Amino acid dipepetide frequency for Nordella sp. HKS 07

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.848AlaAla: 15.848 ± 0.127
1.062AlaCys: 1.062 ± 0.024
6.578AlaAsp: 6.578 ± 0.059
7.407AlaGlu: 7.407 ± 0.072
4.492AlaPhe: 4.492 ± 0.046
10.316AlaGly: 10.316 ± 0.083
2.313AlaHis: 2.313 ± 0.033
6.672AlaIle: 6.672 ± 0.061
4.697AlaLys: 4.697 ± 0.058
13.154AlaLeu: 13.154 ± 0.104
3.289AlaMet: 3.289 ± 0.045
2.858AlaAsn: 2.858 ± 0.038
5.098AlaPro: 5.098 ± 0.058
3.914AlaGln: 3.914 ± 0.048
8.627AlaArg: 8.627 ± 0.079
6.245AlaSer: 6.245 ± 0.063
5.792AlaThr: 5.792 ± 0.063
8.355AlaVal: 8.355 ± 0.08
1.516AlaTrp: 1.516 ± 0.028
2.746AlaTyr: 2.746 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
0.982CysAla: 0.982 ± 0.024
0.121CysCys: 0.121 ± 0.008
0.522CysAsp: 0.522 ± 0.019
0.445CysGlu: 0.445 ± 0.015
0.352CysPhe: 0.352 ± 0.013
0.911CysGly: 0.911 ± 0.025
0.266CysHis: 0.266 ± 0.012
0.434CysIle: 0.434 ± 0.016
0.198CysLys: 0.198 ± 0.009
0.854CysLeu: 0.854 ± 0.021
0.145CysMet: 0.145 ± 0.009
0.209CysAsn: 0.209 ± 0.01
0.45CysPro: 0.45 ± 0.017
0.232CysGln: 0.232 ± 0.011
0.707CysArg: 0.707 ± 0.019
0.485CysSer: 0.485 ± 0.016
0.427CysThr: 0.427 ± 0.014
0.612CysVal: 0.612 ± 0.016
0.124CysTrp: 0.124 ± 0.008
0.222CysTyr: 0.222 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
6.102AspAla: 6.102 ± 0.055
0.491AspCys: 0.491 ± 0.018
2.978AspAsp: 2.978 ± 0.056
3.431AspGlu: 3.431 ± 0.041
2.321AspPhe: 2.321 ± 0.041
4.953AspGly: 4.953 ± 0.067
1.226AspHis: 1.226 ± 0.027
3.437AspIle: 3.437 ± 0.046
2.142AspLys: 2.142 ± 0.04
5.838AspLeu: 5.838 ± 0.049
1.359AspMet: 1.359 ± 0.025
1.363AspAsn: 1.363 ± 0.03
3.387AspPro: 3.387 ± 0.046
1.769AspGln: 1.769 ± 0.028
4.015AspArg: 4.015 ± 0.048
2.2AspSer: 2.2 ± 0.036
2.482AspThr: 2.482 ± 0.041
4.056AspVal: 4.056 ± 0.055
1.023AspTrp: 1.023 ± 0.025
1.578AspTyr: 1.578 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.358GluAla: 7.358 ± 0.08
0.389GluCys: 0.389 ± 0.015
2.613GluAsp: 2.613 ± 0.035
3.092GluGlu: 3.092 ± 0.05
1.843GluPhe: 1.843 ± 0.029
4.025GluGly: 4.025 ± 0.045
1.111GluHis: 1.111 ± 0.023
3.674GluIle: 3.674 ± 0.046
2.513GluLys: 2.513 ± 0.04
5.38GluLeu: 5.38 ± 0.06
1.463GluMet: 1.463 ± 0.028
1.552GluAsn: 1.552 ± 0.027
2.764GluPro: 2.764 ± 0.043
1.883GluGln: 1.883 ± 0.032
4.698GluArg: 4.698 ± 0.06
2.366GluSer: 2.366 ± 0.035
3.433GluThr: 3.433 ± 0.043
3.646GluVal: 3.646 ± 0.052
0.697GluTrp: 0.697 ± 0.019
0.981GluTyr: 0.981 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
4.834PheAla: 4.834 ± 0.049
0.398PheCys: 0.398 ± 0.016
2.635PheAsp: 2.635 ± 0.044
2.087PheGlu: 2.087 ± 0.031
1.498PhePhe: 1.498 ± 0.033
3.657PheGly: 3.657 ± 0.047
0.771PheHis: 0.771 ± 0.018
2.013PheIle: 2.013 ± 0.039
1.175PheLys: 1.175 ± 0.026
3.659PheLeu: 3.659 ± 0.047
0.834PheMet: 0.834 ± 0.019
1.139PheAsn: 1.139 ± 0.027
1.661PhePro: 1.661 ± 0.029
1.019PheGln: 1.019 ± 0.025
2.247PheArg: 2.247 ± 0.037
2.241PheSer: 2.241 ± 0.034
2.252PheThr: 2.252 ± 0.037
2.852PheVal: 2.852 ± 0.04
0.632PheTrp: 0.632 ± 0.02
0.913PheTyr: 0.913 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
8.753GlyAla: 8.753 ± 0.087
0.813GlyCys: 0.813 ± 0.025
4.52GlyAsp: 4.52 ± 0.058
4.651GlyGlu: 4.651 ± 0.049
3.614GlyPhe: 3.614 ± 0.044
7.278GlyGly: 7.278 ± 0.119
1.891GlyHis: 1.891 ± 0.038
4.937GlyIle: 4.937 ± 0.061
3.781GlyLys: 3.781 ± 0.048
9.083GlyLeu: 9.083 ± 0.08
2.115GlyMet: 2.115 ± 0.039
2.357GlyAsn: 2.357 ± 0.046
3.442GlyPro: 3.442 ± 0.043
2.751GlyGln: 2.751 ± 0.041
5.939GlyArg: 5.939 ± 0.065
4.569GlySer: 4.569 ± 0.053
4.477GlyThr: 4.477 ± 0.061
5.803GlyVal: 5.803 ± 0.058
1.419GlyTrp: 1.419 ± 0.026
2.4GlyTyr: 2.4 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.155HisAla: 2.155 ± 0.037
0.266HisCys: 0.266 ± 0.011
1.217HisAsp: 1.217 ± 0.025
1.158HisGlu: 1.158 ± 0.024
0.92HisPhe: 0.92 ± 0.02
1.918HisGly: 1.918 ± 0.033
0.577HisHis: 0.577 ± 0.021
1.06HisIle: 1.06 ± 0.022
0.637HisLys: 0.637 ± 0.019
2.114HisLeu: 2.114 ± 0.036
0.521HisMet: 0.521 ± 0.016
0.478HisAsn: 0.478 ± 0.016
1.284HisPro: 1.284 ± 0.026
0.615HisGln: 0.615 ± 0.019
1.402HisArg: 1.402 ± 0.029
0.966HisSer: 0.966 ± 0.025
0.75HisThr: 0.75 ± 0.021
1.525HisVal: 1.525 ± 0.028
0.349HisTrp: 0.349 ± 0.014
0.601HisTyr: 0.601 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
7.945IleAla: 7.945 ± 0.075
0.585IleCys: 0.585 ± 0.016
3.826IleAsp: 3.826 ± 0.041
3.633IleGlu: 3.633 ± 0.047
1.956IlePhe: 1.956 ± 0.032
5.275IleGly: 5.275 ± 0.056
1.011IleHis: 1.011 ± 0.026
2.837IleIle: 2.837 ± 0.044
1.783IleLys: 1.783 ± 0.035
5.104IleLeu: 5.104 ± 0.054
1.043IleMet: 1.043 ± 0.025
1.511IleAsn: 1.511 ± 0.027
2.476IlePro: 2.476 ± 0.039
1.24IleGln: 1.24 ± 0.024
3.285IleArg: 3.285 ± 0.041
3.104IleSer: 3.104 ± 0.041
3.059IleThr: 3.059 ± 0.047
4.543IleVal: 4.543 ± 0.047
0.736IleTrp: 0.736 ± 0.02
1.298IleTyr: 1.298 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
4.964LysAla: 4.964 ± 0.062
0.213LysCys: 0.213 ± 0.01
2.129LysAsp: 2.129 ± 0.041
1.9LysGlu: 1.9 ± 0.034
1.082LysPhe: 1.082 ± 0.023
3.171LysGly: 3.171 ± 0.044
0.674LysHis: 0.674 ± 0.019
1.942LysIle: 1.942 ± 0.035
1.654LysLys: 1.654 ± 0.042
3.983LysLeu: 3.983 ± 0.049
0.856LysMet: 0.856 ± 0.021
1.013LysAsn: 1.013 ± 0.025
2.361LysPro: 2.361 ± 0.043
1.16LysGln: 1.16 ± 0.025
2.698LysArg: 2.698 ± 0.043
2.247LysSer: 2.247 ± 0.038
2.175LysThr: 2.175 ± 0.036
2.755LysVal: 2.755 ± 0.046
0.469LysTrp: 0.469 ± 0.018
0.729LysTyr: 0.729 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.093LeuAla: 14.093 ± 0.108
0.931LeuCys: 0.931 ± 0.021
5.989LeuAsp: 5.989 ± 0.055
4.979LeuGlu: 4.979 ± 0.05
3.795LeuPhe: 3.795 ± 0.05
8.449LeuGly: 8.449 ± 0.079
1.864LeuHis: 1.864 ± 0.035
5.802LeuIle: 5.802 ± 0.064
4.309LeuLys: 4.309 ± 0.052
9.917LeuLeu: 9.917 ± 0.099
2.302LeuMet: 2.302 ± 0.036
2.686LeuAsn: 2.686 ± 0.04
5.449LeuPro: 5.449 ± 0.053
2.609LeuGln: 2.609 ± 0.041
6.637LeuArg: 6.637 ± 0.063
6.544LeuSer: 6.544 ± 0.071
5.601LeuThr: 5.601 ± 0.057
7.258LeuVal: 7.258 ± 0.071
1.266LeuTrp: 1.266 ± 0.026
2.212LeuTyr: 2.212 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
3.044MetAla: 3.044 ± 0.037
0.147MetCys: 0.147 ± 0.009
1.036MetAsp: 1.036 ± 0.022
1.006MetGlu: 1.006 ± 0.025
0.694MetPhe: 0.694 ± 0.019
1.728MetGly: 1.728 ± 0.029
0.419MetHis: 0.419 ± 0.014
1.418MetIle: 1.418 ± 0.022
1.125MetLys: 1.125 ± 0.026
2.394MetLeu: 2.394 ± 0.038
0.664MetMet: 0.664 ± 0.02
0.743MetAsn: 0.743 ± 0.019
1.452MetPro: 1.452 ± 0.03
0.778MetGln: 0.778 ± 0.021
1.853MetArg: 1.853 ± 0.03
1.725MetSer: 1.725 ± 0.031
1.89MetThr: 1.89 ± 0.029
1.591MetVal: 1.591 ± 0.03
0.262MetTrp: 0.262 ± 0.013
0.333MetTyr: 0.333 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.036AsnAla: 3.036 ± 0.035
0.286AsnCys: 0.286 ± 0.013
1.467AsnAsp: 1.467 ± 0.035
1.341AsnGlu: 1.341 ± 0.026
1.078AsnPhe: 1.078 ± 0.024
2.382AsnGly: 2.382 ± 0.044
0.51AsnHis: 0.51 ± 0.016
1.52AsnIle: 1.52 ± 0.025
0.839AsnLys: 0.839 ± 0.021
2.659AsnLeu: 2.659 ± 0.037
0.693AsnMet: 0.693 ± 0.018
0.78AsnAsn: 0.78 ± 0.024
1.864AsnPro: 1.864 ± 0.031
0.753AsnGln: 0.753 ± 0.017
1.74AsnArg: 1.74 ± 0.03
1.387AsnSer: 1.387 ± 0.033
1.222AsnThr: 1.222 ± 0.022
2.03AsnVal: 2.03 ± 0.037
0.498AsnTrp: 0.498 ± 0.016
0.777AsnTyr: 0.777 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
5.809ProAla: 5.809 ± 0.069
0.287ProCys: 0.287 ± 0.011
3.528ProAsp: 3.528 ± 0.05
3.61ProGlu: 3.61 ± 0.05
2.041ProPhe: 2.041 ± 0.036
4.475ProGly: 4.475 ± 0.053
1.149ProHis: 1.149 ± 0.023
2.423ProIle: 2.423 ± 0.036
1.966ProLys: 1.966 ± 0.036
4.735ProLeu: 4.735 ± 0.05
1.168ProMet: 1.168 ± 0.024
1.377ProAsn: 1.377 ± 0.028
2.58ProPro: 2.58 ± 0.053
1.711ProGln: 1.711 ± 0.031
2.904ProArg: 2.904 ± 0.043
2.753ProSer: 2.753 ± 0.04
2.334ProThr: 2.334 ± 0.042
4.027ProVal: 4.027 ± 0.048
0.723ProTrp: 0.723 ± 0.021
1.336ProTyr: 1.336 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.913GlnAla: 3.913 ± 0.042
0.241GlnCys: 0.241 ± 0.012
1.425GlnAsp: 1.425 ± 0.028
1.414GlnGlu: 1.414 ± 0.029
1.091GlnPhe: 1.091 ± 0.024
2.526GlnGly: 2.526 ± 0.032
0.589GlnHis: 0.589 ± 0.016
1.832GlnIle: 1.832 ± 0.028
1.184GlnLys: 1.184 ± 0.025
2.881GlnLeu: 2.881 ± 0.039
0.862GlnMet: 0.862 ± 0.019
0.869GlnAsn: 0.869 ± 0.023
1.584GlnPro: 1.584 ± 0.035
1.111GlnGln: 1.111 ± 0.028
2.25GlnArg: 2.25 ± 0.033
1.8GlnSer: 1.8 ± 0.027
1.618GlnThr: 1.618 ± 0.031
2.088GlnVal: 2.088 ± 0.035
0.462GlnTrp: 0.462 ± 0.015
0.658GlnTyr: 0.658 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
7.318ArgAla: 7.318 ± 0.068
0.571ArgCys: 0.571 ± 0.019
3.908ArgAsp: 3.908 ± 0.041
3.955ArgGlu: 3.955 ± 0.051
2.817ArgPhe: 2.817 ± 0.038
4.497ArgGly: 4.497 ± 0.052
1.78ArgHis: 1.78 ± 0.032
4.199ArgIle: 4.199 ± 0.047
2.792ArgLys: 2.792 ± 0.044
7.891ArgLeu: 7.891 ± 0.073
1.786ArgMet: 1.786 ± 0.031
2.05ArgAsn: 2.05 ± 0.037
3.441ArgPro: 3.441 ± 0.047
2.647ArgGln: 2.647 ± 0.037
5.701ArgArg: 5.701 ± 0.072
3.63ArgSer: 3.63 ± 0.041
3.02ArgThr: 3.02 ± 0.035
4.277ArgVal: 4.277 ± 0.043
0.961ArgTrp: 0.961 ± 0.021
1.801ArgTyr: 1.801 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
6.018SerAla: 6.018 ± 0.066
0.486SerCys: 0.486 ± 0.018
3.028SerAsp: 3.028 ± 0.038
2.848SerGlu: 2.848 ± 0.043
2.554SerPhe: 2.554 ± 0.037
5.597SerGly: 5.597 ± 0.062
1.195SerHis: 1.195 ± 0.027
2.898SerIle: 2.898 ± 0.038
1.742SerLys: 1.742 ± 0.031
5.839SerLeu: 5.839 ± 0.065
1.3SerMet: 1.3 ± 0.026
1.344SerAsn: 1.344 ± 0.025
2.971SerPro: 2.971 ± 0.041
1.55SerGln: 1.55 ± 0.027
3.617SerArg: 3.617 ± 0.044
2.977SerSer: 2.977 ± 0.05
2.564SerThr: 2.564 ± 0.039
3.801SerVal: 3.801 ± 0.044
0.863SerTrp: 0.863 ± 0.02
1.433SerTyr: 1.433 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
6.12ThrAla: 6.12 ± 0.062
0.402ThrCys: 0.402 ± 0.015
2.588ThrAsp: 2.588 ± 0.044
2.51ThrGlu: 2.51 ± 0.04
2.029ThrPhe: 2.029 ± 0.037
5.072ThrGly: 5.072 ± 0.071
1.05ThrHis: 1.05 ± 0.024
3.109ThrIle: 3.109 ± 0.049
1.552ThrLys: 1.552 ± 0.03
5.804ThrLeu: 5.804 ± 0.064
1.176ThrMet: 1.176 ± 0.025
1.34ThrAsn: 1.34 ± 0.027
3.114ThrPro: 3.114 ± 0.043
1.496ThrGln: 1.496 ± 0.031
3.294ThrArg: 3.294 ± 0.045
2.873ThrSer: 2.873 ± 0.044
2.753ThrThr: 2.753 ± 0.042
4.171ThrVal: 4.171 ± 0.058
0.668ThrTrp: 0.668 ± 0.017
1.296ThrTyr: 1.296 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
8.679ValAla: 8.679 ± 0.079
0.604ValCys: 0.604 ± 0.018
3.922ValAsp: 3.922 ± 0.051
4.124ValGlu: 4.124 ± 0.047
2.718ValPhe: 2.718 ± 0.041
5.195ValGly: 5.195 ± 0.059
1.265ValHis: 1.265 ± 0.025
4.298ValIle: 4.298 ± 0.047
2.801ValLys: 2.801 ± 0.037
7.265ValLeu: 7.265 ± 0.074
1.847ValMet: 1.847 ± 0.031
2.041ValAsn: 2.041 ± 0.036
3.58ValPro: 3.58 ± 0.048
1.829ValGln: 1.829 ± 0.031
4.382ValArg: 4.382 ± 0.045
4.312ValSer: 4.312 ± 0.047
4.658ValThr: 4.658 ± 0.064
5.371ValVal: 5.371 ± 0.063
0.887ValTrp: 0.887 ± 0.023
1.523ValTyr: 1.523 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.354TrpAla: 1.354 ± 0.027
0.148TrpCys: 0.148 ± 0.008
0.689TrpAsp: 0.689 ± 0.019
0.585TrpGlu: 0.585 ± 0.019
0.582TrpPhe: 0.582 ± 0.018
0.989TrpGly: 0.989 ± 0.026
0.345TrpHis: 0.345 ± 0.012
0.767TrpIle: 0.767 ± 0.02
0.544TrpLys: 0.544 ± 0.018
1.694TrpLeu: 1.694 ± 0.032
0.393TrpMet: 0.393 ± 0.012
0.511TrpAsn: 0.511 ± 0.016
0.796TrpPro: 0.796 ± 0.021
0.565TrpGln: 0.565 ± 0.017
1.191TrpArg: 1.191 ± 0.027
0.897TrpSer: 0.897 ± 0.02
0.81TrpThr: 0.81 ± 0.022
0.804TrpVal: 0.804 ± 0.023
0.238TrpTrp: 0.238 ± 0.012
0.322TrpTyr: 0.322 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.655TyrAla: 2.655 ± 0.039
0.256TyrCys: 0.256 ± 0.012
1.551TyrAsp: 1.551 ± 0.031
1.305TyrGlu: 1.305 ± 0.028
1.038TyrPhe: 1.038 ± 0.023
2.178TyrGly: 2.178 ± 0.036
0.508TyrHis: 0.508 ± 0.016
0.981TyrIle: 0.981 ± 0.023
0.741TyrLys: 0.741 ± 0.018
2.445TyrLeu: 2.445 ± 0.039
0.486TyrMet: 0.486 ± 0.016
0.642TyrAsn: 0.642 ± 0.017
1.182TyrPro: 1.182 ± 0.024
0.766TyrGln: 0.766 ± 0.019
1.855TyrArg: 1.855 ± 0.033
1.305TyrSer: 1.305 ± 0.028
1.099TyrThr: 1.099 ± 0.028
1.735TyrVal: 1.735 ± 0.029
0.43TyrTrp: 0.43 ± 0.015
0.693TyrTyr: 0.693 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6466 proteins (2011296 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski