Lettuce yellow mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Lettuce yellow mottle cytorhabdovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1NNZ2|B1NNZ2_9RHAB Protein 3 OS=Lettuce yellow mottle virus OX=471285 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.9NEE4 pKa = 4.14NLDD7 pKa = 3.95FEE9 pKa = 5.21SVDD12 pKa = 3.45TSLLRR17 pKa = 11.84SPNNFAANNDD27 pKa = 3.88GTSSPDD33 pKa = 3.19VMFDD37 pKa = 3.3DD38 pKa = 4.0TTIPDD43 pKa = 3.65GVLVDD48 pKa = 3.79NSHH51 pKa = 6.48NKK53 pKa = 9.99SNNKK57 pKa = 9.54ANSEE61 pKa = 4.42TVVQLLEE68 pKa = 4.11LAAATQGVMVSDD80 pKa = 3.62IMTNTAIALAYY91 pKa = 9.9KK92 pKa = 10.51LGLDD96 pKa = 3.88ANSMEE101 pKa = 4.17WFIAGVTYY109 pKa = 11.01ANNNMLMEE117 pKa = 4.48KK118 pKa = 9.38MVSAVKK124 pKa = 10.36DD125 pKa = 3.48LQVEE129 pKa = 4.16VRR131 pKa = 11.84NIQVASNSVKK141 pKa = 10.59NSSDD145 pKa = 3.17ALASTMKK152 pKa = 10.48SNKK155 pKa = 9.08HH156 pKa = 6.51DD157 pKa = 3.46IVKK160 pKa = 10.3EE161 pKa = 3.74LDD163 pKa = 3.14KK164 pKa = 11.45TRR166 pKa = 11.84DD167 pKa = 3.48SVLNALNSLAKK178 pKa = 9.26KK179 pKa = 8.53TEE181 pKa = 4.31EE182 pKa = 4.14VQSPTEE188 pKa = 3.87VVTIGIAQGKK198 pKa = 8.94KK199 pKa = 9.93ALKK202 pKa = 9.69QLSEE206 pKa = 4.33SAIPPPQDD214 pKa = 3.05INSEE218 pKa = 4.38LLTPTLTKK226 pKa = 10.43VVSTKK231 pKa = 10.59SPAEE235 pKa = 4.13TVHH238 pKa = 6.79HH239 pKa = 6.16EE240 pKa = 4.15KK241 pKa = 10.67EE242 pKa = 4.26KK243 pKa = 11.22LMLDD247 pKa = 3.66LGFEE251 pKa = 4.1VDD253 pKa = 4.81EE254 pKa = 4.8VAGCDD259 pKa = 3.84PLVLDD264 pKa = 5.59MIITDD269 pKa = 5.37DD270 pKa = 3.94MLQVAAGGLTEE281 pKa = 4.71EE282 pKa = 4.76IKK284 pKa = 10.78EE285 pKa = 4.08ALQDD289 pKa = 3.65QAIDD293 pKa = 3.58NQMTIAQIVDD303 pKa = 2.87IMKK306 pKa = 10.49

Molecular weight: 33.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1NNZ3|B1NNZ3_9RHAB M protein OS=Lettuce yellow mottle virus OX=471285 PE=4 SV=1
MM1 pKa = 7.45LNINSVRR8 pKa = 11.84KK9 pKa = 9.13HH10 pKa = 4.43VLKK13 pKa = 10.92SGSLTSAVGTGTVYY27 pKa = 10.86SGMLNKK33 pKa = 9.74YY34 pKa = 9.3AKK36 pKa = 9.97KK37 pKa = 9.94RR38 pKa = 11.84EE39 pKa = 4.02LNIVVTSSGSNNVLIRR55 pKa = 11.84QVPLFDD61 pKa = 5.37QMDD64 pKa = 4.61LDD66 pKa = 3.83MMKK69 pKa = 10.27KK70 pKa = 10.21DD71 pKa = 3.9KK72 pKa = 11.08EE73 pKa = 4.21NNKK76 pKa = 9.57YY77 pKa = 9.9IHH79 pKa = 6.85IGCVTISIEE88 pKa = 4.02PLLHH92 pKa = 5.5QRR94 pKa = 11.84YY95 pKa = 8.8ISKK98 pKa = 10.51FGTAISGHH106 pKa = 6.35CALIDD111 pKa = 3.52STFRR115 pKa = 11.84NLDD118 pKa = 3.21EE119 pKa = 4.96SVISVHH125 pKa = 7.03KK126 pKa = 10.35YY127 pKa = 10.29CLDD130 pKa = 3.48KK131 pKa = 11.33GRR133 pKa = 11.84SDD135 pKa = 3.58YY136 pKa = 11.32VSYY139 pKa = 10.76PNHH142 pKa = 6.66CLSLSDD148 pKa = 3.22PHH150 pKa = 6.75IQKK153 pKa = 10.31RR154 pKa = 11.84LSVILGIRR162 pKa = 11.84GVNVDD167 pKa = 3.85PGVEE171 pKa = 4.05LFSVCIGYY179 pKa = 9.33IVSAVNTLHH188 pKa = 6.42PNGMKK193 pKa = 10.67GMVNFPILGTEE204 pKa = 4.17DD205 pKa = 4.15ASVDD209 pKa = 3.78DD210 pKa = 5.69LEE212 pKa = 6.32GSDD215 pKa = 3.31ISEE218 pKa = 4.38IEE220 pKa = 4.04SNYY223 pKa = 10.35NNTSIISVPSEE234 pKa = 3.5DD235 pKa = 3.42DD236 pKa = 2.92QYY238 pKa = 11.31YY239 pKa = 10.21KK240 pKa = 10.9SKK242 pKa = 10.78GGLWRR247 pKa = 11.84GITGGGRR254 pKa = 11.84VIKK257 pKa = 10.08RR258 pKa = 11.84RR259 pKa = 11.84TMRR262 pKa = 11.84TEE264 pKa = 4.0NKK266 pKa = 9.57QSAPSLLARR275 pKa = 11.84STSSRR280 pKa = 11.84IDD282 pKa = 3.34EE283 pKa = 4.12KK284 pKa = 11.09RR285 pKa = 11.84VLGVLKK291 pKa = 10.58NRR293 pKa = 11.84EE294 pKa = 3.9ITEE297 pKa = 3.84KK298 pKa = 9.56LTNIGRR304 pKa = 11.84SGRR307 pKa = 11.84FSMSS311 pKa = 2.63

Molecular weight: 34.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3860

175

2068

643.3

72.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.337 ± 1.25

1.891 ± 0.333

6.036 ± 0.297

5.829 ± 0.209

3.679 ± 0.645

5.829 ± 0.411

2.202 ± 0.292

6.917 ± 0.328

6.528 ± 0.357

9.482 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.161 ± 0.396

3.964 ± 0.638

4.352 ± 0.255

3.342 ± 0.352

5.311 ± 0.582

8.316 ± 0.447

6.554 ± 0.431

6.451 ± 0.577

1.71 ± 0.357

3.109 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski