Flavobacterium sp. 316

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6TLC6|A0A0D6TLC6_9FLAO Uncharacterized protein OS=Flavobacterium sp. 316 OX=1603293 GN=SY27_09405 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.25SLKK5 pKa = 10.28INLLKK10 pKa = 10.52KK11 pKa = 9.45IQYY14 pKa = 10.74AFIAFLYY21 pKa = 10.42CIYY24 pKa = 10.06CHH26 pKa = 6.57NYY28 pKa = 9.77EE29 pKa = 4.94ILAQNQLYY37 pKa = 7.23PTTFDD42 pKa = 4.13LSTLNGSNGFEE53 pKa = 4.05VSGLSTNLQFGAEE66 pKa = 4.15SQFIGDD72 pKa = 3.95INNDD76 pKa = 3.42GLEE79 pKa = 4.62DD80 pKa = 3.34IAFGSGTATASGLQYY95 pKa = 11.18AGAAYY100 pKa = 9.51IIFGSSVPYY109 pKa = 9.58PSPFDD114 pKa = 4.53LSTLDD119 pKa = 3.43GSNGFTVDD127 pKa = 4.12GIAYY131 pKa = 9.39DD132 pKa = 3.86EE133 pKa = 4.42RR134 pKa = 11.84RR135 pKa = 11.84GATITGPGDD144 pKa = 3.38INGDD148 pKa = 3.95GIDD151 pKa = 4.16DD152 pKa = 5.4LIIGSSSTSPDD163 pKa = 3.19EE164 pKa = 3.63IVIYY168 pKa = 8.96GTTSFPALITINDD181 pKa = 3.42INGTNGFLIDD191 pKa = 4.16TPGSNQITGLGDD203 pKa = 3.39VNGDD207 pKa = 4.17GINDD211 pKa = 4.54FIIGTPHH218 pKa = 7.15WSGQAWIIFGRR229 pKa = 11.84SSNFPTSIDD238 pKa = 3.69ASWLDD243 pKa = 3.38GTKK246 pKa = 10.63GFKK249 pKa = 9.79TSSFPGSRR257 pKa = 11.84PSYY260 pKa = 9.8KK261 pKa = 10.45VGGAGDD267 pKa = 3.7INNDD271 pKa = 3.03GFKK274 pKa = 10.72DD275 pKa = 3.43ILIGNWDD282 pKa = 3.75STNPAISYY290 pKa = 10.25LLFGKK295 pKa = 7.62GTPFSPLVDD304 pKa = 3.87LTTLDD309 pKa = 3.65GTDD312 pKa = 3.99GFSIDD317 pKa = 3.75NNGGNFLTFVGTLGDD332 pKa = 3.92INNDD336 pKa = 3.88GIDD339 pKa = 3.79DD340 pKa = 4.6FFSEE344 pKa = 4.4TNAIYY349 pKa = 10.44GSTDD353 pKa = 3.11PFPAHH358 pKa = 6.56FPLSSLNGTDD368 pKa = 4.46GFTLPGQLTSAGIGDD383 pKa = 3.91INMDD387 pKa = 4.5GIDD390 pKa = 4.61DD391 pKa = 5.25FISVYY396 pKa = 10.41GSSGRR401 pKa = 11.84AYY403 pKa = 9.47IVFGSTSGFPNPINEE418 pKa = 4.23TTLNGVNGFIIEE430 pKa = 4.35GLNPTNIARR439 pKa = 11.84PVSGNGDD446 pKa = 3.31INGDD450 pKa = 3.96GISDD454 pKa = 3.77FVVGSPYY461 pKa = 10.69TPTTQIGKK469 pKa = 9.72SYY471 pKa = 10.83VIFGGDD477 pKa = 3.53HH478 pKa = 5.87YY479 pKa = 11.92AMPLNIEE486 pKa = 4.19YY487 pKa = 8.97PQTEE491 pKa = 4.51NVTPTALTLIVNGPEE506 pKa = 3.81TGTIHH511 pKa = 6.64YY512 pKa = 10.51AIFPGNNAGTVTHH525 pKa = 6.83NDD527 pKa = 2.91ILNGTGAIANGNFLMNVQNGNVNNIVSSLPTNSSFDD563 pKa = 3.34VYY565 pKa = 11.42LFLEE569 pKa = 4.81DD570 pKa = 4.83NAGNWTQIYY579 pKa = 10.28DD580 pKa = 4.04LNNVLDD586 pKa = 4.27NEE588 pKa = 4.26DD589 pKa = 4.81FILSEE594 pKa = 3.96TDD596 pKa = 2.82IYY598 pKa = 10.8PNPTTSSINVNLTTYY613 pKa = 8.96ATYY616 pKa = 10.95EE617 pKa = 4.39LITINGQVIQKK628 pKa = 10.8GEE630 pKa = 4.4LINGKK635 pKa = 9.13NVVDD639 pKa = 4.04MSSLSKK645 pKa = 10.68GLYY648 pKa = 9.19FLYY651 pKa = 10.87VKK653 pKa = 9.73TDD655 pKa = 2.73AGTATKK661 pKa = 10.53KK662 pKa = 10.29IMKK665 pKa = 9.71KK666 pKa = 10.39

Molecular weight: 70.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6TPJ8|A0A0D6TPJ8_9FLAO Pseudouridylate synthase OS=Flavobacterium sp. 316 OX=1603293 GN=SY27_08435 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3356

0

3356

1094763

46

3465

326.2

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.482 ± 0.048

0.787 ± 0.013

5.266 ± 0.034

6.895 ± 0.046

5.493 ± 0.037

5.838 ± 0.047

1.636 ± 0.018

8.916 ± 0.043

8.55 ± 0.06

9.142 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.046 ± 0.022

7.067 ± 0.048

3.142 ± 0.025

3.318 ± 0.025

2.987 ± 0.027

6.568 ± 0.038

5.829 ± 0.045

5.737 ± 0.037

0.98 ± 0.015

4.316 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski