Amino acid dipepetide frequency for Lottia gigantea (Giant owl limpet)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.467AlaAla: 3.467 ± 0.029	1.072AlaCys: 1.072 ± 0.015	2.748AlaAsp: 2.748 ± 0.022	3.055AlaGlu: 3.055 ± 0.023	2.116AlaPhe: 2.116 ± 0.015	2.975AlaGly: 2.975 ± 0.024	1.006AlaHis: 1.006 ± 0.012	3.246AlaIle: 3.246 ± 0.025	3.29AlaLys: 3.29 ± 0.025	4.474AlaLeu: 4.474 ± 0.032	1.336AlaMet: 1.336 ± 0.016	2.369AlaAsn: 2.369 ± 0.016	1.976AlaPro: 1.976 ± 0.018	1.765AlaGln: 1.765 ± 0.018	2.343AlaArg: 2.343 ± 0.022	3.888AlaSer: 3.888 ± 0.026	2.989AlaThr: 2.989 ± 0.026	3.608AlaVal: 3.608 ± 0.022	0.547AlaTrp: 0.547 ± 0.008	1.545AlaTyr: 1.545 ± 0.015	0.0AlaXaa: 0.0 ± 0.0
Cys	0.911CysAla: 0.911 ± 0.01	0.612CysCys: 0.612 ± 0.013	1.454CysAsp: 1.454 ± 0.023	1.287CysGlu: 1.287 ± 0.018	1.024CysPhe: 1.024 ± 0.017	1.486CysGly: 1.486 ± 0.019	0.624CysHis: 0.624 ± 0.012	1.493CysIle: 1.493 ± 0.019	1.491CysLys: 1.491 ± 0.019	2.171CysLeu: 2.171 ± 0.022	0.529CysMet: 0.529 ± 0.01	1.204CysAsn: 1.204 ± 0.018	1.121CysPro: 1.121 ± 0.016	1.041CysGln: 1.041 ± 0.019	1.105CysArg: 1.105 ± 0.014	1.884CysSer: 1.884 ± 0.023	1.281CysThr: 1.281 ± 0.019	1.365CysVal: 1.365 ± 0.017	0.228CysTrp: 0.228 ± 0.005	0.859CysTyr: 0.859 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	2.603AspAla: 2.603 ± 0.022	1.358AspCys: 1.358 ± 0.019	4.158AspAsp: 4.158 ± 0.039	3.998AspGlu: 3.998 ± 0.03	2.504AspPhe: 2.504 ± 0.017	3.521AspGly: 3.521 ± 0.03	1.257AspHis: 1.257 ± 0.02	4.351AspIle: 4.351 ± 0.03	4.042AspLys: 4.042 ± 0.033	4.999AspLeu: 4.999 ± 0.026	1.323AspMet: 1.323 ± 0.013	3.264AspAsn: 3.264 ± 0.03	2.317AspPro: 2.317 ± 0.024	1.967AspGln: 1.967 ± 0.02	2.48AspArg: 2.48 ± 0.024	4.516AspSer: 4.516 ± 0.033	3.122AspThr: 3.122 ± 0.025	3.922AspVal: 3.922 ± 0.027	0.686AspTrp: 0.686 ± 0.013	2.177AspTyr: 2.177 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	3.191GluAla: 3.191 ± 0.027	1.234GluCys: 1.234 ± 0.019	4.042GluAsp: 4.042 ± 0.028	5.492GluGlu: 5.492 ± 0.057	2.473GluPhe: 2.473 ± 0.017	2.942GluGly: 2.942 ± 0.021	1.238GluHis: 1.238 ± 0.016	4.198GluIle: 4.198 ± 0.03	5.141GluLys: 5.141 ± 0.041	5.258GluLeu: 5.258 ± 0.033	1.56GluMet: 1.56 ± 0.015	3.76GluAsn: 3.76 ± 0.026	2.083GluPro: 2.083 ± 0.022	2.196GluGln: 2.196 ± 0.022	3.068GluArg: 3.068 ± 0.025	4.315GluSer: 4.315 ± 0.031	3.655GluThr: 3.655 ± 0.032	3.626GluVal: 3.626 ± 0.031	0.669GluTrp: 0.669 ± 0.011	