Pseudomonas phage Bf7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bifseptvirus; Pseudomonas virus Bf7

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2ELU8|H2ELU8_9CAUD Uncharacterized protein OS=Pseudomonas phage Bf7 OX=1100790 GN=BF7_00060 PE=4 SV=1
MM1 pKa = 7.85AEE3 pKa = 3.88ACAFACDD10 pKa = 4.12ALAHH14 pKa = 6.71ADD16 pKa = 3.63PLEE19 pKa = 4.45VVLEE23 pKa = 4.04PTPEE27 pKa = 4.27DD28 pKa = 3.25LLYY31 pKa = 10.86RR32 pKa = 11.84EE33 pKa = 5.15GFDD36 pKa = 4.19AYY38 pKa = 9.83TGVDD42 pKa = 3.37TSLSGAHH49 pKa = 6.09EE50 pKa = 4.36SAMLEE55 pKa = 4.08YY56 pKa = 10.82GFALLDD62 pKa = 4.8DD63 pKa = 4.66EE64 pKa = 5.59DD65 pKa = 3.87AA66 pKa = 5.24

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2ELU5|H2ELU5_9CAUD Uncharacterized protein OS=Pseudomonas phage Bf7 OX=1100790 GN=BF7_00045 PE=4 SV=1
MM1 pKa = 7.49SRR3 pKa = 11.84ALKK6 pKa = 10.69AGDD9 pKa = 3.79LVRR12 pKa = 11.84VSLPSARR19 pKa = 11.84SAYY22 pKa = 10.33ARR24 pKa = 11.84GLPFGAVVEE33 pKa = 4.4LEE35 pKa = 4.31GEE37 pKa = 4.21QVQYY41 pKa = 11.68GEE43 pKa = 3.91LHH45 pKa = 6.18GPLFRR50 pKa = 11.84IKK52 pKa = 10.68AQGNIPAYY60 pKa = 9.4LLPVSGLKK68 pKa = 9.66LAKK71 pKa = 9.24QAMRR75 pKa = 11.84KK76 pKa = 9.2RR77 pKa = 11.84EE78 pKa = 4.0SYY80 pKa = 11.05ANRR83 pKa = 11.84RR84 pKa = 11.84GG85 pKa = 3.29

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12533

48

1240

272.5

29.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.577 ± 0.673

0.838 ± 0.14

6.248 ± 0.246

5.45 ± 0.284

3.63 ± 0.168

7.923 ± 0.284

2.051 ± 0.194

4.564 ± 0.206

5.21 ± 0.295

8.218 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.912 ± 0.198

3.981 ± 0.304

4.476 ± 0.289

4.907 ± 0.396

5.745 ± 0.344

5.529 ± 0.226

5.641 ± 0.339

6.79 ± 0.321

1.476 ± 0.121

2.833 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski