Paenibacillus xerothermodurans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3922 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W1NSW4|A0A2W1NSW4_9BACL Thiamine biosynthesis protein ThiS OS=Paenibacillus xerothermodurans OX=1977292 GN=thiS PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 4.06HH4 pKa = 6.76IHH6 pKa = 7.61DD7 pKa = 4.29EE8 pKa = 4.56SCDD11 pKa = 3.67HH12 pKa = 7.23DD13 pKa = 5.4HH14 pKa = 7.75DD15 pKa = 6.18DD16 pKa = 5.24DD17 pKa = 4.85VFIVTDD23 pKa = 3.53KK24 pKa = 11.54DD25 pKa = 3.96GVEE28 pKa = 4.35HH29 pKa = 6.39EE30 pKa = 4.38MVMVYY35 pKa = 9.02TFEE38 pKa = 6.65ASDD41 pKa = 3.34QPYY44 pKa = 10.88AVLLDD49 pKa = 4.18RR50 pKa = 11.84NDD52 pKa = 3.77PEE54 pKa = 5.88ADD56 pKa = 3.2GVIFRR61 pKa = 11.84IEE63 pKa = 4.05EE64 pKa = 4.1EE65 pKa = 4.04NEE67 pKa = 3.55EE68 pKa = 4.32AYY70 pKa = 10.59LASIEE75 pKa = 5.58DD76 pKa = 3.6EE77 pKa = 4.54EE78 pKa = 4.13EE79 pKa = 3.88WEE81 pKa = 4.26RR82 pKa = 11.84VVQIYY87 pKa = 9.58NQIIEE92 pKa = 4.29EE93 pKa = 4.23QEE95 pKa = 4.22TQQ97 pKa = 3.01

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W1N3H1|A0A2W1N3H1_9BACL Uncharacterized protein OS=Paenibacillus xerothermodurans OX=1977292 GN=CBW46_019485 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.92PNVRR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 8.89KK14 pKa = 8.64VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3922

0

3922

1195824

18

3618

304.9

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.016 ± 0.051

0.841 ± 0.013

5.148 ± 0.03

6.357 ± 0.037

3.992 ± 0.028

7.507 ± 0.036

2.37 ± 0.022

6.497 ± 0.036

5.038 ± 0.038

9.899 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.018

3.621 ± 0.028

4.216 ± 0.026

4.161 ± 0.024

5.243 ± 0.036

5.851 ± 0.026

5.427 ± 0.028

7.444 ± 0.033

1.22 ± 0.015

3.286 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski