Amino acid dipepetide frequency for Bacillus sp. KQ-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.419AlaAla: 6.419 ± 0.102
0.653AlaCys: 0.653 ± 0.027
3.825AlaAsp: 3.825 ± 0.062
5.156AlaGlu: 5.156 ± 0.083
3.608AlaPhe: 3.608 ± 0.065
6.231AlaGly: 6.231 ± 0.092
1.401AlaHis: 1.401 ± 0.038
5.088AlaIle: 5.088 ± 0.077
4.059AlaLys: 4.059 ± 0.069
7.431AlaLeu: 7.431 ± 0.087
2.166AlaMet: 2.166 ± 0.041
2.349AlaAsn: 2.349 ± 0.05
2.248AlaPro: 2.248 ± 0.058
2.029AlaGln: 2.029 ± 0.046
3.104AlaArg: 3.104 ± 0.057
4.215AlaSer: 4.215 ± 0.076
3.308AlaThr: 3.308 ± 0.062
6.331AlaVal: 6.331 ± 0.091
0.728AlaTrp: 0.728 ± 0.028
2.288AlaTyr: 2.288 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.423CysAla: 0.423 ± 0.022
0.116CysCys: 0.116 ± 0.01
0.407CysAsp: 0.407 ± 0.021
0.518CysGlu: 0.518 ± 0.025
0.341CysPhe: 0.341 ± 0.018
0.677CysGly: 0.677 ± 0.024
0.181CysHis: 0.181 ± 0.015
0.398CysIle: 0.398 ± 0.018
0.342CysLys: 0.342 ± 0.015
0.648CysLeu: 0.648 ± 0.021
0.176CysMet: 0.176 ± 0.012
0.25CysAsn: 0.25 ± 0.015
0.36CysPro: 0.36 ± 0.018
0.177CysGln: 0.177 ± 0.012
0.33CysArg: 0.33 ± 0.017
0.482CysSer: 0.482 ± 0.02
0.4CysThr: 0.4 ± 0.017
0.443CysVal: 0.443 ± 0.021
0.069CysTrp: 0.069 ± 0.009
0.244CysTyr: 0.244 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.671AspAla: 3.671 ± 0.07
0.341AspCys: 0.341 ± 0.017
3.194AspAsp: 3.194 ± 0.062
5.267AspGlu: 5.267 ± 0.081
2.386AspPhe: 2.386 ± 0.052
3.862AspGly: 3.862 ± 0.071
1.404AspHis: 1.404 ± 0.041
3.738AspIle: 3.738 ± 0.063
2.784AspLys: 2.784 ± 0.055
5.336AspLeu: 5.336 ± 0.077
1.511AspMet: 1.511 ± 0.034
1.891AspAsn: 1.891 ± 0.04
2.346AspPro: 2.346 ± 0.055
2.026AspGln: 2.026 ± 0.05
2.96AspArg: 2.96 ± 0.05
2.634AspSer: 2.634 ± 0.049
2.698AspThr: 2.698 ± 0.05
4.4AspVal: 4.4 ± 0.066
0.715AspTrp: 0.715 ± 0.026
2.158AspTyr: 2.158 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
6.443GluAla: 6.443 ± 0.085
0.419GluCys: 0.419 ± 0.02
4.71GluAsp: 4.71 ± 0.083
8.69GluGlu: 8.69 ± 0.115
2.413GluPhe: 2.413 ± 0.048
5.41GluGly: 5.41 ± 0.083
1.627GluHis: 1.627 ± 0.039
4.581GluIle: 4.581 ± 0.074
6.423GluLys: 6.423 ± 0.089
7.146GluLeu: 7.146 ± 0.091
2.507GluMet: 2.507 ± 0.053
3.474GluAsn: 3.474 ± 0.058
2.395GluPro: 2.395 ± 0.051
3.154GluGln: 3.154 ± 0.05
4.28GluArg: 4.28 ± 0.063
3.881GluSer: 3.881 ± 0.061
4.306GluThr: 4.306 ± 0.066
5.606GluVal: 5.606 ± 0.078
0.982GluTrp: 0.982 ± 0.031
2.348GluTyr: 2.348 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
3.14PheAla: 3.14 ± 0.064
0.348PheCys: 0.348 ± 0.02
2.531PheAsp: 2.531 ± 0.051
3.006PheGlu: 3.006 ± 0.051
2.48PhePhe: 2.48 ± 0.062
3.333PheGly: 3.333 ± 0.061
1.019PheHis: 1.019 ± 0.031
3.46PheIle: 3.46 ± 0.067
2.07PheLys: 2.07 ± 0.044
4.557PheLeu: 4.557 ± 0.087
1.183PheMet: 1.183 ± 0.032
1.753PheAsn: 1.753 ± 0.044
1.683PhePro: 1.683 ± 0.035
1.446PheGln: 1.446 ± 0.039
1.711PheArg: 1.711 ± 0.04
3.249PheSer: 3.249 ± 0.054
2.823PheThr: 2.823 ± 0.05
3.278PheVal: 3.278 ± 0.054
0.49PheTrp: 0.49 ± 0.025
1.714PheTyr: 1.714 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.44GlyAla: 5.44 ± 0.083
0.643GlyCys: 0.643 ± 0.027
3.805GlyAsp: 3.805 ± 0.064
5.486GlyGlu: 5.486 ± 0.08
3.685GlyPhe: 3.685 ± 0.054
5.52GlyGly: 5.52 ± 0.083
1.512GlyHis: 1.512 ± 0.043
5.687GlyIle: 5.687 ± 0.077
4.688GlyLys: 4.688 ± 0.062
6.883GlyLeu: 6.883 ± 0.079
2.349GlyMet: 2.349 ± 0.048
2.677GlyAsn: 2.677 ± 0.062
2.056GlyPro: 2.056 ± 0.046
2.182GlyGln: 2.182 ± 0.051
3.272GlyArg: 3.272 ± 0.061
4.318GlySer: 4.318 ± 0.062
4.261GlyThr: 4.261 ± 0.068
5.543GlyVal: 5.543 ± 0.076
0.89GlyTrp: 0.89 ± 0.026
2.753GlyTyr: 2.753 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.35HisAla: 1.35 ± 0.033
0.183HisCys: 0.183 ± 0.015
1.141HisAsp: 1.141 ± 0.033
1.618HisGlu: 1.618 ± 0.039
1.11HisPhe: 1.11 ± 0.034
1.577HisGly: 1.577 ± 0.042
0.742HisHis: 0.742 ± 0.027
1.401HisIle: 1.401 ± 0.031
0.987HisLys: 0.987 ± 0.035
2.251HisLeu: 2.251 ± 0.05
0.563HisMet: 0.563 ± 0.024
0.772HisAsn: 0.772 ± 0.026
1.174HisPro: 1.174 ± 0.039
0.817HisGln: 0.817 ± 0.024
1.042HisArg: 1.042 ± 0.033
1.208HisSer: 1.208 ± 0.038
1.215HisThr: 1.215 ± 0.034
1.603HisVal: 1.603 ± 0.043
0.248HisTrp: 0.248 ± 0.014
0.866HisTyr: 0.866 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.236IleAla: 5.236 ± 0.078
0.514IleCys: 0.514 ± 0.02
4.029IleAsp: 4.029 ± 0.064
5.142IleGlu: 5.142 ± 0.079
2.815IlePhe: 2.815 ± 0.061
5.498IleGly: 5.498 ± 0.087
1.6IleHis: 1.6 ± 0.036
4.715IleIle: 4.715 ± 0.087
3.538IleLys: 3.538 ± 0.067
6.081IleLeu: 6.081 ± 0.085
1.633IleMet: 1.633 ± 0.042
2.629IleAsn: 2.629 ± 0.049
3.093IlePro: 3.093 ± 0.061
2.338IleGln: 2.338 ± 0.044
3.179IleArg: 3.179 ± 0.056
4.238IleSer: 4.238 ± 0.072
4.026IleThr: 4.026 ± 0.063
4.953IleVal: 4.953 ± 0.069
0.6IleTrp: 0.6 ± 0.025
2.106IleTyr: 2.106 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
4.373LysAla: 4.373 ± 0.068
0.286LysCys: 0.286 ± 0.017
3.618LysAsp: 3.618 ± 0.068
6.701LysGlu: 6.701 ± 0.093
1.339LysPhe: 1.339 ± 0.036
4.46LysGly: 4.46 ± 0.074
1.239LysHis: 1.239 ± 0.035
3.236LysIle: 3.236 ± 0.06
5.564LysLys: 5.564 ± 0.085
4.753LysLeu: 4.753 ± 0.075
1.824LysMet: 1.824 ± 0.036
2.586LysAsn: 2.586 ± 0.051
2.194LysPro: 2.194 ± 0.054
2.626LysGln: 2.626 ± 0.055
3.616LysArg: 3.616 ± 0.055
3.138LysSer: 3.138 ± 0.057
3.396LysThr: 3.396 ± 0.054
4.144LysVal: 4.144 ± 0.07
0.739LysTrp: 0.739 ± 0.025
1.672LysTyr: 1.672 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
7.155LeuAla: 7.155 ± 0.087
0.618LeuCys: 0.618 ± 0.025
4.935LeuAsp: 4.935 ± 0.075
6.664LeuGlu: 6.664 ± 0.09
4.925LeuPhe: 4.925 ± 0.095
6.612LeuGly: 6.612 ± 0.093
2.054LeuHis: 2.054 ± 0.048
6.584LeuIle: 6.584 ± 0.102
5.862LeuLys: 5.862 ± 0.078
9.434LeuLeu: 9.434 ± 0.126
2.564LeuMet: 2.564 ± 0.056
3.794LeuAsn: 3.794 ± 0.058
4.024LeuPro: 4.024 ± 0.063
2.98LeuGln: 2.98 ± 0.05
3.925LeuArg: 3.925 ± 0.067
6.677LeuSer: 6.677 ± 0.078
5.977LeuThr: 5.977 ± 0.076
6.435LeuVal: 6.435 ± 0.082
0.812LeuTrp: 0.812 ± 0.031
3.109LeuTyr: 3.109 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.223MetAla: 2.223 ± 0.049
0.155MetCys: 0.155 ± 0.012
1.603MetAsp: 1.603 ± 0.044
2.146MetGlu: 2.146 ± 0.045
1.133MetPhe: 1.133 ± 0.033
1.937MetGly: 1.937 ± 0.04
0.445MetHis: 0.445 ± 0.021
2.194MetIle: 2.194 ± 0.04
2.39MetLys: 2.39 ± 0.051
2.487MetLeu: 2.487 ± 0.052
0.997MetMet: 0.997 ± 0.034
1.587MetAsn: 1.587 ± 0.037
1.042MetPro: 1.042 ± 0.035
0.829MetGln: 0.829 ± 0.03
1.19MetArg: 1.19 ± 0.029
1.764MetSer: 1.764 ± 0.038
1.851MetThr: 1.851 ± 0.035
1.877MetVal: 1.877 ± 0.043
0.254MetTrp: 0.254 ± 0.015
0.766MetTyr: 0.766 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.455AsnAla: 2.455 ± 0.053
0.253AsnCys: 0.253 ± 0.015
2.233AsnAsp: 2.233 ± 0.05
3.428AsnGlu: 3.428 ± 0.059
1.361AsnPhe: 1.361 ± 0.039
3.141AsnGly: 3.141 ± 0.061
0.999AsnHis: 0.999 ± 0.029
2.61AsnIle: 2.61 ± 0.058
2.277AsnLys: 2.277 ± 0.048
3.409AsnLeu: 3.409 ± 0.052
1.117AsnMet: 1.117 ± 0.031
1.634AsnAsn: 1.634 ± 0.044
1.973AsnPro: 1.973 ± 0.042
1.572AsnGln: 1.572 ± 0.037
2.079AsnArg: 2.079 ± 0.046
1.837AsnSer: 1.837 ± 0.046
2.047AsnThr: 2.047 ± 0.045
3.041AsnVal: 3.041 ± 0.051
0.478AsnTrp: 0.478 ± 0.024
1.282AsnTyr: 1.282 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
2.718ProAla: 2.718 ± 0.049
0.227ProCys: 0.227 ± 0.014
2.533ProAsp: 2.533 ± 0.051
3.496ProGlu: 3.496 ± 0.063
2.108ProPhe: 2.108 ± 0.045
2.938ProGly: 2.938 ± 0.063
0.901ProHis: 0.901 ± 0.026
2.054ProIle: 2.054 ± 0.042
1.86ProLys: 1.86 ± 0.041
3.605ProLeu: 3.605 ± 0.065
0.931ProMet: 0.931 ± 0.029
1.309ProAsn: 1.309 ± 0.038
1.172ProPro: 1.172 ± 0.036
1.094ProGln: 1.094 ± 0.033
1.295ProArg: 1.295 ± 0.036
2.317ProSer: 2.317 ± 0.047
1.613ProThr: 1.613 ± 0.038
3.67ProVal: 3.67 ± 0.057
0.437ProTrp: 0.437 ± 0.019
1.413ProTyr: 1.413 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.599GlnAla: 2.599 ± 0.048
0.185GlnCys: 0.185 ± 0.013
1.548GlnAsp: 1.548 ± 0.039
2.765GlnGlu: 2.765 ± 0.053
1.408GlnPhe: 1.408 ± 0.03
2.177GlnGly: 2.177 ± 0.044
0.682GlnHis: 0.682 ± 0.022
2.032GlnIle: 2.032 ± 0.038
2.277GlnLys: 2.277 ± 0.053
3.362GlnLeu: 3.362 ± 0.058
1.169GlnMet: 1.169 ± 0.033
1.372GlnAsn: 1.372 ± 0.033
1.179GlnPro: 1.179 ± 0.035
1.406GlnGln: 1.406 ± 0.051
1.531GlnArg: 1.531 ± 0.038
2.013GlnSer: 2.013 ± 0.041
1.945GlnThr: 1.945 ± 0.045
2.454GlnVal: 2.454 ± 0.053
0.414GlnTrp: 0.414 ± 0.019
1.099GlnTyr: 1.099 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.731ArgAla: 2.731 ± 0.048
0.316ArgCys: 0.316 ± 0.016
2.481ArgAsp: 2.481 ± 0.042
3.868ArgGlu: 3.868 ± 0.058
2.229ArgPhe: 2.229 ± 0.043
2.927ArgGly: 2.927 ± 0.048
1.04ArgHis: 1.04 ± 0.029
3.21ArgIle: 3.21 ± 0.055
3.236ArgLys: 3.236 ± 0.059
4.443ArgLeu: 4.443 ± 0.066
1.568ArgMet: 1.568 ± 0.04
1.935ArgAsn: 1.935 ± 0.037
1.548ArgPro: 1.548 ± 0.036
1.678ArgGln: 1.678 ± 0.043
2.214ArgArg: 2.214 ± 0.055
2.614ArgSer: 2.614 ± 0.049
2.444ArgThr: 2.444 ± 0.041
3.184ArgVal: 3.184 ± 0.058
0.534ArgTrp: 0.534 ± 0.023
1.686ArgTyr: 1.686 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.925SerAla: 3.925 ± 0.055
0.397SerCys: 0.397 ± 0.02
3.098SerAsp: 3.098 ± 0.056
4.302SerGlu: 4.302 ± 0.074
3.396SerPhe: 3.396 ± 0.064
4.875SerGly: 4.875 ± 0.067
1.279SerHis: 1.279 ± 0.032
4.058SerIle: 4.058 ± 0.059
3.119SerLys: 3.119 ± 0.058
6.147SerLeu: 6.147 ± 0.075
1.715SerMet: 1.715 ± 0.04
2.07SerAsn: 2.07 ± 0.046
2.217SerPro: 2.217 ± 0.05
1.876SerGln: 1.876 ± 0.038
2.651SerArg: 2.651 ± 0.054
3.815SerSer: 3.815 ± 0.079
2.951SerThr: 2.951 ± 0.052
4.514SerVal: 4.514 ± 0.067
0.688SerTrp: 0.688 ± 0.027
2.097SerTyr: 2.097 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
4.259ThrAla: 4.259 ± 0.064
0.374ThrCys: 0.374 ± 0.016
3.03ThrAsp: 3.03 ± 0.05
3.763ThrGlu: 3.763 ± 0.064
2.781ThrPhe: 2.781 ± 0.05
4.766ThrGly: 4.766 ± 0.072
1.088ThrHis: 1.088 ± 0.033
4.247ThrIle: 4.247 ± 0.072
2.994ThrLys: 2.994 ± 0.053
5.407ThrLeu: 5.407 ± 0.068
1.436ThrMet: 1.436 ± 0.033
2.097ThrAsn: 2.097 ± 0.046
2.303ThrPro: 2.303 ± 0.053
1.409ThrGln: 1.409 ± 0.039
2.115ThrArg: 2.115 ± 0.041
3.195ThrSer: 3.195 ± 0.052
2.778ThrThr: 2.778 ± 0.054
4.623ThrVal: 4.623 ± 0.069
0.531ThrTrp: 0.531 ± 0.02
1.964ThrTyr: 1.964 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.311ValAla: 5.311 ± 0.087
0.611ValCys: 0.611 ± 0.025
3.944ValAsp: 3.944 ± 0.061
5.241ValGlu: 5.241 ± 0.076
3.455ValPhe: 3.455 ± 0.063
4.452ValGly: 4.452 ± 0.064
1.539ValHis: 1.539 ± 0.04
5.785ValIle: 5.785 ± 0.08
4.637ValLys: 4.637 ± 0.07
7.202ValLeu: 7.202 ± 0.086
2.185ValMet: 2.185 ± 0.044
3.238ValAsn: 3.238 ± 0.058
2.934ValPro: 2.934 ± 0.05
2.357ValGln: 2.357 ± 0.043
3.241ValArg: 3.241 ± 0.057
4.99ValSer: 4.99 ± 0.077
4.754ValThr: 4.754 ± 0.065
5.447ValVal: 5.447 ± 0.08
0.74ValTrp: 0.74 ± 0.027
2.498ValTyr: 2.498 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.682TrpAla: 0.682 ± 0.026
0.073TrpCys: 0.073 ± 0.007
0.617TrpAsp: 0.617 ± 0.024
0.771TrpGlu: 0.771 ± 0.025
0.547TrpPhe: 0.547 ± 0.023
0.738TrpGly: 0.738 ± 0.026
0.246TrpHis: 0.246 ± 0.015
0.86TrpIle: 0.86 ± 0.029
0.685TrpLys: 0.685 ± 0.027
1.205TrpLeu: 1.205 ± 0.035
0.404TrpMet: 0.404 ± 0.017
0.495TrpAsn: 0.495 ± 0.02
0.329TrpPro: 0.329 ± 0.019
0.42TrpGln: 0.42 ± 0.019
0.417TrpArg: 0.417 ± 0.019
0.6TrpSer: 0.6 ± 0.024
0.555TrpThr: 0.555 ± 0.022
0.72TrpVal: 0.72 ± 0.027
0.138TrpTrp: 0.138 ± 0.011
0.375TrpTyr: 0.375 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.084TyrAla: 2.084 ± 0.042
0.272TyrCys: 0.272 ± 0.016
2.041TyrAsp: 2.041 ± 0.043
2.765TyrGlu: 2.765 ± 0.057
1.754TyrPhe: 1.754 ± 0.041
2.52TyrGly: 2.52 ± 0.05
0.868TyrHis: 0.868 ± 0.03
2.027TyrIle: 2.027 ± 0.042
1.714TyrLys: 1.714 ± 0.042
3.352TyrLeu: 3.352 ± 0.06
0.871TyrMet: 0.871 ± 0.027
1.316TyrAsn: 1.316 ± 0.036
1.42TyrPro: 1.42 ± 0.04
1.204TyrGln: 1.204 ± 0.029
1.735TyrArg: 1.735 ± 0.041
2.005TyrSer: 2.005 ± 0.039
1.81TyrThr: 1.81 ± 0.038
2.294TyrVal: 2.294 ± 0.046
0.386TyrTrp: 0.386 ± 0.02
1.337TyrTyr: 1.337 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3944 proteins (1152186 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski