Bacillus sp. KQ-3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M7TN76|A0A3M7TN76_9BACI Aminoglycoside N(3)-acetyltransferase OS=Bacillus sp. KQ-3 OX=2483800 GN=EBO34_18005 PE=3 SV=1
MM1 pKa = 7.49TGLLFSTTAPAAQADD16 pKa = 4.02AEE18 pKa = 4.31DD19 pKa = 4.26TEE21 pKa = 4.75KK22 pKa = 11.02LVIMGTTDD30 pKa = 1.99IHH32 pKa = 8.52AYY34 pKa = 8.23MMPYY38 pKa = 10.43DD39 pKa = 4.15YY40 pKa = 11.13MNDD43 pKa = 3.85TVDD46 pKa = 3.55DD47 pKa = 4.43TYY49 pKa = 11.98GLTKK53 pKa = 10.41VHH55 pKa = 6.2TLVEE59 pKa = 4.86EE60 pKa = 4.12IRR62 pKa = 11.84SEE64 pKa = 4.35HH65 pKa = 6.71EE66 pKa = 3.77NTLLLDD72 pKa = 4.05NGDD75 pKa = 4.51IIQGSIFGDD84 pKa = 3.42MLALVDD90 pKa = 4.45PVGADD95 pKa = 3.67EE96 pKa = 4.5PHH98 pKa = 7.64VIMDD102 pKa = 4.29AMNMMGYY109 pKa = 10.23DD110 pKa = 3.4AATLGNHH117 pKa = 5.92EE118 pKa = 4.99FNFGLDD124 pKa = 3.74YY125 pKa = 11.17LDD127 pKa = 3.48EE128 pKa = 5.03TIAKK132 pKa = 10.13ADD134 pKa = 4.17FPWLSANVYY143 pKa = 10.87NDD145 pKa = 3.13GTEE148 pKa = 3.95DD149 pKa = 4.3LRR151 pKa = 11.84YY152 pKa = 10.22DD153 pKa = 3.95PYY155 pKa = 11.58AIIEE159 pKa = 4.17KK160 pKa = 9.93VINGEE165 pKa = 4.11TLNVGVIGLVPPQIMTWDD183 pKa = 3.63RR184 pKa = 11.84VHH186 pKa = 7.59LDD188 pKa = 2.98GNVYY192 pKa = 10.29VNEE195 pKa = 4.06IVEE198 pKa = 4.33SAKK201 pKa = 10.36TYY203 pKa = 9.04VPQMKK208 pKa = 10.32EE209 pKa = 3.46EE210 pKa = 4.24GADD213 pKa = 3.03IVVAVAHH220 pKa = 6.19SGINPAEE227 pKa = 4.44DD228 pKa = 3.31ASEE231 pKa = 3.89NAGYY235 pKa = 10.06QLSQVEE241 pKa = 5.3GIDD244 pKa = 4.29AMILGHH250 pKa = 5.39QHH252 pKa = 5.81NVFPGTDD259 pKa = 3.24AYY261 pKa = 11.46SGIDD265 pKa = 4.07GIDD268 pKa = 3.44VEE270 pKa = 4.82AGTVNGVPTVMPGAWGSHH288 pKa = 5.55LGVIEE293 pKa = 5.37LDD295 pKa = 3.57LTNTDD300 pKa = 3.73GQWEE304 pKa = 4.37VAGFDD309 pKa = 3.45SEE311 pKa = 4.44ARR313 pKa = 11.84EE314 pKa = 3.85TAAYY318 pKa = 7.98EE319 pKa = 3.79PHH321 pKa = 7.4PDD323 pKa = 3.13MVSLVEE329 pKa = 4.2EE330 pKa = 4.11THH332 pKa = 6.11EE333 pKa = 3.98ATIEE337 pKa = 4.19YY338 pKa = 10.19VNSPVGDD345 pKa = 3.6TATDD349 pKa = 3.88LNTFFSRR356 pKa = 11.84VMDD359 pKa = 3.83NEE361 pKa = 4.07VVQLVNDD368 pKa = 3.78AQLDD372 pKa = 3.82YY373 pKa = 10.76MEE375 pKa = 5.24QNFASTEE382 pKa = 4.06YY383 pKa = 10.57EE384 pKa = 4.06DD385 pKa = 3.82MAMLSAAAPFRR396 pKa = 11.84AGRR399 pKa = 11.84GGAYY403 pKa = 9.56TNVEE407 pKa = 4.27EE408 pKa = 4.42GSIAIRR414 pKa = 11.84DD415 pKa = 3.63VNDD418 pKa = 2.99IYY420 pKa = 11.46VYY422 pKa = 10.68PNTLHH427 pKa = 5.3VVKK430 pKa = 11.12VNGTEE435 pKa = 3.73LHH437 pKa = 5.83NWLEE441 pKa = 4.02RR442 pKa = 11.84SAEE445 pKa = 3.98NFLQIDD451 pKa = 4.08PDD453 pKa = 3.88SSDD456 pKa = 2.99RR457 pKa = 11.84QYY459 pKa = 11.84VINTDD464 pKa = 3.76FASFDD469 pKa = 3.51FDD471 pKa = 3.94TIEE474 pKa = 4.1GVEE477 pKa = 3.99YY478 pKa = 10.59QIDD481 pKa = 3.62VTQPVGEE488 pKa = 4.42RR489 pKa = 11.84IVGLTYY495 pKa = 10.38EE496 pKa = 4.67GEE498 pKa = 4.33DD499 pKa = 3.25VTEE502 pKa = 3.94EE503 pKa = 4.7DD504 pKa = 3.96EE505 pKa = 5.87FLVATNNYY513 pKa = 8.71RR514 pKa = 11.84AGGGGAHH521 pKa = 6.97IDD523 pKa = 3.64GVEE526 pKa = 4.16TVLASTDD533 pKa = 3.29EE534 pKa = 3.97NRR536 pKa = 11.84EE537 pKa = 3.9VIIDD541 pKa = 3.84YY542 pKa = 10.71IRR544 pKa = 11.84DD545 pKa = 3.38HH546 pKa = 7.38DD547 pKa = 4.4GPLEE551 pKa = 4.15VEE553 pKa = 4.55VTNNWSLVPFEE564 pKa = 4.51TDD566 pKa = 2.7AQVVFEE572 pKa = 5.09SDD574 pKa = 3.39LQGEE578 pKa = 4.5PLADD582 pKa = 3.4TTPFVSFVEE591 pKa = 4.69TVNDD595 pKa = 3.73TTGLFQIHH603 pKa = 5.86PQEE606 pKa = 4.25HH607 pKa = 6.24VEE609 pKa = 5.09DD610 pKa = 5.45GDD612 pKa = 6.18DD613 pKa = 3.6EE614 pKa = 6.82DD615 pKa = 5.51EE616 pKa = 5.42DD617 pKa = 4.47DD618 pKa = 4.9RR619 pKa = 11.84PGNRR623 pKa = 11.84PPHH626 pKa = 6.57AGQPGPPPHH635 pKa = 7.17AGQPGPPPHH644 pKa = 7.07ANN646 pKa = 3.0

Molecular weight: 70.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M7TQW7|A0A3M7TQW7_9BACI Flagellar protein FlgN OS=Bacillus sp. KQ-3 OX=2483800 GN=EBO34_12640 PE=4 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.18RR13 pKa = 11.84KK14 pKa = 8.6KK15 pKa = 9.25VHH17 pKa = 5.37GFRR20 pKa = 11.84NRR22 pKa = 11.84MSTKK26 pKa = 9.22NGRR29 pKa = 11.84RR30 pKa = 11.84VLANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.69GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3944

0

3944

1152186

25

1895

292.1

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.043

0.698 ± 0.012

5.532 ± 0.036

8.08 ± 0.056

4.527 ± 0.032

7.365 ± 0.032

2.187 ± 0.02

6.796 ± 0.041

6.151 ± 0.046

9.514 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.02

3.923 ± 0.026

3.769 ± 0.023

3.362 ± 0.024

4.589 ± 0.029

5.919 ± 0.029

5.516 ± 0.023

7.456 ± 0.03

1.088 ± 0.014

3.377 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski