Bacillus sp. VT-16-64

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4399 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2S974|A0A1V2S974_9BACI Zn-dependent hydrolase (Fragment) OS=Bacillus sp. VT-16-64 OX=1917019 GN=BLX87_22140 PE=4 SV=1
MM1 pKa = 8.12PYY3 pKa = 9.94PVEE6 pKa = 3.99NPMVLDD12 pKa = 4.29HH13 pKa = 6.89LWDD16 pKa = 4.27DD17 pKa = 4.23AEE19 pKa = 4.69KK20 pKa = 11.01VWGTDD25 pKa = 2.98AMGDD29 pKa = 3.65EE30 pKa = 4.49IMEE33 pKa = 4.17GDD35 pKa = 4.82AIIEE39 pKa = 3.84IDD41 pKa = 3.84GEE43 pKa = 4.57VVLRR47 pKa = 11.84SNLEE51 pKa = 4.15DD52 pKa = 3.75YY53 pKa = 10.9LVEE56 pKa = 4.1YY57 pKa = 9.55YY58 pKa = 9.74QAHH61 pKa = 5.75FTYY64 pKa = 9.33ATT66 pKa = 3.42

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2S8R7|A0A1V2S8R7_9BACI Exodeoxyribonuclease III OS=Bacillus sp. VT-16-64 OX=1917019 GN=BLX87_23905 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.72RR12 pKa = 11.84SKK14 pKa = 9.03VHH16 pKa = 5.6GFRR19 pKa = 11.84VRR21 pKa = 11.84MSTKK25 pKa = 7.93TGRR28 pKa = 11.84RR29 pKa = 11.84ILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.84GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4399

0

4399

1221676

25

2205

277.7

31.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.361 ± 0.035

0.763 ± 0.012

4.961 ± 0.028

7.41 ± 0.048

4.622 ± 0.03

7.072 ± 0.04

2.248 ± 0.017

7.848 ± 0.038

6.901 ± 0.035

9.723 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.953 ± 0.019

4.016 ± 0.025

3.82 ± 0.027

3.6 ± 0.024

4.344 ± 0.03

5.785 ± 0.028

5.228 ± 0.022

6.935 ± 0.027

1.047 ± 0.014

3.362 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski