Amino acid dipepetide frequency for Flavobacterium sp. 316

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.029AlaAla: 3.029 ± 0.071	0.47AlaCys: 0.47 ± 0.024	2.489AlaAsp: 2.489 ± 0.053	3.329AlaGlu: 3.329 ± 0.065	2.998AlaPhe: 2.998 ± 0.052	3.312AlaGly: 3.312 ± 0.065	0.949AlaHis: 0.949 ± 0.031	5.435AlaIle: 5.435 ± 0.075	4.554AlaLys: 4.554 ± 0.083	5.41AlaLeu: 5.41 ± 0.088	1.209AlaMet: 1.209 ± 0.042	3.454AlaAsn: 3.454 ± 0.064	1.556AlaPro: 1.556 ± 0.048	1.969AlaGln: 1.969 ± 0.046	1.595AlaArg: 1.595 ± 0.048	3.729AlaSer: 3.729 ± 0.069	3.458AlaThr: 3.458 ± 0.065	3.287AlaVal: 3.287 ± 0.065	0.469AlaTrp: 0.469 ± 0.023	2.119AlaTyr: 2.119 ± 0.057	0.002AlaXaa: 0.002 ± 0.001
Cys	0.386CysAla: 0.386 ± 0.019	0.101CysCys: 0.101 ± 0.01	0.449CysAsp: 0.449 ± 0.024	0.512CysGlu: 0.512 ± 0.024	0.438CysPhe: 0.438 ± 0.021	0.589CysGly: 0.589 ± 0.025	0.184CysHis: 0.184 ± 0.015	0.638CysIle: 0.638 ± 0.024	0.56CysLys: 0.56 ± 0.025	0.66CysLeu: 0.66 ± 0.028	0.138CysMet: 0.138 ± 0.011	0.525CysAsn: 0.525 ± 0.022	0.371CysPro: 0.371 ± 0.022	0.215CysGln: 0.215 ± 0.016	0.187CysArg: 0.187 ± 0.013	0.614CysSer: 0.614 ± 0.027	0.462CysThr: 0.462 ± 0.027	0.439CysVal: 0.439 ± 0.023	0.057CysTrp: 0.057 ± 0.007	0.343CysTyr: 0.343 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.962AspAla: 2.962 ± 0.06	0.443AspCys: 0.443 ± 0.021	2.659AspAsp: 2.659 ± 0.063	3.781AspGlu: 3.781 ± 0.069	3.789AspPhe: 3.789 ± 0.064	3.182AspGly: 3.182 ± 0.075	0.685AspHis: 0.685 ± 0.024	4.617AspIle: 4.617 ± 0.07	4.513AspLys: 4.513 ± 0.092	4.916AspLeu: 4.916 ± 0.062	0.96AspMet: 0.96 ± 0.029	3.399AspAsn: 3.399 ± 0.063	1.398AspPro: 1.398 ± 0.045	1.156AspGln: 1.156 ± 0.031	1.595AspArg: 1.595 ± 0.044	3.135AspSer: 3.135 ± 0.053	2.616AspThr: 2.616 ± 0.048	3.298AspVal: 3.298 ± 0.065	0.701AspTrp: 0.701 ± 0.028	2.855AspTyr: 2.855 ± 0.05	0.004AspXaa: 0.004 ± 0.002
Glu	3.901GluAla: 3.901 ± 0.073	0.395GluCys: 0.395 ± 0.021	3.34GluAsp: 3.34 ± 0.063	5.252GluGlu: 5.252 ± 0.098	3.135GluPhe: 3.135 ± 0.052	3.429GluGly: 3.429 ± 0.069	0.989GluHis: 0.989 ± 0.034	6.71GluIle: 6.71 ± 0.087	7.208GluLys: 7.208 ± 0.11	6.254GluLeu: 6.254 ± 0.093	1.674GluMet: 1.674 ± 0.046	5.812GluAsn: 5.812 ± 0.085	1.484GluPro: 1.484 ± 0.039	2.264GluGln: 2.264 ± 0.046	2.268GluArg: 2.268 ± 0.056	3.454GluSer: 3.454 ± 0.055	3.899GluThr: 3.899 ± 0.063	4.141GluVal: 4.141 ± 0.077	0.641GluTrp: 0.641 ± 0.023	2.696GluTyr: 2.696 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.706PheAla: 2.706 ± 0.056	0.495PheCys: 0.495 ± 0.025	3.298PheAsp: 3.298 ± 0.065	3.815PheGlu: 3.815 ± 0.062	3.063PhePhe: 3.063 ± 0.078	3.42PheGly: 3.42 ± 0.07	0.867PheHis: 0.867 ± 0.029	4.635PheIle: 4.635 ± 0.078	4.036PheLys: 4.036 ± 0.069	5.006PheLeu: 5.006 ± 0.087	1.11PheMet: 1.11 ± 0.028	3.767PheAsn: 3.767 ± 0.073	1.824PhePro: 1.824 ± 0.039	1.746PheGln: 1.746 ± 0.041	1.508PheArg: 1.508 ± 0.038	4.377PheSer: 4.377 ± 0.067	3.14PheThr: 3.14 ± 0.054	3.005PheVal: 3.005 ± 0.058	0.586PheTrp: 0.586 ± 0.026	2.518PheTyr: 2.518 ± 0.05	0.003PheXaa: 0.003 ± 0.002
Gly	3.427GlyAla: 3.427 ± 0.071	0.578GlyCys: 0.578 ± 0.028	2.798GlyAsp: 2.798 ± 0.063	3.234GlyGlu: 3.234 ± 0.068	3.456GlyPhe: 3.456 ± 0.063	3.937GlyGly: 3.937 ± 0.089	0.977GlyHis: 0.977 ± 0.036	5.37GlyIle: 5.37 ± 0.078	4.987GlyLys: 4.987 ± 0.08	5.19GlyLeu: 5.19 ± 0.076	1.332GlyMet: 1.332 ± 0.037	3.965GlyAsn: 3.965 ± 0.08	1.096GlyPro: 1.096 ± 0.034	1.693GlyGln: 1.693 ± 0.041	1.72GlyArg: 1.72 ± 0.045	3.688GlySer: 3.688 ± 0.073	3.938GlyThr: 3.938 ± 0.093	3.623GlyVal: 3.623 ± 0.062	0.637GlyTrp: 0.637 ± 0.024	2.736GlyTyr: 2.736 ± 0.057	0.002GlyXaa: 0.002 ± 0.001
His	0.797HisAla: 0.797 ± 0.028	0.175HisCys: 0.175 ± 0.014	0.795HisAsp: 0.795 ± 0.026	0.977HisGlu: 0.977 ± 0.032	1.223HisPhe: 1.223 ± 0.037	0.874HisGly: 0.874 ± 0.026	0.395HisHis: 0.395 ± 0.02	1.345HisIle: 1.345 ± 0.04	1.191HisLys: 1.191 ± 0.037	1.667HisLeu: 1.667 ± 0.045	0.256HisMet: 0.256 ± 0.015	1.028HisAsn: 1.028 ± 0.027	0.747HisPro: 0.747 ± 0.025	0.656HisGln: 0.656 ± 0.027	0.538HisArg: 0.538 ± 0.021	1.069HisSer: 1.069 ± 0.033	0.823HisThr: 0.823 ± 0.027	0.778HisVal: 0.778 ± 0.026	0.184HisTrp: 0.184 ± 0.015	0.847HisTyr: 0.847 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.461IleAla: 5.461 ± 0.077	0.716IleCys: 0.716 ± 0.028	5.405IleAsp: 5.405 ± 0.065	6.747IleGlu: 6.747 ± 0.088	4.037IlePhe: 4.037 ± 0.072	5.267IleGly: 5.267 ± 0.086	1.45IleHis: 1.45 ± 0.038	7.793IleIle: 7.793 ± 0.109	7.17IleLys: 7.17 ± 0.093	7.85IleLeu: 7.85 ± 0.106	1.548IleMet: 1.548 ± 0.039	5.917IleAsn: 5.917 ± 0.089	3.336IlePro: 3.336 ± 0.058	3.044IleGln: 3.044 ± 0.055	2.483IleArg: 2.483 ± 0.047	6.335IleSer: 6.335 ± 0.075	5.409IleThr: 5.409 ± 0.078	5.133IleVal: 5.133 ± 0.07	0.726IleTrp: 0.726 ± 0.026	3.336IleTyr: 3.336 ± 0.068	0.002IleXaa: 0.002 ± 0.002
Lys	4.773LysAla: 4.773 ± 0.074	0.439LysCys: 0.439 ± 0.021	4.518LysAsp: 4.518 ± 0.072	7.415LysGlu: 7.415 ± 0.106	3.318LysPhe: 3.318 ± 0.063	4.505LysGly: 4.505 ± 0.074	1.368LysHis: 1.368 ± 0.036	8.061LysIle: 8.061 ± 0.091	8.106LysLys: 8.106 ± 0.128	6.971LysLeu: 6.971 ± 0.099	2.245LysMet: 2.245 ± 0.045	6.496LysAsn: 6.496 ± 0.096	2.426LysPro: 2.426 ± 0.057	2.867LysGln: 2.867 ± 0.063	2.705LysArg: 2.705 ± 0.058	4.908LysSer: 4.908 ± 0.076	5.155LysThr: 5.155 ± 0.073	4.792LysVal: 4.792 ± 0.078	0.807LysTrp: 0.807 ± 0.028	3.621LysTyr: 3.621 ± 0.064	0.004LysXaa: 0.004 ± 0.002
Leu	5.127LeuAla: 5.127 ± 0.078	0.625LeuCys: 0.625 ± 0.025	4.915LeuAsp: 4.915 ± 0.069	6.326LeuGlu: 6.326 ± 0.085	5.406LeuPhe: 5.406 ± 0.092	5.37LeuGly: 5.37 ± 0.086	1.513LeuHis: 1.513 ± 0.038	7.547LeuIle: 7.547 ± 0.104	8.039LeuLys: 8.039 ± 0.101	8.732LeuLeu: 8.732 ± 0.104	1.848LeuMet: 1.848 ± 0.045	6.307LeuAsn: 6.307 ± 0.086	3.319LeuPro: 3.319 ± 0.053	3.23LeuGln: 3.23 ± 0.06	2.708LeuArg: 2.708 ± 0.05	6.542LeuSer: 6.542 ± 0.082	4.902LeuThr: 4.902 ± 0.069	4.818LeuVal: 4.818 ± 0.07	0.778LeuTrp: 0.778 ± 0.028	3.362LeuTyr: 3.362 ± 0.054	0.003LeuXaa: 0.003 ± 0.002
Met	1.299MetAla: 1.299 ± 0.037	0.143MetCys: 0.143 ± 0.012	1.016MetAsp: 1.016 ± 0.028	1.295MetGlu: 1.295 ± 0.038	0.814MetPhe: 0.814 ± 0.028	1.208MetGly: 1.208 ± 0.038	0.35MetHis: 0.35 ± 0.019	1.611MetIle: 1.611 ± 0.04	2.273MetLys: 2.273 ± 0.043	1.815MetLeu: 1.815 ± 0.043	0.54MetMet: 0.54 ± 0.024	1.391MetAsn: 1.391 ± 0.036	0.693MetPro: 0.693 ± 0.027	0.786MetGln: 0.786 ± 0.026	0.803MetArg: 0.803 ± 0.029	1.385MetSer: 1.385 ± 0.033	1.075MetThr: 1.075 ± 0.028	1.055MetVal: 1.055 ± 0.031	0.153MetTrp: 0.153 ± 0.012	0.75MetTyr: 0.75 ± 0.027	0.001MetXaa: 0.001 ± 0.001
Asn	3.779AsnAla: 3.779 ± 0.068	0.597AsnCys: 0.597 ± 0.025	3.778AsnAsp: 3.778 ± 0.064	4.863AsnGlu: 4.863 ± 0.069	3.671AsnPhe: 3.671 ± 0.064	4.35AsnGly: 4.35 ± 0.092	1.231AsnHis: 1.231 ± 0.041	5.847AsnIle: 5.847 ± 0.083	5.617AsnLys: 5.617 ± 0.085	6.031AsnLeu: 6.031 ± 0.086	1.212AsnMet: 1.212 ± 0.032	5.09AsnAsn: 5.09 ± 0.093	2.945AsnPro: 2.945 ± 0.058	2.709AsnGln: 2.709 ± 0.047	2.099AsnArg: 2.099 ± 0.048	4.498AsnSer: 4.498 ± 0.079	4.171AsnThr: 4.171 ± 0.071	3.749AsnVal: 3.749 ± 0.065	0.906AsnTrp: 0.906 ± 0.031	3.525AsnTyr: 3.525 ± 0.06	0.002AsnXaa: 0.002 ± 0.001
Pro	1.511ProAla: 1.511 ± 0.039	0.218ProCys: 0.218 ± 0.015	1.75ProAsp: 1.75 ± 0.044	2.441ProGlu: 2.441 ± 0.049	1.898ProPhe: 1.898 ± 0.045	1.444ProGly: 1.444 ± 0.042	0.505ProHis: 0.505 ± 0.022	2.917ProIle: 2.917 ± 0.057	2.625ProLys: 2.625 ± 0.052	2.692ProLeu: 2.692 ± 0.055	0.62ProMet: 0.62 ± 0.022	2.552ProAsn: 2.552 ± 0.061	0.71ProPro: 0.71 ± 0.029	0.983ProGln: 0.983 ± 0.029	0.776ProArg: 0.776 ± 0.03	2.132ProSer: 2.132 ± 0.046	1.942ProThr: 1.942 ± 0.046	2.074ProVal: 2.074 ± 0.045	0.253ProTrp: 0.253 ± 0.015	1.375ProTyr: 1.375 ± 0.034	0.001ProXaa: 0.001 ± 0.001
Gln	1.672GlnAla: 1.672 ± 0.041	0.202GlnCys: 0.202 ± 0.014	1.548GlnAsp: 1.548 ± 0.038	2.347GlnGlu: 2.347 ± 0.053	1.82GlnPhe: 1.82 ± 0.046	1.578GlnGly: 1.578 ± 0.039	0.53GlnHis: 0.53 ± 0.024	2.959GlnIle: 2.959 ± 0.054	3.177GlnLys: 3.177 ± 0.063	3.36GlnLeu: 3.36 ± 0.058	0.765GlnMet: 0.765 ± 0.026	2.625GlnAsn: 2.625 ± 0.047	0.978GlnPro: 0.978 ± 0.026	1.193GlnGln: 1.193 ± 0.037	1.002GlnArg: 1.002 ± 0.031	1.938GlnSer: 1.938 ± 0.045	1.849GlnThr: 1.849 ± 0.042	1.81GlnVal: 1.81 ± 0.044	0.318GlnTrp: 0.318 ± 0.018	1.505GlnTyr: 1.505 ± 0.04	0.003GlnXaa: 0.003 ± 0.002
Arg	1.636ArgAla: 1.636 ± 0.044	0.171ArgCys: 0.171 ± 0.011	1.468ArgAsp: 1.468 ± 0.038	2.067ArgGlu: 2.067 ± 0.046	1.736ArgPhe: 1.736 ± 0.047	1.629ArgGly: 1.629 ± 0.037	0.47ArgHis: 0.47 ± 0.018	2.796ArgIle: 2.796 ± 0.058	2.733ArgLys: 2.733 ± 0.055	2.849ArgLeu: 2.849 ± 0.056	0.808ArgMet: 0.808 ± 0.031	2.084ArgAsn: 2.084 ± 0.041	0.837ArgPro: 0.837 ± 0.03	0.897ArgGln: 0.897 ± 0.025	1.048ArgArg: 1.048 ± 0.036	1.567ArgSer: 1.567 ± 0.039	1.606ArgThr: 1.606 ± 0.037	1.809ArgVal: 1.809 ± 0.043	0.327ArgTrp: 0.327 ± 0.019	1.332ArgTyr: 1.332 ± 0.035	0.001ArgXaa: 0.001 ± 0.001
Ser	3.139SerAla: 3.139 ± 0.056	0.689SerCys: 0.689 ± 0.029	3.299SerAsp: 3.299 ± 0.066	4.021SerGlu: 4.021 ± 0.063	4.174SerPhe: 4.174 ± 0.066	4.227SerGly: 4.227 ± 0.086	1.064SerHis: 1.064 ± 0.033	6.044SerIle: 6.044 ± 0.083	5.531SerLys: 5.531 ± 0.09	6.027SerLeu: 6.027 ± 0.073	1.163SerMet: 1.163 ± 0.031	4.624SerAsn: 4.624 ± 0.082	1.958SerPro: 1.958 ± 0.046	2.28SerGln: 2.28 ± 0.045	1.905SerArg: 1.905 ± 0.042	4.62SerSer: 4.62 ± 0.091	3.627SerThr: 3.627 ± 0.066	3.726SerVal: 3.726 ± 0.061	0.649SerTrp: 0.649 ± 0.025	2.913SerTyr: 2.913 ± 0.054	0.002SerXaa: 0.002 ± 0.001
Thr	3.134ThrAla: 3.134 ± 0.064	0.42ThrCys: 0.42 ± 0.02	2.972ThrAsp: 2.972 ± 0.055	3.53ThrGlu: 3.53 ± 0.068	3.277ThrPhe: 3.277 ± 0.066	3.666ThrGly: 3.666 ± 0.068	0.973ThrHis: 0.973 ± 0.031	5.809ThrIle: 5.809 ± 0.089	4.388ThrLys: 4.388 ± 0.066	5.419ThrLeu: 5.419 ± 0.073	0.897ThrMet: 0.897 ± 0.028	4.042ThrAsn: 4.042 ± 0.075	2.271ThrPro: 2.271 ± 0.054	1.855ThrGln: 1.855 ± 0.039	1.483ThrArg: 1.483 ± 0.037	3.999ThrSer: 3.999 ± 0.08	3.822ThrThr: 3.822 ± 0.09	3.396ThrVal: 3.396 ± 0.071	0.524ThrTrp: 0.524 ± 0.024	2.408ThrTyr: 2.408 ± 0.062	0.004ThrXaa: 0.004 ± 0.002
Val	3.427ValAla: 3.427 ± 0.06	0.49ValCys: 0.49 ± 0.022	3.203ValAsp: 3.203 ± 0.059	3.81ValGlu: 3.81 ± 0.069	3.354ValPhe: 3.354 ± 0.051	3.288ValGly: 3.288 ± 0.064	0.849ValHis: 0.849 ± 0.03	4.962ValIle: 4.962 ± 0.073	4.47ValLys: 4.47 ± 0.065	5.616ValLeu: 5.616 ± 0.072	1.154ValMet: 1.154 ± 0.04	3.606ValAsn: 3.606 ± 0.057	1.851ValPro: 1.851 ± 0.04	1.632ValGln: 1.632 ± 0.043	1.709ValArg: 1.709 ± 0.041	4.116ValSer: 4.116 ± 0.063	3.389ValThr: 3.389 ± 0.068	3.666ValVal: 3.666 ± 0.064	0.506ValTrp: 0.506 ± 0.023	2.274ValTyr: 2.274 ± 0.05	0.001ValXaa: 0.001 ± 0.001
Trp	0.508TrpAla: 0.508 ± 0.021	0.108TrpCys: 0.108 ± 0.01	0.524TrpAsp: 0.524 ± 0.025	0.606TrpGlu: 0.606 ± 0.025	0.543TrpPhe: 0.543 ± 0.023	0.585TrpGly: 0.585 ± 0.027	0.211TrpHis: 0.211 ± 0.017	0.816TrpIle: 0.816 ± 0.025	0.853TrpLys: 0.853 ± 0.027	0.919TrpLeu: 0.919 ± 0.036	0.279TrpMet: 0.279 ± 0.017	0.78TrpAsn: 0.78 ± 0.031	0.161TrpPro: 0.161 ± 0.012	0.41TrpGln: 0.41 ± 0.021	0.317TrpArg: 0.317 ± 0.017	0.558TrpSer: 0.558 ± 0.024	0.518TrpThr: 0.518 ± 0.024	0.531TrpVal: 0.531 ± 0.023	0.125TrpTrp: 0.125 ± 0.011	0.454TrpTyr: 0.454 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.147TyrAla: 2.147 ± 0.048	0.39TyrCys: 0.39 ± 0.02	2.436TyrAsp: 2.436 ± 0.055	2.58TyrGlu: 2.58 ± 0.045	2.77TyrPhe: 2.77 ± 0.056	2.52TyrGly: 2.52 ± 0.052	0.803TyrHis: 0.803 ± 0.028	3.254TyrIle: 3.254 ± 0.065	3.468TyrLys: 3.468 ± 0.057	4.028TyrLeu: 4.028 ± 0.07	0.697TyrMet: 0.697 ± 0.028	3.201TyrAsn: 3.201 ± 0.071	1.457TyrPro: 1.457 ± 0.037	1.592TyrGln: 1.592 ± 0.038	1.42TyrArg: 1.42 ± 0.039	3.018TyrSer: 3.018 ± 0.062	2.486TyrThr: 2.486 ± 0.057	2.243TyrVal: 2.243 ± 0.044	0.458TyrTrp: 0.458 ± 0.019	2.194TyrTyr: 2.194 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.003XaaAsp: 0.003 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.004XaaPhe: 0.004 ± 0.003	0.003XaaGly: 0.003 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.003XaaLys: 0.003 ± 0.002	0.005XaaLeu: 0.005 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.004XaaAsn: 0.004 ± 0.002	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.003

Statistics based on 3356 proteins (1094763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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