Amino acid dipepetide frequency for Ralstonia phage Hyacinthe

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.24AlaAla: 19.24 ± 1.462
1.153AlaCys: 1.153 ± 0.287
7.422AlaAsp: 7.422 ± 0.778
6.774AlaGlu: 6.774 ± 1.027
3.459AlaPhe: 3.459 ± 0.598
9.8AlaGly: 9.8 ± 0.951
1.441AlaHis: 1.441 ± 0.297
5.765AlaIle: 5.765 ± 0.471
5.981AlaLys: 5.981 ± 0.811
11.674AlaLeu: 11.674 ± 0.9
3.603AlaMet: 3.603 ± 0.537
4.612AlaAsn: 4.612 ± 0.579
4.035AlaPro: 4.035 ± 0.6
5.981AlaGln: 5.981 ± 0.716
5.621AlaArg: 5.621 ± 0.664
8.719AlaSer: 8.719 ± 1.109
7.711AlaThr: 7.711 ± 1.12
9.008AlaVal: 9.008 ± 1.009
1.802AlaTrp: 1.802 ± 0.354
3.459AlaTyr: 3.459 ± 0.542
0.0AlaXaa: 0.0 ± 0.0
Cys
1.081CysAla: 1.081 ± 0.291
0.432CysCys: 0.432 ± 0.233
0.649CysAsp: 0.649 ± 0.315
0.937CysGlu: 0.937 ± 0.302
0.072CysPhe: 0.072 ± 0.063
1.153CysGly: 1.153 ± 0.329
0.288CysHis: 0.288 ± 0.167
0.216CysIle: 0.216 ± 0.121
1.153CysLys: 1.153 ± 0.363
0.504CysLeu: 0.504 ± 0.194
0.216CysMet: 0.216 ± 0.138
0.576CysAsn: 0.576 ± 0.172
0.36CysPro: 0.36 ± 0.153
0.36CysGln: 0.36 ± 0.158
1.081CysArg: 1.081 ± 0.295
0.649CysSer: 0.649 ± 0.273
0.576CysThr: 0.576 ± 0.24
0.36CysVal: 0.36 ± 0.152
0.216CysTrp: 0.216 ± 0.148
0.576CysTyr: 0.576 ± 0.376
0.0CysXaa: 0.0 ± 0.0
Asp
6.99AspAla: 6.99 ± 0.725
0.576AspCys: 0.576 ± 0.196
3.747AspAsp: 3.747 ± 0.623
3.171AspGlu: 3.171 ± 0.622
2.234AspPhe: 2.234 ± 0.381
4.828AspGly: 4.828 ± 0.577
1.153AspHis: 1.153 ± 0.335
2.45AspIle: 2.45 ± 0.471
1.729AspLys: 1.729 ± 0.419
4.972AspLeu: 4.972 ± 0.635
1.225AspMet: 1.225 ± 0.297
1.657AspAsn: 1.657 ± 0.345
2.594AspPro: 2.594 ± 0.462
2.234AspGln: 2.234 ± 0.405
2.522AspArg: 2.522 ± 0.328
3.099AspSer: 3.099 ± 0.411
2.738AspThr: 2.738 ± 0.471
3.315AspVal: 3.315 ± 0.448
0.865AspTrp: 0.865 ± 0.216
1.585AspTyr: 1.585 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
5.981GluAla: 5.981 ± 0.858
0.432GluCys: 0.432 ± 0.167
1.657GluAsp: 1.657 ± 0.375
2.522GluGlu: 2.522 ± 0.624
1.369GluPhe: 1.369 ± 0.304
2.955GluGly: 2.955 ± 0.531
1.369GluHis: 1.369 ± 0.34
3.171GluIle: 3.171 ± 0.538
2.09GluLys: 2.09 ± 0.488
3.603GluLeu: 3.603 ± 0.556
1.369GluMet: 1.369 ± 0.327
1.513GluAsn: 1.513 ± 0.359
1.802GluPro: 1.802 ± 0.361
2.594GluGln: 2.594 ± 0.464
3.027GluArg: 3.027 ± 0.563
2.882GluSer: 2.882 ± 0.54
2.378GluThr: 2.378 ± 0.443
3.531GluVal: 3.531 ± 0.538
1.441GluTrp: 1.441 ± 0.338
2.378GluTyr: 2.378 ± 0.452
0.0GluXaa: 0.0 ± 0.0
Phe
3.963PheAla: 3.963 ± 0.593
0.288PheCys: 0.288 ± 0.14
2.162PheAsp: 2.162 ± 0.333
1.153PheGlu: 1.153 ± 0.336
1.081PhePhe: 1.081 ± 0.315
2.955PheGly: 2.955 ± 0.435
0.865PheHis: 0.865 ± 0.28
0.649PheIle: 0.649 ± 0.225
1.585PheLys: 1.585 ± 0.377
1.585PheLeu: 1.585 ± 0.325
0.649PheMet: 0.649 ± 0.223
1.225PheAsn: 1.225 ± 0.293
1.585PhePro: 1.585 ± 0.383
1.297PheGln: 1.297 ± 0.35
2.162PheArg: 2.162 ± 0.456
2.018PheSer: 2.018 ± 0.419
1.369PheThr: 1.369 ± 0.351
2.594PheVal: 2.594 ± 0.386
0.504PheTrp: 0.504 ± 0.216
0.865PheTyr: 0.865 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
10.089GlyAla: 10.089 ± 1.119
0.865GlyCys: 0.865 ± 0.247
3.531GlyAsp: 3.531 ± 0.495
3.171GlyGlu: 3.171 ± 0.606
2.666GlyPhe: 2.666 ± 0.546
7.927GlyGly: 7.927 ± 1.089
1.009GlyHis: 1.009 ± 0.315
4.396GlyIle: 4.396 ± 0.543
3.459GlyLys: 3.459 ± 0.486
6.413GlyLeu: 6.413 ± 0.621
2.738GlyMet: 2.738 ± 0.424
3.315GlyAsn: 3.315 ± 0.573
2.018GlyPro: 2.018 ± 0.389
3.675GlyGln: 3.675 ± 0.489
3.747GlyArg: 3.747 ± 0.631
4.612GlySer: 4.612 ± 0.954
6.99GlyThr: 6.99 ± 1.107
6.125GlyVal: 6.125 ± 0.675
1.585GlyTrp: 1.585 ± 0.313
3.531GlyTyr: 3.531 ± 0.689
0.0GlyXaa: 0.0 ± 0.0
His
1.874HisAla: 1.874 ± 0.382
0.36HisCys: 0.36 ± 0.17
0.937HisAsp: 0.937 ± 0.238
0.865HisGlu: 0.865 ± 0.285
0.576HisPhe: 0.576 ± 0.197
1.225HisGly: 1.225 ± 0.348
0.576HisHis: 0.576 ± 0.228
1.153HisIle: 1.153 ± 0.339
0.937HisLys: 0.937 ± 0.314
1.081HisLeu: 1.081 ± 0.249
0.432HisMet: 0.432 ± 0.219
0.865HisAsn: 0.865 ± 0.303
1.081HisPro: 1.081 ± 0.269
0.504HisGln: 0.504 ± 0.196
1.153HisArg: 1.153 ± 0.28
1.225HisSer: 1.225 ± 0.322
0.721HisThr: 0.721 ± 0.241
1.225HisVal: 1.225 ± 0.308
0.144HisTrp: 0.144 ± 0.112
0.576HisTyr: 0.576 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
4.828IleAla: 4.828 ± 0.539
0.576IleCys: 0.576 ± 0.278
4.035IleAsp: 4.035 ± 0.514
2.738IleGlu: 2.738 ± 0.389
0.937IlePhe: 0.937 ± 0.219
4.035IleGly: 4.035 ± 0.546
0.721IleHis: 0.721 ± 0.248
2.162IleIle: 2.162 ± 0.403
2.666IleLys: 2.666 ± 0.322
2.81IleLeu: 2.81 ± 0.49
0.937IleMet: 0.937 ± 0.232
2.09IleAsn: 2.09 ± 0.423
2.018IlePro: 2.018 ± 0.436
2.522IleGln: 2.522 ± 0.438
2.09IleArg: 2.09 ± 0.446
2.45IleSer: 2.45 ± 0.463
3.243IleThr: 3.243 ± 0.643
4.54IleVal: 4.54 ± 0.489
0.36IleTrp: 0.36 ± 0.2
1.874IleTyr: 1.874 ± 0.367
0.0IleXaa: 0.0 ± 0.0
Lys
5.188LysAla: 5.188 ± 0.955
0.576LysCys: 0.576 ± 0.242
1.946LysAsp: 1.946 ± 0.578
2.018LysGlu: 2.018 ± 0.552
1.153LysPhe: 1.153 ± 0.276
3.099LysGly: 3.099 ± 0.486
0.937LysHis: 0.937 ± 0.238
1.946LysIle: 1.946 ± 0.338
1.513LysLys: 1.513 ± 0.365
4.324LysLeu: 4.324 ± 0.661
0.793LysMet: 0.793 ± 0.22
1.513LysAsn: 1.513 ± 0.367
2.45LysPro: 2.45 ± 0.452
2.234LysGln: 2.234 ± 0.346
3.243LysArg: 3.243 ± 0.613
2.522LysSer: 2.522 ± 0.389
3.027LysThr: 3.027 ± 0.473
2.306LysVal: 2.306 ± 0.324
0.576LysTrp: 0.576 ± 0.248
1.225LysTyr: 1.225 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
10.089LeuAla: 10.089 ± 1.254
0.649LeuCys: 0.649 ± 0.261
4.756LeuAsp: 4.756 ± 0.488
3.459LeuGlu: 3.459 ± 0.526
2.594LeuPhe: 2.594 ± 0.366
5.261LeuGly: 5.261 ± 0.621
1.585LeuHis: 1.585 ± 0.349
3.603LeuIle: 3.603 ± 0.532
3.099LeuLys: 3.099 ± 0.443
5.188LeuLeu: 5.188 ± 0.699
2.018LeuMet: 2.018 ± 0.421
3.315LeuAsn: 3.315 ± 0.415
3.603LeuPro: 3.603 ± 0.519
4.396LeuGln: 4.396 ± 0.53
6.197LeuArg: 6.197 ± 0.808
3.963LeuSer: 3.963 ± 0.59
5.693LeuThr: 5.693 ± 0.7
5.116LeuVal: 5.116 ± 0.497
0.865LeuTrp: 0.865 ± 0.245
1.657LeuTyr: 1.657 ± 0.308
0.0LeuXaa: 0.0 ± 0.0
Met
3.459MetAla: 3.459 ± 0.503
0.072MetCys: 0.072 ± 0.066
0.865MetAsp: 0.865 ± 0.262
1.297MetGlu: 1.297 ± 0.331
0.793MetPhe: 0.793 ± 0.24
1.729MetGly: 1.729 ± 0.339
0.432MetHis: 0.432 ± 0.167
0.937MetIle: 0.937 ± 0.28
1.225MetLys: 1.225 ± 0.273
2.018MetLeu: 2.018 ± 0.385
0.649MetMet: 0.649 ± 0.2
1.297MetAsn: 1.297 ± 0.378
0.865MetPro: 0.865 ± 0.226
1.153MetGln: 1.153 ± 0.325
1.802MetArg: 1.802 ± 0.398
2.018MetSer: 2.018 ± 0.378
2.666MetThr: 2.666 ± 0.426
1.513MetVal: 1.513 ± 0.307
0.072MetTrp: 0.072 ± 0.065
0.576MetTyr: 0.576 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
5.909AsnAla: 5.909 ± 0.817
0.288AsnCys: 0.288 ± 0.152
1.081AsnAsp: 1.081 ± 0.26
0.865AsnGlu: 0.865 ± 0.27
1.153AsnPhe: 1.153 ± 0.318
5.116AsnGly: 5.116 ± 0.845
0.36AsnHis: 0.36 ± 0.155
1.369AsnIle: 1.369 ± 0.267
1.225AsnLys: 1.225 ± 0.287
2.738AsnLeu: 2.738 ± 0.457
1.009AsnMet: 1.009 ± 0.251
1.297AsnAsn: 1.297 ± 0.317
3.099AsnPro: 3.099 ± 0.686
1.946AsnGln: 1.946 ± 0.349
1.802AsnArg: 1.802 ± 0.318
2.738AsnSer: 2.738 ± 0.506
2.162AsnThr: 2.162 ± 0.608
3.171AsnVal: 3.171 ± 0.543
1.009AsnTrp: 1.009 ± 0.27
1.513AsnTyr: 1.513 ± 0.291
0.0AsnXaa: 0.0 ± 0.0
Pro
4.9ProAla: 4.9 ± 0.642
0.288ProCys: 0.288 ± 0.13
2.666ProAsp: 2.666 ± 0.501
2.81ProGlu: 2.81 ± 0.603
1.225ProPhe: 1.225 ± 0.274
2.882ProGly: 2.882 ± 0.436
0.721ProHis: 0.721 ± 0.222
2.45ProIle: 2.45 ± 0.41
1.369ProLys: 1.369 ± 0.308
2.306ProLeu: 2.306 ± 0.379
0.937ProMet: 0.937 ± 0.259
2.378ProAsn: 2.378 ± 0.568
2.162ProPro: 2.162 ± 0.494
2.018ProGln: 2.018 ± 0.384
2.162ProArg: 2.162 ± 0.443
3.099ProSer: 3.099 ± 0.46
4.396ProThr: 4.396 ± 0.558
3.531ProVal: 3.531 ± 0.556
0.432ProTrp: 0.432 ± 0.172
0.865ProTyr: 0.865 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
7.062GlnAla: 7.062 ± 0.736
0.576GlnCys: 0.576 ± 0.189
2.306GlnAsp: 2.306 ± 0.398
2.306GlnGlu: 2.306 ± 0.397
1.657GlnPhe: 1.657 ± 0.315
3.675GlnGly: 3.675 ± 0.509
1.081GlnHis: 1.081 ± 0.293
2.378GlnIle: 2.378 ± 0.499
1.729GlnLys: 1.729 ± 0.306
3.243GlnLeu: 3.243 ± 0.554
1.225GlnMet: 1.225 ± 0.263
1.585GlnAsn: 1.585 ± 0.362
2.306GlnPro: 2.306 ± 0.409
3.387GlnGln: 3.387 ± 0.625
2.594GlnArg: 2.594 ± 0.497
3.531GlnSer: 3.531 ± 0.572
3.099GlnThr: 3.099 ± 0.472
3.243GlnVal: 3.243 ± 0.512
0.649GlnTrp: 0.649 ± 0.248
0.937GlnTyr: 0.937 ± 0.237
0.0GlnXaa: 0.0 ± 0.0
Arg
6.486ArgAla: 6.486 ± 0.805
0.937ArgCys: 0.937 ± 0.309
2.955ArgAsp: 2.955 ± 0.467
4.108ArgGlu: 4.108 ± 0.652
2.234ArgPhe: 2.234 ± 0.438
3.315ArgGly: 3.315 ± 0.651
1.081ArgHis: 1.081 ± 0.282
3.387ArgIle: 3.387 ± 0.546
3.315ArgLys: 3.315 ± 0.816
4.612ArgLeu: 4.612 ± 0.591
1.802ArgMet: 1.802 ± 0.437
2.162ArgAsn: 2.162 ± 0.444
2.018ArgPro: 2.018 ± 0.382
2.45ArgGln: 2.45 ± 0.436
3.531ArgArg: 3.531 ± 0.643
2.738ArgSer: 2.738 ± 0.464
2.666ArgThr: 2.666 ± 0.531
3.747ArgVal: 3.747 ± 0.574
1.225ArgTrp: 1.225 ± 0.285
1.081ArgTyr: 1.081 ± 0.242
0.0ArgXaa: 0.0 ± 0.0
Ser
7.494SerAla: 7.494 ± 0.98
0.576SerCys: 0.576 ± 0.205
3.387SerAsp: 3.387 ± 0.589
2.234SerGlu: 2.234 ± 0.357
2.018SerPhe: 2.018 ± 0.331
7.422SerGly: 7.422 ± 1.237
0.721SerHis: 0.721 ± 0.213
4.035SerIle: 4.035 ± 0.573
2.81SerLys: 2.81 ± 0.473
4.972SerLeu: 4.972 ± 0.583
1.441SerMet: 1.441 ± 0.324
2.594SerAsn: 2.594 ± 0.546
3.027SerPro: 3.027 ± 0.402
2.882SerGln: 2.882 ± 0.44
2.594SerArg: 2.594 ± 0.528
4.468SerSer: 4.468 ± 0.801
4.252SerThr: 4.252 ± 0.717
4.18SerVal: 4.18 ± 0.594
0.865SerTrp: 0.865 ± 0.249
1.946SerTyr: 1.946 ± 0.422
0.0SerXaa: 0.0 ± 0.0
Thr
8.864ThrAla: 8.864 ± 1.036
0.649ThrCys: 0.649 ± 0.212
3.603ThrAsp: 3.603 ± 0.474
2.09ThrGlu: 2.09 ± 0.421
2.09ThrPhe: 2.09 ± 0.361
5.405ThrGly: 5.405 ± 1.036
1.153ThrHis: 1.153 ± 0.377
2.522ThrIle: 2.522 ± 0.35
2.09ThrLys: 2.09 ± 0.34
6.774ThrLeu: 6.774 ± 0.802
1.153ThrMet: 1.153 ± 0.267
3.027ThrAsn: 3.027 ± 0.597
3.603ThrPro: 3.603 ± 0.493
2.955ThrGln: 2.955 ± 0.498
3.171ThrArg: 3.171 ± 0.487
4.396ThrSer: 4.396 ± 0.741
3.963ThrThr: 3.963 ± 0.877
5.909ThrVal: 5.909 ± 0.935
1.009ThrTrp: 1.009 ± 0.282
1.946ThrTyr: 1.946 ± 0.345
0.0ThrXaa: 0.0 ± 0.0
Val
9.152ValAla: 9.152 ± 0.936
1.297ValCys: 1.297 ± 0.422
3.675ValAsp: 3.675 ± 0.601
3.819ValGlu: 3.819 ± 0.555
1.729ValPhe: 1.729 ± 0.325
5.693ValGly: 5.693 ± 0.597
1.153ValHis: 1.153 ± 0.329
3.027ValIle: 3.027 ± 0.455
2.738ValLys: 2.738 ± 0.582
4.684ValLeu: 4.684 ± 0.666
2.018ValMet: 2.018 ± 0.466
3.243ValAsn: 3.243 ± 0.481
2.882ValPro: 2.882 ± 0.459
3.747ValGln: 3.747 ± 0.652
3.603ValArg: 3.603 ± 0.503
5.261ValSer: 5.261 ± 0.748
6.413ValThr: 6.413 ± 1.017
5.044ValVal: 5.044 ± 0.897
1.513ValTrp: 1.513 ± 0.289
1.802ValTyr: 1.802 ± 0.404
0.0ValXaa: 0.0 ± 0.0
Trp
1.513TrpAla: 1.513 ± 0.383
0.216TrpCys: 0.216 ± 0.128
0.576TrpAsp: 0.576 ± 0.196
0.721TrpGlu: 0.721 ± 0.224
0.649TrpPhe: 0.649 ± 0.257
0.576TrpGly: 0.576 ± 0.199
0.432TrpHis: 0.432 ± 0.186
0.649TrpIle: 0.649 ± 0.176
0.649TrpLys: 0.649 ± 0.206
1.585TrpLeu: 1.585 ± 0.299
0.36TrpMet: 0.36 ± 0.171
0.793TrpAsn: 0.793 ± 0.228
1.081TrpPro: 1.081 ± 0.279
0.793TrpGln: 0.793 ± 0.261
0.865TrpArg: 0.865 ± 0.244
1.225TrpSer: 1.225 ± 0.301
0.793TrpThr: 0.793 ± 0.225
1.441TrpVal: 1.441 ± 0.305
0.0TrpTrp: 0.0 ± 0.0
0.649TrpTyr: 0.649 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.099TyrAla: 3.099 ± 0.49
0.649TyrCys: 0.649 ± 0.196
1.729TyrAsp: 1.729 ± 0.3
1.009TyrGlu: 1.009 ± 0.388
0.865TyrPhe: 0.865 ± 0.205
2.81TyrGly: 2.81 ± 0.58
0.432TyrHis: 0.432 ± 0.209
1.369TyrIle: 1.369 ± 0.294
1.225TyrLys: 1.225 ± 0.28
2.09TyrLeu: 2.09 ± 0.506
0.793TyrMet: 0.793 ± 0.205
0.793TyrAsn: 0.793 ± 0.255
1.009TyrPro: 1.009 ± 0.241
1.441TyrGln: 1.441 ± 0.346
2.882TyrArg: 2.882 ± 0.424
2.306TyrSer: 2.306 ± 0.414
1.513TyrThr: 1.513 ± 0.272
2.594TyrVal: 2.594 ± 0.53
0.432TyrTrp: 0.432 ± 0.205
1.585TyrTyr: 1.585 ± 0.37
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (13878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski