YY1333 pKa = 6.98 TTNGYY1338 pKa = 9.76 DD1339 pKa = 3.28 WQPFSVNDD1347 pKa = 3.89 LAAEE1351 pKa = 4.51 IIKK1354 pKa = 10.24 QLEE1357 pKa = 3.79 QRR1359 pKa = 11.84 RR1360 pKa = 11.84 EE1361 pKa = 4.11 SFQLWNSFISQAPEE1375 pKa = 3.78 TFEE1378 pKa = 4.25 AWCDD1382 pKa = 3.69 DD1383 pKa = 3.66 MVSLLGPAVPVASVFDD1399 pKa = 4.07 YY1400 pKa = 10.9 CASTGVVPMLKK1411 pKa = 9.65 QASEE1415 pKa = 4.54 SVLKK1419 pKa = 10.6 WFDD1422 pKa = 3.36 SCVDD1426 pKa = 3.47 SMVSFVTRR1434 pKa = 11.84 NKK1436 pKa = 10.14 KK1437 pKa = 9.1 WIAFGALTMSVLGIAISMLKK1457 pKa = 10.18 EE1458 pKa = 3.94 LDD1460 pKa = 3.27 SQRR1463 pKa = 11.84 PYY1465 pKa = 10.96 NQTAHH1470 pKa = 6.9 KK1471 pKa = 9.99 MPVTKK1476 pKa = 9.62 LTRR1479 pKa = 11.84 GRR1481 pKa = 11.84 RR1482 pKa = 11.84 VLVSQGPYY1490 pKa = 10.6 NEE1492 pKa = 4.04 FQHH1495 pKa = 5.73 YY1496 pKa = 10.37 SKK1498 pKa = 11.34 YY1499 pKa = 10.65 CVFLHH1504 pKa = 6.36 SGSITLHH1511 pKa = 5.64 GLAFGGNSFLFYY1523 pKa = 10.16 THH1525 pKa = 6.9 GLATLEE1531 pKa = 5.26 RR1532 pKa = 11.84 YY1533 pKa = 10.02 DD1534 pKa = 4.44 NWVLDD1539 pKa = 4.29 YY1540 pKa = 10.75 NGSMFDD1546 pKa = 4.4 LDD1548 pKa = 3.96 IEE1550 pKa = 4.78 TVDD1553 pKa = 3.53 EE1554 pKa = 4.88 LYY1556 pKa = 11.33 LNGEE1560 pKa = 4.2 SMDD1563 pKa = 4.1 LCLITCKK1570 pKa = 10.16 PLPVTFASIVGHH1582 pKa = 7.13 LSDD1585 pKa = 5.1 GDD1587 pKa = 3.98 LGDD1590 pKa = 5.75 GVILWRR1596 pKa = 11.84 GRR1598 pKa = 11.84 EE1599 pKa = 4.16 GLTCMPIYY1607 pKa = 10.18 DD1608 pKa = 3.86 LHH1610 pKa = 8.34 HH1611 pKa = 6.77 YY1612 pKa = 7.88 GTVTTNQGDD1621 pKa = 3.97 TCTSAIRR1628 pKa = 11.84 YY1629 pKa = 6.95 RR1630 pKa = 11.84 AKK1632 pKa = 8.38 TTRR1635 pKa = 11.84 GMCGGLVLSKK1645 pKa = 10.74 ISGTYY1650 pKa = 9.29 KK1651 pKa = 10.64 AVGLHH1656 pKa = 4.77 VAGNGVYY1663 pKa = 10.25 GVAASLSVCKK1673 pKa = 10.22 QLEE1676 pKa = 4.41 SQGLVTDD1683 pKa = 4.12 TKK1685 pKa = 8.44 PWPGVRR1691 pKa = 11.84 VHH1693 pKa = 6.05 QPSKK1697 pKa = 11.04 SVLKK1701 pKa = 9.86 PSPLYY1706 pKa = 10.99 GFVEE1710 pKa = 4.49 QEE1712 pKa = 3.66 LHH1714 pKa = 6.28 PAVLSPFDD1722 pKa = 3.83 NRR1724 pKa = 11.84 LKK1726 pKa = 11.09 CEE1728 pKa = 3.76 VDD1730 pKa = 3.7 SVIGRR1735 pKa = 11.84 QALKK1739 pKa = 10.81 YY1740 pKa = 9.83 NSNVFNPGPYY1750 pKa = 9.14 YY1751 pKa = 10.8 DD1752 pKa = 4.17 EE1753 pKa = 4.22 VLEE1756 pKa = 4.33 AFAVDD1761 pKa = 3.65 FNGRR1765 pKa = 11.84 FGINEE1770 pKa = 3.85 MMSAEE1775 pKa = 4.19 EE1776 pKa = 4.16 VFALSGDD1783 pKa = 3.82 EE1784 pKa = 4.88 AIEE1787 pKa = 4.41 LSTSPGLKK1795 pKa = 8.87 YY1796 pKa = 9.27 TSKK1799 pKa = 11.11 GLRR1802 pKa = 11.84 KK1803 pKa = 9.23 RR1804 pKa = 11.84 DD1805 pKa = 3.6 LVPGGKK1811 pKa = 9.77 ACDD1814 pKa = 3.91 LLLSDD1819 pKa = 5.62 VEE1821 pKa = 4.98 RR1822 pKa = 11.84 LMNDD1826 pKa = 3.23 PKK1828 pKa = 10.3 GTPVYY1833 pKa = 9.37 FYY1835 pKa = 11.3 CHH1837 pKa = 6.94 LKK1839 pKa = 10.98 DD1840 pKa = 3.55 EE1841 pKa = 5.01 LRR1843 pKa = 11.84 PQEE1846 pKa = 4.99 KK1847 pKa = 9.55 IEE1849 pKa = 4.03 QGLTRR1854 pKa = 11.84 CIEE1857 pKa = 4.12 SSDD1860 pKa = 3.29 FDD1862 pKa = 4.12 YY1863 pKa = 11.44 VFSAKK1868 pKa = 9.85 RR1869 pKa = 11.84 VFCKK1873 pKa = 10.49 LFRR1876 pKa = 11.84 QLYY1879 pKa = 9.91 DD1880 pKa = 3.22 SDD1882 pKa = 4.78 PIEE1885 pKa = 4.06 TGFAVGINCIADD1897 pKa = 3.58 WKK1899 pKa = 10.76 NLLDD1903 pKa = 4.16 GMYY1906 pKa = 10.31 DD1907 pKa = 3.48 YY1908 pKa = 10.72 IYY1910 pKa = 11.11 DD1911 pKa = 4.21 FDD1913 pKa = 4.73 LKK1915 pKa = 10.84 GYY1917 pKa = 10.05 DD1918 pKa = 3.52 GSIPRR1923 pKa = 11.84 QLMIDD1928 pKa = 3.85 ACRR1931 pKa = 11.84 VLSEE1935 pKa = 4.29 SCSDD1939 pKa = 3.62 EE1940 pKa = 3.76 EE1941 pKa = 4.28 LAYY1944 pKa = 11.01 NLLHH1948 pKa = 6.16 KK1949 pKa = 9.41 TVDD1952 pKa = 3.6 SQHH1955 pKa = 5.9 VVGNQLLTVSGGMASGSPFTTIVNTACNILANMTVLEE1992 pKa = 4.37 EE1993 pKa = 4.16 EE1994 pKa = 4.53 GVEE1997 pKa = 4.12 YY1998 pKa = 10.45 TLVCYY2003 pKa = 10.63 GDD2005 pKa = 5.46 DD2006 pKa = 3.57 ILVSTSDD2013 pKa = 4.17 PISVNDD2019 pKa = 3.81 YY2020 pKa = 9.06 VDD2022 pKa = 4.63 KK2023 pKa = 10.4 MGQYY2027 pKa = 10.58 FGFTVKK2033 pKa = 10.66 LDD2035 pKa = 3.22 GDD2037 pKa = 3.99 YY2038 pKa = 11.19 PKK2040 pKa = 10.4 TKK2042 pKa = 11.0 NNFTFLKK2049 pKa = 10.49 RR2050 pKa = 11.84 RR2051 pKa = 11.84 TTYY2054 pKa = 10.06 WNGIPVGAMEE2064 pKa = 4.96 VGLLLQHH2071 pKa = 6.99 LGYY2074 pKa = 9.94 CRR2076 pKa = 11.84 SVSVFQDD2083 pKa = 3.33 QLNSASIEE2091 pKa = 4.16 LALHH2095 pKa = 6.23 GPEE2098 pKa = 4.23 TYY2100 pKa = 10.24 NVLRR2104 pKa = 11.84 SRR2106 pKa = 11.84 VNSFLNGTGFALMSYY2121 pKa = 9.45 RR2122 pKa = 11.84 RR2123 pKa = 11.84 AAAVVGSMLSS2133 pKa = 3.2
Molecular weight: 237.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.411
IPC2_protein 5.423
IPC_protein 5.474
Toseland 5.753
ProMoST 5.766
Dawson 5.664
Bjellqvist 5.703
Wikipedia 5.639
Rodwell 5.626
Grimsley 5.855
Solomon 5.664
Lehninger 5.652
Nozaki 5.893
DTASelect 6.084
Thurlkill 6.02
EMBOSS 5.982
Sillero 5.982
Patrickios 3.719
IPC_peptide 5.677
IPC2_peptide 5.995
IPC2.peptide.svr19 5.923
Protein with the highest isoelectric point:
>tr|I6YQK4|I6YQK4_PASI1 Capsid protein VP0 OS=Pasivirus A1 isolate (isolate Swine/France/Pasivirus 1/2010) OX=1554498 PE=4 SV=1
MM1 pKa = 7.7 DD2 pKa = 3.95 VVVDD6 pKa = 3.88 TLTKK10 pKa = 10.27 EE11 pKa = 4.31 VSQTLLGDD19 pKa = 3.63 TAEE22 pKa = 4.42 SGGGFGGVLSGSATTNAANIVQHH45 pKa = 6.09 SAAPTAPANPVLDD58 pKa = 4.93 ANDD61 pKa = 4.21 PYY63 pKa = 10.64 EE64 pKa = 4.2 ACAYY68 pKa = 10.3 SKK70 pKa = 8.62 EE71 pKa = 4.49 TSRR74 pKa = 11.84 EE75 pKa = 4.19 TPSKK79 pKa = 10.27 MVLLDD84 pKa = 3.3 IVHH87 pKa = 7.4 FDD89 pKa = 3.71 NTWVWGGNDD98 pKa = 3.73 KK99 pKa = 11.06 LQLPLPKK106 pKa = 10.19 CFWKK110 pKa = 10.57 NRR112 pKa = 11.84 SFPAYY117 pKa = 7.17 GTSRR121 pKa = 11.84 YY122 pKa = 8.93 FQFFRR127 pKa = 11.84 GSFHH131 pKa = 7.61 IKK133 pKa = 9.2 IQVNAAAGSAGSFVVVYY150 pKa = 9.59 VPNDD154 pKa = 3.44 HH155 pKa = 5.87 YY156 pKa = 11.46 QSWNNKK162 pKa = 9.0 DD163 pKa = 4.75 LILHH167 pKa = 6.18 TMFNWPHH174 pKa = 6.09 VIFNVATMTEE184 pKa = 3.83 ADD186 pKa = 4.47 LYY188 pKa = 10.37 IPYY191 pKa = 10.2 INTHH195 pKa = 5.63 NLANTDD201 pKa = 3.39 SSDD204 pKa = 3.86 CGWVVVTPFTHH215 pKa = 7.32 LEE217 pKa = 4.2 VASTTTNTLDD227 pKa = 3.16 IAVFGSLVDD236 pKa = 4.17 FQFQGPRR243 pKa = 11.84 PYY245 pKa = 10.53 TDD247 pKa = 4.64 LPSDD251 pKa = 3.54 SQGPKK256 pKa = 9.3 RR257 pKa = 11.84 VARR260 pKa = 11.84 NKK262 pKa = 9.59 KK263 pKa = 9.73 YY264 pKa = 10.49 KK265 pKa = 8.17 YY266 pKa = 8.35 TRR268 pKa = 11.84 NKK270 pKa = 9.53 IDD272 pKa = 3.15 IAEE275 pKa = 4.83 GPGSMNIANVLSTTAAQSIALVGEE299 pKa = 4.18 RR300 pKa = 11.84 AFIDD304 pKa = 3.8 PSVAGTRR311 pKa = 11.84 VRR313 pKa = 11.84 VRR315 pKa = 11.84 TFRR318 pKa = 11.84 DD319 pKa = 3.32 VTKK322 pKa = 10.72 IKK324 pKa = 10.57 ALPLLPFTASHH335 pKa = 6.24 SEE337 pKa = 3.87 YY338 pKa = 10.85 CIFDD342 pKa = 3.6 WQASHH347 pKa = 7.14 APTTKK352 pKa = 10.36 SPIFGRR358 pKa = 11.84 GYY360 pKa = 10.55 KK361 pKa = 9.56 IRR363 pKa = 11.84 EE364 pKa = 4.21 LGNLGPLSTAYY375 pKa = 10.18 QFWRR379 pKa = 11.84 GSIIFTLTIFASSFHH394 pKa = 6.86 RR395 pKa = 11.84 GRR397 pKa = 11.84 LRR399 pKa = 11.84 IVLFPNGTHH408 pKa = 6.87 SFSQDD413 pKa = 3.06 DD414 pKa = 3.9 ANALIFTVCDD424 pKa = 3.01 IGEE427 pKa = 4.05 QSSFDD432 pKa = 2.95 ITLPFTHH439 pKa = 6.39 YY440 pKa = 10.59 NWIRR444 pKa = 11.84 EE445 pKa = 4.23 CDD447 pKa = 3.4 NSVVVLVNVYY457 pKa = 10.23 VEE459 pKa = 3.96 TRR461 pKa = 11.84 LAHH464 pKa = 6.44 NNASSNVIKK473 pKa = 10.62 CALFVRR479 pKa = 11.84 GGEE482 pKa = 4.06 DD483 pKa = 3.7 FEE485 pKa = 5.39 FLAPCGDD492 pKa = 3.53 TVKK495 pKa = 10.51 FHH497 pKa = 6.41 GWGSDD502 pKa = 3.07 MDD504 pKa = 4.21 EE505 pKa = 5.06 ADD507 pKa = 4.59 PLDD510 pKa = 4.33 DD511 pKa = 4.87 MEE513 pKa = 5.85 PGHH516 pKa = 7.07 PDD518 pKa = 3.51 CLTLEE523 pKa = 4.48 SKK525 pKa = 10.75 DD526 pKa = 3.56 GSSGKK531 pKa = 10.41 GPQCAQMAGLTSVEE545 pKa = 4.02 NDD547 pKa = 3.03 GTTDD551 pKa = 3.34 NQLNCAAPKK560 pKa = 10.04 FINIHH565 pKa = 6.22 KK566 pKa = 8.43 LTTSIFAKK574 pKa = 10.62 DD575 pKa = 3.97 HH576 pKa = 5.25 MHH578 pKa = 7.1 LDD580 pKa = 3.8 YY581 pKa = 11.14 FFGRR585 pKa = 11.84 GWYY588 pKa = 9.14 VMKK591 pKa = 10.57 HH592 pKa = 4.79 NYY594 pKa = 9.25 EE595 pKa = 4.3 GEE597 pKa = 4.2 SRR599 pKa = 11.84 APFYY603 pKa = 10.77 IDD605 pKa = 2.84 VDD607 pKa = 4.22 APSSMPGAAMNFFSFWAGEE626 pKa = 4.36 VNFHH630 pKa = 5.95 VVNNSDD636 pKa = 3.94 DD637 pKa = 3.85 PMAVTHH643 pKa = 6.63 SYY645 pKa = 9.91 SHH647 pKa = 6.44 EE648 pKa = 4.04 ASTVGPAEE656 pKa = 3.98 GLGLIIVPPRR666 pKa = 11.84 EE667 pKa = 4.37 MISFAAPFYY676 pKa = 10.13 SWSPLRR682 pKa = 11.84 TVLAHH687 pKa = 5.87 QNSAVKK693 pKa = 9.35 RR694 pKa = 11.84 TFGILKK700 pKa = 9.36 LVPEE704 pKa = 4.46 APKK707 pKa = 10.81 GQVIVYY713 pKa = 9.82 ASLRR717 pKa = 11.84 TPCFFLVKK725 pKa = 9.62 PFPIQTTAAKK735 pKa = 9.12 MAVYY739 pKa = 9.68 PSKK742 pKa = 10.26 LTSAEE747 pKa = 3.81 EE748 pKa = 4.0 AEE750 pKa = 4.13 EE751 pKa = 4.39 LKK753 pKa = 10.82 FKK755 pKa = 10.61 LVHH758 pKa = 6.16 EE759 pKa = 4.85 PYY761 pKa = 9.51 EE762 pKa = 4.13 QFFIPHH768 pKa = 6.34 YY769 pKa = 10.71 GDD771 pKa = 3.18 IPSFQRR777 pKa = 11.84 DD778 pKa = 4.24 FINWLGSKK786 pKa = 10.59 EE787 pKa = 3.99 EE788 pKa = 4.14 LQNMILQCGDD798 pKa = 3.24 VEE800 pKa = 4.5 QNPGPVLMYY809 pKa = 10.65 DD810 pKa = 3.74 GNKK813 pKa = 9.33 KK814 pKa = 10.31 FCVPVCNGYY823 pKa = 8.18 LTFHH827 pKa = 7.07 GSPEE831 pKa = 3.87 QFLVSGNGTLIIYY844 pKa = 10.2 KK845 pKa = 9.35 EE846 pKa = 4.35 VYY848 pKa = 10.62 GMLPYY853 pKa = 10.8 EE854 pKa = 3.95 EE855 pKa = 5.87 AYY857 pKa = 10.1 PEE859 pKa = 4.38 DD860 pKa = 5.26 LSSNWHH866 pKa = 6.72 IEE868 pKa = 4.05 WQCVNGKK875 pKa = 9.53 VDD877 pKa = 3.8 SLVKK881 pKa = 10.58 RR882 pKa = 11.84 MANWMLLRR890 pKa = 11.84 YY891 pKa = 9.04 IDD893 pKa = 4.21 LVIFGGTWRR902 pKa = 11.84 RR903 pKa = 11.84 LLAPNTDD910 pKa = 3.22 MKK912 pKa = 10.87 FDD914 pKa = 3.79 SQGVDD919 pKa = 3.19 DD920 pKa = 5.13 MMNSLTGLLNSSAMEE935 pKa = 3.97 LLGNDD940 pKa = 3.09 VSKK943 pKa = 11.16 SILCCLVRR951 pKa = 11.84 FVAVLVMFFRR961 pKa = 11.84 NPDD964 pKa = 4.26 LINGVALASYY974 pKa = 9.47 LASEE978 pKa = 4.95 FSSVRR983 pKa = 11.84 LVSSQTAAFVDD994 pKa = 3.71 ALFNGDD1000 pKa = 4.16 LYY1002 pKa = 11.42 KK1003 pKa = 10.91 LSEE1006 pKa = 4.32 QFLSMMDD1013 pKa = 3.17 IGSDD1017 pKa = 3.24 SDD1019 pKa = 3.77 YY1020 pKa = 11.16 KK1021 pKa = 10.98 QLIKK1025 pKa = 9.91 EE1026 pKa = 4.53 TLGSYY1031 pKa = 9.42 PPEE1034 pKa = 4.04 MNPFEE1039 pKa = 4.46 SQSLAAFNALSLSARR1054 pKa = 11.84 NCTWWIEE1061 pKa = 4.02 LFQKK1065 pKa = 10.58 LHH1067 pKa = 5.52 TFVRR1071 pKa = 11.84 DD1072 pKa = 3.28 LLKK1075 pKa = 10.43 PDD1077 pKa = 3.78 TSAKK1081 pKa = 8.79 FHH1083 pKa = 5.33 EE1084 pKa = 4.61 WCRR1087 pKa = 11.84 KK1088 pKa = 9.03 NKK1090 pKa = 9.24 MLISNFMYY1098 pKa = 10.24 TLNEE1102 pKa = 4.02 HH1103 pKa = 6.81 LKK1105 pKa = 10.94 DD1106 pKa = 4.24 CATTCNLRR1114 pKa = 11.84 KK1115 pKa = 9.74 RR1116 pKa = 11.84 EE1117 pKa = 3.86 FRR1119 pKa = 11.84 DD1120 pKa = 3.03 KK1121 pKa = 11.03 HH1122 pKa = 5.36 KK1123 pKa = 10.59 WLLSVLNRR1131 pKa = 11.84 LTEE1134 pKa = 3.95 GFTTYY1139 pKa = 10.2 GVQNDD1144 pKa = 3.41 ITRR1147 pKa = 11.84 ALLDD1151 pKa = 3.41 MRR1153 pKa = 11.84 KK1154 pKa = 10.33 SMLAVKK1160 pKa = 10.42 LGQEE1164 pKa = 4.2 YY1165 pKa = 10.88 QNTCARR1171 pKa = 11.84 EE1172 pKa = 3.94 EE1173 pKa = 4.03 PLGVLLRR1180 pKa = 11.84 GEE1182 pKa = 4.35 PGQGKK1187 pKa = 9.65 SFMTHH1192 pKa = 5.9 LLVRR1196 pKa = 11.84 SVCNAMGWKK1205 pKa = 9.89 VSEE1208 pKa = 4.7 SVFSHH1213 pKa = 5.83 PTSSEE1218 pKa = 3.73 FFDD1221 pKa = 5.62 GYY1223 pKa = 10.95 AGQPIHH1229 pKa = 7.06 LIDD1232 pKa = 5.35 DD1233 pKa = 4.8 FGQNVEE1239 pKa = 4.06 EE1240 pKa = 4.48 PEE1242 pKa = 4.22 YY1243 pKa = 10.99 KK1244 pKa = 10.46 VLCQCMSSIPFSVPMASLEE1263 pKa = 4.36 EE1264 pKa = 4.15 KK1265 pKa = 10.41 GLPYY1269 pKa = 10.04 TSKK1272 pKa = 11.0 LVIATTNKK1280 pKa = 10.02 SDD1282 pKa = 2.88 WSSRR1286 pKa = 11.84 TLCAPGALKK1295 pKa = 10.45 RR1296 pKa = 11.84 RR1297 pKa = 11.84 FPIVYY1302 pKa = 8.18 TVRR1305 pKa = 11.84 ARR1307 pKa = 11.84 RR1308 pKa = 11.84 TLTKK1312 pKa = 10.76 NGLLDD1317 pKa = 3.85 VNAHH1321 pKa = 5.47 MSAVRR1326 pKa = 11.84 DD1327 pKa = 3.8 GAAWEE1332 pKa = 4.33 YY1333 pKa = 6.98 TTNGYY1338 pKa = 9.76 DD1339 pKa = 3.28 WQPFSVNDD1347 pKa = 3.89 LAAEE1351 pKa = 4.51 IIKK1354 pKa = 10.24 QLEE1357 pKa = 3.79 QRR1359 pKa = 11.84 RR1360 pKa = 11.84 EE1361 pKa = 4.11 SFQLWNSFISQAPEE1375 pKa = 3.78 TFEE1378 pKa = 4.25 AWCDD1382 pKa = 3.69 DD1383 pKa = 3.66 MVSLLGPAVPVASVFDD1399 pKa = 4.07 YY1400 pKa = 10.9 CASTGVVPMLKK1411 pKa = 9.65 QASEE1415 pKa = 4.54 SVLKK1419 pKa = 10.6 WFDD1422 pKa = 3.36 SCVDD1426 pKa = 3.47 SMVSFVTRR1434 pKa = 11.84 NKK1436 pKa = 10.14 KK1437 pKa = 9.1 WIAFGALTMSVLGIAISMLKK1457 pKa = 10.18 EE1458 pKa = 3.94 LDD1460 pKa = 3.27 SQRR1463 pKa = 11.84 PYY1465 pKa = 10.96 NQTAHH1470 pKa = 6.9 KK1471 pKa = 9.99 MPVTKK1476 pKa = 9.62 LTRR1479 pKa = 11.84 GRR1481 pKa = 11.84 RR1482 pKa = 11.84 VLVSQGPYY1490 pKa = 10.6 NEE1492 pKa = 4.04 FQHH1495 pKa = 5.73 YY1496 pKa = 10.37 SKK1498 pKa = 11.34 YY1499 pKa = 10.65 CVFLHH1504 pKa = 6.36 SGSITLHH1511 pKa = 5.64 GLAFGGNSFLFYY1523 pKa = 10.16 THH1525 pKa = 6.9 GLATLEE1531 pKa = 5.26 RR1532 pKa = 11.84 YY1533 pKa = 10.02 DD1534 pKa = 4.44 NWVLDD1539 pKa = 4.29 YY1540 pKa = 10.75 NGSMFDD1546 pKa = 4.4 LDD1548 pKa = 3.96 IEE1550 pKa = 4.78 TVDD1553 pKa = 3.53 EE1554 pKa = 4.88 LYY1556 pKa = 11.33 LNGEE1560 pKa = 4.2 SMDD1563 pKa = 4.1 LCLITCKK1570 pKa = 10.16 PLPVTFASIVGHH1582 pKa = 7.13 LSDD1585 pKa = 5.1 GDD1587 pKa = 3.98 LGDD1590 pKa = 5.75 GVILWRR1596 pKa = 11.84 GRR1598 pKa = 11.84 EE1599 pKa = 4.16 GLTCMPIYY1607 pKa = 10.18 DD1608 pKa = 3.86 LHH1610 pKa = 8.34 HH1611 pKa = 6.77 YY1612 pKa = 7.88 GTVTTNQGDD1621 pKa = 3.97 TCTSAIRR1628 pKa = 11.84 YY1629 pKa = 6.95 RR1630 pKa = 11.84 AKK1632 pKa = 8.38 TTRR1635 pKa = 11.84 GMCGGLVLSKK1645 pKa = 10.74 ISGTYY1650 pKa = 9.29 KK1651 pKa = 10.64 AVGLHH1656 pKa = 4.77 VAGNGVYY1663 pKa = 10.25 GVAASLSVCKK1673 pKa = 10.22 QLEE1676 pKa = 4.41 SQGLVTDD1683 pKa = 4.12 TKK1685 pKa = 8.44 PWPGVRR1691 pKa = 11.84 VHH1693 pKa = 6.05 QPSKK1697 pKa = 11.04 SVLKK1701 pKa = 9.86 PSPLYY1706 pKa = 10.99 GFVEE1710 pKa = 4.49 QEE1712 pKa = 3.66 LHH1714 pKa = 6.28 PAVLSPFDD1722 pKa = 3.83 NRR1724 pKa = 11.84 LKK1726 pKa = 11.09 CEE1728 pKa = 3.76 VDD1730 pKa = 3.7 SVIGRR1735 pKa = 11.84 QALKK1739 pKa = 10.81 YY1740 pKa = 9.83 NSNVFNPGPYY1750 pKa = 9.14 YY1751 pKa = 10.8 DD1752 pKa = 4.17 EE1753 pKa = 4.22 VLEE1756 pKa = 4.33 AFAVDD1761 pKa = 3.65 FNGRR1765 pKa = 11.84 FGINEE1770 pKa = 3.85 MMSAEE1775 pKa = 4.19 EE1776 pKa = 4.16 VFALSGDD1783 pKa = 3.82 EE1784 pKa = 4.88 AIEE1787 pKa = 4.41 LSTSPGLKK1795 pKa = 8.87 YY1796 pKa = 9.27 TSKK1799 pKa = 11.11 GLRR1802 pKa = 11.84 KK1803 pKa = 9.23 RR1804 pKa = 11.84 DD1805 pKa = 3.6 LVPGGKK1811 pKa = 9.77 ACDD1814 pKa = 3.91 LLLSDD1819 pKa = 5.62 VEE1821 pKa = 4.98 RR1822 pKa = 11.84 LMNDD1826 pKa = 3.23 PKK1828 pKa = 10.3 GTPVYY1833 pKa = 9.37 FYY1835 pKa = 11.3 CHH1837 pKa = 6.94 LKK1839 pKa = 10.98 DD1840 pKa = 3.55 EE1841 pKa = 5.01 LRR1843 pKa = 11.84 PQEE1846 pKa = 4.99 KK1847 pKa = 9.55 IEE1849 pKa = 4.03 QGLTRR1854 pKa = 11.84 CIEE1857 pKa = 4.12 SSDD1860 pKa = 3.29 FDD1862 pKa = 4.12 YY1863 pKa = 11.44 VFSAKK1868 pKa = 9.85 RR1869 pKa = 11.84 VFCKK1873 pKa = 10.49 LFRR1876 pKa = 11.84 QLYY1879 pKa = 9.91 DD1880 pKa = 3.22 SDD1882 pKa = 4.78 PIEE1885 pKa = 4.06 TGFAVGINCIADD1897 pKa = 3.58 WKK1899 pKa = 10.76 NLLDD1903 pKa = 4.16 GMYY1906 pKa = 10.31 DD1907 pKa = 3.48 YY1908 pKa = 10.72 IYY1910 pKa = 11.11 DD1911 pKa = 4.21 FDD1913 pKa = 4.73 LKK1915 pKa = 10.84 GYY1917 pKa = 10.05 DD1918 pKa = 3.52 GSIPRR1923 pKa = 11.84 QLMIDD1928 pKa = 3.85 ACRR1931 pKa = 11.84 VLSEE1935 pKa = 4.29 SCSDD1939 pKa = 3.62 EE1940 pKa = 3.76 EE1941 pKa = 4.28 LAYY1944 pKa = 11.01 NLLHH1948 pKa = 6.16 KK1949 pKa = 9.41 TVDD1952 pKa = 3.6 SQHH1955 pKa = 5.9 VVGNQLLTVSGGMASGSPFTTIVNTACNILANMTVLEE1992 pKa = 4.37 EE1993 pKa = 4.16 EE1994 pKa = 4.53 GVEE1997 pKa = 4.12 YY1998 pKa = 10.45 TLVCYY2003 pKa = 10.63 GDD2005 pKa = 5.46 DD2006 pKa = 3.57 ILVSTSDD2013 pKa = 4.17 PISVNDD2019 pKa = 3.81 YY2020 pKa = 9.06 VDD2022 pKa = 4.63 KK2023 pKa = 10.4 MGQYY2027 pKa = 10.58 FGFTVKK2033 pKa = 10.66 LDD2035 pKa = 3.22 GDD2037 pKa = 3.99 YY2038 pKa = 11.19 PKK2040 pKa = 10.4 TKK2042 pKa = 11.0 NNFTFLKK2049 pKa = 10.49 RR2050 pKa = 11.84 RR2051 pKa = 11.84 TTYY2054 pKa = 10.06 WNGIPVGAMEE2064 pKa = 4.96 VGLLLQHH2071 pKa = 6.99 LGYY2074 pKa = 9.94 CRR2076 pKa = 11.84 SVSVFQDD2083 pKa = 3.33 QLNSASIEE2091 pKa = 4.16 LALHH2095 pKa = 6.23 GPEE2098 pKa = 4.23 TYY2100 pKa = 10.24 NVLRR2104 pKa = 11.84 SRR2106 pKa = 11.84 VNSFLNGTGFALMSYY2121 pKa = 9.45 RR2122 pKa = 11.84 RR2123 pKa = 11.84 AAAVVGSMLSS2133 pKa = 3.2
Molecular weight: 237.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.411
IPC2_protein 5.423
IPC_protein 5.474
Toseland 5.753
ProMoST 5.766
Dawson 5.664
Bjellqvist 5.703
Wikipedia 5.639
Rodwell 5.626
Grimsley 5.855
Solomon 5.664
Lehninger 5.652
Nozaki 5.893
DTASelect 6.084
Thurlkill 6.02
EMBOSS 5.982
Sillero 5.982
Patrickios 3.719
IPC_peptide 5.677
IPC2_peptide 5.995
IPC2.peptide.svr19 5.923
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
2133
2133
2133
2133.0
237.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.61 ± 0.0
2.25 ± 0.0
5.813 ± 0.0
4.923 ± 0.0
5.485 ± 0.0
7.079 ± 0.0
2.438 ± 0.0
4.173 ± 0.0
4.641 ± 0.0
9.611 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.0
4.923 ± 0.0
4.829 ± 0.0
3.094 ± 0.0
4.126 ± 0.0
8.158 ± 0.0
6.001 ± 0.0
7.595 ± 0.0
1.641 ± 0.0
3.891 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here