Salimicrobium jeotgali
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2724 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K2G8T7|K2G8T7_9BACI Serine-type D-Ala-D-Ala carboxypeptidase OS=Salimicrobium jeotgali OX=1230341 GN=MJ3_07103 PE=3 SV=1
MM1 pKa = 7.62 LSNGRR6 pKa = 11.84 YY7 pKa = 8.59 FWDD10 pKa = 3.62 EE11 pKa = 3.74 AKK13 pKa = 11.02 VPGVEE18 pKa = 5.28 SDD20 pKa = 3.66 TLDD23 pKa = 3.78 EE24 pKa = 4.31 GHH26 pKa = 7.0 VIADD30 pKa = 3.61 SLGGVSNAYY39 pKa = 10.43 NITPQEE45 pKa = 4.08 STLNRR50 pKa = 11.84 HH51 pKa = 5.59 GDD53 pKa = 3.35 QAYY56 pKa = 10.88 LEE58 pKa = 4.27 DD59 pKa = 5.48 AIRR62 pKa = 11.84 NAGGATDD69 pKa = 4.44 FEE71 pKa = 5.32 AIITYY76 pKa = 9.91 PDD78 pKa = 3.66 TEE80 pKa = 4.21 TQIPSKK86 pKa = 10.49 YY87 pKa = 9.55 QYY89 pKa = 10.7 TYY91 pKa = 9.56 TIQGNEE97 pKa = 4.05 VVDD100 pKa = 3.68 TFDD103 pKa = 4.21 NVNPDD108 pKa = 4.03 DD109 pKa = 4.36 VNEE112 pKa = 4.21 SLGLTEE118 pKa = 6.26 DD119 pKa = 4.53 DD120 pKa = 5.14 DD121 pKa = 5.27 SSEE124 pKa = 4.21 SSPSFEE130 pKa = 4.04 PTTTAEE136 pKa = 4.2 EE137 pKa = 4.65 GDD139 pKa = 4.19 VSSVDD144 pKa = 3.52 ANGNGQVTIQEE155 pKa = 4.2 AKK157 pKa = 10.41 DD158 pKa = 3.36 AGFSMPITSDD168 pKa = 2.58 HH169 pKa = 6.29 WLYY172 pKa = 11.17 EE173 pKa = 3.93 YY174 pKa = 10.43 MQDD177 pKa = 3.56 RR178 pKa = 11.84 DD179 pKa = 3.3 GDD181 pKa = 4.16 GTVGEE186 pKa = 4.44
Molecular weight: 20.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.605
IPC_protein 3.617
Toseland 3.401
ProMoST 3.757
Dawson 3.605
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.439
Grimsley 3.3
Solomon 3.592
Lehninger 3.554
Nozaki 3.719
DTASelect 3.948
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.732
Patrickios 1.024
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.696
Protein with the highest isoelectric point:
>tr|K2GM93|K2GM93_9BACI Uncharacterized protein OS=Salimicrobium jeotgali OX=1230341 GN=MJ3_07108 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.12 KK14 pKa = 8.46 VHH16 pKa = 5.5 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MSTKK25 pKa = 10.06 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.37 GRR39 pKa = 11.84 KK40 pKa = 6.43 TLSAA44 pKa = 4.15
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2724
0
2724
787348
31
1596
289.0
32.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.18 ± 0.049
0.571 ± 0.014
5.357 ± 0.042
8.709 ± 0.073
4.435 ± 0.046
7.328 ± 0.049
2.09 ± 0.021
6.779 ± 0.05
6.269 ± 0.049
9.316 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.942 ± 0.024
3.912 ± 0.031
3.69 ± 0.027
3.348 ± 0.029
4.704 ± 0.04
6.075 ± 0.042
5.538 ± 0.028
7.278 ± 0.039
1.015 ± 0.019
3.463 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here