Nordella sp. HKS 07

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Nordella; unclassified Nordella

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6466 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6WRF9|A0A6G6WRF9_9RHIZ Carbohydrate kinase OS=Nordella sp. HKS 07 OX=2712222 GN=G5V57_07830 PE=3 SV=1
MM1 pKa = 7.27NNAAKK6 pKa = 10.52DD7 pKa = 3.82DD8 pKa = 4.05VVGLNGDD15 pKa = 3.75FTFTIADD22 pKa = 4.66LLANDD27 pKa = 4.57PGGAAKK33 pKa = 10.88VNVTKK38 pKa = 10.74QFFFGNTSDD47 pKa = 4.12YY48 pKa = 11.57DD49 pKa = 3.72GLGANDD55 pKa = 3.91FGVVDD60 pKa = 5.2FAHH63 pKa = 6.79GGIPTVAQQTAYY75 pKa = 11.04LLAHH79 pKa = 7.24NITANADD86 pKa = 3.46FTEE89 pKa = 4.57FTIGAGGSDD98 pKa = 3.66IEE100 pKa = 4.53YY101 pKa = 9.51MVQIGNKK108 pKa = 7.54GTWSQADD115 pKa = 3.42VDD117 pKa = 4.25VTAPVPVPHH126 pKa = 6.65VGGNLFTEE134 pKa = 4.43NFDD137 pKa = 4.42GYY139 pKa = 11.11DD140 pKa = 3.46SNVQQTYY147 pKa = 10.53YY148 pKa = 11.25DD149 pKa = 4.1PADD152 pKa = 3.36STNAVFASVNLNNASGWTGAQNSEE176 pKa = 4.56LGADD180 pKa = 4.14GYY182 pKa = 11.73GGIKK186 pKa = 8.81ATSGGPDD193 pKa = 4.01GFWLDD198 pKa = 3.76TQNTPGQINISHH210 pKa = 7.62DD211 pKa = 3.88FTDD214 pKa = 3.15STAAVGGKK222 pKa = 7.42TAVLSFDD229 pKa = 3.26IAKK232 pKa = 10.57QNLTYY237 pKa = 10.69LGNAYY242 pKa = 7.84QTEE245 pKa = 4.43ANASFDD251 pKa = 3.59VRR253 pKa = 11.84IDD255 pKa = 3.31GVTVKK260 pKa = 10.1TILASDD266 pKa = 3.98LVEE269 pKa = 4.22NNQMYY274 pKa = 10.63HH275 pKa = 6.62FDD277 pKa = 3.84VDD279 pKa = 3.25IADD282 pKa = 3.99YY283 pKa = 11.33ADD285 pKa = 4.04NADD288 pKa = 3.7STHH291 pKa = 6.96TITLVDD297 pKa = 4.12TSPQADD303 pKa = 3.57FTGFSIDD310 pKa = 4.56SIQINDD316 pKa = 3.48WVVV319 pKa = 2.48

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6WPV8|A0A6G6WPV8_9RHIZ NAD(P)/FAD-dependent oxidoreductase OS=Nordella sp. HKS 07 OX=2712222 GN=G5V57_05060 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84RR3 pKa = 11.84AVKK6 pKa = 10.37RR7 pKa = 11.84KK8 pKa = 9.1AVKK11 pKa = 10.04GALKK15 pKa = 10.29RR16 pKa = 11.84RR17 pKa = 11.84AVKK20 pKa = 10.27KK21 pKa = 10.03AVVRR25 pKa = 11.84KK26 pKa = 8.68AVKK29 pKa = 9.92RR30 pKa = 11.84RR31 pKa = 11.84AVKK34 pKa = 10.15KK35 pKa = 9.71AVVRR39 pKa = 11.84KK40 pKa = 8.36AVKK43 pKa = 9.98RR44 pKa = 11.84RR45 pKa = 11.84VVKK48 pKa = 10.52KK49 pKa = 10.48AIARR53 pKa = 11.84KK54 pKa = 8.43ALKK57 pKa = 10.13RR58 pKa = 11.84RR59 pKa = 11.84AVKK62 pKa = 10.28KK63 pKa = 10.5AIARR67 pKa = 11.84KK68 pKa = 8.76AIKK71 pKa = 9.93RR72 pKa = 11.84AIVKK76 pKa = 10.02RR77 pKa = 11.84ALRR80 pKa = 11.84QAIVARR86 pKa = 11.84ALSEE90 pKa = 4.07TGGGSGSEE98 pKa = 4.04

Molecular weight: 10.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6466

0

6466

2011296

25

2426

311.1

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.098 ± 0.039

0.87 ± 0.01

5.615 ± 0.024

5.548 ± 0.025

3.852 ± 0.019

8.459 ± 0.034

2.051 ± 0.015

5.625 ± 0.022

3.818 ± 0.029

10.269 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.014

2.686 ± 0.018

5.094 ± 0.023

3.04 ± 0.015

6.904 ± 0.029

5.462 ± 0.022

5.242 ± 0.026

7.13 ± 0.02

1.422 ± 0.015

2.385 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski