Marinobacter sp. B9-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; unclassified Marinobacter

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4DAS4|A0A2T4DAS4_9ALTE Cysteine methyltransferase (Fragment) OS=Marinobacter sp. B9-2 OX=2137198 GN=C9974_16790 PE=4 SV=1
SS1 pKa = 5.45TAGPIRR7 pKa = 11.84TDD9 pKa = 2.96GGEE12 pKa = 3.92EE13 pKa = 3.75LLAYY17 pKa = 8.96MPSHH21 pKa = 5.59VTGDD25 pKa = 3.98LNRR28 pKa = 11.84LADD31 pKa = 4.12PNYY34 pKa = 8.24SHH36 pKa = 6.92QYY38 pKa = 9.15YY39 pKa = 10.4VDD41 pKa = 4.06GPSTYY46 pKa = 11.21GDD48 pKa = 3.32VFFGNEE54 pKa = 3.13WHH56 pKa = 6.31TALVGALGHH65 pKa = 6.66GGQGLFALDD74 pKa = 3.05ITDD77 pKa = 3.71PKK79 pKa = 8.85GTQVGYY85 pKa = 9.84PSFSEE90 pKa = 4.29SDD92 pKa = 3.36ASDD95 pKa = 3.37LVLWEE100 pKa = 4.95FSDD103 pKa = 5.66ADD105 pKa = 4.18DD106 pKa = 5.28ADD108 pKa = 3.43MGYY111 pKa = 10.37SYY113 pKa = 11.13GDD115 pKa = 3.54PVIVRR120 pKa = 11.84MNNGKK125 pKa = 8.37WVAIFANGYY134 pKa = 10.38NNTDD138 pKa = 2.72SDD140 pKa = 4.74GNVSSTGNAVLYY152 pKa = 10.4IVDD155 pKa = 4.61IEE157 pKa = 4.3TGDD160 pKa = 4.1IIRR163 pKa = 11.84KK164 pKa = 9.31LDD166 pKa = 3.35TGVGVV171 pKa = 3.74

Molecular weight: 18.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4DGW1|A0A2T4DGW1_9ALTE Uncharacterized protein OS=Marinobacter sp. B9-2 OX=2137198 GN=C9974_10270 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.34VISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.41GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3190

0

3190

949678

22

1482

297.7

32.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.7 ± 0.045

0.916 ± 0.015

5.73 ± 0.036

6.736 ± 0.038

3.904 ± 0.028

7.848 ± 0.039

2.178 ± 0.022

5.33 ± 0.032

3.793 ± 0.035

10.653 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.019

3.291 ± 0.025

4.653 ± 0.027

3.926 ± 0.03

6.385 ± 0.036

5.983 ± 0.035

5.1 ± 0.03

7.279 ± 0.036

1.385 ± 0.017

2.602 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski