Gordonia phage Vasanti

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Attisvirus; unclassified Attisvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BVW6|A0A411BVW6_9CAUD Major tail protein OS=Gordonia phage Vasanti OX=2502431 GN=12 PE=4 SV=1
MM1 pKa = 7.72SYY3 pKa = 9.13TVTAALVVAADD14 pKa = 3.95TEE16 pKa = 4.72GKK18 pKa = 10.42LNYY21 pKa = 9.23HH22 pKa = 6.06YY23 pKa = 10.34EE24 pKa = 4.39GAHH27 pKa = 5.63IAYY30 pKa = 10.27LSDD33 pKa = 3.19EE34 pKa = 4.42DD35 pKa = 4.66ADD37 pKa = 4.52RR38 pKa = 11.84FLADD42 pKa = 3.3GMVVEE47 pKa = 4.81TADD50 pKa = 3.93LADD53 pKa = 3.5EE54 pKa = 4.42AFIPFADD61 pKa = 4.62DD62 pKa = 3.67PADD65 pKa = 4.12GGDD68 pKa = 4.31DD69 pKa = 3.84PVPPAGDD76 pKa = 3.35GARR79 pKa = 11.84PPKK82 pKa = 9.6TASKK86 pKa = 10.3DD87 pKa = 3.08AWVDD91 pKa = 3.79FAEE94 pKa = 4.47AKK96 pKa = 10.36GMSRR100 pKa = 11.84AEE102 pKa = 4.1AEE104 pKa = 4.46DD105 pKa = 3.62MSKK108 pKa = 11.33ADD110 pKa = 5.46LIQALGG116 pKa = 3.34

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BW15|A0A411BW15_9CAUD Uncharacterized protein OS=Gordonia phage Vasanti OX=2502431 GN=63 PE=4 SV=1
MM1 pKa = 7.69CPACYY6 pKa = 9.96EE7 pKa = 3.97RR8 pKa = 11.84DD9 pKa = 3.31RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84QKK14 pKa = 11.14AYY16 pKa = 10.35GRR18 pKa = 11.84WEE20 pKa = 4.2SQHH23 pKa = 7.67VEE25 pKa = 3.89AQPVRR30 pKa = 11.84DD31 pKa = 4.37HH32 pKa = 6.95IEE34 pKa = 3.88KK35 pKa = 10.65LRR37 pKa = 11.84GAGISNSRR45 pKa = 11.84LRR47 pKa = 11.84EE48 pKa = 4.01LTGVSHH54 pKa = 6.05NTIQVLMTGRR64 pKa = 11.84PEE66 pKa = 4.34RR67 pKa = 11.84GHH69 pKa = 6.65GPTKK73 pKa = 10.23KK74 pKa = 10.16VLRR77 pKa = 11.84RR78 pKa = 11.84TADD81 pKa = 3.96RR82 pKa = 11.84ILAVPVPEE90 pKa = 4.54LAFTVASPGRR100 pKa = 11.84IVPALGTTRR109 pKa = 11.84RR110 pKa = 11.84LQALVANGYY119 pKa = 7.26SQRR122 pKa = 11.84EE123 pKa = 4.08LCRR126 pKa = 11.84RR127 pKa = 11.84LGWVWQGNATALFLGRR143 pKa = 11.84AEE145 pKa = 4.05YY146 pKa = 10.67VVARR150 pKa = 11.84RR151 pKa = 11.84ARR153 pKa = 11.84EE154 pKa = 3.82VADD157 pKa = 5.64LFTQLQLVPGTDD169 pKa = 2.81VRR171 pKa = 11.84ARR173 pKa = 11.84NRR175 pKa = 11.84ARR177 pKa = 11.84ANGWPAPLDD186 pKa = 3.55WDD188 pKa = 4.36EE189 pKa = 4.84DD190 pKa = 4.46TIDD193 pKa = 5.41DD194 pKa = 4.64PDD196 pKa = 4.01HH197 pKa = 6.76VPTLAADD204 pKa = 4.29PPRR207 pKa = 11.84MSTTLDD213 pKa = 3.15EE214 pKa = 5.34FEE216 pKa = 4.81WLLQCGEE223 pKa = 4.17IPEE226 pKa = 4.35VAAKK230 pKa = 10.22RR231 pKa = 11.84CGVRR235 pKa = 11.84LGTIRR240 pKa = 11.84IYY242 pKa = 9.45YY243 pKa = 9.04ARR245 pKa = 11.84EE246 pKa = 3.72GRR248 pKa = 11.84EE249 pKa = 4.15VPHH252 pKa = 6.29QLAPPSPQPVAHH264 pKa = 6.5PRR266 pKa = 11.84HH267 pKa = 6.5RR268 pKa = 11.84KK269 pKa = 6.3TQVASS274 pKa = 3.48

Molecular weight: 30.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14881

26

1448

209.6

22.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.419 ± 0.498

0.813 ± 0.168

6.901 ± 0.266

5.974 ± 0.276

2.634 ± 0.154

8.413 ± 0.433

1.868 ± 0.186

4.758 ± 0.162

2.762 ± 0.189

7.667 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.14

2.99 ± 0.191

5.645 ± 0.18

3.588 ± 0.295

7.741 ± 0.378

5.557 ± 0.192

6.881 ± 0.33

7.224 ± 0.376

2.003 ± 0.105

2.056 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski