Marinobacterium lutimaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinobacterium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5UWV7|A0A1H5UWV7_9GAMM Transglutaminase-like enzyme putative cysteine protease OS=Marinobacterium lutimaris OX=568106 GN=SAMN05444390_101544 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.32KK3 pKa = 10.35SLIALAVAGAMTAPVVAQADD23 pKa = 3.53ATLYY27 pKa = 11.08GNVEE31 pKa = 3.95IEE33 pKa = 4.3GVFANDD39 pKa = 3.2ADD41 pKa = 5.02AEE43 pKa = 4.31IQVDD47 pKa = 3.88DD48 pKa = 4.19ARR50 pKa = 11.84MGVKK54 pKa = 10.47GSDD57 pKa = 2.9EE58 pKa = 4.31TYY60 pKa = 10.27IDD62 pKa = 4.19GVSSFYY68 pKa = 10.76QIEE71 pKa = 4.44LEE73 pKa = 4.19YY74 pKa = 11.32NPDD77 pKa = 3.51SVLSDD82 pKa = 3.55GNSVTVRR89 pKa = 11.84KK90 pKa = 9.94ASAGLTGGFGTVIGGRR106 pKa = 11.84FSNPVEE112 pKa = 4.22STEE115 pKa = 5.02LNDD118 pKa = 4.9LYY120 pKa = 11.25SEE122 pKa = 4.26SLSTDD127 pKa = 3.94FFFRR131 pKa = 11.84DD132 pKa = 3.45PDD134 pKa = 4.25RR135 pKa = 11.84IGSALAYY142 pKa = 7.33ITPTFGGFSAYY153 pKa = 10.4AGIAADD159 pKa = 4.08GNADD163 pKa = 3.42RR164 pKa = 11.84HH165 pKa = 5.86ALNGADD171 pKa = 4.83NDD173 pKa = 4.25RR174 pKa = 11.84EE175 pKa = 4.15DD176 pKa = 4.23LDD178 pKa = 4.68GYY180 pKa = 11.18LVGADD185 pKa = 3.49YY186 pKa = 10.13TIGGFGAHH194 pKa = 6.53LGYY197 pKa = 10.47WSMDD201 pKa = 3.02KK202 pKa = 11.3DD203 pKa = 3.96GDD205 pKa = 4.19SEE207 pKa = 4.42AAGAAHH213 pKa = 6.81DD214 pKa = 4.11AEE216 pKa = 4.94YY217 pKa = 10.48IGLALSYY224 pKa = 10.38AVSNFTFTGSYY235 pKa = 11.04AEE237 pKa = 4.59ADD239 pKa = 3.79SVDD242 pKa = 3.32GGFIAGTVNTEE253 pKa = 3.59LWTLAADD260 pKa = 3.75YY261 pKa = 11.65AMEE264 pKa = 4.19NTNFGISYY272 pKa = 9.4MDD274 pKa = 3.4YY275 pKa = 10.99QEE277 pKa = 4.29SQKK280 pKa = 11.46SNDD283 pKa = 2.75IDD285 pKa = 4.0ADD287 pKa = 3.32EE288 pKa = 4.32WGVYY292 pKa = 9.34VSHH295 pKa = 7.13KK296 pKa = 10.25LSNKK300 pKa = 9.87ASVKK304 pKa = 10.14AQYY307 pKa = 10.12TSADD311 pKa = 3.25VDD313 pKa = 3.46NTDD316 pKa = 3.46IVGEE320 pKa = 4.13DD321 pKa = 3.75VFVVGYY327 pKa = 9.93NVSFF331 pKa = 4.04

Molecular weight: 35.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6DHK0|A0A1H6DHK0_9GAMM Uncharacterized protein OS=Marinobacterium lutimaris OX=568106 GN=SAMN05444390_106107 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5061

0

5061

1652987

27

10227

326.6

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.048 ± 0.044

1.012 ± 0.015

5.676 ± 0.047

6.616 ± 0.035

3.686 ± 0.023

7.771 ± 0.05

2.085 ± 0.02

5.452 ± 0.026

3.653 ± 0.032

11.18 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.023

3.266 ± 0.024

4.506 ± 0.029

4.247 ± 0.024

6.016 ± 0.04

6.333 ± 0.032

4.984 ± 0.039

6.943 ± 0.032

1.316 ± 0.015

2.678 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski