Amino acid dipepetide frequency for Gordonia phage Vasanti

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.347AlaAla: 18.347 ± 1.624
1.075AlaCys: 1.075 ± 0.295
9.61AlaAsp: 9.61 ± 0.929
8.871AlaGlu: 8.871 ± 0.911
2.419AlaPhe: 2.419 ± 0.402
9.543AlaGly: 9.543 ± 0.957
1.949AlaHis: 1.949 ± 0.371
5.981AlaIle: 5.981 ± 0.577
3.831AlaLys: 3.831 ± 0.597
9.745AlaLeu: 9.745 ± 0.792
3.965AlaMet: 3.965 ± 0.518
4.57AlaAsn: 4.57 ± 0.654
5.914AlaPro: 5.914 ± 0.795
4.301AlaGln: 4.301 ± 0.506
8.401AlaArg: 8.401 ± 0.899
6.183AlaSer: 6.183 ± 0.629
8.065AlaThr: 8.065 ± 1.189
7.527AlaVal: 7.527 ± 0.908
1.546AlaTrp: 1.546 ± 0.27
2.285AlaTyr: 2.285 ± 0.347
0.0AlaXaa: 0.0 ± 0.0
Cys
0.739CysAla: 0.739 ± 0.231
0.134CysCys: 0.134 ± 0.101
0.605CysAsp: 0.605 ± 0.235
0.47CysGlu: 0.47 ± 0.18
0.134CysPhe: 0.134 ± 0.094
1.21CysGly: 1.21 ± 0.37
0.067CysHis: 0.067 ± 0.06
0.336CysIle: 0.336 ± 0.15
0.336CysLys: 0.336 ± 0.14
0.336CysLeu: 0.336 ± 0.136
0.0CysMet: 0.0 ± 0.0
0.202CysAsn: 0.202 ± 0.131
0.605CysPro: 0.605 ± 0.23
0.202CysGln: 0.202 ± 0.124
0.739CysArg: 0.739 ± 0.207
0.605CysSer: 0.605 ± 0.236
0.47CysThr: 0.47 ± 0.189
0.605CysVal: 0.605 ± 0.203
0.067CysTrp: 0.067 ± 0.086
0.269CysTyr: 0.269 ± 0.156
0.0CysXaa: 0.0 ± 0.0
Asp
7.392AspAla: 7.392 ± 0.767
0.202AspCys: 0.202 ± 0.108
5.712AspAsp: 5.712 ± 0.891
4.704AspGlu: 4.704 ± 0.604
2.285AspPhe: 2.285 ± 0.348
6.452AspGly: 6.452 ± 0.541
1.747AspHis: 1.747 ± 0.319
2.285AspIle: 2.285 ± 0.507
1.21AspLys: 1.21 ± 0.258
6.25AspLeu: 6.25 ± 0.576
1.21AspMet: 1.21 ± 0.3
1.747AspAsn: 1.747 ± 0.406
5.578AspPro: 5.578 ± 0.682
2.083AspGln: 2.083 ± 0.402
4.839AspArg: 4.839 ± 0.594
3.159AspSer: 3.159 ± 0.501
3.427AspThr: 3.427 ± 0.426
5.242AspVal: 5.242 ± 0.538
1.815AspTrp: 1.815 ± 0.367
1.68AspTyr: 1.68 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
7.325GluAla: 7.325 ± 0.711
0.605GluCys: 0.605 ± 0.207
2.957GluAsp: 2.957 ± 0.402
2.823GluGlu: 2.823 ± 0.597
1.949GluPhe: 1.949 ± 0.328
3.898GluGly: 3.898 ± 0.513
1.882GluHis: 1.882 ± 0.307
3.36GluIle: 3.36 ± 0.444
1.949GluLys: 1.949 ± 0.336
5.108GluLeu: 5.108 ± 0.734
1.411GluMet: 1.411 ± 0.311
1.277GluAsn: 1.277 ± 0.225
3.562GluPro: 3.562 ± 0.455
3.293GluGln: 3.293 ± 0.531
4.704GluArg: 4.704 ± 0.618
2.89GluSer: 2.89 ± 0.449
3.427GluThr: 3.427 ± 0.463
4.301GluVal: 4.301 ± 0.575
1.21GluTrp: 1.21 ± 0.313
1.815GluTyr: 1.815 ± 0.286
0.0GluXaa: 0.0 ± 0.0
Phe
3.696PheAla: 3.696 ± 0.484
0.202PheCys: 0.202 ± 0.107
2.419PheAsp: 2.419 ± 0.393
1.613PheGlu: 1.613 ± 0.344
0.806PhePhe: 0.806 ± 0.233
3.091PheGly: 3.091 ± 0.513
0.336PheHis: 0.336 ± 0.144
0.941PheIle: 0.941 ± 0.235
0.605PheLys: 0.605 ± 0.204
1.344PheLeu: 1.344 ± 0.272
0.605PheMet: 0.605 ± 0.18
0.739PheAsn: 0.739 ± 0.191
1.21PhePro: 1.21 ± 0.227
0.739PheGln: 0.739 ± 0.208
1.613PheArg: 1.613 ± 0.317
1.142PheSer: 1.142 ± 0.224
2.352PheThr: 2.352 ± 0.419
1.68PheVal: 1.68 ± 0.373
1.008PheTrp: 1.008 ± 0.296
0.202PheTyr: 0.202 ± 0.119
0.0PheXaa: 0.0 ± 0.0
Gly
8.602GlyAla: 8.602 ± 1.003
0.336GlyCys: 0.336 ± 0.306
5.981GlyAsp: 5.981 ± 0.626
5.511GlyGlu: 5.511 ± 0.514
1.68GlyPhe: 1.68 ± 0.426
7.527GlyGly: 7.527 ± 1.286
1.411GlyHis: 1.411 ± 0.255
3.831GlyIle: 3.831 ± 0.674
2.487GlyLys: 2.487 ± 0.446
7.124GlyLeu: 7.124 ± 0.748
1.882GlyMet: 1.882 ± 0.362
2.487GlyAsn: 2.487 ± 0.42
4.167GlyPro: 4.167 ± 0.53
2.621GlyGln: 2.621 ± 0.482
6.855GlyArg: 6.855 ± 0.658
5.511GlySer: 5.511 ± 0.725
5.242GlyThr: 5.242 ± 0.593
6.855GlyVal: 6.855 ± 0.703
1.747GlyTrp: 1.747 ± 0.439
2.285GlyTyr: 2.285 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.882HisAla: 1.882 ± 0.321
0.269HisCys: 0.269 ± 0.13
1.411HisAsp: 1.411 ± 0.293
0.739HisGlu: 0.739 ± 0.309
0.47HisPhe: 0.47 ± 0.157
1.21HisGly: 1.21 ± 0.34
0.47HisHis: 0.47 ± 0.165
0.806HisIle: 0.806 ± 0.26
0.336HisLys: 0.336 ± 0.155
1.344HisLeu: 1.344 ± 0.293
0.202HisMet: 0.202 ± 0.086
0.538HisAsn: 0.538 ± 0.172
1.478HisPro: 1.478 ± 0.332
1.075HisGln: 1.075 ± 0.279
1.478HisArg: 1.478 ± 0.301
1.075HisSer: 1.075 ± 0.286
1.344HisThr: 1.344 ± 0.28
1.546HisVal: 1.546 ± 0.362
0.538HisTrp: 0.538 ± 0.195
0.47HisTyr: 0.47 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
6.855IleAla: 6.855 ± 0.613
0.336IleCys: 0.336 ± 0.144
3.831IleAsp: 3.831 ± 0.556
3.091IleGlu: 3.091 ± 0.45
1.277IlePhe: 1.277 ± 0.377
4.301IleGly: 4.301 ± 0.606
0.874IleHis: 0.874 ± 0.257
1.478IleIle: 1.478 ± 0.338
1.075IleLys: 1.075 ± 0.264
2.957IleLeu: 2.957 ± 0.406
0.403IleMet: 0.403 ± 0.158
1.411IleAsn: 1.411 ± 0.348
2.554IlePro: 2.554 ± 0.408
2.218IleGln: 2.218 ± 0.324
3.629IleArg: 3.629 ± 0.362
2.755IleSer: 2.755 ± 0.365
3.024IleThr: 3.024 ± 0.548
3.898IleVal: 3.898 ± 0.425
0.269IleTrp: 0.269 ± 0.131
1.344IleTyr: 1.344 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
2.823LysAla: 2.823 ± 0.411
0.134LysCys: 0.134 ± 0.098
1.882LysAsp: 1.882 ± 0.327
1.546LysGlu: 1.546 ± 0.352
0.336LysPhe: 0.336 ± 0.148
2.083LysGly: 2.083 ± 0.518
0.336LysHis: 0.336 ± 0.131
1.478LysIle: 1.478 ± 0.369
0.806LysLys: 0.806 ± 0.26
2.554LysLeu: 2.554 ± 0.465
0.806LysMet: 0.806 ± 0.252
0.739LysAsn: 0.739 ± 0.227
1.747LysPro: 1.747 ± 0.327
1.075LysGln: 1.075 ± 0.294
2.419LysArg: 2.419 ± 0.482
1.613LysSer: 1.613 ± 0.343
2.151LysThr: 2.151 ± 0.529
2.083LysVal: 2.083 ± 0.376
0.403LysTrp: 0.403 ± 0.224
0.605LysTyr: 0.605 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
10.753LeuAla: 10.753 ± 0.841
0.605LeuCys: 0.605 ± 0.231
6.116LeuAsp: 6.116 ± 0.618
4.301LeuGlu: 4.301 ± 0.451
2.151LeuPhe: 2.151 ± 0.47
7.325LeuGly: 7.325 ± 0.726
0.806LeuHis: 0.806 ± 0.189
3.091LeuIle: 3.091 ± 0.467
1.613LeuLys: 1.613 ± 0.402
5.981LeuLeu: 5.981 ± 0.891
1.344LeuMet: 1.344 ± 0.308
2.285LeuAsn: 2.285 ± 0.428
3.965LeuPro: 3.965 ± 0.504
1.882LeuGln: 1.882 ± 0.261
6.452LeuArg: 6.452 ± 0.828
4.704LeuSer: 4.704 ± 0.482
5.242LeuThr: 5.242 ± 0.783
5.376LeuVal: 5.376 ± 0.697
1.478LeuTrp: 1.478 ± 0.284
1.21LeuTyr: 1.21 ± 0.319
0.0LeuXaa: 0.0 ± 0.0
Met
3.562MetAla: 3.562 ± 0.636
0.269MetCys: 0.269 ± 0.138
0.806MetAsp: 0.806 ± 0.235
0.672MetGlu: 0.672 ± 0.187
0.605MetPhe: 0.605 ± 0.193
1.613MetGly: 1.613 ± 0.326
0.403MetHis: 0.403 ± 0.153
1.008MetIle: 1.008 ± 0.251
0.202MetLys: 0.202 ± 0.119
1.882MetLeu: 1.882 ± 0.343
0.336MetMet: 0.336 ± 0.166
0.739MetAsn: 0.739 ± 0.201
1.478MetPro: 1.478 ± 0.318
0.47MetGln: 0.47 ± 0.182
1.21MetArg: 1.21 ± 0.247
1.344MetSer: 1.344 ± 0.276
2.688MetThr: 2.688 ± 0.499
1.21MetVal: 1.21 ± 0.261
0.336MetTrp: 0.336 ± 0.148
0.202MetTyr: 0.202 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
3.898AsnAla: 3.898 ± 0.531
0.134AsnCys: 0.134 ± 0.105
1.815AsnAsp: 1.815 ± 0.345
1.613AsnGlu: 1.613 ± 0.285
0.806AsnPhe: 0.806 ± 0.324
3.091AsnGly: 3.091 ± 0.45
1.008AsnHis: 1.008 ± 0.329
1.411AsnIle: 1.411 ± 0.284
0.806AsnLys: 0.806 ± 0.27
1.949AsnLeu: 1.949 ± 0.322
0.538AsnMet: 0.538 ± 0.218
0.538AsnAsn: 0.538 ± 0.154
2.016AsnPro: 2.016 ± 0.432
1.21AsnGln: 1.21 ± 0.233
2.151AsnArg: 2.151 ± 0.41
1.68AsnSer: 1.68 ± 0.333
2.083AsnThr: 2.083 ± 0.404
1.882AsnVal: 1.882 ± 0.426
0.672AsnTrp: 0.672 ± 0.201
0.605AsnTyr: 0.605 ± 0.168
0.0AsnXaa: 0.0 ± 0.0
Pro
7.796ProAla: 7.796 ± 0.959
0.47ProCys: 0.47 ± 0.197
4.301ProAsp: 4.301 ± 0.626
4.099ProGlu: 4.099 ± 0.613
1.344ProPhe: 1.344 ± 0.237
5.04ProGly: 5.04 ± 0.665
0.874ProHis: 0.874 ± 0.245
1.815ProIle: 1.815 ± 0.318
1.68ProLys: 1.68 ± 0.301
2.688ProLeu: 2.688 ± 0.328
1.075ProMet: 1.075 ± 0.245
2.285ProAsn: 2.285 ± 0.362
4.301ProPro: 4.301 ± 0.56
1.815ProGln: 1.815 ± 0.265
3.763ProArg: 3.763 ± 0.612
2.554ProSer: 2.554 ± 0.364
4.167ProThr: 4.167 ± 0.659
4.167ProVal: 4.167 ± 0.6
1.142ProTrp: 1.142 ± 0.265
1.075ProTyr: 1.075 ± 0.257
0.0ProXaa: 0.0 ± 0.0
Gln
3.629GlnAla: 3.629 ± 0.5
0.269GlnCys: 0.269 ± 0.183
1.411GlnAsp: 1.411 ± 0.305
1.747GlnGlu: 1.747 ± 0.362
0.672GlnPhe: 0.672 ± 0.246
1.949GlnGly: 1.949 ± 0.411
0.806GlnHis: 0.806 ± 0.267
2.419GlnIle: 2.419 ± 0.455
1.21GlnLys: 1.21 ± 0.362
3.562GlnLeu: 3.562 ± 0.501
1.075GlnMet: 1.075 ± 0.253
0.605GlnAsn: 0.605 ± 0.209
1.882GlnPro: 1.882 ± 0.334
1.747GlnGln: 1.747 ± 0.398
3.36GlnArg: 3.36 ± 0.561
2.218GlnSer: 2.218 ± 0.319
2.89GlnThr: 2.89 ± 0.403
2.755GlnVal: 2.755 ± 0.345
0.672GlnTrp: 0.672 ± 0.187
1.008GlnTyr: 1.008 ± 0.324
0.0GlnXaa: 0.0 ± 0.0
Arg
9.005ArgAla: 9.005 ± 0.77
1.142ArgCys: 1.142 ± 0.408
4.234ArgAsp: 4.234 ± 0.532
4.906ArgGlu: 4.906 ± 0.7
2.621ArgPhe: 2.621 ± 0.443
4.839ArgGly: 4.839 ± 0.594
1.949ArgHis: 1.949 ± 0.364
4.973ArgIle: 4.973 ± 0.59
2.688ArgLys: 2.688 ± 0.5
5.376ArgLeu: 5.376 ± 0.736
1.815ArgMet: 1.815 ± 0.371
3.226ArgAsn: 3.226 ± 0.557
3.965ArgPro: 3.965 ± 0.515
2.688ArgGln: 2.688 ± 0.378
7.796ArgArg: 7.796 ± 1.024
3.763ArgSer: 3.763 ± 0.591
4.503ArgThr: 4.503 ± 0.494
4.57ArgVal: 4.57 ± 0.43
1.546ArgTrp: 1.546 ± 0.365
1.815ArgTyr: 1.815 ± 0.323
0.0ArgXaa: 0.0 ± 0.0
Ser
6.72SerAla: 6.72 ± 0.847
0.336SerCys: 0.336 ± 0.142
2.487SerAsp: 2.487 ± 0.396
2.823SerGlu: 2.823 ± 0.494
2.016SerPhe: 2.016 ± 0.346
6.183SerGly: 6.183 ± 0.674
0.605SerHis: 0.605 ± 0.212
3.562SerIle: 3.562 ± 0.495
1.747SerLys: 1.747 ± 0.331
3.629SerLeu: 3.629 ± 0.496
1.008SerMet: 1.008 ± 0.274
1.68SerAsn: 1.68 ± 0.389
2.823SerPro: 2.823 ± 0.462
2.016SerGln: 2.016 ± 0.4
4.099SerArg: 4.099 ± 0.572
3.091SerSer: 3.091 ± 0.438
4.301SerThr: 4.301 ± 0.519
3.562SerVal: 3.562 ± 0.474
1.411SerTrp: 1.411 ± 0.292
1.478SerTyr: 1.478 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
8.333ThrAla: 8.333 ± 1.036
0.538ThrCys: 0.538 ± 0.203
4.772ThrAsp: 4.772 ± 0.519
2.823ThrGlu: 2.823 ± 0.521
1.344ThrPhe: 1.344 ± 0.324
6.586ThrGly: 6.586 ± 0.682
1.008ThrHis: 1.008 ± 0.291
4.637ThrIle: 4.637 ± 0.574
1.478ThrLys: 1.478 ± 0.294
6.384ThrLeu: 6.384 ± 0.481
1.008ThrMet: 1.008 ± 0.236
1.747ThrAsn: 1.747 ± 0.411
3.629ThrPro: 3.629 ± 0.489
2.352ThrGln: 2.352 ± 0.494
4.368ThrArg: 4.368 ± 0.509
4.368ThrSer: 4.368 ± 0.629
5.309ThrThr: 5.309 ± 0.823
5.645ThrVal: 5.645 ± 0.782
1.68ThrTrp: 1.68 ± 0.359
0.806ThrTyr: 0.806 ± 0.169
0.0ThrXaa: 0.0 ± 0.0
Val
8.468ValAla: 8.468 ± 0.885
0.739ValCys: 0.739 ± 0.224
5.914ValAsp: 5.914 ± 0.527
5.376ValGlu: 5.376 ± 0.541
2.151ValPhe: 2.151 ± 0.47
4.704ValGly: 4.704 ± 0.634
1.075ValHis: 1.075 ± 0.267
2.688ValIle: 2.688 ± 0.474
1.815ValLys: 1.815 ± 0.376
4.973ValLeu: 4.973 ± 0.661
1.747ValMet: 1.747 ± 0.336
1.882ValAsn: 1.882 ± 0.378
3.562ValPro: 3.562 ± 0.682
2.218ValGln: 2.218 ± 0.447
6.317ValArg: 6.317 ± 0.856
4.167ValSer: 4.167 ± 0.56
5.712ValThr: 5.712 ± 0.75
6.452ValVal: 6.452 ± 1.075
1.411ValTrp: 1.411 ± 0.291
0.874ValTyr: 0.874 ± 0.218
0.0ValXaa: 0.0 ± 0.0
Trp
1.815TrpAla: 1.815 ± 0.36
0.067TrpCys: 0.067 ± 0.086
1.478TrpAsp: 1.478 ± 0.286
1.21TrpGlu: 1.21 ± 0.198
0.739TrpPhe: 0.739 ± 0.243
1.411TrpGly: 1.411 ± 0.327
0.47TrpHis: 0.47 ± 0.168
0.739TrpIle: 0.739 ± 0.197
0.672TrpLys: 0.672 ± 0.231
2.487TrpLeu: 2.487 ± 0.404
0.336TrpMet: 0.336 ± 0.155
0.605TrpAsn: 0.605 ± 0.186
1.075TrpPro: 1.075 ± 0.284
0.941TrpGln: 0.941 ± 0.221
1.815TrpArg: 1.815 ± 0.32
1.411TrpSer: 1.411 ± 0.298
1.142TrpThr: 1.142 ± 0.312
1.008TrpVal: 1.008 ± 0.224
0.403TrpTrp: 0.403 ± 0.141
0.202TrpTyr: 0.202 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.554TyrAla: 2.554 ± 0.464
0.269TyrCys: 0.269 ± 0.118
1.277TyrAsp: 1.277 ± 0.243
0.806TyrGlu: 0.806 ± 0.225
0.538TyrPhe: 0.538 ± 0.162
2.083TyrGly: 2.083 ± 0.443
0.605TyrHis: 0.605 ± 0.174
0.739TyrIle: 0.739 ± 0.194
1.075TyrLys: 1.075 ± 0.248
1.008TyrLeu: 1.008 ± 0.23
0.202TyrMet: 0.202 ± 0.087
0.605TyrAsn: 0.605 ± 0.192
0.941TyrPro: 0.941 ± 0.289
0.941TyrGln: 0.941 ± 0.241
1.411TyrArg: 1.411 ± 0.362
1.344TyrSer: 1.344 ± 0.209
1.277TyrThr: 1.277 ± 0.256
1.882TyrVal: 1.882 ± 0.294
0.672TyrTrp: 0.672 ± 0.201
0.336TyrTyr: 0.336 ± 0.143
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (14881 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski