Ralstonia phage Hyacinthe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Rahariannevirus; unclassified Rahariannevirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5BB34|A0A7G5BB34_9CAUD Uncharacterized protein OS=Ralstonia phage Hyacinthe OX=2759731 GN=8G_00075 PE=4 SV=1
MM1 pKa = 7.92AYY3 pKa = 8.35YY4 pKa = 9.84TLTIADD10 pKa = 4.18SGADD14 pKa = 3.25DD15 pKa = 4.36DD16 pKa = 4.98EE17 pKa = 5.24IRR19 pKa = 11.84EE20 pKa = 4.37GVHH23 pKa = 6.24AAAALLAARR32 pKa = 11.84DD33 pKa = 3.67ISVEE37 pKa = 3.94QAHH40 pKa = 6.61IASVLRR46 pKa = 11.84SCGDD50 pKa = 3.17TYY52 pKa = 11.57DD53 pKa = 4.74ADD55 pKa = 4.82AAQAWDD61 pKa = 3.8DD62 pKa = 3.61AEE64 pKa = 4.28YY65 pKa = 10.92AAFRR69 pKa = 11.84AAFLTWHH76 pKa = 6.53SWPEE80 pKa = 3.84SAALAVAA87 pKa = 4.69

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5BB32|A0A7G5BB32_9CAUD Uncharacterized protein OS=Ralstonia phage Hyacinthe OX=2759731 GN=8G_00073 PE=4 SV=1
MM1 pKa = 5.82TTARR5 pKa = 11.84IPPTAQQVRR14 pKa = 11.84EE15 pKa = 4.07ARR17 pKa = 11.84EE18 pKa = 3.95AAGLTQTEE26 pKa = 4.23AGALVYY32 pKa = 10.52SALRR36 pKa = 11.84AWQQWEE42 pKa = 3.78AGDD45 pKa = 4.36RR46 pKa = 11.84RR47 pKa = 11.84MHH49 pKa = 6.11PALWEE54 pKa = 3.86LFRR57 pKa = 11.84IKK59 pKa = 10.1IIMRR63 pKa = 11.84VTHH66 pKa = 6.07NN67 pKa = 3.45

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

13878

30

636

185.0

20.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.725 ± 0.393

1.146 ± 0.169

5.368 ± 0.26

4.806 ± 0.431

3.055 ± 0.18

8.186 ± 0.434

1.751 ± 0.17

4.763 ± 0.199

4.259 ± 0.337

7.717 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.147

3.985 ± 0.227

4.359 ± 0.228

4.857 ± 0.246

5.584 ± 0.367

6.391 ± 0.325

6.636 ± 0.461

7.249 ± 0.342

1.556 ± 0.12

3.062 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski