Amino acid dipepetide frequency for Pseudomonas phage Bf7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.715AlaAla: 17.715 ± 2.151
0.798AlaCys: 0.798 ± 0.294
6.783AlaAsp: 6.783 ± 0.683
7.421AlaGlu: 7.421 ± 0.767
3.351AlaPhe: 3.351 ± 0.561
9.496AlaGly: 9.496 ± 0.885
2.154AlaHis: 2.154 ± 0.421
5.825AlaIle: 5.825 ± 0.712
5.905AlaLys: 5.905 ± 0.803
9.735AlaLeu: 9.735 ± 0.91
3.431AlaMet: 3.431 ± 0.541
5.346AlaAsn: 5.346 ± 1.32
2.873AlaPro: 2.873 ± 0.501
5.027AlaGln: 5.027 ± 0.73
6.304AlaArg: 6.304 ± 0.707
5.107AlaSer: 5.107 ± 0.507
5.426AlaThr: 5.426 ± 1.231
8.139AlaVal: 8.139 ± 0.866
1.596AlaTrp: 1.596 ± 0.35
3.351AlaTyr: 3.351 ± 0.513
0.0AlaXaa: 0.0 ± 0.0
Cys
0.878CysAla: 0.878 ± 0.211
0.08CysCys: 0.08 ± 0.077
0.798CysAsp: 0.798 ± 0.247
0.239CysGlu: 0.239 ± 0.129
0.16CysPhe: 0.16 ± 0.112
0.239CysGly: 0.239 ± 0.17
0.479CysHis: 0.479 ± 0.164
0.239CysIle: 0.239 ± 0.143
0.718CysLys: 0.718 ± 0.273
0.798CysLeu: 0.798 ± 0.295
0.319CysMet: 0.319 ± 0.172
0.399CysAsn: 0.399 ± 0.231
0.479CysPro: 0.479 ± 0.234
0.16CysGln: 0.16 ± 0.105
0.638CysArg: 0.638 ± 0.258
0.559CysSer: 0.559 ± 0.19
0.559CysThr: 0.559 ± 0.229
0.479CysVal: 0.479 ± 0.178
0.08CysTrp: 0.08 ± 0.076
0.08CysTyr: 0.08 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
7.022AspAla: 7.022 ± 0.708
0.718AspCys: 0.718 ± 0.255
3.671AspAsp: 3.671 ± 0.455
3.032AspGlu: 3.032 ± 0.518
2.154AspPhe: 2.154 ± 0.41
5.187AspGly: 5.187 ± 0.7
0.878AspHis: 0.878 ± 0.312
3.032AspIle: 3.032 ± 0.481
3.272AspLys: 3.272 ± 0.505
5.825AspLeu: 5.825 ± 0.667
2.154AspMet: 2.154 ± 0.443
1.915AspAsn: 1.915 ± 0.351
3.91AspPro: 3.91 ± 0.473
2.713AspGln: 2.713 ± 0.571
3.112AspArg: 3.112 ± 0.399
3.75AspSer: 3.75 ± 0.599
2.713AspThr: 2.713 ± 0.446
4.868AspVal: 4.868 ± 0.641
0.718AspTrp: 0.718 ± 0.231
1.835AspTyr: 1.835 ± 0.3
0.0AspXaa: 0.0 ± 0.0
Glu
5.985GluAla: 5.985 ± 0.723
0.559GluCys: 0.559 ± 0.179
2.793GluAsp: 2.793 ± 0.483
1.995GluGlu: 1.995 ± 0.406
2.234GluPhe: 2.234 ± 0.467
4.628GluGly: 4.628 ± 0.594
1.516GluHis: 1.516 ± 0.418
2.234GluIle: 2.234 ± 0.454
2.314GluLys: 2.314 ± 0.396
5.745GluLeu: 5.745 ± 0.653
1.197GluMet: 1.197 ± 0.261
1.357GluAsn: 1.357 ± 0.31
2.793GluPro: 2.793 ± 0.42
3.032GluGln: 3.032 ± 0.597
3.112GluArg: 3.112 ± 0.537
3.272GluSer: 3.272 ± 0.475
1.995GluThr: 1.995 ± 0.289
4.389GluVal: 4.389 ± 0.696
1.436GluTrp: 1.436 ± 0.414
1.915GluTyr: 1.915 ± 0.383
0.0GluXaa: 0.0 ± 0.0
Phe
2.553PheAla: 2.553 ± 0.528
0.239PheCys: 0.239 ± 0.131
3.351PheAsp: 3.351 ± 0.52
1.835PheGlu: 1.835 ± 0.36
1.037PhePhe: 1.037 ± 0.268
3.192PheGly: 3.192 ± 0.482
0.958PheHis: 0.958 ± 0.268
1.915PheIle: 1.915 ± 0.322
2.633PheLys: 2.633 ± 0.5
2.234PheLeu: 2.234 ± 0.319
0.958PheMet: 0.958 ± 0.217
1.995PheAsn: 1.995 ± 0.403
1.516PhePro: 1.516 ± 0.368
1.835PheGln: 1.835 ± 0.296
2.314PheArg: 2.314 ± 0.427
2.154PheSer: 2.154 ± 0.443
1.995PheThr: 1.995 ± 0.367
2.314PheVal: 2.314 ± 0.353
0.319PheTrp: 0.319 ± 0.164
0.958PheTyr: 0.958 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
8.219GlyAla: 8.219 ± 0.988
0.878GlyCys: 0.878 ± 0.329
5.426GlyAsp: 5.426 ± 0.546
3.431GlyGlu: 3.431 ± 0.491
2.713GlyPhe: 2.713 ± 0.36
7.581GlyGly: 7.581 ± 0.839
1.357GlyHis: 1.357 ± 0.295
3.112GlyIle: 3.112 ± 0.368
6.304GlyLys: 6.304 ± 0.706
4.628GlyLeu: 4.628 ± 0.643
3.591GlyMet: 3.591 ± 0.684
3.671GlyAsn: 3.671 ± 0.629
2.553GlyPro: 2.553 ± 0.365
4.229GlyGln: 4.229 ± 0.718
4.389GlyArg: 4.389 ± 0.547
5.187GlySer: 5.187 ± 0.668
4.389GlyThr: 4.389 ± 0.705
4.947GlyVal: 4.947 ± 0.506
0.878GlyTrp: 0.878 ± 0.191
1.756GlyTyr: 1.756 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
2.394HisAla: 2.394 ± 0.438
0.319HisCys: 0.319 ± 0.15
1.117HisAsp: 1.117 ± 0.305
0.798HisGlu: 0.798 ± 0.228
0.958HisPhe: 0.958 ± 0.32
1.676HisGly: 1.676 ± 0.37
0.559HisHis: 0.559 ± 0.317
1.197HisIle: 1.197 ± 0.273
0.798HisLys: 0.798 ± 0.26
1.516HisLeu: 1.516 ± 0.338
0.479HisMet: 0.479 ± 0.21
0.878HisAsn: 0.878 ± 0.277
1.436HisPro: 1.436 ± 0.356
0.559HisGln: 0.559 ± 0.171
1.277HisArg: 1.277 ± 0.388
1.117HisSer: 1.117 ± 0.341
1.277HisThr: 1.277 ± 0.293
1.277HisVal: 1.277 ± 0.292
0.559HisTrp: 0.559 ± 0.189
0.319HisTyr: 0.319 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
4.788IleAla: 4.788 ± 0.674
0.559IleCys: 0.559 ± 0.181
2.633IleAsp: 2.633 ± 0.462
3.192IleGlu: 3.192 ± 0.4
1.516IlePhe: 1.516 ± 0.385
3.75IleGly: 3.75 ± 0.724
0.638IleHis: 0.638 ± 0.226
1.915IleIle: 1.915 ± 0.4
2.952IleLys: 2.952 ± 0.426
3.671IleLeu: 3.671 ± 0.548
1.516IleMet: 1.516 ± 0.261
2.474IleAsn: 2.474 ± 0.419
2.474IlePro: 2.474 ± 0.453
2.474IleGln: 2.474 ± 0.434
2.873IleArg: 2.873 ± 0.51
2.154IleSer: 2.154 ± 0.338
2.793IleThr: 2.793 ± 0.602
1.835IleVal: 1.835 ± 0.478
0.798IleTrp: 0.798 ± 0.216
0.638IleTyr: 0.638 ± 0.171
0.0IleXaa: 0.0 ± 0.0
Lys
6.064LysAla: 6.064 ± 0.694
0.08LysCys: 0.08 ± 0.084
4.708LysAsp: 4.708 ± 0.659
2.793LysGlu: 2.793 ± 0.439
2.314LysPhe: 2.314 ± 0.408
3.671LysGly: 3.671 ± 0.503
1.995LysHis: 1.995 ± 0.375
1.915LysIle: 1.915 ± 0.329
1.596LysLys: 1.596 ± 0.379
5.187LysLeu: 5.187 ± 0.505
0.958LysMet: 0.958 ± 0.337
2.314LysAsn: 2.314 ± 0.447
2.633LysPro: 2.633 ± 0.401
2.394LysGln: 2.394 ± 0.371
2.952LysArg: 2.952 ± 0.477
1.756LysSer: 1.756 ± 0.425
2.873LysThr: 2.873 ± 0.464
3.671LysVal: 3.671 ± 0.574
1.117LysTrp: 1.117 ± 0.321
1.117LysTyr: 1.117 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
9.815LeuAla: 9.815 ± 0.746
0.479LeuCys: 0.479 ± 0.191
5.107LeuAsp: 5.107 ± 0.638
5.027LeuGlu: 5.027 ± 0.555
2.873LeuPhe: 2.873 ± 0.371
5.905LeuGly: 5.905 ± 0.707
1.596LeuHis: 1.596 ± 0.407
5.027LeuIle: 5.027 ± 0.764
3.511LeuLys: 3.511 ± 0.633
5.905LeuLeu: 5.905 ± 0.668
2.234LeuMet: 2.234 ± 0.561
2.633LeuAsn: 2.633 ± 0.456
4.389LeuPro: 4.389 ± 0.649
3.431LeuGln: 3.431 ± 0.58
5.506LeuArg: 5.506 ± 0.628
5.346LeuSer: 5.346 ± 0.526
4.548LeuThr: 4.548 ± 0.619
5.745LeuVal: 5.745 ± 0.596
0.798LeuTrp: 0.798 ± 0.245
2.314LeuTyr: 2.314 ± 0.395
0.0LeuXaa: 0.0 ± 0.0
Met
3.591MetAla: 3.591 ± 0.667
0.16MetCys: 0.16 ± 0.099
1.915MetAsp: 1.915 ± 0.402
1.117MetGlu: 1.117 ± 0.33
1.117MetPhe: 1.117 ± 0.281
2.075MetGly: 2.075 ± 0.459
0.798MetHis: 0.798 ± 0.261
1.436MetIle: 1.436 ± 0.317
1.117MetLys: 1.117 ± 0.247
2.633MetLeu: 2.633 ± 0.445
0.479MetMet: 0.479 ± 0.198
1.117MetAsn: 1.117 ± 0.276
1.357MetPro: 1.357 ± 0.384
2.154MetGln: 2.154 ± 0.42
1.357MetArg: 1.357 ± 0.329
2.314MetSer: 2.314 ± 0.411
1.915MetThr: 1.915 ± 0.313
1.756MetVal: 1.756 ± 0.357
0.08MetTrp: 0.08 ± 0.084
0.638MetTyr: 0.638 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
4.229AsnAla: 4.229 ± 0.547
0.16AsnCys: 0.16 ± 0.18
2.474AsnAsp: 2.474 ± 0.36
2.314AsnGlu: 2.314 ± 0.437
1.117AsnPhe: 1.117 ± 0.198
2.873AsnGly: 2.873 ± 0.641
0.239AsnHis: 0.239 ± 0.11
1.676AsnIle: 1.676 ± 0.326
2.394AsnLys: 2.394 ± 0.469
3.272AsnLeu: 3.272 ± 0.469
0.958AsnMet: 0.958 ± 0.297
1.596AsnAsn: 1.596 ± 0.418
2.633AsnPro: 2.633 ± 0.37
1.915AsnGln: 1.915 ± 0.428
2.154AsnArg: 2.154 ± 0.395
2.553AsnSer: 2.553 ± 0.666
1.995AsnThr: 1.995 ± 0.336
3.351AsnVal: 3.351 ± 0.599
0.878AsnTrp: 0.878 ± 0.268
1.037AsnTyr: 1.037 ± 0.287
0.0AsnXaa: 0.0 ± 0.0
Pro
4.389ProAla: 4.389 ± 0.748
0.479ProCys: 0.479 ± 0.259
3.591ProAsp: 3.591 ± 0.417
3.75ProGlu: 3.75 ± 0.566
1.516ProPhe: 1.516 ± 0.428
3.75ProGly: 3.75 ± 0.548
0.958ProHis: 0.958 ± 0.241
1.835ProIle: 1.835 ± 0.293
2.234ProLys: 2.234 ± 0.465
3.431ProLeu: 3.431 ± 0.699
1.197ProMet: 1.197 ± 0.331
1.756ProAsn: 1.756 ± 0.491
1.357ProPro: 1.357 ± 0.41
2.314ProGln: 2.314 ± 0.388
1.436ProArg: 1.436 ± 0.317
2.394ProSer: 2.394 ± 0.532
3.192ProThr: 3.192 ± 0.549
2.633ProVal: 2.633 ± 0.443
0.798ProTrp: 0.798 ± 0.191
1.676ProTyr: 1.676 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
6.384GlnAla: 6.384 ± 0.856
0.399GlnCys: 0.399 ± 0.147
1.915GlnAsp: 1.915 ± 0.364
2.075GlnGlu: 2.075 ± 0.439
1.756GlnPhe: 1.756 ± 0.316
3.272GlnGly: 3.272 ± 0.522
1.197GlnHis: 1.197 ± 0.193
2.234GlnIle: 2.234 ± 0.377
2.075GlnLys: 2.075 ± 0.41
4.149GlnLeu: 4.149 ± 0.668
1.596GlnMet: 1.596 ± 0.339
1.995GlnAsn: 1.995 ± 0.408
1.835GlnPro: 1.835 ± 0.285
4.788GlnGln: 4.788 ± 1.453
2.633GlnArg: 2.633 ± 0.517
2.474GlnSer: 2.474 ± 0.44
3.032GlnThr: 3.032 ± 0.5
3.032GlnVal: 3.032 ± 0.538
0.798GlnTrp: 0.798 ± 0.208
1.436GlnTyr: 1.436 ± 0.331
0.0GlnXaa: 0.0 ± 0.0
Arg
5.187ArgAla: 5.187 ± 0.773
0.399ArgCys: 0.399 ± 0.187
3.671ArgAsp: 3.671 ± 0.513
4.07ArgGlu: 4.07 ± 0.622
1.995ArgPhe: 1.995 ± 0.444
3.99ArgGly: 3.99 ± 0.641
0.798ArgHis: 0.798 ± 0.313
2.394ArgIle: 2.394 ± 0.522
2.713ArgLys: 2.713 ± 0.499
5.107ArgLeu: 5.107 ± 0.666
1.756ArgMet: 1.756 ± 0.279
2.234ArgAsn: 2.234 ± 0.375
1.915ArgPro: 1.915 ± 0.402
3.272ArgGln: 3.272 ± 0.545
3.351ArgArg: 3.351 ± 0.584
3.83ArgSer: 3.83 ± 0.529
3.431ArgThr: 3.431 ± 0.578
3.83ArgVal: 3.83 ± 0.682
1.037ArgTrp: 1.037 ± 0.197
2.474ArgTyr: 2.474 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
5.985SerAla: 5.985 ± 0.55
0.559SerCys: 0.559 ± 0.321
3.431SerAsp: 3.431 ± 0.434
3.192SerGlu: 3.192 ± 0.446
2.154SerPhe: 2.154 ± 0.383
4.149SerGly: 4.149 ± 0.673
0.718SerHis: 0.718 ± 0.237
2.474SerIle: 2.474 ± 0.493
3.75SerLys: 3.75 ± 0.576
5.187SerLeu: 5.187 ± 0.482
1.835SerMet: 1.835 ± 0.337
1.915SerAsn: 1.915 ± 0.329
2.234SerPro: 2.234 ± 0.444
2.075SerGln: 2.075 ± 0.405
3.511SerArg: 3.511 ± 0.564
2.553SerSer: 2.553 ± 0.56
3.511SerThr: 3.511 ± 0.427
3.75SerVal: 3.75 ± 0.547
0.878SerTrp: 0.878 ± 0.312
1.436SerTyr: 1.436 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
7.74ThrAla: 7.74 ± 1.667
0.399ThrCys: 0.399 ± 0.161
3.112ThrAsp: 3.112 ± 0.38
2.553ThrGlu: 2.553 ± 0.514
2.075ThrPhe: 2.075 ± 0.312
5.346ThrGly: 5.346 ± 0.62
0.878ThrHis: 0.878 ± 0.311
2.474ThrIle: 2.474 ± 0.462
3.032ThrLys: 3.032 ± 0.546
4.788ThrLeu: 4.788 ± 0.816
1.835ThrMet: 1.835 ± 0.411
2.474ThrAsn: 2.474 ± 0.396
3.511ThrPro: 3.511 ± 0.487
1.357ThrGln: 1.357 ± 0.322
3.112ThrArg: 3.112 ± 0.456
2.553ThrSer: 2.553 ± 0.501
3.112ThrThr: 3.112 ± 0.723
3.83ThrVal: 3.83 ± 0.479
0.319ThrTrp: 0.319 ± 0.163
1.915ThrTyr: 1.915 ± 0.478
0.0ThrXaa: 0.0 ± 0.0
Val
7.74ValAla: 7.74 ± 0.786
0.399ValCys: 0.399 ± 0.171
3.83ValAsp: 3.83 ± 0.703
3.431ValGlu: 3.431 ± 0.5
3.272ValPhe: 3.272 ± 0.571
4.708ValGly: 4.708 ± 0.524
1.516ValHis: 1.516 ± 0.317
3.192ValIle: 3.192 ± 0.464
3.272ValLys: 3.272 ± 0.49
3.91ValLeu: 3.91 ± 0.511
1.596ValMet: 1.596 ± 0.314
2.633ValAsn: 2.633 ± 0.623
3.351ValPro: 3.351 ± 0.586
3.192ValGln: 3.192 ± 0.657
4.389ValArg: 4.389 ± 0.555
4.149ValSer: 4.149 ± 0.568
4.868ValThr: 4.868 ± 0.703
4.947ValVal: 4.947 ± 0.697
1.037ValTrp: 1.037 ± 0.221
2.474ValTyr: 2.474 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
1.197TrpAla: 1.197 ± 0.277
0.319TrpCys: 0.319 ± 0.149
0.718TrpAsp: 0.718 ± 0.208
0.798TrpGlu: 0.798 ± 0.34
0.718TrpPhe: 0.718 ± 0.239
0.958TrpGly: 0.958 ± 0.31
0.479TrpHis: 0.479 ± 0.2
0.319TrpIle: 0.319 ± 0.162
0.319TrpLys: 0.319 ± 0.153
2.234TrpLeu: 2.234 ± 0.542
0.399TrpMet: 0.399 ± 0.153
0.08TrpAsn: 0.08 ± 0.076
0.319TrpPro: 0.319 ± 0.167
0.878TrpGln: 0.878 ± 0.223
1.277TrpArg: 1.277 ± 0.307
0.638TrpSer: 0.638 ± 0.21
0.878TrpThr: 0.878 ± 0.18
1.676TrpVal: 1.676 ± 0.358
0.239TrpTrp: 0.239 ± 0.145
0.319TrpTyr: 0.319 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.91TyrAla: 3.91 ± 0.515
0.399TyrCys: 0.399 ± 0.198
1.436TyrAsp: 1.436 ± 0.358
1.436TyrGlu: 1.436 ± 0.268
1.277TyrPhe: 1.277 ± 0.241
2.793TyrGly: 2.793 ± 0.43
0.798TyrHis: 0.798 ± 0.271
1.197TyrIle: 1.197 ± 0.333
1.197TyrLys: 1.197 ± 0.27
2.234TyrLeu: 2.234 ± 0.459
0.559TyrMet: 0.559 ± 0.184
1.037TyrAsn: 1.037 ± 0.283
1.197TyrPro: 1.197 ± 0.315
1.277TyrGln: 1.277 ± 0.301
1.756TyrArg: 1.756 ± 0.312
1.436TyrSer: 1.436 ± 0.321
1.915TyrThr: 1.915 ± 0.405
1.436TyrVal: 1.436 ± 0.355
0.399TyrTrp: 0.399 ± 0.131
0.638TyrTyr: 0.638 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (12533 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski