Pseudomonas phage vB_Pae-SS2019XII

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Beetrevirus; unclassified Beetrevirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2FA51|A0A5Q2FA51_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_Pae-SS2019XII OX=2660689 GN=vBPaeSS2019XII_044 PE=4 SV=1
MM1 pKa = 7.8ADD3 pKa = 3.55VLEE6 pKa = 4.72IDD8 pKa = 5.11CPACSTPYY16 pKa = 10.46PEE18 pKa = 4.11ITAGSAAHH26 pKa = 6.78DD27 pKa = 3.91PSLIEE32 pKa = 5.41LVITCSNCGHH42 pKa = 7.22ILNAFVSLAEE52 pKa = 4.12MSVVPNPEE60 pKa = 4.01EE61 pKa = 4.23EE62 pKa = 4.42TSHH65 pKa = 6.27GG66 pKa = 3.71

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F5V6|A0A5Q2F5V6_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_Pae-SS2019XII OX=2660689 GN=vBPaeSS2019XII_015 PE=4 SV=1
MM1 pKa = 7.24SGARR5 pKa = 11.84IEE7 pKa = 5.24LEE9 pKa = 3.8FDD11 pKa = 3.45SQQVTQALSAAAATLRR27 pKa = 11.84DD28 pKa = 3.55PSLILEE34 pKa = 4.52DD35 pKa = 5.07LIEE38 pKa = 4.51PLLRR42 pKa = 11.84IHH44 pKa = 6.05QARR47 pKa = 11.84FRR49 pKa = 11.84AQQAPDD55 pKa = 3.71GTPWTALSPRR65 pKa = 11.84YY66 pKa = 9.2LARR69 pKa = 11.84KK70 pKa = 8.69RR71 pKa = 11.84RR72 pKa = 11.84NRR74 pKa = 11.84DD75 pKa = 3.12KK76 pKa = 10.95ILTSEE81 pKa = 3.99GLLRR85 pKa = 11.84GSLRR89 pKa = 11.84GQVEE93 pKa = 3.99GDD95 pKa = 2.95ILLFGTDD102 pKa = 3.16RR103 pKa = 11.84PYY105 pKa = 11.49GAIHH109 pKa = 5.92QFGGTIQRR117 pKa = 11.84QEE119 pKa = 4.17KK120 pKa = 9.89QSTVYY125 pKa = 10.59FRR127 pKa = 11.84MNEE130 pKa = 3.75RR131 pKa = 11.84TGEE134 pKa = 4.08VGRR137 pKa = 11.84QFVPKK142 pKa = 9.85RR143 pKa = 11.84RR144 pKa = 11.84SNFAQDD150 pKa = 3.55VRR152 pKa = 11.84IGPYY156 pKa = 9.51TITMPARR163 pKa = 11.84PWLGTSDD170 pKa = 3.65TDD172 pKa = 5.07DD173 pKa = 3.66AQLLQRR179 pKa = 11.84VMSLINSTLQNN190 pKa = 3.34

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12714

47

1255

231.2

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.223 ± 0.662

0.889 ± 0.137

5.789 ± 0.231

6.418 ± 0.359

2.942 ± 0.137

7.834 ± 0.342

1.762 ± 0.19

4.318 ± 0.181

3.61 ± 0.317

9.91 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.139 ± 0.143

2.997 ± 0.217

4.821 ± 0.347

5.482 ± 0.345

7.401 ± 0.291

5.702 ± 0.21

5.262 ± 0.282

6.292 ± 0.343

1.605 ± 0.166

2.603 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski