Ustilaginoidea virens RNA virus 5
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2UQ51|A0A0S2UQ51_9VIRU RNA-directed RNA polymerase OS=Ustilaginoidea virens RNA virus 5 OX=1756615 GN=RdRp PE=3 SV=1
MM1 pKa = 7.71 ANTFNALAGVLGRR14 pKa = 11.84 PRR16 pKa = 11.84 TGTISGPAKK25 pKa = 8.38 YY26 pKa = 9.52 RR27 pKa = 11.84 KK28 pKa = 8.91 YY29 pKa = 9.72 AASMTTSVQIRR40 pKa = 11.84 GVDD43 pKa = 3.5 DD44 pKa = 5.02 LSVKK48 pKa = 10.4 RR49 pKa = 11.84 ILYY52 pKa = 9.37 EE53 pKa = 3.35 VGLRR57 pKa = 11.84 HH58 pKa = 6.98 KK59 pKa = 10.95 SRR61 pKa = 11.84 DD62 pKa = 3.37 VALAATPEE70 pKa = 4.36 TVTMIEE76 pKa = 4.0 SAYY79 pKa = 10.45 DD80 pKa = 3.48 SSGLVSDD87 pKa = 4.44 VLSGLARR94 pKa = 11.84 KK95 pKa = 9.36 FSNFSGTFDD104 pKa = 3.4 VSNLAGVVEE113 pKa = 4.7 RR114 pKa = 11.84 LAKK117 pKa = 10.47 GLAADD122 pKa = 3.57 SAYY125 pKa = 10.46 EE126 pKa = 4.32 GGCTAQGLLGGNEE139 pKa = 3.97 VRR141 pKa = 11.84 VHH143 pKa = 6.91 ALGTYY148 pKa = 7.04 TGPVGAHH155 pKa = 6.99 RR156 pKa = 11.84 DD157 pKa = 3.56 TVFIPRR163 pKa = 11.84 LVDD166 pKa = 3.67 SVLAPDD172 pKa = 3.9 VFSVMVHH179 pKa = 5.81 AVCGEE184 pKa = 4.01 GGIVATDD191 pKa = 3.81 LVEE194 pKa = 5.32 LDD196 pKa = 3.44 ANTRR200 pKa = 11.84 EE201 pKa = 3.98 PLIRR205 pKa = 11.84 DD206 pKa = 3.21 VDD208 pKa = 4.11 DD209 pKa = 4.52 GAFSTAVVDD218 pKa = 4.37 ALRR221 pKa = 11.84 LLGANFAASGQGEE234 pKa = 4.92 LFSLAVVRR242 pKa = 11.84 GLNAVLTVVGHH253 pKa = 5.2 TDD255 pKa = 2.76 EE256 pKa = 5.29 GGVVRR261 pKa = 11.84 DD262 pKa = 3.91 VLRR265 pKa = 11.84 KK266 pKa = 9.36 GAFSTPYY273 pKa = 10.42 GAVHH277 pKa = 7.04 CGLQDD282 pKa = 3.82 YY283 pKa = 9.84 PGLPALASSSTSTIAGYY300 pKa = 10.42 CDD302 pKa = 3.72 SLLLSAAALVAHH314 pKa = 7.24 CDD316 pKa = 3.23 PGVVYY321 pKa = 10.8 NGSWYY326 pKa = 8.02 PTVLQGTSPVDD337 pKa = 3.28 RR338 pKa = 11.84 EE339 pKa = 4.16 VRR341 pKa = 11.84 PGAEE345 pKa = 4.09 TPGTAEE351 pKa = 3.6 MARR354 pKa = 11.84 RR355 pKa = 11.84 NKK357 pKa = 9.98 RR358 pKa = 11.84 ALLGRR363 pKa = 11.84 FDD365 pKa = 3.74 VFSRR369 pKa = 11.84 QYY371 pKa = 9.25 TAALAKK377 pKa = 10.73 LFGLEE382 pKa = 4.1 AGGTAASRR390 pKa = 11.84 HH391 pKa = 4.61 LTASASLLDD400 pKa = 4.57 DD401 pKa = 4.89 DD402 pKa = 5.42 NRR404 pKa = 11.84 HH405 pKa = 5.87 LKK407 pKa = 9.42 FASVSPYY414 pKa = 9.84 FWVEE418 pKa = 3.52 PTSIIAHH425 pKa = 6.15 NFTGYY430 pKa = 9.62 VAEE433 pKa = 4.58 AEE435 pKa = 4.66 GFASLCGRR443 pKa = 11.84 GEE445 pKa = 4.29 RR446 pKa = 11.84 VTRR449 pKa = 11.84 PAWEE453 pKa = 4.26 QIAPAPGGDD462 pKa = 3.62 VASSSYY468 pKa = 10.54 YY469 pKa = 9.69 IRR471 pKa = 11.84 FRR473 pKa = 11.84 GARR476 pKa = 11.84 ACGYY480 pKa = 8.48 LHH482 pKa = 7.0 HH483 pKa = 6.62 FHH485 pKa = 6.61 GHH487 pKa = 6.35 ANDD490 pKa = 3.21 GMAFVVPRR498 pKa = 11.84 QLDD501 pKa = 3.61 PAGVIHH507 pKa = 6.96 PGPDD511 pKa = 3.05 QEE513 pKa = 4.57 EE514 pKa = 4.31 EE515 pKa = 3.95 VRR517 pKa = 11.84 DD518 pKa = 3.85 RR519 pKa = 11.84 LEE521 pKa = 4.16 KK522 pKa = 9.85 GAHH525 pKa = 5.65 IGRR528 pKa = 11.84 YY529 pKa = 6.79 LWRR532 pKa = 11.84 RR533 pKa = 11.84 GQSPICAPGEE543 pKa = 3.81 FLNLGEE549 pKa = 4.17 TMALRR554 pKa = 11.84 FRR556 pKa = 11.84 HH557 pKa = 5.93 LLHH560 pKa = 7.5 DD561 pKa = 3.94 EE562 pKa = 4.41 DD563 pKa = 5.89 GYY565 pKa = 11.86 AVAVHH570 pKa = 6.28 CPGHH574 pKa = 6.42 GEE576 pKa = 4.09 VEE578 pKa = 4.11 ILEE581 pKa = 4.18 VSYY584 pKa = 10.8 AASAPIGISEE594 pKa = 4.75 GPLTSAPAEE603 pKa = 3.89 ARR605 pKa = 11.84 RR606 pKa = 11.84 ARR608 pKa = 11.84 TAATRR613 pKa = 11.84 ALVSACSQAQAYY625 pKa = 8.77 SQATVDD631 pKa = 4.91 DD632 pKa = 4.73 LPLLASAPPVLRR644 pKa = 11.84 RR645 pKa = 11.84 AAQTPAKK652 pKa = 9.23 PSEE655 pKa = 4.06 GVILPGGGGKK665 pKa = 10.05 LVDD668 pKa = 4.09 VPSAPDD674 pKa = 3.6 DD675 pKa = 3.85 NEE677 pKa = 4.29 AGYY680 pKa = 8.32 TRR682 pKa = 11.84 GTAQRR687 pKa = 11.84 VVLHH691 pKa = 6.8 ADD693 pKa = 3.49 AVRR696 pKa = 11.84 GPAVEE701 pKa = 3.95 RR702 pKa = 11.84 GQRR705 pKa = 11.84 QGGGGVGPDD714 pKa = 3.58 TPATVPRR721 pKa = 11.84 PTRR724 pKa = 11.84 VVVDD728 pKa = 4.18 DD729 pKa = 5.21 GEE731 pKa = 4.47 DD732 pKa = 3.03 AGAPGRR738 pKa = 11.84 PALTAEE744 pKa = 4.49 GATGRR749 pKa = 11.84 RR750 pKa = 11.84 EE751 pKa = 3.98 PGRR754 pKa = 11.84 AEE756 pKa = 3.74
Molecular weight: 79.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.782
IPC2_protein 5.703
IPC_protein 5.766
Toseland 6.186
ProMoST 6.059
Dawson 6.033
Bjellqvist 6.046
Wikipedia 6.046
Rodwell 6.02
Grimsley 6.351
Solomon 6.033
Lehninger 6.033
Nozaki 6.313
DTASelect 6.504
Thurlkill 6.517
EMBOSS 6.478
Sillero 6.402
Patrickios 4.164
IPC_peptide 6.059
IPC2_peptide 6.427
IPC2.peptide.svr19 6.319
Protein with the highest isoelectric point:
>tr|A0A0S2UQ51|A0A0S2UQ51_9VIRU RNA-directed RNA polymerase OS=Ustilaginoidea virens RNA virus 5 OX=1756615 GN=RdRp PE=3 SV=1
MM1 pKa = 7.63 AAHH4 pKa = 6.86 LSISEE9 pKa = 3.79 RR10 pKa = 11.84 RR11 pKa = 11.84 AALGHH16 pKa = 6.73 LGDD19 pKa = 3.93 VFYY22 pKa = 11.27 GMLEE26 pKa = 3.97 KK27 pKa = 11.1 AEE29 pKa = 4.35 FPAEE33 pKa = 3.96 KK34 pKa = 9.96 FRR36 pKa = 11.84 EE37 pKa = 4.12 LRR39 pKa = 11.84 ATEE42 pKa = 3.92 QLLLLSVGGGVLNSSLALAVEE63 pKa = 4.64 FNRR66 pKa = 11.84 RR67 pKa = 11.84 VRR69 pKa = 11.84 SRR71 pKa = 11.84 VGLKK75 pKa = 9.51 GQPGLKK81 pKa = 9.16 TGVVVDD87 pKa = 3.68 PLLRR91 pKa = 11.84 VAASMLCARR100 pKa = 11.84 FAVQVEE106 pKa = 4.45 MTDD109 pKa = 3.33 SNVRR113 pKa = 11.84 RR114 pKa = 11.84 LVRR117 pKa = 11.84 LAFPEE122 pKa = 4.27 ALPPLWSGVPAEE134 pKa = 4.26 VRR136 pKa = 11.84 GKK138 pKa = 10.83 VSLSALAARR147 pKa = 11.84 ADD149 pKa = 3.58 LRR151 pKa = 11.84 EE152 pKa = 3.67 VCFPYY157 pKa = 9.29 KK158 pKa = 9.85 TVPQATRR165 pKa = 11.84 KK166 pKa = 10.34 ANVHH170 pKa = 5.96 LASLLTPKK178 pKa = 10.38 ARR180 pKa = 11.84 EE181 pKa = 3.91 LVGGLDD187 pKa = 4.79 RR188 pKa = 11.84 LIGWLAGRR196 pKa = 11.84 CSDD199 pKa = 5.04 DD200 pKa = 3.6 QVCSAIIYY208 pKa = 9.29 AHH210 pKa = 6.82 ALGSRR215 pKa = 11.84 WGPGAAEE222 pKa = 3.54 IAARR226 pKa = 11.84 YY227 pKa = 9.39 ILDD230 pKa = 3.63 PEE232 pKa = 4.64 GAVSVGLVLKK242 pKa = 11.12 AMGANSGPLGAALVEE257 pKa = 4.61 GKK259 pKa = 9.85 SLQGRR264 pKa = 11.84 GVGSLDD270 pKa = 3.33 LAKK273 pKa = 10.33 EE274 pKa = 4.07 AEE276 pKa = 4.36 QRR278 pKa = 11.84 CDD280 pKa = 3.85 PDD282 pKa = 3.31 WVAGKK287 pKa = 9.9 VLHH290 pKa = 7.02 CDD292 pKa = 3.5 PEE294 pKa = 4.11 EE295 pKa = 3.9 LRR297 pKa = 11.84 LVIRR301 pKa = 11.84 QILSEE306 pKa = 4.05 EE307 pKa = 4.04 LKK309 pKa = 10.74 GRR311 pKa = 11.84 EE312 pKa = 4.01 IVFDD316 pKa = 3.63 TPEE319 pKa = 4.06 QFWEE323 pKa = 5.35 RR324 pKa = 11.84 RR325 pKa = 11.84 WQWCVNGSHH334 pKa = 6.19 NRR336 pKa = 11.84 TWDD339 pKa = 2.93 ARR341 pKa = 11.84 AGVDD345 pKa = 4.95 LPASMPGCDD354 pKa = 2.73 RR355 pKa = 11.84 FYY357 pKa = 10.82 RR358 pKa = 11.84 RR359 pKa = 11.84 AFSEE363 pKa = 4.3 VCKK366 pKa = 11.01 VEE368 pKa = 4.13 TLTGWSGEE376 pKa = 4.1 VLAGVSPKK384 pKa = 10.68 LEE386 pKa = 4.2 NGKK389 pKa = 7.79 TRR391 pKa = 11.84 AIFACDD397 pKa = 3.19 TLSYY401 pKa = 11.01 YY402 pKa = 11.0 AFEE405 pKa = 4.82 HH406 pKa = 6.43 LLGPVSAAWLDD417 pKa = 3.56 RR418 pKa = 11.84 RR419 pKa = 11.84 VVLDD423 pKa = 3.77 PGRR426 pKa = 11.84 VGHH429 pKa = 7.2 LGMAEE434 pKa = 4.31 RR435 pKa = 11.84 INRR438 pKa = 11.84 TRR440 pKa = 11.84 DD441 pKa = 2.98 GGGIDD446 pKa = 4.48 VMLDD450 pKa = 3.21 YY451 pKa = 11.55 DD452 pKa = 4.93 DD453 pKa = 5.9 FNSHH457 pKa = 6.79 HH458 pKa = 7.48 SNTSMRR464 pKa = 11.84 ILLEE468 pKa = 4.11 EE469 pKa = 3.76 TCAAVGYY476 pKa = 10.27 DD477 pKa = 3.57 EE478 pKa = 4.55 EE479 pKa = 5.15 LGRR482 pKa = 11.84 KK483 pKa = 8.92 LCQSFDD489 pKa = 3.74 NTWVKK494 pKa = 9.83 TPAGLSRR501 pKa = 11.84 VRR503 pKa = 11.84 GTLMSGHH510 pKa = 7.15 RR511 pKa = 11.84 GTTYY515 pKa = 10.57 INSVLNAAYY524 pKa = 9.42 IRR526 pKa = 11.84 LAVGRR531 pKa = 11.84 AAYY534 pKa = 9.89 EE535 pKa = 4.36 GMVSMHH541 pKa = 6.61 VGDD544 pKa = 5.01 DD545 pKa = 3.93 VYY547 pKa = 11.73 VNCPTPEE554 pKa = 4.08 GVEE557 pKa = 4.09 EE558 pKa = 4.28 LVDD561 pKa = 3.46 RR562 pKa = 11.84 CAAIGCRR569 pKa = 11.84 MNPTKK574 pKa = 10.62 QSVGKK579 pKa = 10.53 VGAEE583 pKa = 3.97 FLRR586 pKa = 11.84 MGIRR590 pKa = 11.84 RR591 pKa = 11.84 EE592 pKa = 4.01 GAHH595 pKa = 6.75 GYY597 pKa = 9.08 LARR600 pKa = 11.84 SVASLVSGNWVNEE613 pKa = 3.7 KK614 pKa = 10.78 VMDD617 pKa = 4.44 PEE619 pKa = 4.36 EE620 pKa = 4.65 LLSSMVGTVRR630 pKa = 11.84 SLINRR635 pKa = 11.84 SGCEE639 pKa = 3.77 TVPQLLAPAVSAVTRR654 pKa = 11.84 IKK656 pKa = 10.55 VARR659 pKa = 11.84 CVSLLSGQSALEE671 pKa = 3.84 GRR673 pKa = 11.84 PVFNPRR679 pKa = 11.84 DD680 pKa = 3.52 GLIRR684 pKa = 11.84 TWALRR689 pKa = 11.84 VEE691 pKa = 4.63 RR692 pKa = 11.84 PASKK696 pKa = 10.64 VKK698 pKa = 10.93 AKK700 pKa = 10.29 DD701 pKa = 3.61 LPSNATDD708 pKa = 4.37 DD709 pKa = 4.4 YY710 pKa = 10.51 LAKK713 pKa = 10.29 AASVLEE719 pKa = 3.98 LRR721 pKa = 11.84 GAAMSTVDD729 pKa = 4.12 PRR731 pKa = 11.84 AAMLDD736 pKa = 3.17 SSYY739 pKa = 11.31 RR740 pKa = 11.84 KK741 pKa = 7.6 TLAGDD746 pKa = 3.7 TDD748 pKa = 3.91 PSKK751 pKa = 11.0 IKK753 pKa = 9.77 LTLRR757 pKa = 11.84 SCTPVLARR765 pKa = 11.84 GATNARR771 pKa = 11.84 DD772 pKa = 3.92 LLRR775 pKa = 11.84 RR776 pKa = 11.84 PCPPGALEE784 pKa = 5.36 RR785 pKa = 11.84 YY786 pKa = 8.62 PLLQLVKK793 pKa = 10.63 SGLRR797 pKa = 11.84 DD798 pKa = 3.19 SDD800 pKa = 3.36 VRR802 pKa = 11.84 EE803 pKa = 4.15 LVRR806 pKa = 11.84 EE807 pKa = 4.17 AGGDD811 pKa = 3.04 WTARR815 pKa = 11.84 DD816 pKa = 3.5 IGAEE820 pKa = 3.55 AWGIPSRR827 pKa = 11.84 SRR829 pKa = 11.84 AISGVASYY837 pKa = 11.05 TDD839 pKa = 3.39 AASLARR845 pKa = 11.84 RR846 pKa = 11.84 AEE848 pKa = 3.96 TDD850 pKa = 3.61 VVFFPYY856 pKa = 9.13 PVHH859 pKa = 5.96 MM860 pKa = 5.35
Molecular weight: 93.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.677
IPC2_protein 7.951
IPC_protein 7.761
Toseland 7.337
ProMoST 8.361
Dawson 8.492
Bjellqvist 8.902
Wikipedia 8.419
Rodwell 8.521
Grimsley 7.351
Solomon 8.551
Lehninger 8.565
Nozaki 9.121
DTASelect 8.595
Thurlkill 8.653
EMBOSS 8.697
Sillero 8.99
Patrickios 4.66
IPC_peptide 8.536
IPC2_peptide 7.936
IPC2.peptide.svr19 8.031
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1616
756
860
808.0
86.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.314 ± 0.881
1.795 ± 0.317
5.446 ± 0.163
5.631 ± 0.318
2.537 ± 0.251
10.025 ± 0.908
2.166 ± 0.412
2.661 ± 0.099
2.661 ± 0.633
10.025 ± 0.871
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.609 ± 0.459
2.29 ± 0.205
5.446 ± 0.43
1.98 ± 0.181
8.54 ± 0.583
6.931 ± 0.213
4.95 ± 0.585
9.282 ± 0.074
1.176 ± 0.435
2.537 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here