2.196GluTyr: 2.196 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	1.848PheAla: 1.848 ± 0.015	1.033PheCys: 1.033 ± 0.015	2.551PheAsp: 2.551 ± 0.021	2.258PheGlu: 2.258 ± 0.019	1.734PhePhe: 1.734 ± 0.018	2.506PheGly: 2.506 ± 0.021	1.089PheHis: 1.089 ± 0.016	2.938PheIle: 2.938 ± 0.028	2.73PheLys: 2.73 ± 0.02	3.875PheLeu: 3.875 ± 0.032	0.957PheMet: 0.957 ± 0.012	2.336PheAsn: 2.336 ± 0.022	1.699PhePro: 1.699 ± 0.016	1.833PheGln: 1.833 ± 0.019	1.949PheArg: 1.949 ± 0.019	3.266PheSer: 3.266 ± 0.022	2.718PheThr: 2.718 ± 0.021	2.574PheVal: 2.574 ± 0.02	0.508PheTrp: 0.508 ± 0.009	1.653PheTyr: 1.653 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	2.674GlyAla: 2.674 ± 0.027	1.206GlyCys: 1.206 ± 0.016	3.191GlyAsp: 3.191 ± 0.025	3.322GlyGlu: 3.322 ± 0.032	2.636GlyPhe: 2.636 ± 0.024	3.789GlyGly: 3.789 ± 0.039	1.385GlyHis: 1.385 ± 0.018	3.527GlyIle: 3.527 ± 0.022	3.811GlyLys: 3.811 ± 0.029	4.58GlyLeu: 4.58 ± 0.031	1.371GlyMet: 1.371 ± 0.021	2.954GlyAsn: 2.954 ± 0.024	1.907GlyPro: 1.907 ± 0.027	2.179GlyGln: 2.179 ± 0.023	2.776GlyArg: 2.776 ± 0.04	4.4GlySer: 4.4 ± 0.032	3.12GlyThr: 3.12 ± 0.026	3.457GlyVal: 3.457 ± 0.031	0.686GlyTrp: 0.686 ± 0.009	2.184GlyTyr: 2.184 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	0.991HisAla: 0.991 ± 0.012	0.642HisCys: 0.642 ± 0.013	1.219HisAsp: 1.219 ± 0.015	1.171HisGlu: 1.171 ± 0.015	1.07HisPhe: 1.07 ± 0.015	1.362HisGly: 1.362 ± 0.015	0.907HisHis: 0.907 ± 0.022	1.586HisIle: 1.586 ± 0.016	1.461HisLys: 1.461 ± 0.02	2.343HisLeu: 2.343 ± 0.022	0.581HisMet: 0.581 ± 0.013	1.237HisAsn: 1.237 ± 0.016	1.21HisPro: 1.21 ± 0.015	1.059HisGln: 1.059 ± 0.014	1.22HisArg: 1.22 ± 0.016	1.928HisSer: 1.928 ± 0.023	1.397HisThr: 1.397 ± 0.021	1.497HisVal: 1.497 ± 0.02	0.303HisTrp: 0.303 ± 0.008	0.99HisTyr: 0.99 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	3.136IleAla: 3.136 ± 0.023	1.553IleCys: 1.553 ± 0.018	3.851IleAsp: 3.851 ± 0.031	3.856IleGlu: 3.856 ± 0.027	2.879IlePhe: 2.879 ± 0.029	3.26IleGly: 3.26 ± 0.025	1.624IleHis: 1.624 ± 0.016	4.222IleIle: 4.222 ± 0.033	4.316IleLys: 4.316 ± 0.03	6.062IleLeu: 6.062 ± 0.039	1.343IleMet: 1.343 ± 0.017	3.501IleAsn: 3.501 ± 0.03	3.186IlePro: 3.186 ± 0.026	2.818IleGln: 2.818 ± 0.025	2.928IleArg: 2.928 ± 0.023	5.123IleSer: 5.123 ± 0.033	3.92IleThr: 3.92 ± 0.032	3.995IleVal: 3.995 ± 0.029	0.653IleTrp: 0.653 ± 0.011	2.312IleTyr: 2.312 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.464LysAla: 3.464 ± 0.027	1.553LysCys: 1.553 ± 0.017	4.165LysAsp: 4.165 ± 0.03	4.863LysGlu: 4.863 ± 0.036	2.514LysPhe: 2.514 ± 0.019	3.248LysGly: 3.248 ± 0.041	1.725LysHis: 1.725 ± 0.02	4.36LysIle: 4.36 ± 0.028	5.731LysLys: 5.731 ± 0.05	6.168LysLeu: 6.168 ± 0.042	1.61LysMet: 1.61 ± 0.013	3.526LysAsn: 3.526 ± 0.025	3.331LysPro: 3.331 ± 0.034	2.853LysGln: 2.853 ± 0.025	3.815LysArg: 3.815 ± 0.029	5.32LysSer: 5.32 ± 0.039	4.325LysThr: 4.325 ± 0.029	3.968LysVal: 3.968 ± 0.026	0.758LysTrp: 0.758 ± 0.01	2.633LysTyr: 2.633 ± 0.019	0.001LysXaa: 0.001 ± 0.0
Leu	4.722LeuAla: 4.722 ± 0.032	1.968LeuCys: 1.968 ± 0.019	4.999LeuAsp: 4.999 ± 0.03	5.49LeuGlu: 5.49 ± 0.033	3.706LeuPhe: 3.706 ± 0.03	4.315LeuGly: 4.315 ± 0.029	2.243LeuHis: 2.243 ± 0.025	5.113LeuIle: 5.113 ± 0.035	6.437LeuLys: 6.437 ± 0.038	8.083LeuLeu: 8.083 ± 0.053	2.048LeuMet: 2.048 ± 0.017	4.645LeuAsn: 4.645 ± 0.029	4.455LeuPro: 4.455 ± 0.029	4.126LeuGln: 4.126 ± 0.032	4.161LeuArg: 4.161 ± 0.034	7.119LeuSer: 7.119 ± 0.043	5.35LeuThr: 5.35 ± 0.036	5.125LeuVal: 5.125 ± 0.035	0.886LeuTrp: 0.886 ± 0.012	3.31LeuTyr: 3.31 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	1.637MetAla: 1.637 ± 0.016	0.529MetCys: 0.529 ± 0.01	1.302MetAsp: 1.302 ± 0.013	1.542MetGlu: 1.542 ± 0.014	1.047MetPhe: 1.047 ± 0.013	1.199MetGly: 1.199 ± 0.017	0.36MetHis: 0.36 ± 0.006	1.385MetIle: 1.385 ± 0.019	1.834MetLys: 1.834 ± 0.017	1.979MetLeu: 1.979 ± 0.02	0.697MetMet: 0.697 ± 0.014	1.241MetAsn: 1.241 ± 0.012	0.904MetPro: 0.904 ± 0.012	0.694MetGln: 0.694 ± 0.012	0.975MetArg: 0.975 ± 0.016	1.901MetSer: 1.901 ± 0.017	1.387MetThr: 1.387 ± 0.018	1.526MetVal: 1.526 ± 0.015	0.249MetTrp: 0.249 ± 0.007	0.768MetTyr: 0.768 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.259AsnAla: 2.259 ± 0.018	1.322AsnCys: 1.322 ± 0.022	3.086AsnAsp: 3.086 ± 0.026	3.022AsnGlu: 3.022 ± 0.023	2.235AsnPhe: 2.235 ± 0.021	3.341AsnGly: 3.341 ± 0.026	1.346AsnHis: 1.346 ± 0.019	4.156AsnIle: 4.156 ± 0.032	3.689AsnLys: 3.689 ± 0.025	4.728AsnLeu: 4.728 ± 0.03	1.267AsnMet: 1.267 ± 0.014	3.46AsnAsn: 3.46 ± 0.036	2.505AsnPro: 2.505 ± 0.022	2.29AsnGln: 2.29 ± 0.024	2.464AsnArg: 2.464 ± 0.024	4.341AsnSer: 4.341 ± 0.034	3.102AsnThr: 3.102 ± 0.028	3.353AsnVal: 3.353 ± 0.026	0.626AsnTrp: 0.626 ± 0.01	1.95AsnTyr: 1.95 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	2.297ProAla: 2.297 ± 0.024	0.836ProCys: 0.836 ± 0.016	2.628ProAsp: 2.628 ± 0.021	2.973ProGlu: 2.973 ± 0.026	1.751ProPhe: 1.751 ± 0.018	2.629ProGly: 2.629 ± 0.032	0.999ProHis: 0.999 ± 0.014	2.601ProIle: 2.601 ± 0.024	2.973ProLys: 2.973 ± 0.029	3.482ProLeu: 3.482 ± 0.023	0.902ProMet: 0.902 ± 0.014	2.408ProAsn: 2.408 ± 0.021	2.98ProPro: 2.98 ± 0.035	1.773ProGln: 1.773 ± 0.019	2.059ProArg: 2.059 ± 0.022	3.908ProSer: 3.908 ± 0.032	3.039ProThr: 3.039 ± 0.033	3.133ProVal: 3.133 ± 0.028	0.464ProTrp: 0.464 ± 0.007	1.625ProTyr: 1.625 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	2.105GlnAla: 2.105 ± 0.019	1.012GlnCys: 1.012 ± 0.018	2.048GlnAsp: 2.048 ± 0.02	2.52GlnGlu: 2.52 ± 0.023	1.573GlnPhe: 1.573 ± 0.019	1.92GlnGly: 1.92 ± 0.017	1.042GlnHis: 1.042 ± 0.016	2.562GlnIle: 2.562 ± 0.021	2.762GlnLys: 2.762 ± 0.022	3.667GlnLeu: 3.667 ± 0.032	0.967GlnMet: 0.967 ± 0.011	2.318GlnAsn: 2.318 ± 0.02	1.937GlnPro: 1.937 ± 0.024	2.33GlnGln: 2.33 ± 0.042	1.939GlnArg: 1.939 ± 0.018	3.124GlnSer: 3.124 ± 0.025	2.528GlnThr: 2.528 ± 0.025	2.312GlnVal: 2.312 ± 0.022	0.474GlnTrp: 0.474 ± 0.01	1.55GlnTyr: 1.55 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	2.251ArgAla: 2.251 ± 0.019	1.058ArgCys: 1.058 ± 0.016	2.511ArgAsp: 2.511 ± 0.022	2.873ArgGlu: 2.873 ± 0.027	1.94ArgPhe: 1.94 ± 0.017	2.402ArgGly: 2.402 ± 0.029	1.395ArgHis: 1.395 ± 0.015	2.844ArgIle: 2.844 ± 0.027	3.799ArgLys: 3.799 ± 0.041	4.293ArgLeu: 4.293 ± 0.028	1.099ArgMet: 1.099 ± 0.012	2.699ArgAsn: 2.699 ± 0.023	2.127ArgPro: 2.127 ± 0.021	2.114ArgGln: 2.114 ± 0.019	3.146ArgArg: 3.146 ± 0.035	3.553ArgSer: 3.553 ± 0.035	2.497ArgThr: 2.497 ± 0.022	2.633ArgVal: 2.633 ± 0.021	0.526ArgTrp: 0.526 ± 0.009	1.849ArgTyr: 1.849 ± 0.022	0.0ArgXaa: 0.0 ± 0.0
Ser	3.787SerAla: 3.787 ± 0.024	1.732SerCys: 1.732 ± 0.021	4.777SerAsp: 4.777 ± 0.032	4.621SerGlu: 4.621 ± 0.035	3.261SerPhe: 3.261 ± 0.025	4.611SerGly: 4.611 ± 0.031	1.912SerHis: 1.912 ± 0.02	4.708SerIle: 4.708 ± 0.033	5.186SerLys: 5.186 ± 0.037	7.072SerLeu: 7.072 ± 0.043	1.756SerMet: 1.756 ± 0.02	4.357SerAsn: 4.357 ± 0.035	3.945SerPro: 3.945 ± 0.043	3.243SerGln: 3.243 ± 0.028	3.74SerArg: 3.74 ± 0.028	7.883SerSer: 7.883 ± 0.07	5.07SerThr: 5.07 ± 0.041	5.089SerVal: 5.089 ± 0.034	0.807SerTrp: 0.807 ± 0.011	2.638SerTyr: 2.638 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	3.264ThrAla: 3.264 ± 0.024	1.492ThrCys: 1.492 ± 0.023	3.551ThrAsp: 3.551 ± 0.027	3.697ThrGlu: 3.697 ± 0.038	2.498ThrPhe: 2.498 ± 0.024	3.673ThrGly: 3.673 ± 0.033	1.272ThrHis: 1.272 ± 0.017	3.828ThrIle: 3.828 ± 0.029	3.693ThrLys: 3.693 ± 0.027	5.049ThrLeu: 5.049 ± 0.03	1.299ThrMet: 1.299 ± 0.017	3.12ThrAsn: 3.12 ± 0.026	3.148ThrPro: 3.148 ± 0.031	2.108ThrGln: 2.108 ± 0.019	2.455ThrArg: 2.455 ± 0.025	5.259ThrSer: 5.259 ± 0.048	4.517ThrThr: 4.517 ± 0.068	4.23ThrVal: 4.23 ± 0.034	0.679ThrTrp: 0.679 ± 0.012	1.911ThrTyr: 1.911 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	3.219ValAla: 3.219 ± 0.027	1.686ValCys: 1.686 ± 0.022	3.667ValAsp: 3.667 ± 0.027	3.784ValGlu: 3.784 ± 0.029	2.821ValPhe: 2.821 ± 0.025	3.248ValGly: 3.248 ± 0.029	1.462ValHis: 1.462 ± 0.016	4.125ValIle: 4.125 ± 0.034	4.306ValLys: 4.306 ± 0.03	5.596ValLeu: 5.596 ± 0.034	1.413ValMet: 1.413 ± 0.016	3.36ValAsn: 3.36 ± 0.025	2.697ValPro: 2.697 ± 0.021	2.307ValGln: 2.307 ± 0.019	2.626ValArg: 2.626 ± 0.02	4.791ValSer: 4.791 ± 0.032	3.882ValThr: 3.882 ± 0.031	4.039ValVal: 4.039 ± 0.035	0.718ValTrp: 0.718 ± 0.01	2.255ValTyr: 2.255 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	0.529TrpAla: 0.529 ± 0.009	0.281TrpCys: 0.281 ± 0.009	0.609TrpAsp: 0.609 ± 0.01	0.594TrpGlu: 0.594 ± 0.008	0.522TrpPhe: 0.522 ± 0.008	0.6TrpGly: 0.6 ± 0.01	0.239TrpHis: 0.239 ± 0.006	0.739TrpIle: 0.739 ± 0.01	0.893TrpLys: 0.893 ± 0.01	1.025TrpLeu: 1.025 ± 0.013	0.308TrpMet: 0.308 ± 0.008	0.641TrpAsn: 0.641 ± 0.011	0.363TrpPro: 0.363 ± 0.007	0.423TrpGln: 0.423 ± 0.008	0.568TrpArg: 0.568 ± 0.009	0.852TrpSer: 0.852 ± 0.01	0.671TrpThr: 0.671 ± 0.011	0.558TrpVal: 0.558 ± 0.009	0.174TrpTrp: 0.174 ± 0.006	0.479TrpTyr: 0.479 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.458TyrAla: 1.458 ± 0.013	0.994TyrCys: 0.994 ± 0.016	2.009TyrAsp: 2.009 ± 0.018	1.917TyrGlu: 1.917 ± 0.019	1.752TyrPhe: 1.752 ± 0.021	2.128TyrGly: 2.128 ± 0.02	1.054TyrHis: 1.054 ± 0.018	2.561TyrIle: 2.561 ± 0.026	2.344TyrLys: 2.344 ± 0.025	3.397TyrLeu: 3.397 ± 0.03	0.786TyrMet: 0.786 ± 0.012	2.144TyrAsn: 2.144 ± 0.024	1.57TyrPro: 1.57 ± 0.019	1.57TyrGln: 1.57 ± 0.019	1.789TyrArg: 1.789 ± 0.018	2.823TyrSer: 2.823 ± 0.024	2.171TyrThr: 2.171 ± 0.025	1.992TyrVal: 1.992 ± 0.02	0.424TyrTrp: 0.424 ± 0.009	1.608TyrTyr: 1.608 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 23675 proteins (8983955 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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