Campylobacter phage CJIE4-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2KLX1|X2KLX1_9CAUD Uncharacterized protein OS=Campylobacter phage CJIE4-4 OX=1470461 GN=00-6200_00021 PE=4 SV=1
MM1 pKa = 7.63PSDD4 pKa = 3.25RR5 pKa = 11.84VEE7 pKa = 4.26IEE9 pKa = 4.47LFTGFYY15 pKa = 10.35DD16 pKa = 3.65KK17 pKa = 10.88KK18 pKa = 10.83GNKK21 pKa = 9.04IYY23 pKa = 10.72EE24 pKa = 4.02GDD26 pKa = 3.19ILYY29 pKa = 10.69SFEE32 pKa = 4.18GCSEE36 pKa = 4.13DD37 pKa = 3.9EE38 pKa = 3.94AFKK41 pKa = 11.17YY42 pKa = 10.35KK43 pKa = 10.76VVFKK47 pKa = 10.74EE48 pKa = 4.07GAFYY52 pKa = 10.71LVEE55 pKa = 5.49CGDD58 pKa = 5.39DD59 pKa = 3.83GEE61 pKa = 4.8EE62 pKa = 3.96WDD64 pKa = 4.64EE65 pKa = 4.27DD66 pKa = 4.12LLSEE70 pKa = 4.6FCLEE74 pKa = 3.87EE75 pKa = 4.35LEE77 pKa = 4.43IVGNIHH83 pKa = 7.0EE84 pKa = 4.43NAEE87 pKa = 4.16LLNEE91 pKa = 4.35NKK93 pKa = 10.19PSS95 pKa = 3.37

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2KXH0|X2KXH0_9CAUD Uncharacterized protein OS=Campylobacter phage CJIE4-4 OX=1470461 GN=00-6200_00031 PE=4 SV=1
MM1 pKa = 7.63IKK3 pKa = 10.6LPMVIMLFYY12 pKa = 11.3LNLKK16 pKa = 9.21KK17 pKa = 10.77KK18 pKa = 10.8NEE20 pKa = 4.07FKK22 pKa = 10.98SKK24 pKa = 11.08NKK26 pKa = 8.36IQKK29 pKa = 9.39IKK31 pKa = 10.6RR32 pKa = 11.84KK33 pKa = 8.43EE34 pKa = 3.7LL35 pKa = 3.47

Molecular weight: 4.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11232

34

1899

193.7

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.885 ± 0.44

1.068 ± 0.24

5.057 ± 0.24

8.761 ± 0.42

5.404 ± 0.322

4.443 ± 0.353

1.042 ± 0.141

8.137 ± 0.348

11.77 ± 0.449

10.167 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.157

7.835 ± 0.339

2.039 ± 0.224

3.793 ± 0.31

2.733 ± 0.297

6.766 ± 0.506

4.558 ± 0.269

3.98 ± 0.28

0.828 ± 0.094

3.517 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski