Amino acid dipepetide frequency for Bacillus sp. VT-16-64

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.63AlaAla: 6.63 ± 0.088
0.707AlaCys: 0.707 ± 0.027
3.689AlaAsp: 3.689 ± 0.058
4.867AlaGlu: 4.867 ± 0.072
3.512AlaPhe: 3.512 ± 0.052
5.861AlaGly: 5.861 ± 0.087
1.356AlaHis: 1.356 ± 0.032
6.133AlaIle: 6.133 ± 0.07
4.787AlaLys: 4.787 ± 0.068
7.52AlaLeu: 7.52 ± 0.079
2.086AlaMet: 2.086 ± 0.043
2.736AlaAsn: 2.736 ± 0.046
2.24AlaPro: 2.24 ± 0.048
2.172AlaGln: 2.172 ± 0.048
3.083AlaArg: 3.083 ± 0.05
4.066AlaSer: 4.066 ± 0.068
3.471AlaThr: 3.471 ± 0.057
5.745AlaVal: 5.745 ± 0.073
0.629AlaTrp: 0.629 ± 0.022
2.318AlaTyr: 2.318 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.502CysAla: 0.502 ± 0.02
0.088CysCys: 0.088 ± 0.008
0.376CysAsp: 0.376 ± 0.019
0.462CysGlu: 0.462 ± 0.022
0.404CysPhe: 0.404 ± 0.017
0.747CysGly: 0.747 ± 0.027
0.23CysHis: 0.23 ± 0.015
0.562CysIle: 0.562 ± 0.018
0.416CysLys: 0.416 ± 0.021
0.744CysLeu: 0.744 ± 0.026
0.196CysMet: 0.196 ± 0.012
0.291CysAsn: 0.291 ± 0.015
0.338CysPro: 0.338 ± 0.016
0.25CysGln: 0.25 ± 0.014
0.318CysArg: 0.318 ± 0.017
0.515CysSer: 0.515 ± 0.019
0.395CysThr: 0.395 ± 0.02
0.457CysVal: 0.457 ± 0.017
0.079CysTrp: 0.079 ± 0.008
0.257CysTyr: 0.257 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.157AspAla: 3.157 ± 0.049
0.373AspCys: 0.373 ± 0.018
2.391AspAsp: 2.391 ± 0.047
4.282AspGlu: 4.282 ± 0.066
2.323AspPhe: 2.323 ± 0.042
3.43AspGly: 3.43 ± 0.062
1.32AspHis: 1.32 ± 0.035
4.198AspIle: 4.198 ± 0.063
2.973AspLys: 2.973 ± 0.054
4.773AspLeu: 4.773 ± 0.057
1.487AspMet: 1.487 ± 0.036
1.566AspAsn: 1.566 ± 0.044
2.168AspPro: 2.168 ± 0.046
2.005AspGln: 2.005 ± 0.04
2.387AspArg: 2.387 ± 0.047
2.378AspSer: 2.378 ± 0.048
2.178AspThr: 2.178 ± 0.048
3.627AspVal: 3.627 ± 0.052
0.625AspTrp: 0.625 ± 0.027
1.971AspTyr: 1.971 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
5.516GluAla: 5.516 ± 0.083
0.413GluCys: 0.413 ± 0.017
3.449GluAsp: 3.449 ± 0.053
6.776GluGlu: 6.776 ± 0.102
2.426GluPhe: 2.426 ± 0.045
4.305GluGly: 4.305 ± 0.07
1.598GluHis: 1.598 ± 0.031
5.336GluIle: 5.336 ± 0.071
6.992GluLys: 6.992 ± 0.094
6.895GluLeu: 6.895 ± 0.082
2.465GluMet: 2.465 ± 0.043
3.49GluAsn: 3.49 ± 0.065
2.145GluPro: 2.145 ± 0.043
3.435GluGln: 3.435 ± 0.051
3.733GluArg: 3.733 ± 0.061
3.521GluSer: 3.521 ± 0.053
3.782GluThr: 3.782 ± 0.059
4.822GluVal: 4.822 ± 0.072
0.836GluTrp: 0.836 ± 0.03
2.168GluTyr: 2.168 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.085PheAla: 3.085 ± 0.052
0.4PheCys: 0.4 ± 0.017
2.223PheAsp: 2.223 ± 0.038
2.669PheGlu: 2.669 ± 0.053
2.644PhePhe: 2.644 ± 0.067
3.406PheGly: 3.406 ± 0.058
1.174PheHis: 1.174 ± 0.036
4.164PheIle: 4.164 ± 0.076
2.231PheLys: 2.231 ± 0.041
4.813PheLeu: 4.813 ± 0.068
1.309PheMet: 1.309 ± 0.033
1.577PheAsn: 1.577 ± 0.034
1.819PhePro: 1.819 ± 0.041
1.751PheGln: 1.751 ± 0.038
1.654PheArg: 1.654 ± 0.036
3.34PheSer: 3.34 ± 0.057
2.606PheThr: 2.606 ± 0.053
3.158PheVal: 3.158 ± 0.059
0.48PheTrp: 0.48 ± 0.024
1.715PheTyr: 1.715 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
5.168GlyAla: 5.168 ± 0.081
0.662GlyCys: 0.662 ± 0.025
3.161GlyAsp: 3.161 ± 0.056
4.76GlyGlu: 4.76 ± 0.087
3.428GlyPhe: 3.428 ± 0.059
5.234GlyGly: 5.234 ± 0.095
1.522GlyHis: 1.522 ± 0.038
5.978GlyIle: 5.978 ± 0.078
5.355GlyLys: 5.355 ± 0.069
6.745GlyLeu: 6.745 ± 0.077
2.315GlyMet: 2.315 ± 0.044
2.667GlyAsn: 2.667 ± 0.057
1.97GlyPro: 1.97 ± 0.045
2.187GlyGln: 2.187 ± 0.042
2.937GlyArg: 2.937 ± 0.052
3.957GlySer: 3.957 ± 0.06
4.085GlyThr: 4.085 ± 0.067
4.976GlyVal: 4.976 ± 0.075
0.916GlyTrp: 0.916 ± 0.028
2.702GlyTyr: 2.702 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.356HisAla: 1.356 ± 0.035
0.23HisCys: 0.23 ± 0.013
1.148HisAsp: 1.148 ± 0.03
1.529HisGlu: 1.529 ± 0.038
1.219HisPhe: 1.219 ± 0.034
1.449HisGly: 1.449 ± 0.034
0.747HisHis: 0.747 ± 0.027
1.819HisIle: 1.819 ± 0.047
1.125HisLys: 1.125 ± 0.032
2.345HisLeu: 2.345 ± 0.05
0.62HisMet: 0.62 ± 0.024
0.702HisAsn: 0.702 ± 0.027
1.253HisPro: 1.253 ± 0.033
0.853HisGln: 0.853 ± 0.026
0.96HisArg: 0.96 ± 0.027
1.302HisSer: 1.302 ± 0.036
1.041HisThr: 1.041 ± 0.028
1.65HisVal: 1.65 ± 0.039
0.219HisTrp: 0.219 ± 0.013
0.917HisTyr: 0.917 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.228IleAla: 6.228 ± 0.091
0.675IleCys: 0.675 ± 0.025
4.085IleAsp: 4.085 ± 0.052
5.356IleGlu: 5.356 ± 0.075
3.606IlePhe: 3.606 ± 0.064
6.053IleGly: 6.053 ± 0.076
1.965IleHis: 1.965 ± 0.041
5.952IleIle: 5.952 ± 0.083
4.446IleLys: 4.446 ± 0.064
7.089IleLeu: 7.089 ± 0.103
1.987IleMet: 1.987 ± 0.041
2.919IleAsn: 2.919 ± 0.058
3.621IlePro: 3.621 ± 0.055
3.025IleGln: 3.025 ± 0.051
3.407IleArg: 3.407 ± 0.05
5.016IleSer: 5.016 ± 0.063
4.188IleThr: 4.188 ± 0.064
5.8IleVal: 5.8 ± 0.078
0.683IleTrp: 0.683 ± 0.027
2.384IleTyr: 2.384 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.921LysAla: 4.921 ± 0.071
0.327LysCys: 0.327 ± 0.018
3.634LysAsp: 3.634 ± 0.066
6.864LysGlu: 6.864 ± 0.088
1.775LysPhe: 1.775 ± 0.04
4.445LysGly: 4.445 ± 0.062
1.432LysHis: 1.432 ± 0.033
4.535LysIle: 4.535 ± 0.067
6.284LysLys: 6.284 ± 0.083
5.627LysLeu: 5.627 ± 0.072
2.321LysMet: 2.321 ± 0.047
3.257LysAsn: 3.257 ± 0.051
2.388LysPro: 2.388 ± 0.057
2.993LysGln: 2.993 ± 0.06
3.597LysArg: 3.597 ± 0.055
3.44LysSer: 3.44 ± 0.055
3.705LysThr: 3.705 ± 0.052
4.558LysVal: 4.558 ± 0.068
0.819LysTrp: 0.819 ± 0.028
2.091LysTyr: 2.091 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
7.506LeuAla: 7.506 ± 0.087
0.68LeuCys: 0.68 ± 0.023
4.773LeuAsp: 4.773 ± 0.079
6.724LeuGlu: 6.724 ± 0.085
5.095LeuPhe: 5.095 ± 0.087
6.544LeuGly: 6.544 ± 0.08
2.168LeuHis: 2.168 ± 0.043
7.139LeuIle: 7.139 ± 0.1
6.494LeuLys: 6.494 ± 0.075
10.438LeuLeu: 10.438 ± 0.136
2.591LeuMet: 2.591 ± 0.048
3.758LeuAsn: 3.758 ± 0.053
4.082LeuPro: 4.082 ± 0.064
3.614LeuGln: 3.614 ± 0.061
3.815LeuArg: 3.815 ± 0.069
6.485LeuSer: 6.485 ± 0.086
5.259LeuThr: 5.259 ± 0.07
6.079LeuVal: 6.079 ± 0.069
0.834LeuTrp: 0.834 ± 0.028
3.147LeuTyr: 3.147 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.386MetAla: 2.386 ± 0.044
0.153MetCys: 0.153 ± 0.011
1.482MetAsp: 1.482 ± 0.031
2.287MetGlu: 2.287 ± 0.047
1.13MetPhe: 1.13 ± 0.036
1.972MetGly: 1.972 ± 0.037
0.442MetHis: 0.442 ± 0.02
2.335MetIle: 2.335 ± 0.049
2.663MetLys: 2.663 ± 0.046
2.757MetLeu: 2.757 ± 0.049
0.969MetMet: 0.969 ± 0.029
1.585MetAsn: 1.585 ± 0.034
1.027MetPro: 1.027 ± 0.033
0.825MetGln: 0.825 ± 0.028
1.265MetArg: 1.265 ± 0.029
1.756MetSer: 1.756 ± 0.038
1.721MetThr: 1.721 ± 0.036
1.776MetVal: 1.776 ± 0.036
0.217MetTrp: 0.217 ± 0.014
0.787MetTyr: 0.787 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
2.726AsnAla: 2.726 ± 0.043
0.294AsnCys: 0.294 ± 0.014
2.046AsnAsp: 2.046 ± 0.042
3.289AsnGlu: 3.289 ± 0.049
1.326AsnPhe: 1.326 ± 0.034
3.192AsnGly: 3.192 ± 0.058
1.016AsnHis: 1.016 ± 0.03
3.458AsnIle: 3.458 ± 0.051
2.677AsnLys: 2.677 ± 0.046
3.255AsnLeu: 3.255 ± 0.048
1.261AsnMet: 1.261 ± 0.03
1.643AsnAsn: 1.643 ± 0.039
2.018AsnPro: 2.018 ± 0.037
1.507AsnGln: 1.507 ± 0.035
2.014AsnArg: 2.014 ± 0.044
1.947AsnSer: 1.947 ± 0.043
1.97AsnThr: 1.97 ± 0.042
2.845AsnVal: 2.845 ± 0.054
0.504AsnTrp: 0.504 ± 0.022
1.177AsnTyr: 1.177 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
2.727ProAla: 2.727 ± 0.051
0.25ProCys: 0.25 ± 0.013
2.19ProAsp: 2.19 ± 0.039
3.113ProGlu: 3.113 ± 0.055
2.199ProPhe: 2.199 ± 0.042
2.755ProGly: 2.755 ± 0.057
0.918ProHis: 0.918 ± 0.03
2.874ProIle: 2.874 ± 0.044
2.276ProLys: 2.276 ± 0.04
3.806ProLeu: 3.806 ± 0.063
0.849ProMet: 0.849 ± 0.024
1.563ProAsn: 1.563 ± 0.037
1.299ProPro: 1.299 ± 0.038
1.139ProGln: 1.139 ± 0.035
1.291ProArg: 1.291 ± 0.036
2.253ProSer: 2.253 ± 0.043
1.826ProThr: 1.826 ± 0.038
3.039ProVal: 3.039 ± 0.049
0.4ProTrp: 0.4 ± 0.021
1.432ProTyr: 1.432 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.711GlnAla: 2.711 ± 0.052
0.214GlnCys: 0.214 ± 0.014
1.528GlnAsp: 1.528 ± 0.043
2.714GlnGlu: 2.714 ± 0.05
1.655GlnPhe: 1.655 ± 0.041
2.004GlnGly: 2.004 ± 0.038
0.844GlnHis: 0.844 ± 0.027
2.477GlnIle: 2.477 ± 0.048
2.772GlnLys: 2.772 ± 0.041
3.93GlnLeu: 3.93 ± 0.064
1.227GlnMet: 1.227 ± 0.028
1.496GlnAsn: 1.496 ± 0.036
1.235GlnPro: 1.235 ± 0.033
1.583GlnGln: 1.583 ± 0.042
1.559GlnArg: 1.559 ± 0.04
2.121GlnSer: 2.121 ± 0.04
1.987GlnThr: 1.987 ± 0.048
2.258GlnVal: 2.258 ± 0.041
0.386GlnTrp: 0.386 ± 0.016
1.297GlnTyr: 1.297 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.817ArgAla: 2.817 ± 0.052
0.329ArgCys: 0.329 ± 0.018
2.109ArgAsp: 2.109 ± 0.045
3.47ArgGlu: 3.47 ± 0.066
2.075ArgPhe: 2.075 ± 0.04
2.577ArgGly: 2.577 ± 0.048
0.985ArgHis: 0.985 ± 0.029
3.153ArgIle: 3.153 ± 0.058
3.599ArgLys: 3.599 ± 0.055
4.464ArgLeu: 4.464 ± 0.063
1.387ArgMet: 1.387 ± 0.038
1.914ArgAsn: 1.914 ± 0.04
1.542ArgPro: 1.542 ± 0.039
1.655ArgGln: 1.655 ± 0.04
2.155ArgArg: 2.155 ± 0.051
2.376ArgSer: 2.376 ± 0.049
2.141ArgThr: 2.141 ± 0.045
2.672ArgVal: 2.672 ± 0.053
0.488ArgTrp: 0.488 ± 0.02
1.536ArgTyr: 1.536 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
3.99SerAla: 3.99 ± 0.057
0.434SerCys: 0.434 ± 0.019
2.68SerAsp: 2.68 ± 0.039
3.719SerGlu: 3.719 ± 0.057
3.27SerPhe: 3.27 ± 0.06
4.765SerGly: 4.765 ± 0.072
1.283SerHis: 1.283 ± 0.031
4.861SerIle: 4.861 ± 0.064
3.453SerLys: 3.453 ± 0.055
5.947SerLeu: 5.947 ± 0.075
1.795SerMet: 1.795 ± 0.04
2.15SerAsn: 2.15 ± 0.042
2.216SerPro: 2.216 ± 0.041
1.78SerGln: 1.78 ± 0.041
2.44SerArg: 2.44 ± 0.047
3.543SerSer: 3.543 ± 0.065
2.861SerThr: 2.861 ± 0.053
4.032SerVal: 4.032 ± 0.06
0.625SerTrp: 0.625 ± 0.025
2.001SerTyr: 2.001 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.309ThrAla: 4.309 ± 0.065
0.391ThrCys: 0.391 ± 0.018
2.664ThrAsp: 2.664 ± 0.053
3.278ThrGlu: 3.278 ± 0.06
2.665ThrPhe: 2.665 ± 0.051
4.266ThrGly: 4.266 ± 0.064
1.002ThrHis: 1.002 ± 0.028
4.422ThrIle: 4.422 ± 0.064
3.139ThrLys: 3.139 ± 0.056
4.997ThrLeu: 4.997 ± 0.059
1.377ThrMet: 1.377 ± 0.037
2.196ThrAsn: 2.196 ± 0.049
2.264ThrPro: 2.264 ± 0.044
1.249ThrGln: 1.249 ± 0.036
1.891ThrArg: 1.891 ± 0.04
2.91ThrSer: 2.91 ± 0.056
2.711ThrThr: 2.711 ± 0.06
4.242ThrVal: 4.242 ± 0.062
0.503ThrTrp: 0.503 ± 0.023
1.807ThrTyr: 1.807 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
5.13ValAla: 5.13 ± 0.075
0.603ValCys: 0.603 ± 0.021
3.59ValAsp: 3.59 ± 0.059
4.727ValGlu: 4.727 ± 0.078
3.209ValPhe: 3.209 ± 0.051
4.568ValGly: 4.568 ± 0.071
1.409ValHis: 1.409 ± 0.032
5.742ValIle: 5.742 ± 0.077
4.578ValLys: 4.578 ± 0.062
6.743ValLeu: 6.743 ± 0.082
2.063ValMet: 2.063 ± 0.05
2.909ValAsn: 2.909 ± 0.054
2.795ValPro: 2.795 ± 0.047
2.362ValGln: 2.362 ± 0.046
2.817ValArg: 2.817 ± 0.055
4.373ValSer: 4.373 ± 0.059
4.002ValThr: 4.002 ± 0.07
4.829ValVal: 4.829 ± 0.069
0.684ValTrp: 0.684 ± 0.026
2.222ValTyr: 2.222 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.645TrpAla: 0.645 ± 0.023
0.093TrpCys: 0.093 ± 0.01
0.485TrpAsp: 0.485 ± 0.022
0.741TrpGlu: 0.741 ± 0.026
0.497TrpPhe: 0.497 ± 0.02
0.698TrpGly: 0.698 ± 0.025
0.21TrpHis: 0.21 ± 0.013
0.85TrpIle: 0.85 ± 0.031
0.747TrpLys: 0.747 ± 0.026
1.197TrpLeu: 1.197 ± 0.031
0.343TrpMet: 0.343 ± 0.018
0.548TrpAsn: 0.548 ± 0.026
0.34TrpPro: 0.34 ± 0.015
0.348TrpGln: 0.348 ± 0.018
0.464TrpArg: 0.464 ± 0.022
0.57TrpSer: 0.57 ± 0.022
0.588TrpThr: 0.588 ± 0.026
0.621TrpVal: 0.621 ± 0.026
0.147TrpTrp: 0.147 ± 0.012
0.336TrpTyr: 0.336 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.1TyrAla: 2.1 ± 0.041
0.309TyrCys: 0.309 ± 0.015
1.91TyrAsp: 1.91 ± 0.042
2.475TyrGlu: 2.475 ± 0.049
1.762TyrPhe: 1.762 ± 0.044
2.456TyrGly: 2.456 ± 0.048
0.862TyrHis: 0.862 ± 0.029
2.497TyrIle: 2.497 ± 0.048
2.002TyrLys: 2.002 ± 0.048
3.142TyrLeu: 3.142 ± 0.057
0.886TyrMet: 0.886 ± 0.027
1.195TyrAsn: 1.195 ± 0.033
1.439TyrPro: 1.439 ± 0.036
1.266TyrGln: 1.266 ± 0.036
1.658TyrArg: 1.658 ± 0.041
1.978TyrSer: 1.978 ± 0.042
1.766TyrThr: 1.766 ± 0.04
2.167TyrVal: 2.167 ± 0.041
0.394TyrTrp: 0.394 ± 0.019
1.352TyrTyr: 1.352 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4399 proteins (1221676 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski